Review
Cell Biology
Michael L. Jennings
Summary: The major transmembrane protein of the red blood cell, band 3, plays a crucial role in Cl⁻/HCO₃⁻3 exchange and anchoring the membrane skeleton. Research on band 3 has provided insights into protein structure, ion transport mechanisms, and its involvement in cell abnormalities and acidosis. The identification of genetic variants and crystal structure information have paved the way for detailed molecular models of transport and integrative relationships with other molecules during gas exchange in capillaries.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Satoko Iwahori, Angie C. Umana, Robert F. Kalejta, Takayuki Murata
Summary: In the context of human cytomegalovirus infection, the interaction between 14-3-3 proteins and UL97 stabilizes the activity of UL97. This interaction is kinase activity-dependent.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Katherine E. Badior, Joseph R. Casey
Summary: Band 3, the predominant protein in erythrocyte membranes, undergoes significant conformational dynamics between intracellular and extracellular regions, potentially playing a crucial role in erythrocyte aging.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Hematology
Samantha Weber-Fishkin, Harrison S. Seidner, Geoffrey Gunter, Mary D. Frame
Summary: This study aims to investigate the kinetics of erythrocyte aggregation under different temperature and oxygen conditions. The results showed that erythrocytes aggregate faster and with a higher magnitude in hyperthermia and hypoxemia, especially in autologous plasma. Furthermore, the C-terminal domain of band 3 plays a pivotal role in erythrocyte aggregation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biochemistry & Molecular Biology
Panae Noomuna, John M. Hausman, Ruhani Sansoya, Theodosia Kalfa, Mary Risinger, Philip S. Low
Summary: Proteolysis of the major erythrocyte tyrosine phosphatase PTP1B leads to increased tyrosine phosphorylation in sickle cells, which weakens the erythrocyte membrane and causes release of membrane vesicles and cell free hemoglobin, contributing to the symptoms of sickle cell disease.
Article
Cell Biology
Xiaofang Shi, Qi Zhang, Jie Li, Xingyu Liu, Yi Zhang, Minhua Huang, Weiqing Fang, Junyu Xu, Tifei Yuan, Lin Xiao, Yi-Quan Tang, Xiao-Dong Wang, Jianhong Luo, Wei Yang
Summary: Phosphorylation levels of GluN2B-Y1070 in the mPFC are linked to depressive behaviors, blocking its phosphorylation can have antidepressant-like effects without affecting cognitive or anxiety-related behaviors in mice. Additionally, the Y1070F mutation helps increase excitatory synapse numbers, but only in the layer 5 pyramidal neurons of the mPFC.
Article
Multidisciplinary Sciences
Xinxin Chen, Jianchao Zhang, Pulan Liu, Yangyang Wei, Xi'e Wang, Junyu Xiao, Chih-chen Wang, Lei Wang
Summary: This study reveals a unique mechanism for the secretion and activation of Fam20C, showing that only mature Fam20C processed by S1P functions in osteoblast differentiation and mineralization, providing a molecular link between biomineralization and lipid metabolism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Yuhao Qiang, Jia Liu, Ming Dao, E. Du
Summary: Red blood cells are exposed to varying shear stress and oxygen tension during circulation, which can affect their biomechanics. Cyclic hypoxia alone significantly reduces cell deformability, particularly in sickle cells which show less resistance to fatigue damage. Treatment with an anti-sickling drug can improve the fatigue resistance of sickle cells exposed to cyclic hypoxia.
Article
Multidisciplinary Sciences
Yu-Lin Yang, Li -Bo Cao, Wen-Rui He, Li Zhong, Yi Guo, Qing Yang, Hong -Bing Shu, Ming-Ming Hu
Summary: The current understanding of nucleic acid-mediated innate immunity is that the binding of intracellular sensors to nucleic acids is enough to activate them. However, this study reveals that the endocytosis of viruses or foreign DNA provides a priming signal for the activation of the DNA sensor cGAS in the innate immune response. This finding demonstrates that the activation of cGAS following endocytosis requires tyrosine phosphorylation mediated by the V-ATPase-SYK pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Cara Abecunas, Christopher E. Whitehead, Elizabeth K. Ziemke, Douglas G. Baumann, Christy L. Frankowski-McGregor, Judith S. Sebolt-Leopold, Mohammad Fallahi-Sichani
Summary: NF1 loss of function mutations are common in melanoma, causing hyperactivated RAS and tumor growth; NF1(LoF) melanoma cells show inconsistent sensitivity to MEK, ERK or PI3K/mTOR inhibitors; a targeted kinase inhibitor screen identified MTX-216 as highly effective in blocking NF1(LoF) melanoma growth.
