Review
Biotechnology & Applied Microbiology
Fatemeh Dadkhah Tehrani, Arezoo Firouzeh, Iman Shabani, Azadeh Shabani
Summary: The amniotic membrane, as the innermost layer of the fetal placenta, has unique properties that make it valuable in regenerative medicine applications. Beyond sterilization and preservation, additional modifications are being explored to enhance its properties and durability, presenting a new area of study in its applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Zeming Hu, Yang Luo, Renhao Ni, Yiwei Hu, Fang Yang, Tianyu Du, Yabin Zhu
Summary: This article reviews the potential and characteristics of the human amniotic membrane (hAM) in regenerative medicine applications. Firstly, the biological properties and stemness markers of hAM-derived cells are discussed, highlighting hAM as a promising source of stem cells. Then, current knowledge on the collection, preparation, preservation, and decellularization of hAM is systematically summarized, along with their roles in tissue engineering applications. Finally, the recent advances and modifications of hAM in regenerative medicine are highlighted, emphasizing the need for further research to enhance therapeutic effectiveness.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Agathe Gremare, Lisa Thibes, Maude Gluais, Yoann Torres, Diane Potart, Nicolas Da Silva, Nathalie Dusserre, Mathilde Fenelon, Loic Sentilhes, Sabrina Lacomme, Isabelle Svahn, Etienne Gontier, Jean-Christophe Fricain, Nicolas L'Heureux
Summary: An innovative approach using human amniotic membrane (HAM) and textile-inspired assembly strategy was proposed to develop biological vascular substitutes, avoiding costly tissue engineering methods. The study demonstrated that HAM-based tissue-engineered vascular grafts (TEVGs) exhibited clinically relevant mechanical properties and could be produced as off-the-shelf allogeneic implants.
Review
Biochemistry & Molecular Biology
Olivier Schussler, Pierre E. Falcoz, Juan C. Chachques, Marco Alifano, Yves Lecarpentier
Summary: The clinical impact of cell therapy after myocardial infarction is limited by low cell engraftment, cell death in inflammatory and poor angiogenic areas, and secondary migration. Enhancing integrin interaction with cells through a three-dimensional material, especially when applied epicardially, may improve outcomes. Using collagen as a polymer backbone for tissue engineering shows promise in creating contractile tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Mathilde Fenelon, Paul Galvez, Daniel Kalbermatten, Paolo Scolozzi, Srinivas Madduri
Summary: The amniotic membrane (AM) is a widely available and cost-effective tissue with unique biological properties. Advanced hybrid materials based on AMs have been developed by combining them with secondary materials. This review focuses on the increasing development of new multilayer composite-based AMs and highlights the benefits of additive manufacturing technologies and electrospinning in expanding their use for tissue engineering and clinical applications. The use of AMs and multilayer composite-based AMs in nerve regeneration is particularly emphasized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Anatomy & Morphology
Sumeyye Yuksel, Mehmet Dogan Asik, Halil Murat Aydin, Ergin Tonuk, Emin Yusuf Aydin, Murat Bozkurt
Summary: Decellularized amniotic membranes have potential as tissue engineering materials, providing scaffolds that support cell survival and adhesion with desired flexibility. The study demonstrated that multilayered decellularized amniotic membrane scaffolds can be effective in promoting cell viability and forming a flexible biomaterial with good handling properties.
Review
Biochemistry & Molecular Biology
Mathilde Fenelon, Sylvain Catros, Christophe Meyer, Jean-Christophe Fricain, Laurent Obert, Frederic Auber, Aurelien Louvrier, Florelle Gindraux
Summary: Human amniotic membrane (hAM) is a highly abundant and cost-effective biomaterial used as a scaffold in tissue engineering, providing a favorable environment for cell growth and differentiation. It possesses biocompatibility, low immunogenicity, adequate mechanical properties, and various therapeutic effects, making it a versatile material for tissue repair applications.
