Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Chemistry, Analytical
Sakda Jampasa, Chahya Kreangkaiwal, Kurt Kalcher, Wassa Waiwinya, Teerasit Techawiwattanaboon, Nopporn Songumpai, Passorn Sueyanyongsiri, Kampol Pattanasombatsakul, Malee Techapornroong, Saovanee Benjamanukul, Orawon Chailapakul, Kanitha Patarakul, Sudkate Chaiyo
Summary: This study developed a diagnostic device for leptospiral detection that integrates near-field communication and a portable smartphone. The device offers high specificity, selectivity, simplicity, and requires minimal sample volume, making it suitable for countries with limited medical facilities and resources.
ANALYTICAL CHEMISTRY
(2022)
Article
Immunology
Se Chang Oh, Soo Min Park, Jian Hur, Eun Young Choi, Hyun Jung Jin, Yu Kyung Kim, Jong Ho Lee, Ji Young Ahn, Jae Min Lee
Summary: The study investigated the effectiveness of mPCR for early diagnosis of pertussis, revealing that most patients were diagnosed within two weeks from symptom onset and required hospitalization and antibiotic treatment.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Medicine, General & Internal
Jeong-Hwa Kim, Jae-Woon Oh, Young Lee, Jeong-Ho Yun, Seong-Ho Choi, Dong-Woon Lee
Summary: This study found that detecting 18 bacterial species in mouth-rinse samples can be used to diagnose periodontitis, with Treponema denticola, Prevotella nigrescens, and Streptococcus mitis significantly associated with severe periodontitis. Further research is needed to assess the potential of oral-rinsing solutions in indicating oral infection risk and improving oral hygiene.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Analytical
Kunlun Jiang, Jingrui Wu, Yuwei Qiu, Yun Young Go, Kiwon Ban, Hyun June Park, Jung-Hoon Lee
Summary: The PPT-cPCR method is a fast, sensitive, inexpensive, and simple nucleic acid detection technique that allows for visual detection of DNA targets and easy access to quantitative information using a spectrophotometer and a smartphone.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Public, Environmental & Occupational Health
Shweta Joshi, Keerti K. Dixit, Vanila Sharma, V Ramesh, Ruchi Singh, Poonam Salotra
Summary: A multiplex loop-mediated isothermal amplification (m-LAMP) assay was developed for rapid and accurate differential detection of Mycobacterium leprae and Leishmania donovani in co-endemic areas. The diagnostic sensitivity of the m-LAMP assay was 100% for detecting PKDL and 95% for leprosy, making it a valuable tool for differential diagnosis in endemic regions.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Biotechnology & Applied Microbiology
Nicola C. Elviss, David J. Allen, Daniel Kelly, Joyce Odeke Akello, Sarah Hau, Andrew J. Fox, Mark Hopkins, Jade Derrick, Sarah O'Brien, Miren Iturriza-Gomara
Summary: This study aims to investigate whether there is a higher contamination of norovirus in the commercial food production environment in premises that have recently reported a norovirus outbreak compared to those that have not. The results demonstrate that food premises and food handlers remain a potential source of norovirus transmission and outbreaks.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Linlin Zhuang, Jianbo Yang, Chunlei Song, Li Sun, Bin Zhao, Qiuping Shen, Xiyan Ren, Hongjing Shi, Yu Zhang, Mengling Zhu
Summary: An accurate and practical graphene oxide-based method, called GO-ASEA, was developed for rapid and highly sensitive detection of African swine fever virus (ASFV). This novel assay showed excellent specificity and selectivity, with a low detection limit, and demonstrated good clinical applicability. The GO-ASEA method has the potential to serve as a robust tool in epidemic prevention and point-of-care diagnosis.
ANALYTICAL METHODS
(2022)
Article
Medicine, General & Internal
Tobias Todsen, Kathrine K. Jakobsen, Mathias Peter Gronlund, Rasmus E. Callesen, Fredrik Folke, Helene Larsen, Annette Kjaer Ersboll, Thomas Benfield, Tobias Gredal, Mads Klokker, Nikolai Kirkby, Christian von Buchwald
Summary: This study compared the diagnostic accuracy of self- and HCW-collected nasal and throat swab specimens for COVID-19 rapid antigen testing. The study found that HCW-collected throat specimens had higher sensitivity than HCW-collected nasal specimens for rapid antigen testing. However, among symptomatic participants, self-collected nasal specimens had higher sensitivity than self-collected throat specimens.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Veterinary Sciences
Juan Jose Badiola, Alicia Otero, Eloisa Sevilla, Belen Marin, Mirta Garcia Martinez, Marina Betancor, Diego Sola, Sonia Perez Lazaro, Jenny Lozada, Carolina Velez, Alvaro Chiner-Oms, Inaki Comas, Irving Cancino-Munoz, Eva Monleon, Marta Monzon, Cristina Acin, Rosa Bolea, Bernardino Moreno
Summary: This study reports the outbreak of SARS-CoV-2 on a mink farm in Spain. The minks did not show clinical signs of infection, but a high percentage of them were positive for the virus. It was also found that minks could contribute to the spread of SARS-CoV-2 among animals and humans. Continuous surveillance of mink farms is therefore important.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemical Research Methods
Jinrong Shen, Jihong Zheng, Zhenqing Li, Yourong Liu, Fengxiang Jing, Xinjun Wan, Yoshinori Yamaguchi, Songlin Zhuang
Summary: The study introduces an effective technique for the absolute quantification of target nucleic acids, with self-developed software controlling functional units to achieve high-resolution one-time gene chip imaging, speeding up the lab-to-application process.
