Article
Chemistry, Multidisciplinary
Morgan D. McSweeney, Limei Shen, Alexander C. DeWalle, Jordan B. Joiner, Elizabeth C. Ciociola, Dharmendra Raghuwanshi, Matthew S. Macauley, Samuel K. Lai
Summary: This study explored the effectiveness of prophylaxis with free PEG or tolerogenic PEGylated liposomes as a strategy to reduce the amount of APA induced by subsequently administered PEGylated liposomes. The results showed that a single administration of free PEG alone was capable of markedly reducing the APA response to PEG-liposomes for up to 2 months, which was comparable to, and often exceeded, interventions with different tolerogenic PEG-liposomes. These findings support further research on free PEG prophylaxis as a potential strategy to mitigate the APA response to sensitizing PEGylated therapeutics.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Ahlem Meziadi, Navid Zuberi, Hendrick W. de Haan, Marc A. Gauthier
Summary: This study reveals that the inefficiency of bioconjugation reactions, such as protein PEGylation, is caused by the excluded volume effect (EVE) between methoxy poly(ethylene glycol) (mPEG) and proteins. The researchers found that this EVE leads to the self-assembly of proteins and reactive mPEG into separate protein-rich and mPEG-rich domains. By adding unreactive low-molecular weight mPEG as a co-solvent, the association between the larger protein and reactive mPEG can be promoted, resulting in improved reaction efficiency.
Article
Polymer Science
Mona Ebadi, Ahmad Rifqi Md Zain, Tengku Hasnan Tengku Abdul Aziz, Hossein Mohammadi, Clarence Augustine T. H. Tee, Muhammad Rahimi Yusop
Summary: Coated iron oxide nanoparticles were synthesized for the delivery of sorafenib and their effects on cancer cells were investigated. The study demonstrated that the use of polyethylene glycol-coated iron oxide nanoparticles could enhance the effect of sorafenib on cancer cells.
Article
Chemistry, Applied
A. S. Eremina, Yu Kargina, A. Yu Kharin, D. Petukhov, V. Yu Timoshenko
Summary: A one-step procedure using polyethylene glycol (PEG) coating during grinding in a planetary ball mill has been proposed to stabilize mesoporous silicon nanoparticles in aqueous solutions. The nanoparticles were found to have a size of 50-100 nm, preserved nanocrystallinity and mesopores, and remained stable in aqueous solution for at least 24 hours. This PEGylation method offers a way to control the physical properties and stability of mesoporous silicon nanoparticles for biomedical applications.
MICROPOROUS AND MESOPOROUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Asim Mushtaq, Li Li, A. Anitha, Lisbeth Grondahl
Summary: This study synthesized methoxy polyethyleneglycol grafted chitosan (mPEG-CHI) using carbodiimide-mediated coupling and evaluated the effect of reaction conditions on the degree of substitution and PEGylation yield. The optimized conditions resulted in a high DS and PEGylation yield, making the product suitable for medical applications. The cytotoxicity of mPEG-CHI was also assessed and found to be non-toxic against breast cancer cell lines.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Physical
Soheyla Karimi, Hassan Namazi
Summary: The study successfully fabricated a triazine dendrimer-functionalized graphene oxide nanocarrier for drug delivery systems with potential applications in cancer treatment. The results showed that the nanocarrier exhibited pH-responsive drug release behavior, excellent biocompatibility, and higher apoptotic effects on breast cancer cells.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Chemistry, Multidisciplinary
Guifeng Miao, Yuejian He, Keren Lai, Yan Zhao, Peiyi He, Guozhu Tan, Xiaorui Wang
Summary: This study reveals that commonly used PEG-based pharmaceutical excipients can induce the production of anti-PEG antibodies in vivo, which significantly impacts the pharmacokinetics and pharmacodynamics of PEGylated nanoparticles.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Analytical
Shixing Tang, Lin Li, Rui Wang, Sagar Regmi, Xinyu Zhang, Guoqiang Yang, Jian Ju
Summary: A novel colorimetric method for the determination of sialic acid (SA) was developed using 4-mercaptophenyl boronic acid (4-MPBA) as the analyte's recognition molecule, and negative charge PEG (400) was used to repulsive the interparticle crosslinking. The proposed sensing platform showed a linear relationship between the ratio of the absorbance intensity (A(525)/A(660)) and concentration of SA from 0.05 to 8 mM (R-2 = 0.997), with a detection limit of 48 mu M. This gold-based colorimetric sensor is easy to fabricate, reproducible in its test performance, and has been successfully applied for the detection of SA in biological and healthcare product samples.
