Review
Engineering, Biomedical
Kun Liu, Nan Hu, Zhihai Yu, Xinzhou Zhang, Hualin Ma, Huawei Qu, Changshun Ruan
Summary: This article summarizes the development of 3D printing and bioprinting technologies in urology over the past decade. By searching and summarizing the literature, eight common 3D printing technologies and their characteristics are introduced, and the application of 3D printing in urology, as well as the potential applications of 3D bioprinting, are discussed.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Joshua W. Tashman, Daniel J. Shiwarski, Brian Coffin, Alexander Ruesch, Frederick Lanni, Jana M. Kainerstorfer, Adam W. Feinberg
Summary: As 3D bioprinting continues to advance as a fabrication technology, there is a growing need for better analytical methods to characterize engineered constructs. This study presents a multimaterial bioprinting platform with integrated optical coherence tomography for in situ volumetric imaging, error detection, and 3D reconstruction. By improving the collagen bioink compositions, gelatin microparticle support bath optical clearing, and optimizing the machine pathing, the quality assessment of bioprinted constructs is enhanced.
Article
Biotechnology & Applied Microbiology
Marta Merli, Lorenzo Sardelli, Nicolo Baranzini, Annalisa Grimaldi, Emanuela Jacchetti, Manuela Teresa Raimondi, Francesco Briatico-Vangosa, Paola Petrini, Marta Tunesi
Summary: This study proposes a highly controllable method to optimize the printability of internally crosslinked polysaccharides by introducing pH as a key parameter. The results indicate that pectin-based hydrogels could be successfully applied for neural cell culture.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Fatima Garcia-Villen, Amaia Guembe, Jose M. Rey, Teresa Zuniga, Sandra Ruiz-Alonso, Laura Saenz-del-Burgo, Jesus M. Izco, Jose I. Recalde, Jose Luis Pedraz
Summary: Collagen is a key protein for tissue engineering and 3D bioprinting, but it comes with challenges such as complex extraction processes and batch-to-batch reproducibility issues. This study evaluates the use of new, fibrillar type I collagen as a standardized and reproducible collagen source for 3D printing and bioprinting. The native, type I collagen demonstrated excellent performance during 3D printing and enabled cell inclusion without hindering printability.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Review
Chemistry, Organic
Luke O. Jones, Leah Williams, Tasmin Boam, Martin Kalmet, Chidubem Oguike, Fiona L. Hatton
Summary: Cryogels are macroporous polymeric structures formed by cryogelation of monomers/polymer in a solvent below freezing temperature. They are increasingly being used in biomedical applications such as 3D bioprinting, drug delivery, wound healing, and injectable therapeutics due to their interconnected macroporosity, ease of preparation, and biocompatibility. Recent studies have shown promising results in using cryogels for cancer treatment, surgical implantation, and wound dressings.
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Polymer Science
Rory Gibney, Jennifer Patterson, Eleonora Ferraris
Summary: Research has successfully demonstrated the production of bioprinted recombinant collagen films suitable for corneal tissue engineering using aerosol jet printing technology, showing favorable mechanical and optical properties.
Review
Chemistry, Multidisciplinary
Zhen Wang, Lei Xiang, Feng Lin, Yunkai Tang, Wenguo Cui
Summary: This review provides an overview of 3D bioprinting and the main strategies used for homeostasis bioprinting. It introduces the principles of 3D bioprinting system applied in homeostasis regulations and summarizes the specific strategies and potential trends using stimuli-response biomaterials. The recent breakthrough in vasculature fabrication shows a promising prospect in hormone regulation. Challenges and future prospects of homeostasis fabrication based on 3D bioprinting in regenerative medicine are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Cyrus W. Beh, Dionis S. Yew, Ruth J. Chai, Sau Yin Chin, Yiqi Seow, Shawn S. Hoon
Summary: The study introduces a hydrogel 3D printer that can print complex structures quickly and continuously, and is capable of multi-material printing. This innovative approach helps address the nutrient supply issue in bioprinting.
