Article
Biochemistry & Molecular Biology
Mauro Giorgi, Adriana Erica Miele, Silvia Cardarelli, Alessandra Giorgi, Mara Massimi, Stefano Biagioni, Michele Saliola
Summary: Phosphodiesterases (PDEs) are a superfamily of enzymes that hydrolyze cyclic nucleotides (cAMP/cGMP) and play important roles in cell life. The cGMP-dependent PDE5 is the major enzyme in many mammalian tissues, regulating various cellular and tissue processes. Using Kluyveromyces lactis as a model organism, the roles of different isoforms (PDE5A1, A2, and A3) in cellular redox metabolism were studied, revealing their distinct functions. The study also identified the N-terminal peptide responsible for the assembly and mitochondrial localization of PDE5A1 and PDE5A2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Physics, Condensed Matter
Sriram Krishnamurthy, Remya Ann Mathews Kalapurakal, Ethayaraja Mani
Summary: This review highlights recent advances in computer simulations of colloidal self-assembly driven by anisotropic or orientation-dependent inter-particle interactions and discusses key challenges and opportunities in this field.
JOURNAL OF PHYSICS-CONDENSED MATTER
(2022)
Article
Chemistry, Physical
Vikram Singh Raghuwanshi, Christine Browne, Warren Batchelor, Gil Garnier
Summary: The self-assembly (SA) of cellulose nanocrystals (CNC) in suspensions is investigated, and it is found that CNC rods of different lengths exhibit different self-assembly behavior. Short rods form an isotropic phase at lower concentrations and become aligned in a nematic phase as the concentration increases. The interrod distance follows different power law slopes at different concentrations. In contrast, long rods remain in the isotropic phase throughout the concentration range. The SA and ordering of rods are influenced by the balance between attractive and repulsive forces. This understanding enables the engineering of CNC composites with unique optical properties for applications in sensors and bio-diagnostics.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Polymer Science
Guoxing Liao, Lei Chen, Yunjie Zhang, Oleksandr O. Mykhaylyk, Paul D. Topham, Daniel T. W. Toolan, Matthew J. Derry, Jonathan R. Howse, Qianqian Yu, Guiju Feng, Linge Wang
Summary: Block copolymer-based nanoproducts have great potential in high-end applications, but there is a lack of detailed studies on the nanofabrication process and the monitoring of concomitant self-assembled structural transitions. This study utilizes time-resolved small-angle X-ray scattering to track the nanostructural changes. The results provide guidelines for accessing various nanostructures from self-assembling block copolymers.
Article
Chemistry, Physical
Rafael V. M. Freire, Yeny Pillco-Valencia, Gabriel C. A. da Hora, Madeleine Ramstedt, Linda Sandblad, Thereza A. Soares, Stefan Salentinig
Summary: This study investigates the interactions between LL-37 and POPG vesicles, revealing that LL-37 actively modifies membrane curvature and leads to structural transformations from vesicles to mixed micelles. The findings shed light on the mechanisms underlying the interactions between LL-37 and bacteria mimetic vesicles, providing guidance for the development of AMP-based antimicrobial materials and therapies.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Physical
Niki Baccile, Alexandre Poirier, Patrick Le Griel, Petra Pernot, Melike Pala, Sophie Roelants, Wim Soetaert, Christian V. Stevens
Summary: This study investigates the structure-property relationship of different types of sophorolipids and sophorosides in water and identifies the chemical groups in their molecular structure that have a critical influence on their self-assembly properties. The number of sugar groups, pH, unsaturation, and lactonization were found to have a critical impact on sophorolipid self-assembly. The chemical nature of the end-chain functional group and chain length were also found to have a potentially critical impact.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Nanoscience & Nanotechnology
Vignesh Suresh, Ah Bian Chew, Christina Yuan Ling Tan, Hui Ru Tan
Summary: Block copolymer self-assembly enables the fabrication of complex multicomponent nanoarrays, which are rarely achieved by other nanofabrication approaches and have great potential.
Article
Materials Science, Multidisciplinary
M. R. Gazizov, A. O. Boev, C. D. Marioara, R. Holmestad, D. A. Aksyonov, M. Yu Gazizova, R. O. Kaibyshev
Summary: The atomic structure of omega plates forming on {111}Al planes in an Al-Cu-Mg-Ag alloy has been investigated, revealing volumetric and structural incompatibilities with the Al matrix. Different thicknesses of omega plates were studied under peak-aged conditions, showing shear components and misfit strains affecting plate interfaces and the thickening of omega precipitates.
