Article
Chemistry, Analytical
Peixian Li, Ye Ai
Summary: Biophysical markers of cells have shown promise in studying, characterizing, and classifying different cell types. The lack of cell sorting ability based on multivariate biophysical markers presents a challenge in isolating specific cell types. This study demonstrates label-free and high-throughput acoustic single-cell sorting activated by the characterization of multivariate biophysical phenotypes, providing a solution to the challenges of cell purification without specific molecular biomarkers.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Hossein Tavassoli, Prunella Rorimpandey, Young Chan Kang, Michael Carnell, Chris Brownlee, John E. Pimanda, Peggy P. Y. Chan, Vashe Chandrakanthan
Summary: A label-free method using inertial microfluidics is developed to purify viable cardiomyocytes from mouse neonatal hearts with purities over 90%. The isolated cardiomyocytes retain their identity and function, exhibiting different physico-mechanical properties compared to non-cardiomyocytes. This method could be a valuable tool for advancing the understanding of cardiomyocyte identity and function, with potential benefits for cell therapy and diagnostic applications.
Review
Chemistry, Analytical
Yao Chen, Zheng Zhou, Shu Zhu, Zhonghua Ni, Nan Xiang
Summary: The rapid development of microfluidic technologies has provided a promising tool for single-cell analysis, enabling a deeper understanding of the physiological and pathological states of cells. This review provides a comprehensive overview of the recent advances in label-free microfluidics for analyzing the biophysical properties of single cells, and discusses their applications in cell identification, disease diagnosis, and drug resistance studies.
MICROCHEMICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Muhammad Tayyab, Pengfei Xie, Muhammad Ahsan Sami, Hassan Raji, Zhongtian Lin, Zhuolun Meng, Seyed Reza Mahmoodi, Mehdi Javanmard
Summary: The article introduces a novel electronic interface for portable protein detection and demonstrates its reliability and sensitivity. The aim of the research is to explore the potential of using impedance sensing as a method for protein detection and achieve personalized health monitoring.
SCIENTIFIC REPORTS
(2022)
Article
Nanoscience & Nanotechnology
Yufang Hu, Zhicheng Huang, Biwu Liu, Juewen Liu
Summary: The study focused on the interaction between Hg(II) and AuNPs, showing that Hg(II) affected the color change of AuNPs and promoted DNA adsorption, indicating that the interaction between Hg(II) and AuNPs was the dominant factor. It is important to consider target and AuNP interactions when designing label-free sensors.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Analytical
Bingyao Shi, Xiao Chen, Yuanyuan Cai, Shuai Zhang, Tao Wang, Yiquan Wang
Summary: A novel all-pass slot microring resonator (SMRR) for label-free optical biosensing is proposed in this paper. Numerical calculations show that the sensor has high concentration and refractive index sensitivity, as well as a low detection limit. The SMRR is an excellent candidate for label-free optical biosensors due to its compact structure and excellent sensing capability.
Article
Biophysics
Junchen Liao, Jifeng Ren, Huang Wei, Raymond H. W. Lam, Song Lin Chua, Bee Luan Khoo
Summary: Current methods for detecting phagocytosis pose significant challenges, necessitating the development of a rapid, non-disruptive, and label-free detection approach. By using deformability assays and microscopic imaging, it was demonstrated that phagocytic blood cells with internalized bacteria exhibit aberrant physical properties, which were leveraged to develop a novel microfluidics-based biosensor platform for phagocytosis detection. This platform showed promising clinical utility in rapidly diagnosing microbial infections, especially in bloodstream infections, ultimately improving point-of-care management outcomes.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Georgii Konoplev, Darina Agafonova, Liubov Bakhchova, Nikolay Mukhin, Marharyta Kurachkina, Marc-Peter Schmidt, Nikolay Verlov, Alexander Sidorov, Aleksandr Oseev, Oksana Stepanova, Andrey Kozyrev, Alexander Dmitriev, Soeren Hirsch
Summary: Proteins in biological fluids are important biomarkers for various pathological conditions. Portable diagnostic biosensor devices based on microfluidic technology are being used as an alternative to conventional laboratory protein assays. This review focuses on label-free techniques for protein detection and characterization in microfluidic structures, including optical and impedance spectroscopy techniques. The challenges of materials selection, surface tailoring, and sensitivity enhancement in biosensor systems are discussed.
Review
Nanoscience & Nanotechnology
Giovanni Nava, Giuliano Zanchetta, Fabio Giavazzi, Marco Buscaglia
Summary: Research in the field of optical biosensors is expanding due to the introduction of new technologies and innovative use of established methods. Label-free optical biosensors offer opportunities for rapid and compact diagnostic devices, as well as the detection of nanoscale objects with high sensitivity.
