Article
Multidisciplinary Sciences
Agustina Pascual, Rolando Rivera-Pomar
Summary: The study analyzes the maternal gene expression in R. prolixus during oogenesis and early embryogenesis and proposes three times of action for maternal genes.
SCIENTIFIC REPORTS
(2022)
Article
Entomology
Raquel A. Ferreira, Marcelo G. Lorenzo, Claudio R. Lazzari
Summary: Kissing bugs can estimate the distance to a thermal source using thermal cues, triggering the Proboscis Extension Reflex at a specific distance from the target even without physical contact. Walking freely is necessary for accurate distance estimation, and mechanical stimuli can affect the triggering of PER.
JOURNAL OF INSECT PHYSIOLOGY
(2021)
Article
Physiology
Petter F. Entringer, David Majerowicz, Katia C. Gondim
Summary: Insects, such as the kissing bug Rhodnius prolixus, rely on dietary cholesterol for cell membrane composition and hormone production. This study found that both the anterior and posterior midguts of R. prolixus can absorb cholesterol from ingested blood, with the anterior midgut absorbing more. Additionally, the fat body and ovaries of the insects also incorporate and store cholesterol, with active cholesterol transport being indicated at low temperatures.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Radouane Ouali, Larissa Rezende Vieira, Didier Salmon, Sabrina Bousbata
Summary: Understanding the immune physiology of Rhodnius prolixus, a major Chagas disease vector, through the characterization of its hemolymph proteome provides new insights into triatomine immunology. The study reveals that Trypanosoma cruzi triggers an early systemic immune response in the hemolymph, leading to the activation of various immune proteins and the overexpression of specific proteins. Additionally, novel proteins with immune properties, such as the putative c1q-like protein and the immunoglobulin I-set domain-containing protein, have been identified. The findings contribute to a better understanding of the host-parasite interaction and pave the way for the development of new strategies to control vector populations and pathogen transmission.
Article
Biochemistry & Molecular Biology
Eugenia E. Montiel, Francisco Panzera, Teresa Palomeque, Pedro Lorite, Sebastian Pita
Summary: Rhodnius prolixus' satellite DNA accounts for 8% of the total genome, composed of 39 satDNA families, including four shared with Triatoma infestans, as well as telomeric and GATA repeats. The clustering analysis revealed that most abundant satDNA families configured several superclusters, indicating complexity in R. prolixus' satellitome, with the four most abundant satDNA families being composed by different subfamilies. Transcription analysis of satDNA families in different tissues showed that 33 out of 39 families are transcribed in four different patterns of expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jose Manuel Latorre-Estivalis, Ewald Grosse-Wilde, Gabriel da Rocha Fernandes, Bill S. Hansson, Marcelo Gustavo Lorenzo
Summary: This study investigated the behavioral responses of Rhodnius prolixus to host cues and bug signals at different ages post-ecdysis, and found significant modulation of sensory-related gene expression. The study revealed substantial changes in gene expression in the antenna of R. prolixus during the initial days after molting, followed by stable expression profiles. For the first time, the maturation process of the peripheral sensory system of a hemimetabolous insect was described at the molecular level.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Microbiology
Sunyia Hussain, Janine H. Peterson, Harris D. Bernstein
Summary: The assembly of trimeric porins involves a stepwise process that requires the presence of the Bam complex and LPS. The interaction with LPS is not only essential for trimerization but also for the productive insertion of individual subunits into the lipid bilayer.
Article
Biochemistry & Molecular Biology
Laila C. Andrade, David Majerowicz, Pedro L. Oliveira, Alessandra A. Guarneri
Summary: In this study, the researchers investigated the effects of T. rangeli infection on the locomotory activity and triglyceride metabolism of Rhodnius prolixus nymphs. They found that infected nymphs had increased activity when starved and an accumulation of glycerides in the fat body and hemolymph. These alterations were associated with changes in the expression of key genes related to triglyceride metabolism in the fat body. The findings suggest that T. rangeli is able to alter the energetic processes of its invertebrate host to increase lipid availability for the parasite and modify host activity levels, potentially increasing the transmission rate of the parasite.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)
Article
Physiology
Ana Caroline P. Gandara, Felipe A. Dias, Paula C. de Lemos, Renata Stiebler, Ana Cristina S. Bombaca, Rubem Menna-Barreto, Pedro L. Oliveira
Summary: Low levels of ROS play essential roles in cell signaling, with RpNOX5 and RpXDH regulating ROS in the digestive physiology of the kissing bug Rhodnius prolixus. Silencing these genes led to early mortality in adult females, impaired gut motility, decreased egg production, and disrupted hemoglobin digestion, indicating their crucial role in blood digestion control. Crosstalk between RpNOX5 and RpXDH influences redox metabolism and nitrogen excretion, highlighting their central role in digestive physiology.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Microbiology
Tarcisio Fontenele de Brito, Vitor Lima Coelho, Maira Arruda Cardoso, Ingrid Alexandre de Abreu Brito, Mateus Antonio Berni, Fides Lea Zenk, Nicola Iovino, Attilio Pane
Summary: The study demonstrates that Rhodnius prolixus can be infected by a variety of RNA viruses from distantly related families, which are vertically transmitted from mothers to progeny. The presence of viral small interfering RNAs in eggs suggests that RNAi mechanisms play a role in reducing viral load and protecting the development of embryos. These findings significantly expand knowledge of virus complexity in triatomines and may lead to the development of novel insect population control strategies.
