Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Jianfeng Li, Xiao Liu, Jeremy M. Crook, Gordon G. Wallace
Summary: Tissue engineered constructs can serve as in vitro models for research and replacement of diseased or damaged tissue. 3D bioprinting technology allows for the arrangement of biomaterials and cells to facilitate tissue engineering. Alginate has limited cell affinity and lacks functional groups for cell growth promotion, while graphene oxide demonstrates support for various cell types.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jianhua Zhang, Esther Wehrle, Marina Rubert, Ralph Mueller
Summary: Three-dimensional (3D) bioprinting is a promising approach for tissue engineering, allowing precise placement of cells and biomaterials to mimic complex microstructures of biological tissues. Bioprinted tissues can benefit from exposure to fluid mechanical cues provided by bioreactor systems, aiding in tissue development and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Yongcong Fang, Mengke Ji, Yi Yang, Yihan Guo, Ruobin Sun, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: Three-dimensional bioprinting has been hindered by a lack of suitable bioinks for printing functional tissues. We developed a granular cell aggregate-based biphasic (GCAB) bioink that exhibited desired properties for extrusion bioprinting and modeling the mechanical characteristics of soft tissues. By preorganizing GCAB bioink and printing it with an endothelial cell-laden gelatin bioink, we successfully created functional hepatic tissues with high cell density and a perfusable vascular network. This GCAB bioink design has potential for creating functional tissues for therapeutic applications.
Review
Pharmacology & Pharmacy
Maria Lazaridou, Dimitrios N. Bikiaris, Dimitrios A. Lamprou
Summary: Bioprinting is a promising technology for developing functional tissue constructs and controlled drug delivery systems. Chitosan, a natural polysaccharide, is an attractive bioink for tissue regeneration and restoration.
Article
Engineering, Biomedical
Iris Ianovici, Yedidya Zagury, Idan Redenski, Neta Lavon, Shulamit Levenberg
Summary: Cultivated meat uses tissue engineering concepts to create sustainable and edible muscle tissues to meet the growing demand for meat products. This study evaluated plant-protein-enriched scaffolds as 3D-printable platforms for maturation and cellular printing of bovine satellite cells. The findings show great promise for further research in the field of cultivated meat.
Review
Engineering, Biomedical
Chengcheng Du, Wei Huang, Yiting Lei
Summary: Three-dimensional (3D) bioprinting technology is an advanced technique in tissue engineering and regenerative medicine. Microgels, a new type of bioink, have adjustable pore structures and excellent mechanical and biological properties, making them great candidates for 3D bioprinting applications. This review discusses the application and future development of microgels in biomedicine and 3D bioprinting.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Jiannan Li, Tara Shelby, Hossein Vahid Alizadeh, Hannah Shelby, Yunzhi Peter Yang
Summary: In this study, an automated active mixing platform (AAMP) was developed for high-quality preparation of hydrogel bioinks. AAMP, adapted from syringe pumps, offers advantages such as low cost, automated control, high precision, customizability, great cytocompatibility, and intelligent homogeneity detection. The AAMP demonstrated the capability to achieve fast and homogeneous hydrogel mixing, showing great promise for bioprinting and tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Materials Science, Multidisciplinary
Yi Zhang, Piyush Kumar, Songwei Lv, Di Xiong, Hongbin Zhao, Zhiqiang Cai, Xiubo Zhao
Summary: 3D bioprinting technology combines computing science, biology, and material engineering, and has great potential in fabricating vascularized constructs for tissue engineering. This review summarizes recent advances in 3D bioprinting of vascularized tissues, including the selection of biomaterials and various 3D printing techniques and applications.
MATERIALS & DESIGN
(2021)
Review
Engineering, Biomedical
Suhun Chae, Dong-Woo Cho
Summary: The development of three-dimensional (3D) bioprinting has facilitated the creation of tissue constructs that resemble natural tissues in structure and function. This review focuses on the rational design and biofabrication strategies of 3D bioprinted tissue constructs for orthopedic tissue engineering applications. The review summarizes the fundamentals of 3D bioprinting techniques and biomaterial inks, the recent advances in 3D bioprinting technology for orthopedic tissue engineering, and the challenges and future directions of 3D bioprinting in orthopedic tissue engineering and regenerative medicine.
ACTA BIOMATERIALIA
(2023)
Review
Biotechnology & Applied Microbiology
Haonan Wang, Huaqing Yu, Xia Zhou, Jilong Zhang, Hongrui Zhou, Haitong Hao, Lina Ding, Huiying Li, Yanru Gu, Junchi Ma, Jianfeng Qiu, Depeng Ma
Summary: This article reviews the significance and recent progress of ECM-based biomaterials in 3D bioprinting, as well as their applications in tissue regeneration and cancer research. There is great potential for future development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Federica Potere, Beatrice Belgio, Giorgio Alberto Croci, Silvia Tabano, Paola Petrini, Gabriele Dubini, Federica Boschetti, Sara Mantero
Summary: The study aims to develop a printable bioink that can finely reproduce the complex structure of large blood vessels. Through decellularization and combining different components, successfully printed structures that meet design requirements were achieved. By adjusting printing parameters and the amount of dECM, the desired mechanical properties could be met.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Materials Science, Multidisciplinary
Haonan Wang, Shihao Bi, Bingbing Shi, Junchi Ma, Xiangwei Lv, Jianfeng Qiu, Yunyun Wei
Summary: 3D bioprinting enables the controlled deposition of bioink containing cells to fabricate complex bionic tissue structures in a fast and scalable manner, revolutionizing clinical organ transplantation. Bioprinting has broad applications in tissue engineering, life sciences, and clinical medicine. This review explores the concept, development, and composition of bioink, with a focus on the advantages and disadvantages of various biomaterials, as well as the use of common cells and biomolecules. Additionally, it discusses the properties and applications of stimuli-responsive smart materials for 4D bioprinting and summarizes the challenges and development trends of bioink.
ADVANCED ENGINEERING MATERIALS
(2023)
Review
Engineering, Multidisciplinary
Zizhuo Zheng, David Eglin, Mauro Alini, Geoff R. Richards, Ling Qin, Yuxiao Lai
Summary: Three-dimensional (3D) bioprinting is an emerging technology that assembles biocompatible materials and cells to create tissue engineering solutions. Photopolymerized materials play a crucial role in the success of 3D bioprinting. Visible light is considered more biocompatible than UV light for bioprinting applications.
Review
Pharmacology & Pharmacy
Wenzhuo Fang, Ming Yang, Meng Liu, Yangwang Jin, Yuhui Wang, Ranxing Yang, Ying Wang, Kaile Zhang, Qiang Fu
Summary: The regeneration of biological tissues in medicine is challenging, and 3D bioprinting offers an innovative way to create functional multicellular tissues. However, for clinical application, bioprinting still needs improvement in areas such as vascularization, antibacterial effectiveness, immunomodulation, and collagen deposition regulation.