Review
Chemistry, Analytical
Yanting Yang, Ning Hu, Jinan Deng, Jun Yang
Summary: This paper reviews the recent advances in electrochemical sensing of vitamins and provides detailed descriptions of implementation strategies, performance, and the development of miniaturization devices. The challenges faced by electrochemical detection of vitamins are discussed and future developments are prospect.
Article
Chemistry, Analytical
Hanxiao Zhang, Albert Zehan Li, Juewen Liu
Summary: Using DNA staining dyes for label-free detection of aptamer binding has been done for a long time, but the cationic nature of the dyes can lead to unstable signals and false interpretations of results due to adsorption. This study evaluated the stability of the signal and found that a quartz cuvette provided a more stable signal compared to a polystyrene microplate, and equilibrating the system improved signal stability. The surfactant Triton X-100 was found to have the best protective effect against fluorescence signal decrease due to dye adsorption, and its use improved signal stability for different aptamers.
Article
Chemistry, Multidisciplinary
Tanchen Ren, Miribani Maitusong, Xuhao Zhou, Xiaoqian Hong, Si Cheng, Yin Lin, Junhui Xue, Dilin Xu, Jinyong Chen, Yi Qian, Yuwen Lu, Xianbao Liu, Yang Zhu, Jian'an Wang
Summary: Tissue engineering requires precise control of cell distribution in materials and structures. Current ink-based bioprinting methods face challenges in achieving universal applicability due to the specific conditions required for different inks. The Magneto-Archimedes effect based strategy offers a promising alternative by directly modulating cell locomotion without relying on inks. However, its widespread adoption has been limited by the complexity and precision of magnetic field distribution. By controlling the paramagnetic reagent concentration and magnet gaps, researchers have successfully patterned micrometer-scale cell assemblies with single or multiple cell types. This Mag-Arch patterning technique has potential applications in tissue engineering, regenerative medicine, and cell biology research.
Review
Materials Science, Multidisciplinary
Ozgecan Erdem, Esma Derin, Saeedreza Zeibi Shirejini, Kutay Sagdic, Eylul Gulsen Yilmaz, Selvin Yildiz, Garbis Atam Akceoglu, Fatih Inci
Summary: The healthcare system has shifted towards home-based and self-monitoring strategies, utilizing wearable technologies to track physiological parameters and monitor biochemical markers without disrupting daily routines. Carbon nanomaterials offer unique features for integration with smart materials and sensors, showcasing their potential in wearable technologies. Further integration with various devices and technologies is presented in this manuscript.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Todd A. Duncombe, Aaron Ponti, Florian P. Seebeck, Petra S. Dittrich
Summary: The UV-vis spectra-activated droplet sorter (UVADS) is a new tool for high-throughput label-free chemical identification and enzyme screening. It collects full UV-vis spectra, processes data quickly, and sorts droplets for analysis. This technology can distinguish different chemicals and measure enzyme activity.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Huan Wang, Lijun Cai, Dagan Zhang, Luoran Shang, Yuanjin Zhao
Summary: The research developed structural color micromotors for label-free multiplex assay, achieving high detection efficiency. These micromotors can realize multiplex label-free detection of ions based on their optical coding capacity and responsive behaviors, with improved detection sensitivity due to their autonomous motion.
Article
Multidisciplinary Sciences
Jesse Q. Zhang, Kai-Chun Chang, Leqian Liu, Zev J. Gartner, Adam R. Abate
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Article
Biochemical Research Methods
Kenneth H. Hu, John P. Eichorst, Chris S. McGinnis, David M. Patterson, Eric D. Chow, Kelly Kersten, Stephen C. Jameson, Zev J. Gartner, Arjun A. Rao, Matthew F. Krummel
Review
Cell Biology
Vasudha Srivastava, Tyler R. Huycke, Kiet T. Phong, Zev J. Gartner
CURRENT OPINION IN CELL BIOLOGY
(2020)
Article
Biology
Christopher S. McGinnis, David A. Siegel, Guorui Xie, George Hartoularos, Mars Stone, Chun J. Ye, Zev J. Gartner, Nadia R. Roan, Sulggi A. Lee
Summary: This study demonstrates that mixing PBMCs from unrelated donors under standard scRNA-seq sample preparation conditions does not induce an allogeneic response, and that certain PBMC preparation methods and sample multiplexing technologies can introduce technical artifacts into scRNA-seq data. These findings provide important benchmarks for future cross-sectional immunological scRNA-seq experiments.
