Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 66, Issue -, Pages 418-420Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1744309110004409
Keywords
Mycobacterium tuberculosis; chaperonins; GroEL1
Funding
- National Institutes of Health [R01-AI076239, R01-GM067672]
- Department of Defense
- American Cancer Society
- Robert A. Welch Foundation [Q-1530]
- Keck Center of the Gulf Coast Consortia [R90-DK071505]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI076239] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R90DK071505] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM067672] Funding Source: NIH RePORTER
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Full-length GroEL1 from Mycobacterium tuberculosis H37Rv was cloned, overexpressed and purified. Crystals were obtained by the hanging-drop vapor-diffusion method and contained a 23 kDa GroEL1 fragment. A complete native data set was collected from a single frozen crystal that belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 75.47, b = 78.67, c = 34.89 A, alpha = beta = gamma = 90 degrees, and diffracted to 2.2 A resolution on a home X-ray source.
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