Review
Biotechnology & Applied Microbiology
Jose Carlos Rodriguez-Cabello, Israel Gonzalez De Torre, Miguel Gonzalez-Perez, Fernando Gonzalez-Perez, Irene Montequi
Summary: The cutting-edge strategies in biomaterials science are focused on mimicking the design of natural systems, particularly the structure of the extracellular matrix (ECM), while incorporating biomechanical and biomolecular cues for biomedical applications. Nanofiber-based scaffolds are actively studied for tissue engineering and regenerative medicine, with a focus on incorporating dynamics, biochemistry and structural features of the native ECM.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Alex Bryan, Ethan Wales, Samarth Vedante, Andreu Blanquer, Dipesh Neupane, Sanjay Mishra, Lucie Bacakova, Tomoko Fujiwara, Jessica Amber Jennings, Joel D. Bumgardner
Summary: This study successfully incorporated elastin and magnesium-phosphate particles into electrospun chitosan membranes, improving their properties for burn wound applications. Evaluation of fiber size, structure, hydrophilicity, elastin and MgP incorporation, release profiles, mechanical properties, degradation, and cytocompatibility showed that the addition of elastin and MgP enhanced the performance of the membranes.
Review
Biochemistry & Molecular Biology
Fengbo Sun, Xiaodan Sun, Hetong Wang, Chunxu Li, Yu Zhao, Jingjing Tian, Yuanhua Lin
Summary: This review systematically summarizes the research progress of 3D-printed, PLGA-based scaffolds, introduces the properties of the modified components, analyzes the influence of structure and printing method change in the printing process, illustrates the advantages and disadvantages of their applications with examples, and finally discusses the limitations and future development direction of current 3D-printed, PLGA-based materials for bone tissue repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Noah Z. Laird, Timothy M. Acri, Jaidev L. Chakka, Juliana C. Quarterman, Walla Malkawi, Satheesh Elangovan, Aliasger K. Salem
Summary: Tissue engineering is a interdisciplinary field that aims to create therapies that regenerate functional tissue by combining life sciences and engineering. The use of biologically active materials in scaffolds can enhance regeneration by enabling cell-scaffold interactions or releasing factors that aid in regeneration. Advanced techniques like nanotechnology in combination with 3D printing show promise in enhancing tissue engineering scaffolds for stimulating regeneration and cell interaction.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Review
Polymer Science
Vinna K. K. Sugiaman, Jeffrey, Silvia Naliani, Natallia Pranata, Rudy Djuanda, Rosalina Intan Saputri
Summary: The challenge in dentistry is to revitalize dental pulp using tissue engineering technology, which requires a biomaterial scaffold. The scaffold acts as a 3D framework that supports cell growth and provides a suitable environment for cell activation and communication. Selecting a safe, biocompatible scaffold with adequate characteristics is crucial in regenerative endodontics. Natural or synthetic polymer scaffolds have gained attention due to their excellent mechanical properties and good biological characteristics for cell regeneration. This review discusses the latest developments in utilizing these scaffold polymers for dental pulp tissue regeneration.
Review
Engineering, Multidisciplinary
Shike Zhang, Xin Chen, Mengyao Shan, Zijuan Hao, Xiaoyang Zhang, Lingxian Meng, Zhen Zhai, Linlin Zhang, Xuying Liu, Xianghong Wang
Summary: Three-dimensional (3D) bioprinting has great potential as a scaffold fabrication strategy for tissue engineering, with precise control over scaffold geometry and microstructure. Nanobiomaterials as bioinks play a crucial role in manipulating the cellular microenvironment to influence growth and development. This review introduces commonly used nanomaterials in tissue engineering scaffolds and discusses their impact on scaffold performance. It also comprehensively outlines different 3D bioprinting technologies and their advantages and mechanisms, and explores the application of 3D bioprinting and nanotechnology in various tissue engineering scaffolds. Furthermore, the challenges and future perspectives of the convergence of 3D bioprinting and nanotechnology are proposed. This review provides scientific guidance for developing 3D bioprinting tissue engineering scaffolds using nanotechnology.
Review
Pharmacology & Pharmacy
Francisca Villanueva-Flores, Igor Garcia-Atutxa, Arturo Santos, Juan Armendariz-Borunda
Summary: Neural tissue engineering is a promising technological breakthrough in restoring brain function. However, the development of implantable scaffolds for neural culture that meet all necessary criteria is a remarkable challenge for material science.