Article
Chemistry, Analytical
Bobin Lee, Yeonghun Lee, Seung-Mo Kim, Kihyeun Kim, Min-Gon Kim
Summary: The incidence of deadly infectious diseases has been increasing worldwide in recent years, leading to a high demand for rapid and accurate nucleic acid diagnostic systems. In this study, a rapid membrane-based photothermal polymerase chain reaction (PCR) system was developed, allowing for quantitative fluorescence detection. This system overcomes the limitations of conventional PCR and offers user-friendly micro-scale solution control and sample loading. The reaction pad is coated with an Au nanostructure that acts as a light-to-heat converter for thermocycling. The reaction solution is heated using a 785 nm light source, and the temperature is cycled 25 times between 63°C and 95°C within 6 minutes. The amplified samples on the membrane are then quantitatively analyzed by measuring the fluorescence signal. This membrane-based PCR approach can be easily integrated with various sample pre-treatment processes, making it a promising all-in-one molecular diagnosis platform.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Editorial Material
Urology & Nephrology
Rakesh Kumar Pilania, Deepanjan Bhattacharya, Neelam Taneja, Amit Rawat, Deepti Suri, Raja Ramachandran, Karalanglin Tiewsoh
JOURNAL OF NEPHROLOGY
(2021)
Article
Immunology
Megan E. Carey, Ruby Jain, Mohammad Yousuf, Mailis Maes, Zoe A. Dyson, Trang Nguyen Hoang Thu, To Nguyen Thi Nguyen, Thanh Ho Ngoc Dan, Quynh Nhu Pham Nguyen, Jaspreet Mahindroo, Duy Thanh Pham, Kawaljeet Singh Sandha, Stephen Baker, Neelam Taneja
Summary: The study in Northern India identified 66 S. Typhi organisms with antimicrobial resistance, including 7 strains with R717Q mutation in the acrB gene conferring resistance to azithromycin and 6 strains with triple mutations in gyrA and parC genes leading to ciprofloxacin resistance. This findings suggest an urgent need for typhoid conjugate vaccines introduction in South Asia as an emerging broader problem of antimicrobial resistance.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Pediatrics
Vinay Modgil, Pankaj Chaudhary, Bhavneet Bharti, Jaspreet Mahindroo, Md Yousuf, Meenakshi Koundal, Balvinder Mohan, Neelam Taneja
Summary: The study aimed to investigate the role of enteroaggregative Escherichia coli (EAEC) in childhood diarrhea and as a commensal in children younger than five years old. EAEC detection was higher in children with acute diarrhea compared to nourished and malnourished controls. Adhesive variants like adhesion fimbriae IV and II were significantly associated with diarrhea, and aggR and aar genes showed associations with disease severity. High rates of multidrug resistance were observed in the diarrheal group. Phylogenetic analysis revealed differences in EAEC strains between diarrheal and non-diarrheal groups.
JOURNAL OF PEDIATRICS
(2021)
Article
Immunology
Harpreet Kaur, Manmohit Kalia, Neelam Taneja
Summary: The study identified novel drug targets for the treatment of infections caused by multidrug-resistant Acinetobacter baumannii through three phases of analysis, and discovered some new drug targets.
