Article
Multidisciplinary Sciences
Wei Cao, Xiaohong Yang, Xiao Hua Hu, Jun Li, Jia Tian, RenJun OuYang, Xue Lin
Summary: This study analyzed the biological characteristics of the mandible and mandibular bone marrow mesenchymal stem cells in the bone microenvironment of postmenopausal osteoporosis. It identified miR-344d-3p as a promoter of osteogenic differentiation and inhibitor of adipogenic differentiation, with Dnmt3a potentially being its target gene. These findings are of significant importance in the field of bone reconstruction.
Article
Medicine, Research & Experimental
De-Yu Duan, Jing Tang, Hong-Tao Tian, Yang-Yang Shi, Jie Jia
Summary: Adipocyte-derived MVs-miR-148a promotes adipogenic differentiation and suppresses osteogenic differentiation by targeting the Wnt5a/Ror2 pathway.
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Chenglong Wang, Sabine Stoeckl, Shushan Li, Marietta Herrmann, Christoph Lukas, Yvonne Reinders, Albert Sickmann, Susanne Graessel
Summary: This study investigated the effects of extracellular vesicles (EVs) isolated from different stages of human bone marrow mesenchymal stem cells (hBMSCs) on the osteogenic and adipogenic differentiation capacity of naive hBMSCs. The results showed that EVs from late-stage osteogenic differentiated hBMSCs promoted osteogenic potential in naive hBMSCs and inhibited their adipogenic differentiation. Proteomic analysis revealed distinct protein profiles in EVs from osteogenic and naive hBMSCs.
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Chemistry, Applied
Na Ren, Xin Yu, Aizhu Wang, Na Liang, Zhichao Feng, Chunhui Sun
Summary: The study demonstrates that ScCl3 can enhance proliferation and osteogenic differentiation of rat bone MSCs while reducing adipogenic differentiation to a certain extent through the Wnt/beta-catenin signaling pathway. Appropriate concentrations of ScCl3 show potential in improving bone remodeling and may lead to new strategies for designing composites for biomedical applications in the future.
JOURNAL OF RARE EARTHS
(2022)
Article
Biochemistry & Molecular Biology
Luca Dalle Carbonare, Jessica Bertacco, Arianna Minoia, Mattia Cominacini, Lekhana Bhandary, Rossella Elia, Giovanni Gambaro, Monica Mottes, Maria Teresa Valenti
Summary: RUNX2 and SOX9 are key transcriptional regulators in chondrogenesis, with their activities being reciprocally regulated. The miR-204-5p has been shown to play a role in chondrogenesis, negatively regulating osteochondrogenic commitment of MSCs and positively regulating chondrocyte maturation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Hui Qin, Yuxin Niu, Haiyang Luan, Minghan Li, Lu Zheng, Yifan Pan, Wei Liu
Summary: This study found that PFASs have different adverse effects and relative potency on the multipotent differentiation of human mesenchymal stem cells, depending on the integrated action of three PPAR subtypes. The results contribute to a better understanding of the molecular initiating events of PFASs, explain the health issues caused by PFASs, and emphasize the necessity of risk assessment for alternatives.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Immunology
Huanhuan Li, Xueju Xu, Dao Wang, Yuan Zhang, Jiao Chen, Bai Li, Shufang Su, Linlin Wei, Hongliang You, Yingqi Fang, Yingchao Wang, Yufeng Liu
Summary: The study revealed that DNMT1 mediated the hypermethylation of MEG3 promoter in BMSCs of pediatric aplastic anemia, and the DNMT1/MEG3/BMP4 pathway modulated the osteogenic differentiation of BMSCs.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Thorben Sauer, Giulia Facchinetti, Michael Kohl, Justyna M. Kowal, Svitlana Rozanova, Julia Horn, Hagen Schmal, Ivo Kwee, Arndt-Peter Schulz, Sonja Hartwig, Moustapha Kassem, Jens K. Habermann, Timo Gemoll
Summary: This study characterized the differences between MSCs and cells after adipogenic or osteoblastic differentiation at the proteome level. Specific proteins related to bone formation were identified, which could be used for regenerative medicine in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Shin Ishikane, Eigo Ikushima, Kazunobu Igawa, Katsuhiko Tomooka, Fumi Takahashi-Yanaga
Summary: The study showed that DIF-1 significantly enhanced adipogenic differentiation but attenuated osteogenic differentiation of BM-MSCs. Results suggest that DIF-1 supports MSC differentiation towards the desired cell fate by enhancing differentiation efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Cell Biology
Qingyu Zhang, Jun Dong, Peng Zhang, Dongsheng Zhou, Fanxiao Liu
Summary: Key gene regulatory events were identified in early stages of lineage commitment in BMSCs, with two transcription factors (Tsc22d3 and Epas1) showing elevated expression throughout different stages of three-directional differentiation in rats.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yue Jing, Jian Zhou, Fenghua Guo, Lin Yu, Xiaomeng Ren, Xiushan Yin
Summary: This study found that betaine promotes osteogenic differentiation and inhibits adipogenic differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and human adipose-derived mesenchymal stem cells (hAD-MSCs). Further analysis showed that betaine exerts its effects through pathways such as the PI3K-Akt signaling pathway, cytokine-cytokine receptor interaction, and ECM-receptor interaction. The results contribute to the exploration of betaine as a potential adjunct for MSC therapy.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Min Wang, Tianming Dai, Qingqi Meng, Wen Wang, Siming Li
Summary: This study aimed to investigate the regulatory effects of miR-28 on osteogenic differentiation of hBMMSCs. It was found that miR-28 could inhibit STAT1 expression, upregulate AKP and RUNX2 expression, and promote osteogenic differentiation.
Article
Cell & Tissue Engineering
You Zhou, Siyu Liu, Wei Wang, Qiang Sun, Mengzhu Lv, Shude Yang, Shuang Tong, Shu Guo
Summary: miR-204-5p regulates osteogenic differentiation of hADSCs by suppressing FOXC1 and GDF7 expression, FOXC1 promotes deacetylation of miR-204-5p to enhance GDF7 expression, while GDF7 induces hADSC osteogenesis differentiation through the AKT and P38 signaling pathways.
STEM CELL RESEARCH & THERAPY
(2021)