Article
Biochemistry & Molecular Biology
Kate K. S. Batista, Cecilia S. Vieira, Marcela B. Figueiredo, Samara G. Costa-Latge, Patricia Azambuja, Fernando A. Genta, Daniele P. Castro
Summary: Antibiotic treatment and recolonization with different bacteria affected the humoral immune response of Rhodnius prolixus, potentially playing a crucial role in the development and transmission of Trypanosoma cruzi and Trypanosoma rangeli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Fisheries
Cecilia Stahl Vieira, Marcela Barbosa Figueiredo, Caroline da Silva Moraes, Suelen Bastos Pereira, Paul Dyson, Cicero Brasileiro Mello, Daniele Pereira Castro, Patricia Azambuja
Summary: This study investigated the effects of azadirachtin on the immune system and microbiota of Rhodnius prolixus, revealing the inhibition of RpRelish and several AMP genes expression, while upregulation of RpDorsal and defC AMP genes later on; furthermore, the treatment also impacted on proliferation of commensal bacteria.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Paula Beatriz Santiago, Kaio Luis da Silva Bentes, Waldeyr Mendes Cordeiro da Silva, Yanna Reis Praca, Sebastien Charneau, Soraya Chaouch, Philippe Grellier, Marcos Antonio dos Santos Silva Ferraz, Izabela Marques Dourado Bastos, Jaime Martins de Santana, Carla Nunes de Araujo
Summary: The lack of data on miRNAs from triatomine bugs (Reduviidae: Triatominae), which are the vectors of Trypanosoma cruzi, has prompted this study on the miRNome profiles of Rhodnius prolixus triatomine. Small RNA sequencing revealed abundant expression of miRNAs, along with tRNA- and rRNA-derived fragments. A total of 52 mature miRNAs, previously reported in Ecdysozoa, were identified, including 39 that were ubiquitously expressed in the three tissues. Additionally, novel miRNAs were predicted in the gut, hemolymph, and salivary glands. The study highlights the regulatory role of miRNAs in Triatominae species.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Jimena Leyria, Ian Orchard, Angela B. Lange
Summary: In adult females of several insect species, juvenile hormones (JHs) play a role as gonadotrophic hormones, regulating egg production and successful hatching through the JHSB(3) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Felipe B. Saraiva, Michele Alves-Bezerra, David Majerowicz, Lisvane Paes-Vieira, Valdir Braz, Muriel G. M. D. Almeida, Jose Roberto Meyer-Fernandes, Katia C. Gondim
Summary: In this study, the regulation of R. prolixus ACC expression and de novo lipogenesis activity in adult females under different nutritional and metabolic conditions was investigated. The blood meal induces the utilization of diet-derived amino acids by de novo lipogenesis in the fat body, and the control of this activity does not occur at the RhoprACC gene or protein expression level. These results provide insights into the synthesis and regulation of lipids in insects, particularly in the context of blood feeding and metabolic changes.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Hideaki Ogata, Koji Nishikawa, Wolfgang Lubitz
Article
Chemistry, Physical
Jessica L. Barilone, Hideaki Ogata, Wolfgang Lubitz, Maurice van Gastel
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2015)
Article
Biochemistry & Molecular Biology
James A. Birrell, Christoph Laurich, Edward J. Reijerse, Hideaki Ogata, Wolfgang Lubitz
Review
Biochemistry & Molecular Biology
Hideaki Ogata, Wolfgang Lubitz, Yoshiki Higuchi
JOURNAL OF BIOCHEMISTRY
(2016)
Article
Chemistry, Multidisciplinary
Chunmao He, Hideaki Ogata, Wolfgang Lubitz
Article
Chemistry, Physical
Elena Decaneto, Tatiana Vasilevskaya, Yuri Kutin, Hideaki Ogata, Moran Grossman, Irit Sagi, Martina Havenith, Wolfgang Lubitz, Walter Thiel, Nicholas Cox
