Article
Chemistry, Multidisciplinary
Fang Ba, Xiangyang Ji, Shuhui Huang, Yufei Zhang, Wan-Qiu Liu, Yifan Liu, Shengjie Ling, Jian Li
Summary: Erythritol, a natural sugar alcohol, cannot be metabolized by humans or their gut microbes. However, engineered E. coli strains can utilize erythritol as a carbon source for increased cell growth. Overexpression of certain genes, such as transaldolase and transketolase, can significantly enhance cell growth. Additionally, these engineered E. coli strains can be used as a living detector to identify erythritol-containing beverages and grow in simulated intestinal fluid supplemented with erythritol.
Article
Agriculture, Multidisciplinary
Daoyi Guo, Shaoting Wu, Xiao Fu, Hong Pan
Summary: This study established a biosynthetic pathway for the production of methyl cinnamate from glucose in engineered Escherichia coli, and successfully increased the titer of methyl cinnamate production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Biographical-Item
Biotechnology & Applied Microbiology
Gerd P. Pfeifer, Judith Singer-Sam, Keiichi Itakura
Summary: Arthur Riggs, a world-renowned diabetes expert and molecular biologist, played a pioneering role in the development of life-saving recombinant protein and antibody drugs that formed the foundation of the biotech industry.
NATURE BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Qiang Guo, Ling-Jie Zheng, Xuan Luo, Xin-Quan Gao, Chen-Yang Liu, Li Deng, Li-Hai Fan, Hui-Dong Zheng
Summary: This study proposed a new method to efficiently produce D-allulose through fermentation in Escherichia coli using metabolic engineering techniques, achieving a high yield of D-allulose.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Rie Jonsson, Alexander Bjorling, Soren Roi Midtgaard, Grethe Vestergaard Jensen, Nicholas Skar-Gislinge, Lise Arleth, Steve Matthews, Karen Angeliki Krogfelt, Havard Jenssen
Summary: This study used small-angle X-ray scattering to reveal the structural morphology of the aggregative adherence fimbriae. The results showed that the fimbriae were arranged in a compact manner with Agg5A + Agg3B subunit proteins assembled pairwise on a flexible string. The study also suggests that the minor subunit plays a significant role in the assembled fimbriae by affecting the overall structure.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Menghua Wang, Katherine S. Ryan
Summary: In this study, two new l-Alanosine-producing strains were discovered through genome mining. These strains lack the aspartate-nitrosuccinate pathway genes found in the original producer and use a unique set of nitrate-nitrite reductases to reduce nitrate instead. The repurposing of enzymes from the nitrogen cycle for specialized metabolite biosynthesis and the potential for the discovery of new nitric-oxide-derived natural products are demonstrated.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biotechnology & Applied Microbiology
Qiang Guo, Chen-Yang Liu, Ling-Jie Zheng, Shang-He Zheng, Ya-Xing Zhang, Su-Ying Zhao, Hui-Dong Zheng, Li-Hai Fan, Xiao-Cheng Lin
Summary: A metabolic pathway in Escherichia coli was designed and constructed to produce D-allulose from D-fructose via in vivo phosphorylation-dephosphorylation. Blocking the carbon flux and introducing an ATP regeneration system improved fermentation performance, resulting in high D-allulose titer and yield.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biology
Matthew D'lorio, Ken Dewar
Summary: The structural arrangements of bacterial chromosomes vary widely between closely related species and can result in significant phenotypic outcomes. This study identifies replication-associated structural rearrangements (RASRs) in bacteria and investigates their locations and potential biological mechanisms. The findings suggest that large-scale chromosomal inversions, often symmetric relative to markers for the origin of replication, can occur through repairing breaks formed during the replication process.