Article
Microbiology
Chenyao Wang, Nikhil Sharma, Manoj Veleeparambil, Patricia M. Kessler, Belinda Willard, Ganes C. Sen
Summary: The nature and intensity of the innate immune response to virus infection are critical for the course of pathogenesis in the host. STING, an endoplasmic reticulum-associated protein, plays a key role in triggering responses to microbial or cellular cytoplasmic DNA. Phosphorylation of Tyr240 of STING by the tyrosine kinase Syk is essential for all signaling activities of STING, and its signaling is necessary for interferon induction.
Article
Oncology
Anuli C. Uzozie, Enes K. Ergin, Nina Rolf, Janice Tsui, Amanda Lorentzian, Samuel S. H. Weng, Lorenz Nierves, Theodore G. Smith, C. James Lim, Christopher A. Maxwell, Gregor S. D. Reid, Philipp F. Lange
Summary: Global protein abundance, phosphorylation, and protein maturation were studied in pediatric B- and T-cell ALL patients and corresponding xenografts, revealing that genomic and host factors shape protein and post-translational modification landscapes differently. The study highlighted areas of diverging biology and confirmed murine patient-derived xenografts as an accurate model system.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Juan C. Moreno, Bruno E. Rojas, Ruben Vicente, Michal Gorka, Timon Matz, Monika Chodasiewicz, Juan S. Peralta-Ariza, Youjun Zhang, Saleh Alseekh, Dorothee Childs, Marcin Luzarowski, Zoran Nikoloski, Raz Zarivach, Dirk Walther, Matias D. Hartman, Carlos M. Figueroa, Alberto A. Iglesias, Alisdair R. Fernie, Aleksandra Skirycz
Summary: The study identifies a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, that enhances plant tolerance to oxidative stress by interfering with glucose metabolism. This suggests that proteogenic dipeptides may act as crucial regulators in stress responses and metabolism.
Article
Cell Biology
Shasha Liu, Yuan Liao, Biao Chen, Yuhai Chen, Ziding Yu, Haitao Wei, Lianfeng Zhang, Shile Huang, Paul B. Rothman, George Fu Gao, Ji-Long Chen
Summary: Research has shown that activation of STAT1 in early viral infection is not dependent on cytokines and JAKs, but mainly through Syk. Deletion of Syk profoundly impairs immediate innate immunity and suppresses the antiviral response.
Article
Biochemistry & Molecular Biology
Zuzana Trosanova, Petr Lousa, Aneta Kozelekova, Tomas Brom, Norbert Gasparik, Jan Tungli, Veronika Weisova, Erik Zupa, Gabriel Zoldak, Joze Hritz
Summary: This study describes the equilibrium between monomers, homo- and heterodimers of 14-3-3ζ isoform in the unmodified and phosphorylated form. The researchers found that phosphorylation of 14-3-3ζ at Ser58 significantly increases its homodimeric K-d value. Various experimental methods were used to efficiently monitor the dimerization of 14-3-3ζ protein and observe the effects of external factors and client protein binding.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Review
Obstetrics & Gynecology
G. Buzzaccarini, L. Marin, M. Noventa, A. Vitagliano, A. Riva, F. Dessole, G. Capobianco, L. Bordin, A. Andrisani, G. Ambrosini
Summary: This study conducted a systematic review on the effects of hyaluronic acid in improving vulvovaginal pathology symptoms, finding hyaluronic acid generally effective as a moisturizer for women who cannot undergo estrogen therapy due to contraindications or are experiencing symptoms affecting sexual well-being. Further well-designed randomized controlled trials are needed to confirm its efficacy.
Article
Biochemistry & Molecular Biology
Chiara Sabbadin, Alessandra Andrisani, Gabriella Dona, Elena Tibaldi, Anna Maria Brunati, Stefano Dall'Acqua, Eugenio Ragazzi, Guido Ambrosini, Decio Armanini, Luciana Bordin
Summary: This study found that licorice intake can decrease oxidative damage to RBCs caused by DDS-NHOH, and this reduction is directly related to plasma GA concentration. Therefore, GA may be considered a new potential tool for preventing severe endometriosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Peripheral Vascular Disease
Decio Armanini, Chiara Sabbadin, Luciana Bordin
JOURNAL OF CLINICAL HYPERTENSION
(2021)
Letter
Andrology
Loris Marin, Guido Ambrosini, Ludovica Nuzzi, Luciana Bordin, Alessandra Andrisani
Editorial Material
Peripheral Vascular Disease
Decio Armanini, Marco Boscaro, Chiara Sabbadin, Luciana Bordin
JOURNAL OF CLINICAL HYPERTENSION
(2022)
Article
Oncology
Federica Frezzato, Andrea Visentin, Filippo Severin, Serena Pizzo, Edoardo Ruggeri, Nayla Mouawad, Leonardo Martinello, Elisa Pagnin, Valentina Trimarco, Alessia Tonini, Samuela Carraro, Stefano Pravato, Silvia Imbergamo, Sabrina Manni, Francesco Piazza, Anna Maria Brunati, Monica Facco, Livio Trentin
Summary: The use of ibrutinib has significantly changed the management of chronic lymphocytic leukemia (CLL) patients, but resistance to treatment can occur. High levels of HSP70 and its regulator HSF1 have been found in patients experiencing ibrutinib failure, suggesting their involvement in drug resistance mechanisms. Inhibitors targeting the HSP70/HSF1 axis may be a novel therapeutic strategy to overcome ibrutinib resistance in CLL patients.