Article
Biochemistry & Molecular Biology
Hsu-Hsiang Lin, Pen-Hsiu Grace Chao, Wei-Chiu Tai, Po-Chun Chang
Summary: This study successfully developed a biomimetic microfibrous system to assist PDL regeneration and withstand functional load. Collagen-based microfibers were fabricated and PDL cells seeded on waveform microfibers showed enhanced growth and gene expression levels, indicating potential for promoting healing and regeneration under shear stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Materials Science, Multidisciplinary
Joseph D. Tanfani, Jasmin D. Monpara, Sriramakamal Jonnalagadda
Summary: 3D printing is widely used by independent researchers in various fields, with 3D bioprinting being a specific form that utilizes bioinks to create complex, bioactive structures such as artificial skin for wound regeneration. Despite the wide range of available materials and cell types, an ideal bioink for printing artificial skin has yet to be found. This review will discuss the different types of 3D printing, commonly used bioinks, challenges in 3D bioprinting of skin, and future directions for the field.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Review
Polymer Science
Quinten Thijssen, Joseph Toombs, Chi Chung Li, Hayden Taylor, Sandra Van Vlieberghe
Summary: This review comprehensively discusses the potential of volumetric 3D-printing, summarizing the theoretical framework of photopolymerization and highlighting the advancements in light-based 3D-printing techniques. Special attention is given to the subfield of volumetric bioprinting. The review provides important discussions and prospects for those interested in the field of volumetric 3D-printing.
PROGRESS IN POLYMER SCIENCE
(2023)
Review
Engineering, Biomedical
Yuhang Zhang, Liuxiang Chen, Bing Hu
Summary: Compared to orthopedics, the application of 3D printing in the gastrointestinal system is still less developed. Current clinical applications mainly focus on preoperative planning, surgical guidance, and education. Promising results have been achieved, but there is a lack of reasonable study design and proper comparisons. Key requirements in clinical scenarios include structural reconstruction, replacement, defect repair, drug screening, and delivery. Most studies have been conducted on small animal or in vitro models, and achieving prints that are compatible with human size, histology, and functions still requires further progress.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Engineering, Biomedical
Peng Zhang, Haoxuan Wang, Peng Wang, Yating Zheng, Linxiang Liu, Jun Hu, Yande Liu, Qing Gao, Yong He
Summary: This study presents a lightweight stereolithography-based 3D bioprinting system with smart mechanical and structural design, suitable for printing mixed cells with good biocompatibility. The system is compact, low-cost, and equipped with a mini bioink chamber, making it ideal for 3D bioprinting.
BIOACTIVE MATERIALS
(2021)
Article
Materials Science, Multidisciplinary
Pei Zhuang, Zachary Greenberg, Mei He
Summary: A novel 3D printable nanocomposite starch hydrogel was developed with enhanced biocompatibility for promoting 3D cell growth, utilizing gelatin nanoparticles and collagen. The unique rheological properties of the hydrogel, along with the homogeneous microporous structure containing collagen fibers and gelatin nanoparticles, support cell attachment and proliferation. The cell metabolic activity study confirmed the remarkable enhancement of biological function of the developed starch hydrogel, making it a highly desirable bio-ink for advancing 3D tissue engineering.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Chemistry, Multidisciplinary
Wanlu Li, Mian Wang, Luis Santiago Mille, Juan Antonio Robledo Lara, Valentin Huerta, Tlalli Uribe Velazquez, Feng Cheng, Hongbin Li, Jiaxing Gong, Terry Ching, Caroline A. Murphy, Ami Lesha, Shabir Hassan, Tim B. F. Woodfield, Khoon S. Lim, Yu Shrike Zhang
Summary: A portable 3D Digital Light Processing (DLP) printer has been developed using a smartphone-powered projector and custom-written app, enabling the printing of detailed structures and tissue analogs. The printer allows for fabrication of medical implants and cell-laden tissues on-site, marking a milestone in mobile-health technologies. The integration of a 3D scanning smartphone app further enhances the printer's capabilities for various applications in resource-limited and point-of-care settings.