MICROBIAL PATHOGENESIS
(2021)
Article
Immunology
Vinaykumar Hallur, Sutapa Rath, Mukund Sable, Punyatoya Kar, Shivaprakash M. Rudramurthy, Tushar Mishra, Vaishnavi Deshmukh
Summary: This article reports a rare case of phaeohyphomycosis caused by Phaeoacremonium krajdenii. The patient is a healthy woodcutter who presented with a cystic swelling on the sole of the left great toe. The lesion was successfully cured after aspiration.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Immunology
Naveen Chaudhary, Chandradeo Narayan, Balvinder Mohan, Neelam Taneja
Summary: The study characterized a phage RDN37 isolated from community sewage water in Chandigarh, which showed activity against multidrug-resistant Uropathogenic E. coli. The phage demonstrated stability, lytic activity against resistant strains, and potential therapeutic application for treating drug-resistant urinary tract infections.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Immunology
Geetarani Purohit, Mukund Sable, Shivaprakash M. Rudramurthy, Saurav Sarkar, Pradipta Parida, Vaishnavi Deshmukh, Vinaykumar Hallur
Summary: This report presents the first case of atypical conidiobolomycosis from Odisha, India, characterized by dysphagia and significant weight loss. The diagnosis was made through histopathology and fungal culture, and the patient was successfully treated with saturated solution of potassium iodide.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Immunology
Neelam Taneja, Jutang Babat Ain Tiewsoh, Swati Gupta, Balvinder Mohan, Ritu Verma, Pinky Shankar, Chandradeo Narayan, Vivek Kumar Yadav, Muralidharan Jayashree, Surjit Singh
Summary: The study conducted at a tertiary care center in North India found that the most common serogroup of Shigella isolated was Shigella flexneri, with increasing drug resistance observed. Children under 5 years old had the highest incidence rate, while males were also significantly affected.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Microbiology
Vinaykumar Hallur, Hariprasath Prakash, Mukund Sable, Chappity Preetam, Prashanth Purushotham, Rabindra Senapati, Shamanth Adekhandi Shankarnarayan, Nerbadyswari Deep Bag, Shivaprakash Mandya Rudramurthy
Summary: This study describes a new species of Cunninghamella causing chronic rhino-orbital-cerebral disease and reviews cases of mucormycosis due to Cunninghamella spp. in immunocompetent individuals. Despite repeated treatment attempts, the patient experienced multiple recurrences with intracranial extension and eventually succumbed to the illness.
Article
Microbiology
Naveen Chaudhary, Dharminder Singh, Chandradeo Narayan, Bhaskar Samui, Balvinder Mohan, Ravimohan S. Mavuduru, Neelam Taneja
Summary: Escherichia phage 590B, isolated from community sewage water in Chandigarh, India, shows lytic activity against extensively drug-resistant uropathogenic Escherichia coli. The phage has a linear, double-stranded genome of 44.39 kb and belongs to the Siphoviridae family, with closest similarity to phage vB_EcoS_XY2 isolated in China.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biochemistry & Molecular Biology
Harpreet Kaur, Vikram Singh, Manmohit Kalia, Balvinder Mohan, Neelam Taneja
Summary: Urinary tract infections are a serious health concern worldwide, especially in developing countries. The highly virulent Escherichia coli strain CFT073 contains 992 hypothetical proteins. Computational tools were used to analyze and classify these proteins, revealing their roles in various functional categories and metabolic pathways. Further analysis identified 35 non-homologous proteins as potential drug design targets, with 8 proteins showing virulence factors and 6 proteins successfully modelled for three-dimensional structures. The study provides valuable insights for understanding disease mechanisms and developing new drug targets against CFT073.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Editorial Material
Microbiology
Jai Ranjan, Vinaykumar Hallur
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Editorial Material
Microbiology
Jai Ranjan, Vinaykumar Hallura
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Immunology
Vinaykumar Hallur, Rumita Dey, Mukund Sable, Shivaprakash M. Rudramurthy, Anupam Dey
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Editorial Material
Public, Environmental & Occupational Health
Neelam Taneja, Swati Gupta, Jutang Babat Ain Tiewsoh, Sonakshi Srivastava
Summary: Cholera, a diarrheal disease causing major health issues in developing countries, is most prevalent in India, where vaccination and improved sanitation are key in controlling outbreaks.
INDIAN JOURNAL OF PUBLIC HEALTH
(2021)
Review
Immunology
G. Vithiya, P. Shunmuga Sundaram
Summary: This case describes a patient who developed infection after percutaneous transluminal coronary angioplasty and subsequently died due to improper treatment, primarily caused by endocarditis caused by M. abscessus.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2024)
Article
Immunology
Pheba Stanley, Nonika Rajkumari, Monika Sivaradjy
Summary: The study aimed to detect the mutation patterns in drug targets in Plasmodium vivax that develop resistance to common antimalarial agents in India. By analyzing 27 Plasmodium vivax isolates, the study found an increase in the proportion of double mutants in pvmdr1 and pvdhfr over time. Cases with double mutant pvmdr1 gene had a prolonged hospital stay compared to those without, indicating reduced clinical response to chloroquine.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2024)
Letter
Immunology
Amnuay Kleebayoon, Viroj Wiwanitkit
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2024)
