Article
Biology
Xueyong Wang, Murad Nawaz, Chris DuPont, Jessica H. Myers, Steve Ra Burke, Roger A. Bannister, Brent D. Foy, Andrew A. Voss, Mark M. Rich
Summary: Excitation-contraction coupling is the process of converting electrical excitation of muscle into force generation. Depolarization of skeletal muscle can lead to failure of ECC, specifically the failure to generate calcium transients. Different AP properties, such as AP peak and AP integral, play important roles in different steps of ECC, including AP conduction and calcium release.
Review
Physiology
Pura Bolanos, Juan C. Calderon
Summary: The excitation-contraction coupling in skeletal muscle is a crucial link between membrane excitation and mechanical contraction mediated by Ca2+, involving multiple mechanisms of Ca2+ release and recycling.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dmitry Shishmarev, Emily Rowland, Shouvik Aditya, Srinivasan Sundararaj, Aaron J. Oakley, Angela F. Dulhunty, Marco G. Casarotto
Summary: Excitation-contraction coupling is the process by which an electrical signal is converted into muscle contraction. In this study, the interaction between two calcium channels, dihydropyridine receptor (DHPR) and ryanodine receptor 1 (RyR1), during the excitation-contraction coupling process was investigated. The results showed that the protein STAC3 interacts with a subunit of the DHPR known as the II-III loop, and this interaction differs from the interaction with the neuronal isoform STAC2. These findings provide insights into the molecular mechanism of excitation-contraction coupling in skeletal muscle.
Review
Biochemical Research Methods
Anna Thalacker-Mercer, Jamie Blum
Summary: The global population of individuals over the age of 65 and those at risk of malnutrition is increasing, leading to a rise in diet-related chronic diseases. Effective strategies are urgently needed to improve health outcomes. While nutrient needs have been identified and products developed, there is often a delay in their utilization in clinical practice.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Clara Bernard, Charline Jomard, Benedicte Chazaud, Julien Gondin
Summary: Post-injury skeletal muscle regeneration requires interactions between myogenic and non-myogenic cells. The kinetics of changes in muscle stem cells, endothelial cells, fibro-adipogenic progenitors, and macrophages in the regenerating muscle were similar in mild and severe injury models. However, the magnitude of changes in the number of differentiating muscle stem cells, hematopoietic cells, and fibro-adipogenic progenitors was higher in severe muscle damage.
Review
Physiology
Walter C. Thompson, Paul H. Goldspink
Summary: This review focuses on the crucial role of 14-3-3 proteins in regulating excitation-contraction coupling in muscle, highlighting the need for further investigation into their mechanistic actions in force generation.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Engineering, Biomedical
Emma Lubel, Bruno Grandi Sgambato, Deren Y. Barsakcioglu, Jaime Ibanez, Meng-Xing Tang, Dario Farina
Summary: This study fills the gap in the research on human neuromechanical control at the motor unit level by analyzing the kinematics of muscle units in natural contractions. By combining high-density surface electromyography and ultrafast ultrasound recordings, the motion of individual muscle units during contractions was measured. The study provides quantitative metrics such as the duration of motion, total contraction time, and excitation-contraction coupling delay. Additionally, the study also discovered muscle unit twisting and distinct split regions in muscle unit territories during voluntary contractions.
JOURNAL OF NEURAL ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Irene Pertici, Lorenzo Bongini, Marco Caremani, Massimo Reconditi, Marco Linari, Gabriella Piazzesi, Vincenzo Lombardi, Pasquale Bianco
Summary: The study analyzes the mechanical performances of vertebrate skeletal muscle during isometric and isotonic contractions, and their corresponding energy consumption, to define the coupling between mechanical and biochemical steps in the myosin-actin energy transduction cycle. The emergent properties of a simplified synthetic nanomachine, consisting of eight HMM molecules and an actin filament, are also assessed. The study predicts the relationships between energetic and mechanical properties of fast skeletal muscle, both in vivo and in vitro, through a three-state model of myosin-actin interaction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Kaizhi Ding, Yanchun Gong, Chunfang Cheng, Xiaonuo Li, Yuanjie Zhu, Xiaofei Gao, Yuhua Li, Chunhua Yuan, Zhibing Liu, Wei Jiang, Chong Chen, Li-Hua Yao
Summary: This study investigates the role of voltage-gated sodium channels (VGSC) in muscle regeneration. The findings suggest that with the progression of differentiation, VGSC expression and intracellular sodium concentration increase, and VGSC dynamics change, potentially contributing to muscle contraction.
