Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 5, Issue 19, Pages 9269-9275Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am4020633
Keywords
laser desorption; femtosecond; mass spectrometry; imaging; biofilm; single photon ionization
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Mass spectrometry (MS) imaging is increasingly being applied to probe the interfaces of biomaterials with invasive microbial biofilms, human tissue, or other biological materials. Laser desorption vacuum ultraviolet postionization with similar to 75 fs, 800 nm laser pulses (fs-LDPI-MS) was used to collect MS images of a yeast Escherichia coli co-culture biofilm. The method was also used to depth profile a three-dimensionally structured, multispecies biofilm. Finally, fs-LDPI-MS analyses of yeast biofilms grown under different conditions were compared with LDPI-MS using ultraviolet, nanosecond pulse length laser desorption as well as with fs laser desorption ionization without postionization. Preliminary implications for the use of fs-LDPI-MS for the analysis of biomaterials interfaces are discussed and contrasted with established methods in MS imaging.
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