Article
Chemistry, Multidisciplinary
Haruka Takata, Taro Shimizu, Rina Yamade, Nehal E. Elsadek, Sherif E. Emam, Hidenori Ando, Yu Ishima, Tatsuhiro Ishida
Summary: Modifying the surface of nanoparticles with PEG is commonly used to improve their stability and circulation half-lives. However, PEGylation may induce anti-PEG antibodies, affecting the efficacy of PEGylated therapies. This study investigated the production of anti-PEG antibodies in mice following different administration routes of PEGylated liposomes.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Taro Shimizu, Mizuki Awata, Amr S. Abu Lila, Chihiro Yoshioka, Yoshino Kawaguchi, Hidenori Ando, Yu Ishima, Tatsuhiro Ishida
Summary: In this study, we developed a delivery system using hydroxy PEG-modified liposomes (HO-PEG-Lip) that efficiently activated the complement system, facilitated the association with MZ-B cells, and significantly enhanced antigen delivery in vivo. The manipulation of HO-PEG density at 2 mol% in total lipids led to a successful induction of OVA-specific IgG response after a single intravenous injection. This new delivery technique may provide a simple and effective way to deliver antigens to MZ-B cells, inducing a significant humoral immune response.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Materials Science, Biomaterials
Kai Sugiura, Toshiki Sawada, Yuuki Hata, Hiroshi Tanaka, Takeshi Serizawa
Summary: This study demonstrates a simple method for distinguishing anti-PEG antibodies using antibiofouling cello-oligosaccharide assemblies tethering specific one-terminal methoxy oligo(ethylene glycol) ligands. The method allows for quantitative detection of the antibodies, even in the presence of serum.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Yixiang Cao, Shiyin Zhang, Ming Ma, Yu Zhang
Summary: In this study, we successfully prepared Fluorinated Cationic Polymer-Coated Magnetic Nanoparticles (FPP@MNPs) as carriers for siRNA. It was demonstrated that FPP@MNPs have good biocompatibility and high cellular uptake efficiency, and can successfully transfect cells and reduce the expression of CXCR4.
Article
Pharmacology & Pharmacy
Isaac H. Caballero-Floran, Hernan Cortes, Fabiola V. Borbolla-Jimenez, Carla D. Floran-Hernandez, Maria L. Del Prado-Audelo, Jonathan J. Magana, Benjamin Floran, Gerardo Leyva-Gomez
Summary: This study proposes the use of polyethylene glycol 400 (PEG) and trehalose as a surface modification technique to improve PLGA-based nanoparticles as drug carriers for neurons. The combination of PEG and trehalose enhances the hydrophilicity of the nanoparticles and promotes their cellular internalization. Through optimization using central composite design, PLGA nanoparticles with diameters smaller than 200 nm were produced, and the coating process did not significantly increase their size. The nanoparticles showed high curcumin entrapment efficiency and a controlled release profile.
Article
Chemistry, Physical
Mareike F. S. Deuker, Volker Mailaender, Svenja Morsbach, Katharina Landfester
Summary: Poly(ethylene glycol) (PEG) is commonly used to reduce unspecific protein adsorption and prolong nanocarrier circulation time, but the presence of anti-PEG antibodies in the bloodstream can counteract its stealth effect. This study found a high concentration and prevalence of anti-PEG antibodies in the German population, which led to higher uptake in macrophages and accelerated blood clearance of PEGylated nanocarriers.
NANOSCALE HORIZONS
(2023)
Article
Chemistry, Physical
Andrei Choukourov, Daniil Nikitin, Pavel Pleskunov, Renata Tafiichuk, Kateryna Biliak, Mariia Protsak, Ksenia Kishenina, Jan Hanus, Milan Dopita, Miroslav Cieslar, Tomas Popelar, Lukas Ondic, Marian Varga
Summary: Colloidal solutions of metal nanoparticles are usually produced using multi-step methods with various chemicals, but a single-step method proposed in this study only requires Cu NPs and polyethylene glycol, showing enhanced stability and optical performance.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Aaron Henry Bannister, Kyle Bromma, Wonmo Sung, Mesa Monica, Leah Cicon, Perry Howard, Robert L. Chow, Jan Schuemann, Devika Basnagge Chithrani
BRITISH JOURNAL OF RADIOLOGY
(2020)
Article
Engineering, Biomedical
Jan Schuemann, Alexander F. Bagley, Ross Berbeco, Kyle Bromma, Karl T. Butterworth, Hilary L. Byrne, B. Devika Chithrani, Sang Hyun Cho, Jason R. Cook, Vincent Favaudon, Yaser H. Gholami, Elisabetta Gargioni, James F. Hainfeld, Felicien Hespeels, Anne-Catherine Heuskin, Udoka M. Ibeh, Zdenka Kuncic, Sijumon Kunjachan, Sandrine Lacombe, Stephane Lucas, Francois Lux, Stephen McMahon, Dmitry Nevozhay, Wilfred Ngwa, J. Donald Payne, Sebastien Penninckx, Erika Porcel, Kevin M. Prise, Hans Rabus, Sharif M. Ridwan, Benedikt Rudek, Leon Sanche, Bijay Singh, Henry M. Smilowitz, Konstantin Sokolov, Srinivas Sridhar, Yaroslav Stanishevskiy, Wonmo Sung, Olivier Tillement, Needa Virani, Wassana Yantasee, Sunil Krishnan
PHYSICS IN MEDICINE AND BIOLOGY
(2020)
Article
Oncology
Kyle Bromma, Abdulaziz Alhussan, Monica Mesa Perez, Perry Howard, Wayne Beckham, Devika B. Chithrani
Summary: The use of gold nanoparticles (GNPs) as a treatment method can reduce side effects in cancer treatment and enhance targeted therapy. Testing nanomaterials in three-dimensional tumor models can better replicate the tumor environment, providing more accurate experimental data for future clinical research and treatment.
