Journal
IMMUNOGENETICS
Volume 68, Issue 1, Pages 43-54Publisher
SPRINGER
DOI: 10.1007/s00251-015-0881-y
Keywords
miR-27a; miR-214; Th17 cells; miRNA; Differentiation; Relapsing-remitting multiple sclerosis
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Multiple sclerosis (MS) is one of the most prevalent autoimmune diseases, which involves the central nervous system. In this illness, Treg/Th17 cell imbalance causes the defect. Several studies revealed that T helper 17 (Th17) cells play a crucial role in pathogenesis, inflammation, and autoimmunity of several autoimmune diseases such as MS. In the present study, we assessed transcript levels of miR-27a and miR-214, in purified CD4(+) T cells of MS patients, during relapsing and remitting phases in inducing differentiation of T na < ve cells to Th17 cells. Forty RR-MS patient samples including those in relapsing (n = 20) and remitting (n = 20) phases were participated in this study. In addition, transcript levels of IL-17A, ROR gamma t, IL-23R, Foxp3, and TGF-beta in purified CD4(+) T cells of patients in relapsing and remitting phases of RRMS patients were compared to healthy controls. Expression levels of miR-27a and miR-214 were measured by RT-qPCR and compared to healthy control group (n = 10). Data indicated upregulation of miR27a in relapsing phase of multiple sclerosis compared to remitting phase and healthy volunteers while miR-214 downregulated in relapsing phase of MS compared to remitting phase and healthy volunteers. In silico studies demonstrated pathways which miR-27a and miR-214 could effect on CD4(+) T cell lineage fate including TGF-beta and mTOR signaling, respectively. Our data suggest that miR-27a may probably inhibit negative regulators of Th17 cell differentiation, thus promoting its differentiation while miR-214 has an adverse effect.
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