ADVANCED MATERIALS
(2021)
Article
Engineering, Biomedical
Aleksandra Filippova, Fabien Bonini, Liudmila Efremova, Manon Locatelli, Olivier Preynat-Seauve, Amelie Beduer, Karl-Heinz Krause, Thomas Braschler
Summary: This study demonstrates the usage of elastic macroporous cryogels for differentiation and transplantation of mature neurons, with a focus on covalent immobilization of neural adhesion proteins for long-term neuronal culture. The research shows compatibility with dopaminergic differentiation of LUHMES and hESCs in the context of cell therapy for Parkinson's disease, and suggests the potential of the Neurothread approach in providing more mature and less proliferative cells in vivo.
Article
Mechanics
Alessandro Bongarzone, Arnaud Bertsch, Philippe Renaud, Francois Gallaire
Summary: The study investigates self-sustained oscillations induced by the interaction of two impinging jets in microfluidic devices, confirming a predominantly two-dimensional physical mechanism. Utilizing global stability analysis and weakly nonlinear theory, the research reveals hysteresis in the system within a certain aspect ratio range. The Kelvin-Helmholtz instability is identified as the main candidate for the observed oscillations in fluidic devices.
JOURNAL OF FLUID MECHANICS
(2021)
Article
Engineering, Biomedical
Ludovic Serex, Kunal Sharma, Victor Rizov, Arnaud Bertsch, John D. McKinney, Philippe Renaud
Summary: Despite its simplicity, extrusion-based bioprinting has limitations in reproducing complex tissue architecture found in organs due to the need for lower cell concentrations to avoid cell lysis and loss. A microfluidic-based print head allows real-time modulation of printed cell concentration, enabling bioprinting at high cell concentrations for more reliable and reproducible results in organoid printing.
Article
Chemistry, Multidisciplinary
Amelie Beduer, Fabien Bonini, Connor A. Verheyen, Martina Genta, Mariana Martins, Joe Brefie-Guth, Josefine Tratwal, Aleksandra Filippova, Patrick Burch, Olaia Naveiras, Thomas Braschler
Summary: A novel type of injectable biomaterial with elastic softening transition properties has been developed, allowing in vivo shaping and induction of stable 3D vascularized tissue. Through extensive numerical simulation, this injectable meta-biomaterial can reversibly change stiffness during deformation, potentially providing new perspectives in soft tissue engineering and regenerative medicine.
ADVANCED MATERIALS
(2021)
Article
Engineering, Biomedical
Amelie Beduer, Martina Genta, Nicolas Kunz, Connor Verheyen, Mariana Martins, Joe Brefie-Guth, Thomas Braschler
Summary: Soft tissue reconstruction is a challenging field, and this study analyzes the performance of an elastic porous injectable biomaterial for volume retention and tissue colonization. The material shows nearly quantitative volumetric stability and effective colonization with autologous fibrovascular tissue. Comparative analysis with clinical reference materials provides important design rules for volume stability and tissue induction in soft tissue regeneration.
ACTA BIOMATERIALIA
(2022)
Article
Cell Biology
Sanae El Harane, Stephane Durual, Thomas Braschler, Dominik Andre-Levigne, Nicolo Brembilla, Karl-Heinz Krause, Ali Modarressi, Olivier Preynat-Seauve
Summary: This study developed a novel method to produce homogeneous adipose-derived stem cell (ASC) spheroids and demonstrated their efficacy in improving the stability of fat transplants. The ASC spheroids maintained regenerative gene expression and had an enriched secreted proteome compared to single-cell suspensions of ASC. In an animal model, the addition of ASC spheroids to fat grafts resulted in better long-term stability compared to single ASC suspension.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Bartosz Gerard Gdaniec, Fabien Bonini, Francois Prodon, Thomas Braschler, Thilo Kohler, Christian van Delden
Summary: RLPs produced by Pseudomonas aeruginosa form micelles and deliver self-produced toxic compounds to Staphylococcus aureus, enhancing its killing effect and also potentiating the activity of lincosamide antibiotics.