Article
Instruments & Instrumentation
Hamid Reza Aghayan, Motahareh Sheikh Hosseini, Mahdi Gholami, Fereshteh Mohamadi-jahani, Akram Tayanloo-Beik, Sepideh Alavi-Moghadam, Moloud Payab, Parisa Goodarzi, Mohammad Abdollahi, Bagher Larijani, Babak Arjmand
Summary: This study investigated the regenerative effects of tissue-engineered dressings composed of human amniotic membrane graft (AM) and two types of mesenchymal stem cells (MSCs) in a rat excisional wound model. The results demonstrated that MSC-seeded AMs had superior effects on wound closure rate, re-epithelialization, angiogenesis, and collagen remodeling compared to the control groups, with placenta-derived MSCs (PLMSCs) showing better healing effects. Seeding of MSCs on AM boosted its regenerative effects in wound treatment, with PLMSCs having superior effects to ADMSCs in the excisional wound rat model.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2022)
Article
Engineering, Biomedical
Bo Wang, Xiaolong Wang, Allen Kenneth, Alexander Drena, Arsenio Pacheco, Lindsey Kalvin, EI-Sayed Ibrahim, Peter J. Rossi, Kaitlyn Thatcher, Joy Lincoln
Summary: This study investigates the feasibility of constructing a small vascular graft from decellularized amniotic membranes (DAMs). The results demonstrate that the genipin-crosslinked DAM graft has a good structure, stability, mechanical property, and biocompatibility. After transplantation, the genipin-crosslinked DAM graft remains patent with endothelial and smooth muscle cell regeneration, suggesting its potential as a small tissue engineered graft for vascular transplantation in the future.
Review
Biotechnology & Applied Microbiology
Chloe D. Devillard, Christophe A. Marquette
Summary: The field of biofabrication has seen significant advancements in recent years, particularly in 3D bioprinting technologies. By combining improvements in fabrication methods, cell source availability, biomaterial formulation, and vascular tissue development, new strategies for creating blood vessels that meet clinical needs are being developed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Cell Biology
Mandana Kazem Arki, Kasra Moeinabadi-Bidgoli, Nikoo Hossein-Khannazer, Roberto Gramignoli, Mustapha Najimi, Massoud Vosough
Summary: The liver is responsible for various important functions in the body, and diseases affecting the liver can lead to inflammation, fibrosis, and eventually cirrhosis. The human amniotic membrane (hAM) has beneficial properties that can help treat chronic liver diseases, including its anti-fibrotic and anti-inflammatory effects. It can also be used as a scaffold for liver tissue engineering.
Review
Biotechnology & Applied Microbiology
Xinyu Wang, Vincent Chan, Peter R. Corridon
Summary: Vascular diseases cause severe damage and current treatments include medications, rehabilitation, and surgery. Vascular replacement therapy (VRT) is often required, but current methods have complications. Vascular tissue engineering using decellularization techniques shows promise to address these issues.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Anatomy & Morphology
Shahrzad Ashouri, Seyede Atefe Hosseini, Seyed Javad Hoseini, Fatemeh Tara, Alireza Ebrahimzadeh-Bideskan, Thomas J. Webster, Saeid Kargozar
Summary: This study aimed to develop detergent-free methods for the decellularization of human amniotic membrane (HAM) to make it suitable as inexpensive dressings for tissue engineering applications. Mechanical force (brushing) and physical treatment (heating) methods were compared with a chemical-based approach. All three methods effectively reduced the number of epithelial cells and the DNA content in HAM without compromising its integrity and key biological components. The physical method showed the greatest potential for cell proliferation.
Article
Engineering, Biomedical
Lennart P. Maljaars, Zeliha Guler, Jan-Paul W. R. Roovers, Deon Bezuidenhout
Summary: This study aims to produce an amniotic membrane (AM)-based scaffold that can withstand the mechanical loads applied in vesicovaginal fistula repair. Different strategies are investigated to improve the mechanical characteristics of AM. The composite constructs with AM showed sufficient mechanical characteristics for surgical implantation.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Engineering, Biomedical
Yuankun Zhai, Kevin Schilling, Tao Wang, Mirna El Khatib, Sergei Vinogradov, Edward B. Brown, Xinping Zhang
Summary: This study demonstrates the importance of spatiotemporal vessel type distribution in bone tissue engineering, providing new insights for devising more effective vascularization strategies. The research highlights the interdependent role of osteogenesis and angiogenesis, and the differential vascular response and vessel type distribution in healing and non-healing defects.