MATERIALS CHARACTERIZATION
(2021)
Article
Chemistry, Physical
Gal Yosefi, Nitsan Eliraz, Sofiya Kolusheva, Hanna Rapaport, Ronit Bitton
Summary: This study reports on the formation and characterization of self-assembled membranes made with & lambda;-carrageenan and cationic & beta;-sheet peptides. The results show that the polysaccharide conformation, solution viscosity, and interactions between polysaccharides and peptides affect the properties of the membranes. The interplay between polymer-peptide interactions and polysaccharide chain stiffness could be used to control the structure-function relationship of the membranes.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Multidisciplinary Sciences
Aya Okuda, Masahiro Shimizu, Ken Morishima, Rintaro Inoue, Nobuhiro Sato, Reiko Urade, Masaaki Sugiyama
Summary: Multi-domain proteins exhibit various domain conformations under physiological conditions to regulate their functions through conformational changes. ER-60, a typical MDP protein folding enzyme, is found to have different domain conformations in solution depending on the redox state, with oxidized and reduced ER-60 structures differing from each other. Structural modeling with coarse-grained molecular dynamics simulation indicates that the distance between the two edge domains of oxidized ER-60 is longer and one edge domain has a more flexible conformation compared to reduced ER-60.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Vanessa B. Leffler, Sascha Ehlert, Beate Forster, Martin Dulle, Stephan Foerster
Summary: A defined three-dimensional nanoparticle assembly within unit cells can be achieved by linking nanoparticles to block copolymers. The study shows that the block length ratio and interfacial curvature are crucial factors determining lattice type, with even a one percent volume fraction enough to destabilize the structure and favor the formation of highly curved interfaces. This research paves the way for functional 3D-nanocomposites with nanoparticles located on specific matrix positions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Polymer Science
Artem Petrov, Alexander V. Chertovich, Alexey A. Gavrilov
Summary: This study obtained phase diagrams of atom transfer radical polymerization-induced self-assembly (ATRP PISA) through simulations and investigated the influence of ATRP's chemical nature on the self-assembly of diblock copolymers. It was found that chain growth termination via recombination played a key role in determining the ATRP PISA phase diagrams, and a dependence of aggregate morphology on concentration was observed.
Article
Chemistry, Multidisciplinary
Makoto Suzuki, Tsuyoshi Orido, Atsushi Takano, Yushu Matsushita
Summary: A quasicrystalline tiling pattern with tile size of ca. 60 nm has been discovered in a four-component pentablock polymer molecule. The structure was confirmed through TEM observation and SAXS diffraction patterns.
Article
Chemistry, Multidisciplinary
Flavia E. Galdino, Agustin S. Picco, Larissa B. Capeletti, Jefferson Bettini, Mateus B. Cardoso
Summary: In this study, synchrotron-based small-angle X-ray scattering and transmission electron microscopy techniques were used to track and estimate the formation and binding parameters of the protein corona, while visualizing its unstained hard and soft fractions. This strategy shows great potential for understanding fundamental aspects of the protein corona and can contribute to rational medical nanoparticle engineering.
Article
Mathematics
Alexandru Amarioarei, Frankie Spencer, Gefry Barad, Ana-Maria Gheorghe, Corina Itcus, Iris Tusa, Ana-Maria Prelipcean, Andrei Paun, Mihaela Paun, Alfonso Rodriguez-Paton, Romica Trandafir, Eugen Czeizler
Summary: Recent advances in computational modeling and simulation have brought computer scientists into the forefront of engineering new nanomaterials and nanodevices. The intrinsic self-assembly properties of DNA have been proven highly algorithmic and programmable, making it a versatile and functional nanomaterial. By studying the assembly characteristics of DNA and other bio-nanomaterials, potential for more effective control over structural patterns is uncovered.