Editorial Material
Chemistry, Physical
Zev J. Gartner, Jennifer L. Hu
Summary: This bioprinting approach utilizes organoid-forming stem cells as living ink within hydrogels, guiding tissue-scale self-organization to generate more realistic gastrointestinal and vascular tissue constructs.
Review
Biochemistry & Molecular Biology
Pierre-Francois Lenne, Edwin Munro, Idse Heemskerk, Aryeh Warmflash, Laura Bocanegra-Moreno, Kasumi Kishi, Anna Kicheva, Yuchen Long, Antoine Fruleux, Arezki Boudaoud, Timothy E. Saunders, Paolo Caldarelli, Arthur Michaut, Jerome Gros, Yonit Maroudas-Sacks, Kinneret Keren, Edouard Hannezo, Zev J. Gartner, Benjamin Stormo, Amy Gladfelter, Alan Rodrigues, Amy Shyer, Nicolas Minc, Jean-Leon Maitre, Stefano Di Talia, Bassma Khamaisi, David Sprinzak, Sham Tlili
Summary: The interactions between mechanics and biochemistry in cell and tissue organization have recently attracted renewed interest from various scientific disciplines, with advances in optical physics, microscopy, and computational image analysis enhancing our ability to observe spatiotemporal patterns. In addition, rapid progress in genetic, biophysical, and optogenetic manipulation tools, as well as theory and computing, have allowed for the construction of predictive models describing the emergence of cell and tissue dynamics from the coupling of biochemistry and mechanics.
Article
Developmental Biology
Lain Martyn, Zev J. Gartner
Summary: Embryonic tissue boundaries are crucial for cementing newly patterned structures during development and serving as organizing centers for subsequent rounds of morphogenesis. Understanding the complex functions of these boundaries is essential for creating better self-organizing tissues in basic science, drug development, and regenerative medicine.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Rogelio A. Hernandez-Lopez, Wei Yu, Katelyn A. Cabral, Olivia A. Creasey, Maria Del Pilar Lopez Pazmino, Yurie Tonai, Arsenia De Guzman, Anna Makela, Kalle Saksela, Zev J. Gartner, Wendell A. Lim
Summary: The study engineered a two-step positive-feedback circuit that enables human cytotoxic T cells to discriminate targets based on a sigmoidal antigen-density threshold. This circuit involves a low-affinity synthetic Notch receptor controlling the expression of a high-affinity CAR, leading to sharp discrimination between cancer cells and normal cells based on antigen density.
Article
Multidisciplinary Sciences
Jesse Q. Zhang, Christian A. Siltanen, Ata Dolatmoradi, Chen Sun, Kai-Chun Chang, Russell H. Cole, Zev J. Gartner, Adam R. Abate
Summary: An automated approach using a commercial array spotter was described for generating droplet libraries quickly and efficiently, making combinatorial applications more feasible in droplet microfluidics. The method can produce high diversity droplet libraries without the need for microfluidic expertise.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Katelyn A. Cabral, David M. Patterson, Olivia J. Scheideler, Russell Cole, Adam R. Abate, David Schaffer, Lydia L. Sohn, Zev J. Gartner
Summary: The DPAC micropatterning technique uses DNA hybridization to assemble cells on substrates, adhering to complementary DNA sequences, allowing for precise patterning of different cell types.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell Biology
Abigail A. Kindberg, Vasudha Srivastava, Jonathon M. Muncie, Valerie M. Weaver, Zev J. Gartner, Jeffrey O. Bush
Summary: The study reveals that EPH/EPHRIN signaling decreases the stability of heterotypic cell:cell contacts through increased cortical actomyosin contractility, driving cellular self-organization. The cell segregation driven by EPH/EPHRIN depends on actomyosin contractility but occurs independently of directed cell migration and without changes in cell adhesion.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Katelyn A. Cabral, Vasudha Srivastava, Austin J. Graham, Maxwell C. Coyle, Connor Stashko, Valerie Weaver, Zev J. Gartner
Summary: The construction of three-dimensional microvascular networks remains challenging. In this study, we used bioprinting to pattern endothelial cells and investigated the microenvironmental cues necessary for their self-organization into microvessels. We found that the inclusion of fibrillar matrices and the absence of certain proteins in the culture medium promoted the formation of stable and perfusable microvascular networks in as little as 3 days.
TISSUE ENGINEERING PART A
(2023)
Article
Biotechnology & Applied Microbiology
Jesse Q. Zhang, Christian A. Siltanen, Leqian Liu, Kai-Chun Chang, Zev J. Gartner, Adam R. Abate