Article
Biochemistry & Molecular Biology
Fernando Bento Cunha, Karina Torres Pomini, Ana Maria de Guzzi Plepis, Virginia da Conceicao Amaro Martins, Eduardo Gomes Machado, Renato de Moraes, Marcelo de Azevedo e Souza Munhoz, Michela Vanessa Ribeiro Machado, Marco Antonio Hungaro Duarte, Murilo Priori Alcalde, Daniela Vieira Buchaim, Rogerio Leone Buchaim, Victor Augusto Ramos Fernandes, Eliana de Souza Bastos Mazuqueli Pereira, Andre Antonio Pelegrine, Marcelo Rodrigues da Cunha
Summary: The study investigated the effects of elastin and collagen matrix on bone repair in critical size defects in rat calvaria. The results showed that using these matrices as scaffolds was feasible and increased the formation of new bone tissue in critical size defects.
Review
Polymer Science
Jingzhi Fan, Keyvan Abedi-Dorcheh, Asma Sadat Vaziri, Fereshteh Kazemi-Aghdam, Saeed Rafieyan, Masoume Sohrabinejad, Mina Ghorbani, Fatemeh Rastegar Adib, Zahra Ghasemi, Kristaps Klavins, Vahid Jahed
Summary: The musculoskeletal system is crucial for movement, organ protection, support, hematopoiesis, and postural stability. Damage to or loss of musculoskeletal tissues greatly affects quality of life. Natural polymers have shown great potential in tissue engineering for musculoskeletal repair.
Article
Polymer Science
Denisa-Maria Radulescu, Ionela Andreea Neacsu, Alexandru-Mihai Grumezescu, Ecaterina Andronescu
Summary: In recent years, the development and characterization of biomaterials for regenerative medicine and controlled release have posed significant challenges. Hydrogels are considered optimal applications in the biomedical field, particularly in tissue engineering. However, their delicate nature and low mechanical strength limit their use. Therefore, developing stronger and more stable hydrogels with increased biocompatibility is essential. Natural and synthetic polymers both have limitations, but the development of hydrogels based on mixtures of these materials can optimize their properties. Incorporating different nanoparticles can improve stability and yield various biological effects. Essential oils and drug molecules can facilitate desired biological effects and even produce a synergistic effect. The main purpose of this study is to identify the key properties required to develop sustainable polymeric scaffolds for tissue engineering without adverse environmental effects.
Article
Polymer Science
Laura Rojas-Rojas, Maria Laura Espinoza-Alvarez, Silvia Castro-Piedra, Andrea Ulloa-Fernandez, Walter Vargas-Segura, Teodolito Guillen-Giron
Summary: Tissue engineering aims to develop in-vitro substitutes of native tissues, and one approach is using bioreactors combined with biomimetic scaffolds. This study demonstrated that a collagen-coated microfilament scaffold seeded with C2C12 myoblasts inside a custom-built bioreactor provided a promising structure for the study of skeletal muscle models. The findings have important implications for mechanical stimulation studies in tissue engineering applications.
Article
Chemistry, Multidisciplinary
Navid Toosi Saidy, Alicia Fernandez-Colino, Behzad Shiroud Heidari, Ross Kent, Michael Vernon, Onur Bas, Shane Mulderrig, Andreas Lubig, Jose Carlos Rodriguez-Cabello, Barry Doyle, Dietmar W. Hutmacher, Elena M. De-Juan-Pardo, Petra Mela
Summary: Heart valve tissue engineering aims to provide autologous heart valve implants with regenerative capabilities and durability. This study demonstrates the additive manufacturing of bioinspired tubular scaffolds using melt electrowriting, which can meet the mechanical requirements of heart valves and enable cellular infiltration. The scaffolds are then embedded with a microporous hydrogel to enhance cell infiltration and ensure compatibility with blood flow.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Thiago Domingues Stocco, Mayara Cristina Moreira Silva, Marcus Alexandre Finzi Corat, Gabriely Goncalves Lima, Anderson Oliveira Lobo
Summary: This study presents a new biofabrication strategy for developing biomimetic meniscus scaffolds. The use of bioprinted cell-laden hydrogel reinforced by aligned nanofiber sheets improved the structural integrity, shape fidelity, and mechanical properties of the scaffolds. Importantly, the introduction of nanofiber mats did not negatively affect cell viability. This bioengineering approach offers a promising strategy for tissue engineering of the knee meniscus.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Polymer Science
Stanko Kramer, Neil R. Cameron, Peter Krajnc
Summary: This review highlights the importance and versatility of high internal phase emulsions (HIPEs) and the resulting porous polymers (polyHIPEs) in biomedical applications. The focus is on the use of polyHIPEs as scaffolds for biological cell growth and tissue regeneration, along with their preparation methods and interactions with biological systems. Additionally, it touches upon the use of combined techniques and advanced applications in this field.