Article
Dentistry, Oral Surgery & Medicine
Julia Lubauer, Renan Belli, Herwig Peterlik, Katrin Hurle, Ulrich Lohbauer
Summary: Lithium-based glass-ceramics are widely used in dental restorative materials. This study characterized the microstructure, crystalline fraction, glass chemistry, and mechanical properties of eleven commercial dental lithium-based glass ceramics. The results showed significant differences in chemical composition, microstructure, crystallinity, and mechanical properties among these materials. The findings provide valuable information for dentists to make informed choices.
Article
Biochemical Research Methods
Narges Lali, Peter Satzer, Alois Jungbauer
Summary: The trend in the biopharmaceutical industry is shifting towards continuous manufacturing, requiring traceability of materials. Understanding process dynamics and residence time distribution (RTD) is important for continuous biomanufacturing. In this study, the RTD of three-column periodic counter-current chromatography (PCC) was investigated using a fluorescent-labeled antibody as an inert tracer.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Leo A. A. Jakob, Tomas Mesurado, Alois Jungbauer, Nico Lingg
Summary: The CASPON enzyme is an interesting enzyme for fusion protein processing due to its generation of an authentic N-terminus. However, the high cysteine content in CASPON may lead to aggregation through disulfide-bond formation, which can negatively impact enzymatic activity. Different multimerization states of CASPON were isolated and analyzed to determine their propensity for multimerization and enzymatic activity. The results showed that the dimer state was the most stable and active, with 50% higher enzymatic activity than the tetramer state. Multimerization was mainly mediated by cysteine, and attractive protein-protein interactions were observed in the presence of ammonium sulfate and on a chromatographic stationary phase. These findings provide insights into protein purification and highlight the importance of controlling multimerization to maintain protein activity.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Baubek Spanov, Oladapo Olaleye, Tomes Mesurado, Natalia Govorukhina, Alois Jungbauer, Nico C. van de Merbel, Nico Lingg, Rainer Bischoff
Summary: The study found that Pertuzumab undergoes changes in charge heterogeneity, primarily due to deamidation in the Fc domain and N-terminal pyroglutamate formation in the heavy chain. Peptide mapping analysis also showed that clinical samples had 2-3% deamidation in the heavy chain CDR2, 20-25% deamidation in the Fc domain, and 10-15% N-terminal pyroglutamate formation in the heavy chain.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Magdalena Pappenreiter, Benjamin Bayer, Marijan Logarusic, Bernhard Sissolak, Alois Jungbauer
Summary: The study investigated the individual impacts of mild and rigorous intra-experimental parameter shifts on the culture performance and cell metabolism of an antibody producing CHO cell line. The results showed significant changes in cell growth, metabolism, and productivity due to intra-experimental parameter shifts. However, if the conditions within an experiment are changed, rigorous parameter shifts should be avoided, as they can lead to irreversible changes in cell behavior, as observed in media blending experiments.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Viktoria Mayer, Anna-Carina Frank, Shirin Preinsperger, Patrick Csar, Petra Steppert, Alois Jungbauer, Patricia Pereira Aguilar
Summary: Host cell DNA, especially chromatin, is an important impurity in downstream processing of enveloped viruses. Endonuclease treatment, using salt-tolerant endonucleases, combined with flow-through chromatography using core-shell particles, showed high purity and purification efficiency in the processing of recombinant measles virus. This strategy has the potential to be applied in other viral platforms.
BIOTECHNOLOGY PROGRESS
(2023)
Article
Biotechnology & Applied Microbiology
Magdalena Pappenreiter, Hubert Schwarz, Bernhard Sissolak, Alois Jungbauer, Veronique Chotteau
Summary: This study tested different process set ups and conditions in low shear TFF systems using magnetic levitation pumps in small-scale perfusion bioreactors. The authors validated a novel concept of applying reverse flow across the hollow fiber using two magnetically levitating pumps. The results showed that product sieving could be improved by 30% with the application of dynamic recirculation flow, and minimal product retention was achieved by reversing the flow of two alternating pumps with short cycle times. Additionally, a correlation was found between the passage of high molecular weight species and the flow direction, as well as the degree of product sieving.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Astrid Duerauer, Alois Jungbauer, Theresa Scharl
Summary: The biopharmaceutical industry is still running in batch mode due to heavy regulation, but there are initiatives and technologies being developed to move towards automated and autonomous bioprocessing. These include Process Analytical Technologies, Quality by Design, and Continuous Integrated Manufacturing. The ultimate goal is to achieve real-time release and establish a highly predictive and robust biomanufacturing system.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Gabriele Recanati, Magdalena Pappenreiter, Christoph Gstoettner, Patrick Scheidl, Elena Dominguez Vega, Bernhard Sissolak, Alois Jungbauer
Summary: Continuous precipitation coupled with continuous tangential flow filtration is an economical method for capturing recombinant antibodies from cell culture supernatant. By adopting tubular reactors to replace surge tanks, a continuous harvest and mass flow of product can be achieved with a narrow residence time distribution. The combination of CaCl2 precipitation and PEG capture method allows for efficient removal of host-cell DNA and retention of glycosylation profile.
