Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 4, Issue 1, Pages 411-417Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am2014713
Keywords
surface modification; silane coupling reagent; glycopolymer; lectin; QCM; membrane
Funding
- [20106003]
- Grants-in-Aid for Scientific Research [24655157, 23685027, 20106003] Funding Source: KAKEN
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A copolymer with a-D-mannose (Man) and trimethoxysilane (TMS) units was synthesized for immobilization on siliceous matrices such as a sensor cell and membrane. Immobilization of the trimethoxysilane-containing copolymer on the matrices was readily performed by incubation at high heat. The recognition of lectin by poly(Man-r-TMS) was evaluated by measurement with a quartz crystal microbalance (QCM) and adsorption on an affinity membrane, QCM results showed that the mannose-binding protein, concanavalin A, was specifically bound on a poly(Man-r-TMS)-immobilized cell with a higher binding constant than bovine serum albumin. The amount of concanavalin A adsorbed during permeation through a poly(Man-r-TMS)immobilized membrane was higher than that through an unmodified membrane. Moreover, the concanavalin A adsorbed onto the poly(Man-r-TMS)-immobilized membrane was recoverable by permeation of a mannose derivative at high concentration.
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