Article
Biochemistry & Molecular Biology
Giuseppe Cannino, Andrea Urbani, Marco Gaspari, Mariaconcetta Varano, Alessandro Negro, Antonio Filippi, Francesco Ciscato, Ionica Masgras, Christoph Gerle, Elena Tibaldi, Anna Maria Brunati, Giorgio Colombo, Giovanna Lippe, Paolo Bernardi, Andrea Rasola
Summary: This study reveals that the mitochondrial chaperone TRAP1 interacts with F-ATP synthase, increasing its catalytic activity and counteracting the inhibitory effect of CyPD. Additionally, TRAP1 directly inhibits the channel activity of F-ATP synthase and reverses PTP induction by CyPD, thus preventing mitochondrial depolarization and cell death. These findings highlight the importance of TRAP1 in cell bioenergetics and survival.
CELL DEATH AND DIFFERENTIATION
(2022)
Letter
Endocrinology & Metabolism
Decio Armanini, Chiara Sabbadin, Luigi De Marco, Luciana Bordin
Summary: A recent study found that women with polycystic ovary syndrome (PCOS) have a 52% increased risk of COVID-19 infection, with nearly twice the incidence compared to women without PCOS. A survey of 200 unvaccinated women with PCOS who had been treated with spironolactone for more than 4 months revealed only four infected patients, with mild or minimal symptoms. Further research is needed to confirm these findings and evaluate the use of spironolactone in both PCOS and the general population for COVID-19.
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Decio Armanini, Marco Boscaro, Luciana Bordin, Chiara Sabbadin
Summary: PCOS is a heterogeneous and common disease with symptoms varying with age, typically characterized by hyperandrogenism, chronic oligo-anovulation, and metabolic disorders. Treatment involves lifestyle improvements and medications tailored to individual cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Chiara Sabbadin, Francesca Beggiao, Carlotta Keiko Vedolin, Gloria Orlando, Eugenio Ragazzi, Filippo Ceccato, Mattia Barbot, Luciana Bordin, Gabriella Dona, Alessandra Andrisani, Anna Belloni Fortina, Carla Scaroni, Decio Armanini
Summary: This study demonstrates that spironolactone is a safe and effective treatment for hyperandrogenic skin disorders, particularly in patients with polycystic ovary syndrome. The medication can provide long-lasting improvement in symptoms, even after discontinuation for several months.
ENDOCRINE METABOLIC & IMMUNE DISORDERS-DRUG TARGETS
(2023)
Editorial Material
Peripheral Vascular Disease
Decio Armanini, Luciana Bordin, Jacopo Manso, Marco Boscaro, Chiara Sabbadin
JOURNAL OF CLINICAL HYPERTENSION
(2022)
Review
Oncology
Mario Angelo Pagano, Federica Frezzato, Andrea Visentin, Livio Trentin, Anna Maria Brunati
Summary: Phosphorylation is a crucial modification that regulates cellular functions, and its dysregulation is observed in chronic lymphocytic leukemia (CLL). Reactive oxygen species (ROS) and phosphorylation cooperate to support the survival of CLL cells. ROS and phosphorylation mainly influence the balance between redox homeostasis and transcription factors.
Meeting Abstract
Psychiatry
C. Idotta, E. Tibaldi, N. Favaretto, M. Pagano, R. Peruzzo, G. Pigato, D. Cazzador, P. Meneguzzo, M. Solmi, L. Leanza, A. Favaro, A. M. Brunati, T. Toffanin
EUROPEAN PSYCHIATRY
(2021)
Meeting Abstract
Obstetrics & Gynecology
M. Noventa, A. Riva, G. Buzzaccarini, L. Marin, C. Sabbadin, L. Bordin, M. Menegazzo, G. Ambrosini, A. Andrisani
HUMAN REPRODUCTION
(2021)