ENGINEERING IN LIFE SCIENCES
(2023)
Article
Biochemical Research Methods
Markus Mozgovicz, Andreas Fischer, Cecile Brocard, Alois Jungbauer, Nico Lingg
Summary: Immobilized metal affinity chromatography (IMAC) is a powerful technique for capturing and purifying biopharmaceuticals. Protein recovery can be compromised due to spreading and unfolding of the protein, leading to fouling of the stationary phase. This study investigates the kinetics of irreversible adsorption of a protease on an IMAC resin and suggests that L-arginine sulfate can decelerate the irreversible adsorption.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemistry & Molecular Biology
Nico Lingg, Markus Mozgovicz, Theresa Schaufler, Alexander Prokup, Amy Carruthers, David Boerema, Laura Keigher, Nathan Brinkman, Alois Jungbauer
Summary: Currently, IVIG is manufactured in batch through cold ethanol precipitation, which leads to a slow process with high space requirements. We have developed a continuous method using a tubular reactor to replace the batch precipitation method. This reactor, filled with static mixers for efficient cooling, allows for complete precipitation with the same yield and purity even with a residence time of only one minute. A tubular reactor with specific dimensions can process 4000 L of plasma per day, providing an opportunity to reduce the plant footprint or intensify existing manufacturing plants.
PROCESS BIOCHEMISTRY
(2023)
Article
Engineering, Chemical
Mafalda S. Santos, Christian Schuster, Harald Rennhofer, Helga C. Lichtenegger, Herwig Peterlik, Tim Causon, Alois Jungbauer
Summary: Efficient recovery of building-block chemicals, such as biobased itaconic acid, produced by microbial processes can be achieved using ultrathin membranes. The composition of the membranes was optimized to improve the separation performance, and membranes with high selectivity for itaconic acid were successfully demonstrated in experiments with synthetic solutions and culture supernatant.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Baubek Spanov, Oladapo Olaleye, Tomes Mesurado, Natalia Govorukhina, Alois Jungbauer, Nico C. van de Merbel, Nico Lingg, Rainer Bischoff
Summary: The ion-exchange profile of pertuzumab was evaluated, revealing that deamidation in the Fc domain and N-terminal pyroglutamate formation in the heavy chain were the main contributors to charge heterogeneity. Peptide mapping analysis of clinical samples showed deamidation rates of 2-3% in the heavy chain CDR2, 20-25% in the Fc domain, and N-terminal pyroglutamate formation rates of 10-15% in the heavy chain. These findings suggest that in vitro stress studies can predict in vivo modifications.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Hannah Engelmaier, Christoph Dattenboeck, Katerina Petrushevska-Seebach, Alois Jungbauer, Nikolaus Hammerschmidt
Summary: The study presents an integrated, continuous process for the capture of recombinant blood coagulation factors. The developed process consists of three continuous unit operations and has demonstrated successful continuous capture of the factors over a 24-hour duration.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Hipassia M. Moura, Herwig Peterlik, Miriam M. Unterlass
Summary: This study presents a one-pot hydrothermal synthesis of inorganic-organic hybrid materials, achieving the concomitant synthesis, nanoscopic interpenetration, and covalent linking of components, leading to synergistic materials properties and improved thermal stability in PBI@SiO2 class II hybrids. The hybrids are utilized as heterogeneous and reusable photoredox catalysts for the reduction of aryl halides, demonstrating good optoelectronic properties. Small-angle X-ray scattering and powder X-ray diffraction revealed the existence of ordered intermediates in the synthesis process, shedding light on the structure formation mechanisms in sol-gel processes.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)