Article
Materials Science, Paper & Wood
A. A. Cienfuegos-Sarmiento, M. A. Martinez-Rodriguez, M. A. de la Garza-ramos, A. F. Garcia-Loera, M. A. Garza-Navarro
Summary: This study evaluated nanofibrous membranes based on polymeric blends of carboxymethyl-cellulose/silver-nanoparticle (CMC-AgNP) composite and polyvinyl alcohol (PVA) as suitable scaffolds for tissue engineering applications. The mean diameter of the nanofibers decreased as the weight content of CMC-AgNP in the polymeric blends increased. The interaction between CMC and PVA molecules, as well as the variation in nanofiber diameter, modified the mechanical properties of the membranes and prevented bacterial proliferation.
Article
Genetics & Heredity
David W. Barrett, Babatunde O. Okesola, Eleni Costa, Christopher Thrasivoulou, David L. Becker, Alvaro Mata, Jan A. Deprest, Anna L. David, Tina T. Chowdhury
Summary: Peptide amphiphiles functionalized with adhesive, migratory or regenerative sequences combined with amniotic fluid to repair fetal membrane defects. Co-culture with Cx43 antisense increased collagen levels but reduced GAG content.
PRENATAL DIAGNOSIS
(2021)
Article
Engineering, Biomedical
Adrian Lopez-Canosa, Soledad Perez-Amodio, Eduardo Yanac-Huertas, Jesus Ordono, Romen Rodriguez-Trujillo, Josep Samitier, Oscar Castano, Elisabeth Engel
Summary: The creation of cardiac tissue models for preclinical testing remains a challenge due to the limitations in replicating the complexity of cardiac tissue in vitro. In this study, a microfluidic platform was developed to provide signaling cues to immature cardiac cells, guiding them towards an adult phenotype. The platform demonstrated control over the anisotropy of cardiac tissue and induced the upregulation of key genes through electrical stimulation, validating its potential as a powerful tool for tissue engineering.
Review
Biochemistry & Molecular Biology
Diana Juanes-Gusano, Mercedes Santos, Virginia Reboto, Matilde Alonso, Jose Carlos Rodriguez-Cabello
Summary: Intrinsically disordered proteins (IDPs) lack cooperatively folded structures under native conditions, but their hybrid nature allows for flexibility and transitions between disordered and ordered states. Protein-based polymers like elastin-like polypeptides (ELPs) mimic IDPs and offer diverse applications.
JOURNAL OF PEPTIDE SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Moumita Ghosh, Anna Majkowska, Rajkumar Mirsa, Santu Bera, Jose Carlos Rodriguez-Cabello, Alvaro Mata, Lihi Adler-Abramovich
Summary: This study demonstrates the temperature-independent formation of stable membranes by the spontaneous interaction of intrinsically disordered elastin-like polypeptides (ELPs) with short aromatic peptides, offering novel possibilities for controlling the conformational restriction of intrinsically disordered proteins and using them in the design of new materials. The membranes are stable over time and display durability over a wide range of environmental parameters, supporting preosteoblast cell adhesion and proliferation as well as pH-dependent cargo release. Simple noncovalent interactions with short aromatic peptides can overcome conformational restrictions due to the phase transition to facilitate the formation of complex bioactive scaffolds.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemical Research Methods
Carlos Redondo-Gomez, Soraya Padilla-Lopategui, Alvaro Mata, Helena S. Azevedo
Summary: The host-guest-mediated PA hydrogelation method relies on the formation of a host-guest homoternary complex with CB[8] and aromatic amino-acid-bearing PA nanofibers, resulting in hierarchical morphologies and increased stiffness compared to ion-based equivalents. This alternative cross-linking method can upgrade the design of PA materials and extend their biomedical applications, while maintaining similar biocompatibilities between the new and conventional hydrogels.
BIOCONJUGATE CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Marina Paulini, Ivan Nadir Camal Ruggieri, Melina Ramallo, Matilde Alonso, Jose Carlos Rodriguez-Cabello, Pedro Esbrit, Joao Paulo Mardegan Issa, Sara Feldman
Summary: The increase in fracture rates and missing bones poses significant socio-health problems. Tissue engineering, particularly the development of scaffolds from recombinant proteins, aims to promote tissue repair and restoration. This review provides a synthetic description of the latest advances in this field, highlighting the recombinant proteins that can be used to construct scaffolds for bone regenerative medicine.
