Journal
ACS SENSORS
Volume 3, Issue 5, Pages 953-959Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.7b00972
Keywords
immunoassay; CMOS; point-of-care; diagnostics; HIV; silver enhancement
Funding
- EPSRC [EP/K021966/1]
- Wellcome Centre for Molecular Parasitology [104111/Z/14/Z]
- EPSRC [EP/K021966/1] Funding Source: UKRI
- MRC [MC_PC_16045] Funding Source: UKRI
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Scalable immunoassay multiplexing offers a route to creating rapid point-of-care (POC) diagnostics. We present a method for multiplexing immunoassays on the surface of a complementary metal oxide semiconductor (CMOS) sensor array integrated circuit (IC) without the use of physical separators such as wells or channels. Major advantages of using a CMOS sensor array include low mass-manufacturing costs, the possibility to multiplex multiple assays on a single IC, and improved signal when averaging multiple sensors, along with providing a platform where wash steps can be incorporated to maximize selectivity and sensitivity compared to paper based lateral flow immunoassay. The device was able to differentiate between samples containing either, neither, or both rabbit anti-mouse (RAM) antibodies and/or anti-HIV gp120 antibodies in serum using a gold-nanoparticle promoted silver enhancement immunoassay. HIV antibody concentrations down to 100 mu g/mL were readily detected, which is three times lower than those typically found in infected humans (300-500 mu g/ mL), and the limit of detection was 10 mu g/mL.
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