Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 3, Issue 9, Pages 3496-3500Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am200716x
Keywords
nonspecific protein adsorption; Tween 20; immunoassay; liquid crystals; interference colors
Funding
- National University of Singapore
- Agency for Science, Technology and Research (A*STAR) [082 101 0027]
Ask authors/readers for more resources
In this paper, we report the role of surfactants in minimizing nonspecific protein adsorption in liquid crystal (LC)-based immunoassays in which LC is used as a readout system. Among all surfactants tested, only nonionic surfactant such as Tween 20 can effectively reduce the nonspecific protein adsorption, while maintaining the selectivity of the LC-based immunoassay. We also show that to minimize nonspecific protein adsorption, Tween 20 can be added directly into the antibody solution to a final concentration of 0.8 mM. After the addition of Tween 20, better correlations between the antibody concentrations and the interference colors of LCs can therefore be obtained. For example, when Cy3 antibiotin was used, black, yellow, red, and green interference colors correspond to a concentration of 5, 25, 50, and 100 mu g/mL, respectively. This feature gives LC immunoassay a unique advantage over the fluorescence-based immunoassay.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available