CELLULAR SIGNALLING
(2024)
Article
Multidisciplinary Sciences
Alexandre Prola, Jordan Blondelle, Aymeline Vandestienne, Jerome Piquereau, Raphael G. P. Denis, Stephane Guyot, Hadrien Chauvin, Arnaud Mourier, Marie Maurer, Celine Henry, Nahed Khadhraoui, Cindy Gallerne, Thibaut Molinie, Guillaume Courtin, Laurent Guillaud, Melanie Gressette, Audrey Solgadi, Florent Dumont, Julien Castel, Julien Ternacle, Jean Demarquoy, Alexandra Malgoyre, Nathalie Koulmann, Genevieve Derumeaux, Marie-France Giraud, Frederic Joubert, Vladimir Veksler, Serge Luquet, Frederic Relaix, Laurent Tiret, Fanny Pilot-Storck
Summary: Mice deficient for a muscle-specific enzyme of very-long-chain fatty acid synthesis displayed increased basal energy expenditure and protection against high-fat diet-induced obesity. Muscle-specific modulation of the very-long-chain fatty acid pathway was associated with a reduced content of inner mitochondrial membrane phospholipid cardiolipin and a blunted coupling efficiency between the respiratory chain and ATP synthase, which was restored by cardiolipin enrichment. Selective increase of lipid oxidative capacities in skeletal muscle, through the cardiolipin-dependent lowering of mitochondrial ATP production, provides an effective option against obesity at the whole-body level.
Article
Biochemistry & Molecular Biology
Chih-Hsuan Chou, Elisabeth R. Barton
Summary: Glucose is a vital energy source for active skeletal muscles, and AMPK plays a crucial role in glucose uptake during muscle contraction. The study aimed to investigate the impact of increased muscle IGF-I on AMPK α phosphorylation and activity. The results demonstrated that high muscle IGF-I enhances glucose uptake under resting conditions but does not inhibit AMPK activity during muscle contraction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Sadegh Davoudi, Bin Xu, Erik Jacques, Jose L. Cadavid, Matthew McFee, Chih-Ying Chin, Amir Meysami, Majid Ebrahimi, Mohsen A. Bakooshli, Kayee Tung, Henry Ahn, Howard J. Ginsberg, Alison P. McGuigan, Penney M. Gilbert
Summary: Stem cell-mediated endogenous repair is often evaluated through animal studies, but this study describes an in vitro assay that mimics important early stages of in vivo skeletal muscle repair. By integrating a semi-automated image analysis pipeline, high-content data analysis of muscle fiber content and morphology is enabled. The study found that the spatiotemporal dynamics of in vitro repair closely matched those observed in vivo only when both stem cells and injury were present. The engineered tissue's imaging geometry allowed for exploration of cellular mechanisms driving the muscle stem cell response to the regenerative template.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Multidisciplinary Sciences
Quinton Banks, Hugo Bibollet, Minerva Contreras, Daniel F. Bennett, Roger A. Bannister, Martin F. Schneider, Erick O. Hernandez-Ochoa
Summary: The study investigates the role of voltage-sensing domains (VSDs) in excitation-contraction coupling (ECC) by encoding cysteine substitutions on each S4 voltage-sensing segment of Ca(V)1.1 and using in cellulo AP fluorometry to track the movement of each VSD in skeletal muscle fibers. The results suggest that VSDs play a crucial role in charge movement and Ca2+ release during skeletal muscle fiber activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Anamika Dayal, Stefano Perni, Clara Franzini-Armstrong, Kurt G. Beam, Manfred Grabner
Summary: The skeletal muscle DHPR beta(1a) subunit is crucial for skeletal muscle EC coupling. All four vertebrate beta isoforms can restore alpha(1S) triad targeting, but only beta(1a) can restore proper arrangement of DHPRs and interaction with RyR1. The distal C terminus of beta(1a) plays a pivotal role in the reconstitution of DHPR tetrads and their interaction with RyR1, essential for skeletal muscle EC coupling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Daniela Rossi, Stefania Lorenzini, Enrico Pierantozzi, Filip Van Petegem, David Osamwonuyi Amadsun, Vincenzo Sorrentino
Summary: Junctin is a transmembrane protein that plays a role in striated muscles by interacting with calsequestrin and the ryanodine receptor. Our study found that junctin and calsequestrin co-localize in the endoplasmic reticulum and triads, and identified specific regions of junctin that can bind calsequestrin and the RyR. Deletion of these regions affects junctin's localization at the junctional SR.
JOURNAL OF CELL SCIENCE
(2022)