Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 3, Issue 1, Pages 50-57Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am100849f
Keywords
protein microarray; microparticle-based assay; hydrogel; agarose; microchannel
Funding
- ETH Zurich
- National Sciences and Engineering Research Council of Canada
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We present a simple, inexpensive, and sensitive technique for producing multiple copies of a hydrogel-based protein microarray. An agarose block containing 25 biofunctionalized channels is sliced perpendicularly to produce many identical biochips. Each microarray consists of 500 mu m spots, which contain protein-coated microparticles physically trapped in porous SeaPrep agarose. Proteins diffuse readily through SeaPrep agarose while the larger microparticles are immobilized in the hydrogel matrix Without major assay optimization the limit of detection is 12 pM for a sandwich assay detecting human IgG. These highly flexible, multiplexed arrays can produced rapidly without any special instrumentation and are compatible with standard fluorescence-based read-out.
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