LIFE SCIENCE ALLIANCE
(2022)
Article
Microbiology
Taiki Chikada, Tomomi Kanai, Masafumi Hayashi, Taishi Kasai, Taku Oshima, Daisuke Shiomi
Summary: Gram-negative bacteria like Escherichia coli have an outer membrane with peptidoglycan that allows them to withstand turgor pressure. Wall-deficient cells, known as L-form cells, can grow well under anaerobic conditions and require a rigid outer membrane to survive, but not too rigid to prevent them from changing cell shape.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ilaria D'Agostino, Githa Elizabeth Mathew, Paola Angelini, Roberto Venanzoni, Giancarlo Angeles Flores, Andrea Angeli, Simone Carradori, Beatrice Marinacci, Luigi Menghini, Mohamed A. Abdelgawad, Mohammed M. Ghoneim, Bijo Mathew, Claudiu T. Supuran
Summary: The COVID-19 pandemic has highlighted the urgent need for new antimicrobials to combat cross-resistance. Thiosemicarbazones (TSCs) have emerged as a promising option due to their polypharmacological application. Further research is needed to explore their potential in addressing antimicrobial resistance.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yang Li, Ying Wang, Ruxiang Wang, Xiongying Yan, Jiewen Wang, Xia Wang, Shouwen Chen, Fengwu Bai, Qiaoning He, Shihui Yang
Summary: In this study, a heterologous poly-3-hydroxybutyrate (PHB) pathway was engineered into Zymomonas mobilis, resulting in increased PHB production. By combining genetic strategies and process engineering, the researchers were able to optimize PHB content in recombinant strains and even generate a self-flocculating strain with higher PHB content. Furthermore, Z. mobilis showed potential for continuous co-production of high-titer ethanol and PHB.
Article
Microbiology
Carl J. Stone, Gwyneth F. Boyer, Megan G. Behringer
Summary: Methylated DNA adenines (6mA) are important epigenetic modifications in bacteria, and this study reveals variations in 6mA levels across bacterial genomes. Furthermore, it shows that MMR- populations acquire more epimutations during evolution, resulting in a decrease in 6mA methylation.
Article
Microbiology
Maria Juviann Isaacs, Dineshram Ramadoss, Ashutosh Shankar Parab, Cathrine Sumathi Manohar
Summary: This study characterized the fatty acid profiles of bacterial isolates collected from the southwest coast of India, revealing specific differences in fatty acid composition between isolates from different regions. The reliability of the bacterial identification system was evaluated using 16S rRNA gene sequencing techniques.
CURRENT MICROBIOLOGY
(2021)
Article
Microbiology
Aleksandra Petrovic Fabijan, David Martinez-Martin, Carola Venturini, Katarzyna Mickiewicz, Neftali Flores-Rodriguez, Jeff Errington, Jonathan Iredell
Summary: Cell wall deficient bacterial L-forms can be induced by antibiotics and immune effectors. Pathogenic E. coli can switch to L-forms and back to a cell-walled state in the presence of meropenem antibiotics, and these L-forms are completely resistant to antibiotics targeting penicillin-binding proteins. The reversion to the cell-walled state can occur within 20 hours after antibiotic cessation, with minimal changes in DNA sequence.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
Shir Marciano, Debabrata Dey, Dina Listov, Sarel J. Fleishman, Adar Sonn-Segev, Haydyn Mertens, Florian Busch, Yongseok Kim, Sophie R. Harvey, Vicki H. Wysocki, Gideon Schreiber
Summary: In this study, the oligomeric state of 17 different bacterial proteins in E. coli was re-evaluated using various methods, and it was found that most proteins exhibit multiple oligomeric states. Some proteins did not show mass-action driven equilibrium between oligomeric states. The predicted oligomeric forms underestimated the complexity of protein quaternary structures in solution, while AlphaFold multimer provided an accurate description.
Article
Chemistry, Multidisciplinary
Katarzyna Rzesikowska, Mateusz Jablonski, Justyna Kalinowska-Tluscik
STRUCTURAL CHEMISTRY
(2020)
Article
Chemistry, Multidisciplinary
Ewa Zeslawska, Justyna Kalinowska-Tluscik, Wojciec Nitek, Henryk Marona, Anna M. Waszkielewicz
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY
(2020)
Article
Chemistry, Applied
Jingyao Li, Vincenzo Di Lorenzo, Pravin Patil, Angel J. Ruiz-Moreno, Katarzyna Kurpiewska, Justyna Kalinowska-Tluscik, Marco A. Velasco-Velazquez, Alexander Domling
ACS COMBINATORIAL SCIENCE
(2020)
Article
Chemistry, Multidisciplinary
Sylvain Aumonier, Gianluca Santoni, Guillaume Gotthard, David von Stetten, Gordon A. Leonard, Antoine Royant
Article
Biochemical Research Methods
Liyana Azmi, Eilis C. Bragginton, Ian T. Cadby, Olwyn Byron, Andrew J. Roe, Andrew L. Lovering, Mads Gabrielsen
