Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 4, Issue 11, Pages 5907-5914Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am301551n
Keywords
magnetic particles; dextran; immobilization; synthetic affinity ligand; IgG purification
Funding
- Fundacao para a Ciencia e a Tecnologia [PEst-C/EQB/LA0006/2011, PTDC/EBB-BIO/102163/2008, PTDC/EBB-BIO/098961/2008, PTDC/EBB-BIO/118317/2010, SFRH/BD/72650/2010]
- Santander Totta Bank - Universidade Nova de Lisboa
- Fundação para a Ciência e a Tecnologia [PTDC/EBB-BIO/102163/2008, SFRH/BD/72650/2010, PTDC/EBB-BIO/098961/2008] Funding Source: FCT
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Dextran-coated iron oxide magnetic particles modified with ligand 22/8, a protein A mimetic ligand, were prepared and assessed for IgG purification. Dextran was chosen as the agent to modify the surface of magnetic particles by presenting a negligible level of nonspecific adsorption. For the functionalization of the particles with the affinity ligand toward antibodies, three methods have been explored. The optimum coupling method yielded a theoretical maximum capacity for human IgG calculated as 568 +/- 33 mg/g and a binding affinity constant of 7.7 x 10(4) M-1.Regeneration, recycle and reuse of particles was also highly successful for five cycles with minor loss of capacity. Moreover, this support presented specificity and effectiveness for IgG adsorption and elution at pH 11 directly from crude extracts with a final purity of 95% in the eluted fraction.
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