Article
Integrative & Complementary Medicine
Ya-Hsuan Lin, Hsein-Chang Wu, Po-Chun Hsieh, I-Shiang Tzeng, Shu-Ya Wu, Chan-Yen Kuo
Summary: The study aimed to identify the potential kernel acupoint combination in the treatment of dry eye disease (DED) through association rule mining based on extracted randomized controlled trials (RCTs) from a previous meta-analysis.
COMPLEMENTARY MEDICINE RESEARCH
(2021)
Article
Respiratory System
Shih-Hsing Yang, Mei-Chen Yang, Yao-Kuang Wu, Chih-Wei Wu, Po-Chun Hsieh, Chan-Yen Kuo, I-Shiang Tzeng, Chou-Chin Lan
Summary: The study investigated the association between work efficiency (WE) and exercise capacity, health-related quality of life (HRQL), and other cardiopulmonary parameters in 78 COPD patients. The results showed that patients with poor WE had lower exercise capacity, poorer HRQL, and more exertional dyspnea.
INTERNATIONAL JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
(2021)
Article
Integrative & Complementary Medicine
Ping-Hsun Lu, Jui-Lin Keng, Fu-Ming Tsai, Po-Hsuan Lu, Chan-Yen Kuo
Summary: By analyzing 17 randomized controlled trials on the treatment of diabetic gastroparesis using Apriori and frequent pattern growth algorithms, it was found that acupoints such as BL23, BL18, SP6, BL20, and PC6 are core elements in the treatment of DGP.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ching-Feng Wu, Ching-Yang Wu, Robin Y. -Y. Chiou, Wei-Cheng Yang, Chuen-Fu Lin, Chao-Min Wang, Po-Hsun Hou, Tzu-Chun Lin, Chan-Yen Kuo, Geng-Ruei Chang
Summary: The study showed that the treatment with Zotarolimus or Zotarolimus combined with 5-FU significantly inhibited lung adenocarcinoma growth, increased tumor cell apoptosis, reduced inflammation levels, and inhibited tumor metastasis. The synergistic anti-tumor effect of Zotarolimus and 5-FU on lung adenocarcinoma was evident, with the combination treatment being more effective than using 5-FU or Zotarolimus alone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chih-Hung Lin, Han-Fang Tseng, Po-Chun Hsieh, Valeria Chiu, Ting-Yun Lin, Chou-Chin Lan, I-Shiang Tzeng, Huan-Nung Chao, Chia-Chen Hsu, Chan-Yen Kuo
Summary: Chrysophanol has a potential protective effect against AKI by alleviating hypoxia/reoxygenation-induced kidney cell damage through regulating apoptosis, ER stress, and ferroptosis.