Review
Materials Science, Biomaterials
Wenhuan Bu, Yuanhao Wu, Amir M. Ghaemmaghami, Hongchen Sun, Alvaro Mata
Summary: This article summarizes how the physical and chemical properties of hydrogels affect the immune response and discusses recent advances in immunomodulatory strategies. By explaining in detail the impact of hydrogels on immune response, this review aims to provide a theoretical and practical toolkit for designing immune-instructive hydrogels capable of modulating the host immune response.
REGENERATIVE BIOMATERIALS
(2022)
Review
Biochemical Research Methods
Jordan Hill, Ricky Wildman, Alvaro Mata
Summary: The field of biofabrication has the potential to advance with the incorporation of biological assembling mechanisms into fabrication techniques. Biological organization principles such as self-assembly, compartmentalization, diffusion-reaction, disorder-to-order transitions, and out-of-equilibrium processes play a crucial role in tissue and organ formation. These approaches have shown promise in improving scalability, reproducibility, vascularization, and biomimicry in biofabrication.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yuanhao Wu, Junyao Yang, Alexander van Teijlingen, Alice Berardo, Ilaria Corridori, Jingyu Feng, Jing Xu, Maria-Magdalena Titirici, Jose Carlos Rodriguez-Cabello, Nicola M. Pugno, Jiaming Sun, Wen Wang, Tell Tuttle, Alvaro Mata
Summary: A disinfectant-assisted low temperature method to reduce GO-protein materials and fabricate surgical dressings with tuneable photothermal efficiency and bioactive properties for the postoperative treatment of melanoma is reported. In vitro and in vivo validation revealed the capacity of the dressings to prevent tumor recurrence and promote healing after tumor resection.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Abshar Hasan, Romain Bagnol, Robert Owen, Arsalan Latif, Hassan M. Rostam, Sherif Elsharkawy, Felicity R. A. J. Rose, Jose Carlos Rodriguez-Cabello, Amir M. Ghaemmaghami, David Eglin, Alvaro Mata
Summary: This study reports the development of acellular mineralizing 3D printed scaffolds for hard tissue regeneration using a combination of additive manufacturing and supramolecular chemistry. The pre-mineralized scaffolds showed improved bone ingrowth and tissue-implant integration in an in vivo model.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Arturo Ibanez-Fonseca, Ana Rico, Silvia Preciado, Fernando Gonzalez-Perez, Sandra Muntion, Jesus Garcia-Brinon, Maria-Carmen Garcia-Macias, Jose Carlos Rodriguez-Cabello, Miguel Pericacho, Matilde Alonso, Fermin Sanchez-Guijo
Summary: Combination of an elastin-like recombinamer (ELR)-based hydrogel with mesenchymal stromal cells (MSCs) shows potential in improving tissue reperfusion and blood vessel density in a murine hindlimb ischemia model, suggesting a promising approach for clinical application.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Fernando Gonzalez-Perez, Matilde Alonso, Israel Gonzalez de Torre, Mercedes Santos, Jose Carlos Rodriguez-Cabello
Summary: This study presents a 3D scaffold based on elastin-like recombinamer hydrogels for spatiotemporal control of vascularization and innervation in tissue regeneration. In vitro studies show the promotion of endothelial cell and axon spreading by the designed scaffold. Subcutaneous implantation experiments demonstrate the ability of the scaffold to induce angiogenesis and neurogenesis in predetermined areas.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Anna Majkowska, Karla E. Inostroza-Brito, Mariel Gonzalez, Carlos Redondo-Gomez, Alistair Rice, Jose Carlos Rodriguez-Cabello, Armando E. Del Rio Hernandez, Alvaro Mata
Summary: The study demonstrates the possibility to generate coassembling tubular membranes with distinct structures, properties, and bioactivity by minor modifications in the molecular structures of peptide amphiphiles and elastin-like recombinamers.
Review
Nanoscience & Nanotechnology
Rodrigo Curvello, Verena Kast, Paloma Ordonez-Moran, Alvaro Mata, Daniela Loessner
Summary: Biology can be used to design materials and approaches for tumour tissue engineering, and biomaterials are necessary for rebuilding tissue organization, composition, and function. This review discusses bioengineering strategies that recreate the pathophysiology of tumour tissues to address questions in cancer research. Tissue engineering has produced innovative tools for cancer research.
NATURE REVIEWS MATERIALS
(2023)
Meeting Abstract
Cell & Tissue Engineering
Sergio Acosta, Zhou Ye, Conrado Aparicio, Arturo Ibanez-Fonseca, Matilde Alonso, Jose Carlos Rodriguez-Cabello
TISSUE ENGINEERING PART A
(2022)