Article
Biotechnology & Applied Microbiology
Lei Yu, Yi Liu, Jianxin Wu, Shuang Wang, Jiangming Yu, Weiheng Wang, Xiaojian Ye
Summary: Intervertebral disc degeneration (IDD) is the pathological basis of disc degenerative diseases (DDD), characterized by reduction in the number of cells and degeneration of the extracellular matrix in the nucleus pulposus. An injectable ECM hydrogel combined with stem cell transplantation shows promise, but lacks sufficient biomechanics and stability. A genipin cross-linked decellularized nucleus pulposus hydrogel (GDH) was developed as a delivery system, showing good mechanical properties, biocompatibility, and differentiation induction in vitro. In vivo studies demonstrated that GDH loaded with adipose-derived mesenchymal stem cells (GDHA) improved stem cell survival and intervertebral disc regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Xiaopeng Zhou, Ning Shen, Yiqing Tao, Jingkai Wang, Kaishun Xia, Liwei Ying, Yuang Zhang, Xianpeng Huang, Jianming Hua, Chengzhen Liang, Qixin Chen, Fangcai Li
Summary: In this study, nucleus pulposus cells (NPCs) were aggregated into pellets and NPC-derived efficient microcarriers (NPCMs) were fabricated by optimized decellularization. The injectable NPCMs exhibited good biostability and no cytotoxicity. In vitro experiments showed that the NPCMs promoted the NP-specific differentiation of adipose-derived stem cells (ADSCs). In vivo experiments in a rabbit model demonstrated that ADSC-loaded NPCMs partly restored degenerated nucleus pulposus.
Article
Biotechnology & Applied Microbiology
Weiya Tang, Jun Qi, Qian Wang, Yaping Qu, Su Fu, Jie Luan
Summary: This study investigated the effects of tissue origins on the adipogenic capacity of decellularized adipose-derived matrix (DAM) by comparing it with acellular dermal matrix (ADM). The results showed that DAM could induce adipogenic differentiation and promote neovascularization and tissue remodeling in vivo. Therefore, DAM has important applications in soft tissue repair and regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Lu Cui, Yujia Zhao, Yuxuan Zhong, Lanlan Zhang, Xinnan Zhang, Zhenglong Guo, Fanglin Wang, Xin Chen, Hao Tong, Jun Fan
Summary: Adipose tissue engineering using composite hydrogels of DAT, SIS, and Adv shows promising results in promoting angiogenesis and adipogenesis, offering potential clinical applications in soft tissue regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Constanze Kuhlmann, Thilo L. Schenck, Attila Aszodi, Riccardo E. Giunta, Paul Severin Wiggenhauser
Summary: This study investigated the influence of different zones in human nasal septal cartilage on the fate of adipose tissue-derived stem cells. The findings suggest that the zonal-dependent architecture and composition of nasal cartilage modulate the activities of seeded stem cells, providing new insights for engineering cartilage substitutes in facial reconstructive surgery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Yu Zhang, Yifeng Li, Chen Liu, Xifu Shang
Summary: Three DNPM/chitosan hybrid hydrogels were fabricated and the best one was chosen for NP tissue engineering through evaluation of NP stem cells. In vivo experiment showed that DNPM/chitosan hybrid hydrogel-1 exhibited good biocompatibility.
GLOBAL SPINE JOURNAL
(2022)
Article
Anatomy & Morphology
Aida Nahumi, Maryam Peymani, Asadollah Asadi, Arash Abdolmaleki, Yassin Panahi
Summary: Tissue engineering research has focused on the characteristics and biocompatibility of decellularized sheep tracheal scaffolds, as well as the differentiation of Adipose-derived stem cells (AD-MSCs) into tracheal cells. The prepared scaffolds show potential for engineering applications and provide a suitable environment for stem cell differentiation. This research serves as a foundation for future studies on tracheal decellularization scaffolds and paves the way for their use in organ regeneration and clinical medicine.
Article
Biochemistry & Molecular Biology
Silvia Barbon, Elena Stocco, Martina Contran, Federico Facchin, Rafael Boscolo-Berto, Silvia Todros, Deborah Sandrin, Filippo Romanato, Piero Pavan, Veronica Macchi, Vincenzo Vindigni, Franco Bassetto, Raffaele De Caro, Andrea Porzionato
Summary: This study considers the use of cellular human diaphragm as a scaffold material for the treatment of volumetric muscle loss (VML). After decellularization through four detergent-enzymatic protocols, cells, DNA, and muscle fibers were effectively removed, while collagen/elastin and the majority of the glycosaminoglycan component were preserved. Adipose-derived stem cells cultured with decellularized samples demonstrated high viability, indicating the biosafety of the scaffolds. Acellular diaphragmatic grafts treated with SDS+Tergitol (TM) showed better collagen preservation and integrated well with host tissue upon implantation in mice.
