Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 67, Issue -, Pages 692-695Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309111013297
Keywords
-
Funding
- Ministry of Education, Science, Sports and Culture of Japan [20750137, 20350078]
- Grants-in-Aid for Scientific Research [23750190, 20750137, 20350078] Funding Source: KAKEN
Ask authors/readers for more resources
The soluble region (residues 32-354) of GK0767, a copper-containing nitrite reductase from the thermophilic Gram-positive bacterium Geobacillus kaustophilus HTA426, has been cloned and overexpressed in Escherichia coli. The purified recombinant protein was crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected and processed to a maximum resolution of 1.3 angstrom. The crystals belonged to space group R3, with unit-cell parameters a = b = 115.1, c = 87.5 angstrom. Preliminary studies and molecular-replacement calculations reveal the presence of one subunit of the homotrimeric structure in the asymmetric unit; this corresponds to a V-M value of 3.14 angstrom(3) Da(-1).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available