Article
Biochemical Research Methods
Ashwini Shinde, Pallavi Shinde, Srabani Kar, Kavitha Illath, Souvik Dey, Nitish R. R. Mahapatra, Moeto Nagai, Tuhin Subhra Santra
Summary: In this study, a highly efficient and uniform method for delivering biomolecules into diverse cell types was reported using a titanium micro-ring device activated by infrared light pulse. The device generates photothermal cavitation bubbles, disrupting the cell plasma membrane and allowing biomolecules to enter the cells through diffusion. The successful delivery of various biomolecules into different cell types suggests potential applications in cellular diagnostics and therapeutics.
Article
Engineering, Environmental
Xiu Han, Yan Lu, Haiping Wu, Xiaoxiang Guan, Xueping Ma, Yanan Chu, Bingjie Zou, Guohua Zhou
Summary: In this study, a prism oligonucleotide nanoparticle (pONP)-based delivery system was designed for the specific release of siRNA into telomerase-positive cancer cells. Both in vitro and in vivo tests confirmed the high precision and effectiveness of this nanoparticle cage in delivering siRNA. This delivery method shows great promise for targeted siRNA release into cancer cells.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Yao Jiang, Huimin Zhou, Wenjing Zhao, Shusheng Zhang
Summary: This study presents a self-assembled three-dimensional (3D) DNA nanostructure drug delivery system that utilizes ATP-triggered drug release for tumor fluorescence imaging and targeted therapy. The Dox@3D DNA nanostructures are assembled through a simple annealing reaction and can release drugs rapidly in tumor cells induced by high concentrations of ATP, providing efficient accumulation of drugs in tumors and minimizing damage to normal tissues.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Yue Zhang, Gui Zhao, Jing Liu, Hou-Bing Zhang, Yue Wang, Ying-Li Luo, Zi-Dong Lu, Xiao-Jiao Du, Song Shen, Jin-Zhi Du, Cong-Fei Xu, Jun Wang
Summary: The study proposes a CRISPR-based transcriptional activation nanomedicine that can activate the expression of T cell chemokines and promote T cell migration into solid tumors. Intratumoral or systemic injection of the nanomedicine inhibits tumor growth and improves the efficacy of immunotherapies.
Review
Nanoscience & Nanotechnology
Bappa Maiti, Santanu Bhattacharya
Summary: Natural lipid molecules play a vital role in life, mainly contributing hydrophobicity through alkyl chains. Steroids and isoprenoids are important natural lipid molecules. Synthetic functionalized liposomal nanoparticles can be used for delivering genetic materials.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2022)
Article
Chemistry, Applied
Laura C. Crapina, Sang W. Han, Marcos A. Bizeto
Summary: Efforts are being made to develop gene therapy delivery systems using inorganic nanoparticles such as mesoporous silica for safer and more efficient delivery. In this study, mesoporous silica nanoparticles were functionalized with different alkyl-amino groups and conjugated with plasmid vector pEGFP-N3. The conjugates showed acceptable cytotoxicity to HeLa cells, but low transfection efficiency, possibly due to aggregation in the cell culture medium.
MICROPOROUS AND MESOPOROUS MATERIALS
(2022)
Review
Chemistry, Multidisciplinary
Gary W. Liu, Edward B. Guzman, Nandita Menon, Robert S. Langer
Summary: Endothelial cells have crucial functions in maintaining circulatory homeostasis and serving as the gateway to major organs. Modulating gene expression within these cells can be strategically therapeutic for chronic diseases. Lipid nanoparticles have emerged as potent vehicles for gene delivery to endothelial cells, with potential applications in clinical trials and FDA approval.
PHARMACEUTICAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Elana Ben-Akiva, Johan Karlsson, Shayan Hemmati, Hongzhe Yu, Stephany Y. Tzeng, Drew M. Pardoll, Jordan J. Green
Summary: Nanoparticle-based mRNA cancer vaccines show great promise for personalized cancer treatments. We developed a bioreducible lipophilic poly(beta-amino ester) nanocarrier that can efficiently deliver multiple antigen-encoding mRNAs and nucleic acid-based adjuvants to dendritic cells. The engineered nanocarrier facilitated targeted delivery without the need for surface functionalization and resulted in robust CD8+ T cell responses and efficient antitumor therapy in murine melanoma and colon adenocarcinoma models.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Engineering, Biomedical
Yu Xia, Guoyi Tang, Yi Chen, Changbing Wang, Min Guo, Tiantian Xu, Mingqi Zhao, Yongjian Zhou
Summary: RNA interference (RNAi) is a promising method for treating malignant tumors, and in this study, RGDfC-SeNPs were successfully synthesized as a tumor-targeted carrier for delivering siSox2 to HepG2 liver cancer cells. The results showed that RGDfC-Se@siSox2 effectively silenced Sox2, inhibited tumor cell proliferation, migration, and invasion, induced apoptosis through reactive oxygen species production and mitochondrial membrane potential disruption, and significantly inhibited tumor growth in mice bearing HepG2 tumors. These findings suggest that RGDfC-SeNPs could be a valuable gene carrier for specific gene-targeted therapy in HCC.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Applied
Yahui Liu, Peifang Weng, Yanan Liu, Zufang Wu, Lei Wang, Lianliang Liu
Summary: Citrus fruits are rich in bioactive substances such as pectin, which can be extracted and modified using green techniques to improve their functional properties. This paper compares different extraction and modification techniques for pectin and summarizes the recent advances in citrus pectin for micro/nano-delivery systems construction and application, providing a reference for future utilization.
FOOD HYDROCOLLOIDS
(2022)
Article
Multidisciplinary Sciences
Marina A. Tyumentseva, Aleksandr I. Tyumentsev, Vasiliy G. Akimkin
Summary: Efficiency and optimization of current CRISPR/Cas RNP delivery protocols to target cells will be assessed in this study using molecular cloning, protein expression and purification, cell culture, flow cytometry, and cellular imaging techniques. This will be the first extensive comparative study of popular CRISPR/Cas RNP delivery methods to human cell lines and primary cells.
Article
Medicine, Research & Experimental
Youngrong Park, Abraham S. Moses, Ananiya A. Demessie, Prem Singh, Hyelim Lee, Tetiana Korzun, Olena R. Taratula, Adam W. G. Alani, Oleh Taratula
Summary: This study reports on mRNA-loaded polymeric nanoparticles that induce protein expression in the lungs and muscles. The density of polyethylene glycol on the nanoparticle surface should be optimized based on the purpose of the mRNA treatment and the delivery route.
MOLECULAR PHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Idrissa Diallo, Zeinab Husseini, Sara Guellal, Elodie Vion, Jeffrey Ho, Robert A. Kozak, Gary P. Kobinger, Patrick Provost
Summary: This study characterized the miRNA transcriptome of human liver cells infected with different variants of Ebola virus and identified two viral miRNAs, miR-MAY-251 and miR-MAK-403. These miRNAs have potential regulatory effects on host genes involved in the hemorrhagic phenotype, regulation of viral replication, and modulation of host immune defense.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Ken Cham-Fai Leung, Kathy W. Y. Sham, Josie M. Y. Lai, Yi-Xiang J. Wang, Chi-Hin Wong, Christopher H. K. Cheng
Summary: The study optimized the gene delivery efficiency of ternary composites in transfected glioblastoma cells by tuning the bPEI/citrate-NP ratios. Among the composites prepared, the 0.2ng bPEI/0.5μg pDNA/1.0μg citrate-NP ternary composite showed the best cellular uptake efficiency, while the composite comprising 0.1ng bPEI/0.5μg pDNA/0.1μg citrate-NP exhibited the optimal efficiency for plasmid DNA uptake into the nucleus.