Article
Biochemistry & Molecular Biology
Flavia Carton, Dalila Di Francesco, Luca Fusaro, Emma Zanella, Claudio Apostolo, Francesca Oltolina, Diego Cotella, Maria Prat, Francesca Boccafoschi
Summary: The study evaluated the myogenic potential of dECM substrate derived from decellularized bovine pericardium on murine muscle cells and found that dECM could support and enhance the myogenic potential of muscle cells compared to cells grown on a standard plastic surface. This suggests that extracellular matrix proteins could be a promising therapeutic strategy for skeletal muscle tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Guillaume Rougier, Louis Maistriaux, Lies Fieve, Daela Xhema, Robin Evrard, Julie Manon, Raphael Olszewski, Fabien Szmytka, Nicolas Thurieau, Jean Boisson, Natacha Kadlub, Pierre Gianello, Catherine Behets, Benoit Lengele
Summary: This paper investigates the possibility of creating a living bone allograft using the perfusion/decellularization/recellularization (PDR) technique. The results show that the decellularized vascularized bone grafts successfully preserve the 3D morphology and extracellular matrix (ECM) microarchitecture, promote cell growth and differentiation, and offer new perspectives for repairing large bone defects and bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Jana C. Blum, Thilo L. Schenck, Alexandra Birt, Riccardo E. Giunta, Paul S. Wiggenhauser
Summary: In this study, the combination of cell-derived artificial extracellular matrix with 3D printed polycaprolactone scaffolds showed promising results in improving bioactivity and differentiation of human adipose derived stem cells. The ac/aoPCL scaffolds demonstrated good biocompatibility and enhanced chondrogenic and osteogenic differentiation potential.
JOURNAL OF TISSUE ENGINEERING
(2021)
Article
Cell Biology
Silvia Barbon, Andrea Biccari, Elena Stocco, Giovanni Capovilla, Edoardo D'Angelo, Martina Todesco, Deborah Sandrin, Andrea Bagno, Filippo Romanato, Veronica Macchi, Raffaele De Caro, Marco Agostini, Stefano Merigliano, Michele Valmasoni, Andrea Porzionato
Summary: Esophageal reconstruction using bio-engineered allografts that resemble the tissue extracellular matrix (ECM) properties is a promising strategy. This study successfully decellularized human esophagus using three detergent-enzymatic protocols and demonstrated preservation of ECM components, retention of collagen and elastin, and reduced immunogenicity. It also showed changes in tissue stiffness but maintained sample integrity. The study highlights the potential of using decellularized esophageal matrices for future tissue repopulation and orthotopic implantation.
Article
Chemistry, Physical
Jing Ren, Jinghan Chi, Baishi Wang, Lingli Guo, Yudi Han, Xinhui Liu, Xuetao Pei, Wen Yue, Yan Han
Summary: This study demonstrated that the combination of human adipose-derived stem cells (hASCs) and a human decellularized adipose tissue matrix (hDAM) could accelerate wound healing and promote angiogenesis, showing potential for clinical application.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Engineering, Biomedical
Cheng-En Chiang, Yi-Qiao Fang, Chao-Ting Ho, Marisa Assuncao, Sheng-Ju Lin, Yu-Chieh Wang, Anna Blocki, Chieh-Cheng Huang
Summary: The study developed 3D decellularized ECM scaffolds from MSC spheroids, which preserved structural ECM components and growth factors, resulting in enhanced proangiogenic activity. These scaffolds could be populated by endothelial cells to build larger cell-carrying constructs, enhancing intrinsic revascularization in vivo.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Marco A. Herrera Quijano, Nadia Sharma, Pascal Morissette Martin, Cheryle A. Seguin, Lauren E. Flynn
Summary: This study developed and characterized a cell culture platform incorporating decellularized nucleus pulposus (DNP) for tissue regeneration and cell differentiation. The results showed that the 2-D coatings derived from DNP could support cell attachment and growth, but did not maintain or rescue the phenotype of primary cells. Similarly, the incorporation of DNP particles within the 3-D hydrogels was insufficient to maintain or rescue changes in cell phenotype associated with extended in vitro culture.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)