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2017)
Article
Chemistry, Multidisciplinary
Nipa Chongdar, James A. Birrell, Krzysztof Pawlak, Constanze Sommer, Edward J. Reijerse, Olaf Ruediger, Wolfgang Lubitz, Hideaki Ogata
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Multidisciplinary Sciences
Stefania Abbruzzetti, Alessandro Allegri, Axel Bidon-Chanal, Hideaki Ogata, Giancarlo Soavi, Giulio Cerullo, Stefano Bruno, Chiara Montali, F. Javier Luque, Cristiano Viappiani
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Min Sub Sim, Hideaki Ogata, Wolfgang Lubitz, Jess F. Adkins, Alex L. Sessions, Victoria J. Orphan, Shawn E. McGlynn
NATURE COMMUNICATIONS
(2019)
Review
Chemistry, Multidisciplinary
Koji Nishikawa, Hideaki Ogata, Yoshiki Higuchi
Article
Biochemistry & Molecular Biology
Nipa Chongdar, Krzysztof Pawlak, Olaf Ruediger, Edward J. Reijerse, Patricia Rodriguez-Macia, Wolfgang Lubitz, James A. Birrell, Hideaki Ogata
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2020)
Article
Biochemical Research Methods
Takahiro Imanishi, Koji Nishikawa, Midori Taketa, Katsuhiro Higuchi, Hulin Tai, Shun Hirota, Hironobu Hojo, Toru Kawakami, Kiriko Hataguchi, Kayoko Matsumoto, Hideaki Ogata, Yoshiki Higuchi
Summary: This study investigated the binding state of CO with the hydrogenase in Citrobacter sp. S-77 using activity assays, spectroscopic techniques, and X-ray crystallography. The results showed a positive correlation between CO tolerance and oxygen tolerance in [NiFe]-hydrogenases.
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2022)
Review
Chemistry, Multidisciplinary
Sven T. Stripp, Benjamin R. Duffus, Vincent Fourmond, Christophe Leger, Silke Leimkueshler, Shun Hirota, Yilin Hu, Andrew Jasniewski, Hideaki Ogata, Markus W. Ribbe
Summary: Gases like H-2, N-2, CO2, and CO are important feedstock for green energy conversion and as sources of nitrogen and carbon. However, their industrial transformation and production require significant energy input, whereas nature efficiently converts them at ambient conditions using gas-processing metalloenzymes (GPMs). In this review, the importance of the cofactor/protein interface in GPMs is emphasized, and the effects of second and outer coordination sphere on catalytic activity are discussed.
Article
Chemistry, Multidisciplinary
Nipa Chongdar, Patricia Rodriguez-Macia, Edward J. Reijerse, Wolfgang Lubitz, Hideaki Ogata, James A. Birrell
Summary: This study investigates the influence of the protein environment on the catalytic, spectroscopic, and redox properties of [FeFe] hydrogenases. Mutations at the non-conserved serine 267 site resulted in a significant decrease in activity and a lower redox potential for the [4Fe-4S] subcluster. These findings highlight the important role of the secondary coordination sphere in tuning the catalytic properties of [FeFe] hydrogenases.
Article
Chemistry, Multidisciplinary
Takeshi Hiromoto, Koji Nishikawa, Seiya Inoue, Hideaki Ogata, Yuta Hori, Katsuhiro Kusaka, Yu Hirano, Kazuo Kurihara, Yasuteru Shigeta, Taro Tamada, Yoshiki Higuchi
Summary: This study reports that [NiFe]-hydrogenase from Desulfovibrio vulgaris Miyazaki F can recover its catalytic activity by reacting with H2 under anaerobic conditions after being inactivated in the presence of O2. Neutron structure analysis revealed that a part of the Ni ion dissociates from the active site Ni-Fe complex and forms a new square-planar Ni complex, accompanied by rearrangement of the coordinated thiolate ligands. The analysis also found that the Cys17(S) thiolate forms an unusual hydrogen bond with the main-chain amide N atom of Gly19(S), providing insights into the redox reaction of the Fe-S cluster.