Review
Biochemistry & Molecular Biology
Michael Gabel, Annkathrin Knauss, Dagmar Fischer, Markus F. Neurath, Benno Weigmann
Summary: The RNA interference mechanism may represent a breakthrough in the therapy of diseases caused by gene defects or requiring the inhibition of specific genes. The limitations of siRNA can be overcome by further developments in nanoparticulate drug delivery. Surface-modified siRNA nanoparticles hold promise for more efficient and safer targeting of specific tissues, but developing such delivery strategies remains a major challenge.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chopaka Thongbamrer, Wanlapa Roobsoong, Jetsumon Sattabongkot, Praneet Opanasopit, Boon-ek Yingyongnarongkul
Summary: In this study, a series of L-shaped spermine-based cationic lipids with different hydrophobic chain lengths were designed and synthesized. The lipids with nonidentical hydrocarbon chains showed higher transfection efficiency compared to the identical analogues, and exhibited good transfection performance in different cell lines.
Review
Pharmacology & Pharmacy
Supusson Pengnam, Samarwadee Plianwong, Boon-ek Yingyongnarongkul, Prasopchai Patrojanasophon, Praneet Opanasopit
Summary: siRNA therapy can increase the deposition of chemotherapeutic drugs at the tumor site by suppressing the expression of multidrug-resistant genes. Nonviral delivery systems such as nanovesicles can improve siRNA delivery and have shown great potential in cancer therapy.
DRUG METABOLISM AND PHARMACOKINETICS
(2022)
Article
Engineering, Biomedical
Wipada Samprasit, Praneet Opanasopit, Benchawan Chamsai
Summary: The study developed dual-drugs-loaded nanoparticles for colon-specific delivery and synergistic activity against colon cancer cells. The nanoparticles showed enhanced activity and efficiency when both drugs were loaded, indicating potential for targeted nanomedicines in colon cancer treatment.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Pharmacology & Pharmacy
Taraswi Mitra Ghosh, Teeratas Kansom, Suman Mazumder, Joshua Davis, Ahmed S. Alnaim, Shanese L. Jasper, Chu Zhang, Aedan Bird, Praneet Opanasopit, Amit K. Mitra, Robert D. Arnold
Summary: The study found that the andrographolide analog 3A.1 has anti-tumor activity against mCRPC, either alone or in combination with taxanes, showing synergistic effects. Co-treatment of 3A.1 with taxanes reduced the required dose and regulated key factors such as heat shock proteins and MAT2A to exert its effects.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2022)
Article
Pharmacology & Pharmacy
Theerasak Rojanarata, Kittithat Maithongdee, Nattapong Yuwansri, Sirada Kaewprasert, Thana Thanayutsiri, Noppharat Phadungcharoen, Akhayachatra Chinsriwongkul
Summary: The limit test for determining aluminum levels in citric acid has been found to be dubious and may lead to underestimation of aluminum levels and inaccurate conclusions. The acidity and metal-chelating capabilities of citric acid interfere with the test.
Article
Pharmacology & Pharmacy
Yin Yin Myat, Tanasait Ngawhirunpat, Theerasak Rojanarata, Praneet Opanasopit, Mark Bradley, Prasopchai Patrojanasophon, Chaiyakarn Pornpitchanarong
Summary: This study synthesized PEGDA/AA nanoparticles for the delivery of Dox to colorectal cancer cells. The nanoparticles demonstrated good physical properties, drug loading and release capabilities, non-toxicity to intestinal epithelial cells, and effective killing of colorectal cancer cells. These nanoparticles show potential as a drug delivery carrier for targeted, staged-release therapy for colon cancer.
Article
Pharmacology & Pharmacy
Phuvamin Suriyaamporn, Praneet Opanasopit, Worranan Rangsimawong, Tanasait Ngawhirunpat
Summary: This study designed and developed optimal two-layered dissolving microneedles (MNs) loaded with fluconazole-microemulsions (MEs) for the potential treatment of fungal eye infection. The optimal formulation of MNs exhibited good physical properties, complete insertion, minimally invasive ocular tissue, and high stability, while showing significantly higher FLUZ permeation into the ocular tissue and providing potential antifungal activity.