Review
Pharmacology & Pharmacy
Abdulaziz Alhussan, Ece Pinar Demirci Bozdogan, Devika B. Chithrani
Summary: About half of cancer patients receive radiotherapy for local tumors, but treatment doses are often limited by tissue toxicity. Radiosensitizing agents like gold nanoparticles and docetaxel can enhance damage to tumors while minimizing normal tissue toxicity. Combining these agents could potentially improve the effectiveness of radiation therapy and quality of life for cancer patients.
Article
Oncology
Abdulaziz Alhussan, Kyle Bromma, Monica Mesa Perez, Wayne Beckham, Abraham S. Alexander, Perry L. Howard, Devika B. Chithrani
Summary: Radiotherapy and chemotherapy are common methods of cancer treatment, but nanotechnology has emerged as a promising approach in cancer therapy. While most research has focused on tumor cells, targeting both tumor cells and cancer-associated fibroblasts with nanoparticles may enhance the therapeutic potential of cancer nanotechnology. Further studies are needed to explore the uptake and retention of nanoparticles in both tumor cells and CAFs for more effective treatment.
Article
Pharmacology & Pharmacy
Ocean Han, Kyle Bromma, Nicholas Palmerley, Ariadne T. Bido, Mesa Monica, Abdulaziz Alhussan, Perry L. Howard, Alexandre G. Brolo, Wayne Beckham, Abraham S. Alexander, Devika B. Chithrani
Summary: One of the major issues in current radiotherapy is the normal tissue toxicity. This study investigates the use of gold nanoparticles and bleomycin as a combination of radiosensitizers to enhance the effect of radiotherapy. The results show that the combination significantly reduces cell growth and is effective in causing DNA double-strand breaks. The incorporation of nanomedicine with radiotherapy has promising clinical applications.
Article
Oncology
Abdulaziz Alhussan, Nicholas Palmerley, Julian Smazynski, Joanna Karasinska, Daniel J. Renouf, David F. Schaeffer, Wayne Beckham, Abraham S. Alexander, Devika B. Chithrani
Summary: Many cancer therapeutics fail in clinical trials due to the complexity of the tumor microenvironment (TME). In this study, a simple tumor model consisting of two types of pancreatic cancer cells was used to test the efficacy of a co-culture vs. monoculture model. The co-culture model exhibited heightened resistance to radiation and the combination of gold radiosensitizers with radiotherapy reduced the radioresistance of the co-culture model. This study demonstrates the potential of using nanotherapeutics in targeting the complex TME.
Article
Oncology
Abdulaziz Alhussan, Nolan Jackson, Sarah Eaton, Nancy Dos Santos, Ingrid Barta, Josh Zaifman, Sam Chen, Yuen Yi C. Tam, Sunil Krishnan, Devika B. Chithrani
Summary: Pancreatic cancer is a major cause of cancer deaths worldwide. The use of nanoparticles as radiosensitizers and drug delivery vehicles can overcome current obstacles in cancer treatments. The study found that a DTX prodrug encapsulated in lipid nanoparticles can enhance the uptake of gold nanoparticles in pancreatic cancer, providing a potential strategy for radiotherapy.
Article
Pharmacology & Pharmacy
Nolan Jackson, Abdulaziz Alhussan, Kyle Bromma, David Jay, James C. C. Donnelly, Frederick G. G. West, Afsaneh Lavasanifar, Michael Weinfeld, Wayne Beckham, Devika B. B. Chithrani
Summary: This study aimed to explore the use of an antimalarial drug, PYD, as a DNA repair inhibitor to enhance the DNA damage induced by RT/GNP treatment. The results showed that PYD could significantly increase DNA double-strand breaks and inhibit cell proliferation in cancer cell lines. These promising findings could lay the foundation for the clinical application of this treatment modality.
Article
Oncology
Abdulaziz Alhussan, Kyle Bromma, Ece Pinar Demirci Bozdogan, Andrew Metcalfe, Joanna Karasinska, Wayne Beckham, Abraham S. Alexander, Daniel J. Renouf, David F. Schaeffer, Devika B. Chithrani
Summary: Pancreatic cancer is among the most fatal cancers, with a low five-year survival rate of 10%. This study focuses on the dynamics of gold nanoparticles within a pancreatic tumor microenvironment, revealing the uptake and retention of NPs by cancer cells and cancer-associated fibroblasts, potentially offering a paradigm-changing potential for combating the disease.