Article
Neurosciences
Fabien Bonini, Sebastien Mosser, Flavio Maurizio Mor, Anissa Boutabla, Patrick Burch, Amelie Beduer, Adrien Roux, Thomas Braschler
Summary: Recent advances in biomaterials offer new possibilities for brain tissue reconstruction, and interstitial fluid pressure plays a significant role in the integration of porous biomaterials.
Article
Chemistry, Analytical
Joan Teixidor, Salvatore Novello, Daniel Ortiz, Laure Menin, Hilal A. Lashuel, Arnaud Bertsch, Philippe Renaud
Summary: The paper introduces a new Droplet on Demand (DoD) sampling method for collecting molecules from brain extracellular fluid in mice, allowing fast and accurate glucose quantification without transient regimes. This technique has the potential to be valuable for research on neurodegenerative diseases.
ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Clementine Lipp, Laure Koebel, Arnaud Bertsch, Michael Gauthier, Aude Bolopion, Philippe Renaud
Summary: We present a microfluidic dielectrophoretic-actuated system for trapping single cells and forming controlled cell aggregates. We propose a novel method to characterize the efficiency of dielectrophoretic trapping, taking into account both flow speed and heat generated by the traps as limiting factors for safe cell manipulation. Experimental comparison of two original designs with different manufacturing processes identifies the most efficient design, which is validated by monitoring cell membrane integrity using fluorescence imaging. We suggest design rules for adapting the traps to multiple-cell trapping, and experimentally demonstrate the formation of controlled size and composition aggregates using two different cell types. This study provides a simple manufactured tool for the controlled manipulation of particles in the composition of multicellular assemblies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fatemeh Navaee, Philippe Renaud, Alexander Kleger, Thomas Braschler
Summary: Biochemical and biophysical properties of cardiac tissue can be manipulated by using a blend of decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen, which has shown enhanced cardiogenic differentiation and beating motion in a 3D cardiac cell culture model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Fatemeh Navaee, Philippe Renaud, Niccolo Piacentini, Mathilde Durand, Dara Zaman Bayat, Diane Ledroit, Sarah Heub, Stephanie Boder-Pasche, Alexander Kleger, Thomas Braschler, Gilles Weder
Summary: Myocardium is composed of cardiac cells that interact with their environment and their structure affects the physiology, function, and metabolism of cardiac tissue. This study aims to understand the mechanism of generating off-axis alignments in the myocardium and develop a reliable in vitro cardiac model. By applying cyclic mechanical stimulation and groove constraint, the researchers were able to engineer off-axis alignments similar to the helical arrangements observed in vivo. The study also shows that cyclical stretching supports cell survival and development, improving the model's function.
BIOENGINEERING-BASEL
(2023)
Article
Biochemical Research Methods
Jiande Zhou, Amaury Wei, Arnaud Bertsch, Philippe Renaud
Summary: The Poisson limit poses a major challenge for isolating single cells, but this article presents a passive microfluidic system that overcomes this limitation with high efficiency. It has a high throughput and low fault ratio, making it suitable for various cells and particles.
Article
Biochemical Research Methods
Albert Leroy, Joan Teixidor, Arnaud Bertsch, Philippe Renaud
Summary: This study successfully quantified dopamine concentrations in droplets using electrochemical techniques on PDMS-fused silica chips prepared with a simple fabrication technique. The unprecedented measurements at low concentrations in T-junction microfluidic chips showed a detection limit of 207 nM, comparable to continuous flow electrochemistry results, demonstrating the usefulness of the method for quantifying microsampled chemicals in droplets.
Article
Engineering, Biomedical
Zahra Sadat Ghazali, Mahnaz Eskandari, Shahin Bonakdar, Philippe Renaud, Omid Mashinchian, Shahriar Shalileh, Fabien Bonini, Ilker Uckay, Olivier Preynat-Seauve, Thomas Braschler
Summary: Cell-imprinting technology is a novel method that directs stem cell fate by using substrates molded from target cells. The study demonstrated that cell-imprinted substrates can accelerate neural differentiation and induce morphological changes in stem cells, leading to upregulation of neural stem cell and early neuronal markers.