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Syed Feroj Ahmed, Lori Buetow, Mads Gabrielsen, Sergio Lilla, Chatrin Chatrin, Gary J. Sibbet, Sara Zanivan, Danny T. Huang
Article
Multidisciplinary Sciences
Chatrin Chatrin, Mads Gabrielsen, Lori Buetow, Mark A. Nakasone, Syed F. Ahmed, David Sumpton, Gary J. Sibbet, Brian O. Smith, Danny T. Huang
Article
Chemistry, Multidisciplinary
Mateusz Reczynski, Dawid Pinkowicz, Koji Nakabayashi, Christian Nather, Jan Stanek, Marcin Koziel, Justyna Kalinowska-Tluscik, Barbara Sieklucka, Shin-ichi Ohkoshi, Beata Nowicka
Summary: The molecule chain exhibits a unique multiresponsive behavior to stimuli such as temperature, pressure, light, and humidity, leading to significant changes in magnetic susceptibility and color. This stimuli-responsive material can switch between two valence states and show thermal hysteresis at room temperature, as well as sensitivity to pressure and irradiation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Syed Feroj Ahmed, Lori Buetow, Mads Gabrielsen, Sergio Lilla, Gary J. Sibbet, David Sumpton, Sara Zanivan, Ann Hedley, William Clark, Danny T. Huang
Summary: The study explores the oncogenic mechanism of E3-inactive CBL mutants in myeloproliferative neoplasms, showing altered transcriptomes and disrupted CIN85 endosomal trafficking which amplify signaling events promoting oncogenesis. The inverse correlation between pCBL and both CIN85 and CBL in human breast cancer tissues suggests a potential for targeting the EGFR-CBL-CIN85 axis in CBL-inactivated mutant cancers.
Article
Spectroscopy
Wojciech Pietrus, Rafal Kurczab, Rafal Kafel, Ewa Machalska, Justyna Kalinowska-Tluscik, Adam Hogendorf, Marek Zylewski, Malgorzata Baranska, Andrzej J. Bojarski
Summary: The study explores the direct and indirect effect of fluorine on hydrogen bond patterns, revealing that a fluorine substituent decreases the strength and frequency of N-H···N hydrogen bonds while increasing the acidity of CH protons, enhancing weaker interactions. Furthermore, the position of fluorine in the aromatic ring strongly influences the C-F bond length and the stability of the N-H bond.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Mark A. Nakasone, Karolina A. Majorek, Mads Gabrielsen, Gary J. Sibbet, Brian O. Smith, Danny T. Huang
Summary: This study elucidates the mechanism of processive K48-linked polyUb chain formation by UBE2K, including acceptor Ub recognition and multivalent Ub-binding feature.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Chemistry, Organic
Xin Li, Qian Wang, Qiang Zheng, Katarzyna Kurpiewska, Justyna Kalinowska-Tluscik, Alexander Doemling
Summary: In this study, a series of reactions were used to synthesize isoquinolin-2(1H)-yl-acetamides and isoindolin-2-yl-acetamides with high yields. These products have potential applications in receptor chemistry. The scope and limitations of these reactions were also explored.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sylvain Aumonier, Sylvain Engilberge, Nicolas Caramello, David von Stetten, Guillaume Gotthard, Gordon A. Leonard, Christoph Mueller-Dieckmann, Antoine Royant
Summary: The development of serial crystallography has revitalized room-temperature macromolecular crystallography. This study describes the room-temperature structural study of a reaction intermediate using a synchrotron. The authors were able to monitor the relaxation process of the intermediate and discovered unexpected protein rearrangements.
Article
Chemistry, Physical
Katarzyna Rzesikowska, Justyna Kalinowska-Tluscik, Anna Krawczuk
Summary: Computational methods, especially molecular docking-based calculations, are essential in modern drug discovery. The scoring function is a useful energy-based element in docking software for evaluating ligand-protein binding. However, the current scoring functions often lead to incorrect selection of hit molecules and poor pose prediction.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Dariusz Ciez, Aleksandra Palasz, Krzysztof Koziel, Antonii Lutsan, Justyna Kalinowska-Tluscik
Summary: Titanium(iv) enolates of cyclododecanone are used as CH-acidic units in aldol additions, leading to the formation of β-hydroxy carbonyl compounds. The reactions are carried out at low temperatures to minimize dehydration and enone formation. Unlike alkali metal enolates, titanium(iv) enolates of cyclododecanone favor syn-stereoselectivity. The stereoselectivity of the aldolization reaction depends on the size and location of substituents in the aromatic ring of benzaldehydes.
NEW JOURNAL OF CHEMISTRY
(2023)