Article
Pharmacology & Pharmacy
Kwanputtha Arunprasert, Chaiyakarn Pornpitchanarong, Theerasak Rojanarata, Tanasait Ngawhirunpat, Praneet Opanasopit, Prasopchai Patrojanasophon
Summary: This research aimed to develop new hydrophilic drug-in-adhesive patches for transdermal drug delivery. The patches containing PHI-cat and HA showed considerably greater adhesion ability to human skin compared with the patches without catechol and commercial patches. Furthermore, these patches exhibited good physical and chemical stability, making them potential transdermal drug delivery systems with excellent adhesive properties for drug delivery through the skin.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Thirapit Subongkot, Natthan Charernsriwilaiwat, Rattathammanoon Chanasongkram, Kantawat Rittem, Tanasait Ngawhirunpat, Praneet Opanasopit
Summary: This study aimed to develop microemulsions using poloxamer 124 as a surfactant to improve the skin penetration of finasteride. The microemulsions were found to significantly increase the skin penetration of finasteride, mainly through the hair follicle pathway.
Article
Pharmacology & Pharmacy
Kanokwan Singpanna, Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon, Theerasak Rojanarata, Tanasait Ngawhirunpat, S. Kevin Li, Praneet Opanasopit
Summary: The present study evaluated the effect of gold nanoparticles stabilized by chitosan (CS-AuNPs) and citrate ions (Ci-AuNPs) on skin permeation of sodium fluorescein (NaFI) and rhodamine b base (RhB). CS-AuNPs showed enhanced permeation of NaFI, superior to Ci-AuNPs, and this enhancement was likely due to improved delivery through the transepidermal pathway. However, CS-AuNPs and Ci-AuNPs had no significant effect on the permeability of the lipophilic molecule RhB. CS-AuNPs also exhibited no cytotoxicity towards human skin fibroblast cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Thapakorn Charoenying, Praneet Opanasopit, Tanasait Ngawhirunpat, Theerasak Rojanarata, Prasert Akkaramongkolporn, Prasopchai Patrojanasophon
Summary: This study aimed to use a 3D printer to fabricate a tablet-shaped floating device for controlling the release and floating time of domperidone tablets. The device was designed with a PVA pathway to provide a predictable floating time, and the air chamber was intentionally detached to allow elimination from the body. The optimal formulation achieved a floating time of 24 hours and a controlled drug release rate.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Benchawan Chamsai, Praneet Opanasopit, Wipada Samprasit
Summary: This study developed fast disintegrating dosage forms for oral insulin delivery by incorporating mucoadhesive thiolated chitosan (TCS)-based nano-particles (NPs). The results showed that the CD-insulin complexes were successfully encapsulated into the mucoadhesive NPs, which were promising for use in oral insulin delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Phuvamin Suriyaamporn, Chaiyakarn Pornpitchanarong, Boonnada Pamornpathomkul, Prasopchai Patrojanasophon, Theerasak Rojanarata, Praneet Opanasopit, Tanasait Ngawhirunpat
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Supusson Pengnam, Praneet Opanasopit, Theerasak Rojanarata, Boon-ek Yingyongnarongkul, Chopaka Thongbamrer, Samarwadee Plianwong
Summary: The challenge in HER2-overexpressing breast cancer therapy is to develop an effective target therapy. This study formulated lipid-based nanoparticles for delivering siRNA into HER2-overexpressing breast cancer cells. The dual-targeted therapy using Mcl-1 siRNA nioplex and trastuzumab showed synergistic effects in inhibiting cell growth and inducing apoptosis.
Review
Pharmacology & Pharmacy
Kanokwan Singpanna, Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon, Theerasak Rojanarata, Tanasait Ngawhirunpat, S. Kevin Li, Praneet Opanasopit
Summary: Transdermal drug delivery via the skin is a promising method, and gold nanoparticles (AuNPs) have emerged as a useful tool in this area. This review provides a comprehensive summary of the properties, synthesis, and applications of AuNPs in transdermal drug delivery.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