Article
Integrative & Complementary Medicine
Chih-Hung Lin, Ya-Hsuan Lin, I-Shiang Tzeng, Chan-Yen Kuo
Summary: This study identified potential kernel acupoint combinations for improving sleep quality by analyzing 26 acupoints from 32 eligible studies based on the Apriori algorithm. The top 10 most frequently selected acupoints included HT7, SP6, PC6, KI1, GV20, EM5, EX-HN3, EX-HN16, KI3, and MA-TF1. Relevant rules in the combination of acupoints were found to be (EX-HN3, EX-HN16)=>(GV20) and (HT7, KI1)=>(PC6) based on 21 association rules.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Integrative & Complementary Medicine
Ping-Hsun Lu, Yu-Yang Chen, Fu-Ming Tsai, Yuan-Ling Liao, Hui-Fen Huang, Wei-Hsuan Yu, Chan-Yen Kuo
Summary: Acupuncture has adjuvant benefits in improving simple obesity, with acupoint combinations being more effective. This study used the Apriori algorithm to discover association rules between acupuncture points and found the core acupoint combinations for treating simple obesity.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Integrative & Complementary Medicine
Chih-Hung Lin, Jiunn-Sheng Wu, Po-Chun Hsieh, Valeria Chiu, Chou-Chin Lan, Chan-Yen Kuo
Summary: In this study, it was found that wild bitter melon extract (WBM) exerted a protective effect against hypoxia-induced cell death in microglial cells by regulating ferroptosis, ER stress, and apoptosis. The results demonstrated that WBM reversed the effects of hypoxia on antiferroptotic molecules and alleviated hypoxia-induced apoptosis. These findings suggest that WBM may be a promising candidate for preventing CNS disorders in the future.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Ophthalmology
Robert H. Rosa Jr, Wankun Xie, Min Zhao, Shu-huai Tsai, Gavin W. Roddy, Maxwell G. Su, Luke B. Potts, Travis W. Hein, Lih Kuo
Summary: This study investigated the effect of STC-1 on photoreceptor degeneration in a porcine model of RP and found that it improved retinal function, preserved photoreceptor integrity, and reduced retinal degeneration through antioxidative and anti-inflammatory effects.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ping-Hsun Lu, Tzu-Hsien Liao, Yau-Hung Chen, Yeng-Ling Hsu, Chan-Yen Kuo, Chuan-Chi Chan, Lu-Kai Wang, Ching-Yuh Chern, Fu-Ming Tsai
Summary: This study found that six coumarin derivatives can inhibit platelet aggregation induced by ADP, with 7-hydroxyflavone having the most severe effect. In addition, these derivatives also affect the downstream signal transduction of the ADP receptor by inhibiting the release of calcium ions and the regulation of cAMP.
Article
Medicine, General & Internal
Chih-Wei Wu, Bach -Tung Pham, Jia-Ching Wang, Yao-Kuang Wu, Chan -Yen Kuo, Yi-Chiung Hsu
Summary: The impact of the first wave of the COVID-19 pandemic in Taiwan and the mortality risk factors among critically ill patients were investigated. An AI mortality prediction model using CXR alone was developed, exhibiting high accuracy. Factors such as septic shock and high BRIXIA score were associated with increased mortality risk.
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2023)
Review
Medicine, General & Internal
Po-Yu Chien, Chan-Yen Kuo, Meng-Hsuan Lin, Yao-Jen Chang, Chin-Chuan Hung
Summary: Narcolepsy is a neurological disease characterized by excessive daytime sleepiness. A network meta-analysis found that solriamfetol showed superior efficacy in improving symptoms compared to other medications.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Chan-Yen Kuo, Po-Chun Hsieh, Valeria Chiu, Chou-Chin Lan, Kuo-Cheng Lu
Summary: A previous study suggested that tissue inflammation and fibrosis play important roles in the development of ccRCC. This study aimed to investigate the mechanism of LCN-2-mediated ferroptosis and inflammation in vhl-mutated cells. The results demonstrated that VHL mutation increased ROS production and depressed GPX4 expression, while LCN-2 knockdown reversed these effects. Additionally, LCN-2 sensitized cells to inflammation and promoted M1-like polarization of macrophages.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2023)
Article
Chemistry, Medicinal
I-Ting Wu, Chan-Yen Kuo, Ching-Hui Su, Yu-Hsuan Lan, Chin-Chuan Hung
Summary: This study investigated the inhibitory effects of pinostrobin and tectochrysin on P-gp in MDR cancer cells and the underlying mechanisms. The results showed that these compounds were not substrates of P-gp and did not affect its expression, but they noncompetitively inhibited the efflux of rhodamine 123 and doxorubicin through P-gp, leading to the resensitization of MDR cancer cells. Therefore, pinostrobin and tectochrysin are effective P-gp inhibitors and potential candidates for resensitizing MDR cancer cells.
Article
Biochemistry & Molecular Biology
Po-Chih Hsu, Chia-Chen Hsu, Yi-Jan Hsia, Chan-Yen Kuo
Summary: This study found that chrysophanol can be used as a drug for treating oral cancer by modulating mTOR/PPAR-alpha and causing ROS accumulation to inhibit the growth of oral squamous cell carcinoma cells.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)