Optimization of methods for the genetic modification of human T cells

Precise gene deletion and replacement using the CRISPR/Cas9 system in human cells

(2016) Qiupeng Zheng et al.   BIOTECHNIQUES

GADS is required for TCR-mediated calcium influx and cytokine release, but not cellular adhesion, in human T cells

(2015) Mahmood Y. Bilal et al.   CELLULAR SIGNALLING

TCR-mediated functions are enhanced in activated peripheral blood T cells isolated from leucocyte reduction systems

(2015) Mikaela M. Tremblay et al.   JOURNAL OF IMMUNOLOGICAL METHODS

GRB2 Nucleates T Cell Receptor-Mediated LAT Clusters That Control PLC-γ1 Activation and Cytokine Production

(2015) Mahmood Yousif Bilal et al.   Frontiers in Immunology

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

(2014) Pankaj K. Mandal et al.   Cell Stem Cell

The impact of Nucleofection® on the activation state of primary human CD4 T cells

(2014) Mingce Zhang et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

(2013) Leonardo Chicaybam et al.   PLoS One

Conservation and divergence in the transcriptional programs of the human and mouse immune systems

(2013) T. Shay et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

(2011) Devaki Bhaya et al.   Annual Review of Genetics

Current prospects for RNA interference-based therapies

(2011) Beverly L. Davidson et al.   NATURE REVIEWS GENETICS

Polymerase II Promoter Strength Determines Efficacy of microRNA Adapted shRNAs

(2011) Robert Jan Lebbink et al.   PLoS One

Mammalian cell transfection: the present and the future

(2010) Tae Kyung Kim et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Of mice and men: Human RNA polymerase III promoter U6 is more efficient than its murine homologue for shRNA expression from a lentiviral vector in both human and murine progenitor cells

(2010) Roland Roelz et al.   EXPERIMENTAL HEMATOLOGY

Creating Higher Titer Lentivirus with Caffeine

(2010) Brian L. Ellis et al.   HUMAN GENE THERAPY

Grb2 functions at the top of the T-cell antigen receptor-induced tyrosine kinase cascade to control thymic selection

(2010) I. K. Jang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multipurpose modular lentiviral vectors for RNA interference and transgene expression

(2009) Venu Kesireddy et al.   MOLECULAR BIOLOGY REPORTS

Comparison of T Cell Receptor-Induced Proximal Signaling and Downstream Functions in Immortalized and Primary T Cells

(2009) Rebekah R. Bartelt et al.   PLoS One

Comparative Proteomic Phenotyping of Cell Lines and Primary Cells to Assess Preservation of Cell Type-specific Functions

(2008) Cuiping Pan et al.   MOLECULAR & CELLULAR PROTEOMICS

Rational Design Leads to More Potent RNA Interference Against Hepatitis B Virus: Factors Effecting Silencing Efficiency

(2008) Kathy Keck et al.   MOLECULAR THERAPY

Artificial miRNAs mitigate shRNA-mediated toxicity in the brain: Implications for the therapeutic development of RNAi

(2008) J. L. McBride et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA