Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Chemistry, Multidisciplinary
Jun Zhao, Jiandong Wu, Xiaosong Wu, Guoqing Deng, Yong Liu, Francis Lin, Ling Zhu
Summary: An efficient self-quenching fluorescence probe and the corresponding detection system were designed, capable of achieving a wide dynamic range and low detection limit in a single step. The method, with high specificity and biocompatibility, has great potential for accurately quantifying miRNA biomarkers in serum, providing promising prospects for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Shu-An Hsieh, Danial Shamsaei, Derek R. Eitzmann, Jared L. Anderson
Summary: This study demonstrates the use of a 3D printed droplet generation platform coupled with molecular beacons in LAMP detection, allowing for low-cost and customizable droplet generation. The study also demonstrates the use of a smartphone-based imaging system to increase accessibility. This method can be used for sequence-specific, sensitive, and accurate DNA quantification.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Hyojin Lee, Hyobeen Lee, Sang-Hyun Hwang, Woong Jeong, Dong-Eun Kim
Summary: This study developed a new method for sensitive detection of SARS-CoV-2 viral RNA without thermal cycling. By using tandem isothermal gene amplification technology, this method can detect very low concentrations of viral RNA within 1 hour, and it has high sensitivity and accuracy for COVID-19 diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Mai Zhang, Honghong Wang, Hui Wang, Fangfang Wang, Zhengping Li
Summary: A CRISPR/Cas12a-assisted sequence-specific detection method for LAMP products was developed to eliminate non-specific amplification effects. By designing target-specific stem-loop DNA probes, primer design for LAMP was greatly simplified, achieving sensitive detection of miRNAs with high specificity.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Huan Li, Yidan Tang, Defeng Song, Baiyang Lu, Lulu Guo, Bingling Li
Summary: Dual hairpin ligation-induced isothermal amplification (DHLA) is an effective nucleic acid amplification method, capable of accurately and flexibly detecting various targets with a detection limit down to the aM level without false-positive signals. This method allows direct detection of new targets by simply changing the probe sequence, without the need to redesign the primer set.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Jocelyn Reynolds, Reid S. Loeffler, Preston J. Leigh, Hannah A. Lopez, Jeong-Yeol Yoon
Summary: Isothermal nucleic acid amplification tests, which only require a constant temperature and simplify the amplification process, have gained popularity over PCR. Paper microfluidics have been extensively used for automated nucleic acid extraction, amplification, and detection in these isothermal amplification tests.
Article
Chemistry, Analytical
Guobin Han, Qiuyuan Lin, Jia Yi, Qian Lyu, Qingwei Ma, Liang Qiao
Summary: The researchers developed a new method for the rapid and accurate detection of SARS-CoV-2 using a three-way junction induced exponential rolling circle amplification (3WJ-eRCA) combined with MALDI-TOF MS assay. The method showed high specificity in distinguishing SARS-CoV-2 from other coronaviruses and demonstrated potential for large-scale screening of COVID-19.
Article
Chemistry, Analytical
Weiya Wang, Yuan Peng, Jin Wu, Man Zhang, Qiaofeng Li, Zunquan Zhao, Mingzhu Liu, Jiu Wang, Gaofang Cao, Jialei Bai, Zhixian Gao
Summary: A multi-step isothermal amplification fluorescence strategy was developed for quantitative detection of E2 at very low levels, with a good linear relationship between fluorescence value and E2 concentration. This method showed high sensitivity and specificity, providing a new possibility for highly sensitive detection of other small-molecule targets.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Na Wu, Hui-Chao Zhang, Xu-Hong Sun, Feng-Na Guo, Li-Xia Feng, Ting Yang, Jian-Hua Wang
Summary: By combining Exo III-assisted target recycling amplification with rolling circle amplification, a novel silver nanocluster was used as fluorescence probes to achieve ultrasensitive HIV/HCV DNA detection. Furthermore, through the design of padlock probes and utilization of generated ssDNA fragments, an intelligent DNA machine was constructed to distinguish multiple virus DNAs.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Analytical
Abudushalamu Gulinaizhaer, Mingyuan Zou, Shuo Ma, Yuming Yao, Xiaobo Fan, Guoqiu Wu
Summary: Nucleic acid testing is crucial for early diagnosis and monitoring of HIV and antiretroviral therapy outcomes. Isothermal nucleic acid amplification technology offers a solution for point-of-care molecular HIV detection in resource-limited areas, with high potential due to its improved efficiency and affordability.
Article
Chemistry, Analytical
Min Qing, Zhe Sun, Lei Wang, Shi Zhe Du, Jiao Zhou, Qian Tang, Hong Qun Luo, Nian Bing Li
Summary: The study combines electrochemical aptasensors with the CRISPR/Cas12a strategy, utilizing BIDSD, RCA, and CRISPR/Cas12a mechanisms to achieve specific and sensitive detection of thrombin with a calculated limit of detection of 1.26 fM, while simplifying the operation steps by omitting the probe surface-immobilization procedure.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Ya-ru Meng, Dandan Zhang, Xiaoran Zou, Fei Ma, Qi Kang, Chun-yang Zhang
Summary: Developed a label-free and sensitive method for NAD(+) assay using a trifunctional split dumbbell probe and ligation-triggered isothermal rolling circle amplification. The method exhibits high sensitivity with a detection limit of 85.6 fM, good selectivity, and potential applications in biological research and clinical diagnosis related to NAD(+).
Article
Physics, Condensed Matter
Sagar Ghorai, Sergey A. Ivanov, Ridha Skini, Peter Svedlindh
Summary: Polycrystalline La1-xPbxMnO3 +/- y solid solutions prepared by solid state reaction exhibit unique magnetic properties, with a second order paramagnetic to ferromagnetic phase transition and suppression of the Griffiths phase. The compounds show mean-field like behavior near their Curie temperatures, resulting from the formation of ferromagnetic clusters. The material with the lowest chemical substitution (x = 0.3) shows the highest potential as a magnetic refrigerant due to its higher relative cooling power and magnetic phase transition near room temperature.
JOURNAL OF PHYSICS-CONDENSED MATTER
(2021)
Article
Chemistry, Physical
Vitalii Shtender, Daniel Hedlund, Simon Rosenqvist Larsen, Peter Svedlindh, Martin Sahlberg
Summary: The Ce-Fe-Mn and Ce-Co-Mn systems have been re-investigated, leading to the discovery of two new potential phases for permanent magnets: Ce-3(Fe0.638Mn0.362)(29) and CeCo8Mn3. These compounds exhibit unique crystal structures and magnetic properties.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Materials Science, Multidisciplinary
Alena Vishina, Daniel Hedlund, Vitalii Shtender, Erna K. Delczeg-Czirjak, Simon R. Larsen, Olga Yu Vekilova, Shuo Huang, Levente Vitos, Peter Svedlindh, Martin Sahlberg, Olle Eriksson, Heike C. Herper
Summary: A new class of rare-earth-free permanent magnets based on Co3Mn2Ge has been proposed, demonstrating promising magnetic properties and potential applications. The discovery was a result of combining first principles theory with experimental synthesis and characterisation, showing the critical role of disorder in Co and Ge atoms for improving magnetism.
Article
Nanoscience & Nanotechnology
Shaquib Rahman Ansari, Nele-Johanna Hempel, Shno Asad, Peter Svedlindh, Christel A. S. Bergstrom, Korbinian Lobmann, Alexandra Teleki
Summary: This study introduces a novel application of magnetic hyperthermia to induce amorphization of a poorly aqueous soluble drug, celecoxib, in situ in tablets for oral administration. The degree of amorphization is strongly linked to the maximum tablet temperature achieved during the AMF exposure, which depends on the SPION composition and content in the tablets. The resulting maximum solubility of in situ amorphized celecoxib is 5 times higher than that of crystalline celecoxib in biorelevant intestinal fluid.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Anil Ahsan, Zhiwei Liu, Ruibing Su, Chencai Liu, Xiaoqi Liao, Min Su
Summary: Selenium has a significant chemo-preventive effect on esophageal squamous cell carcinoma by reducing cell proliferation, DNA damage, and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Zhongchao Huang, Jing Li, Hongwen Zhong, Bo Tian
Summary: This mini-review summarizes more than 30 publications on volume-amplified magnetic nanoparticle detection assays (VAMNDAs) and classifies their detection models for nucleic acid amplification (NAA)-induced MNP size increases. The performances of different linear, cascade, and exponential NAA strategies are highlighted. For some NAA strategies that have not yet been reported in VAMNDA, their performances are predicted based on reaction kinetics and feasible detection models. Challenges and perspectives are also discussed to inspire and guide future VAMNDA studies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Xiaozhou Xiao, Chuqi Yuan, Tingting Li, Jeppe Fock, Peter Svedlindh, Bo Tian
Summary: In comparison to alternative nanomaterials, magnetic micron/nano-sized particles offer unique advantages in terms of easy manipulation, stable signal, and high contrast. Optomagnetic biosensors, which utilize alternating magnetic fields to generate periodic movements of magnetic labels, provide a promising approach for point-of-care diagnostics. This review focuses on different concepts and strategies of optomagnetic biosensing techniques, including magnetic field-enhanced agglutination, rotating magnetic field-based particle rotation, and oscillating magnetic field-induced Brownian relaxation. Challenges and perspectives, such as minimizing nonspecific binding, on-chip integration, and multiplex detection, are also discussed.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Inorganic & Nuclear
Simon R. Larsen, Vitalii Shtender, Daniel Hedlund, Erna K. Delczeg-Czirjak, Premysl Beran, Johan Cedervall, Alena Vishina, Thomas C. Hansen, Heike C. Herper, Peter Svedlindh, Olle Eriksson, Martin Sahlberg
Summary: The atomic and magnetic structures of Mn(Co,Ge)2, which crystallizes in a superstructure of the MgZn2-type structure, have been reported. The system exhibits different magnetic structures at different temperatures.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jiaying Xiao, Chuqi Yuan, Mingming Ding, Wei Hu, Zhengwen Hu, Bo Tian
Summary: In this study, a homogeneous and isothermal photoacoustic biosensor is demonstrated for rapid amplification and detection of synthetic DNA related to SARS-CoV-2. The biosensor achieves a sub-femtomolar detection limit within a total assay time of 80 min and is highly specific for single-nucleotide polymorphism discrimination. It is also robust against background DNA interference.
Article
Biochemistry & Molecular Biology
Zhiwei Liu, Ruibing Su, Anil Ahsan, Chencai Liu, Xiaoqi Liao, Dongping Tian, Min Su
Summary: Squamous esophageal carcinoma is a common pathological type of esophageal carcinoma worldwide, with genomic instability observed during its development. This study suggests that DNA mutations caused by early DNA damage may serve as an indicator for ultra-early screening of esophageal cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Inorganic & Nuclear
Rebecca Clulow, Daniel Hedlund, Alena Vishina, Peter Svedlindh, Martin Sahlberg
Summary: A series of Fe5Si1-xGexB2 compounds were synthesized and their structural and magnetic properties were investigated. The compounds maintained the Mo5SiB2-type structure with Ge content below 0.15, and the unit cell parameters expanded as Ge content increased. The saturation magnetisation decreased slightly with increasing Ge content, while the magnetocrystalline anisotropy energy remained almost unchanged. The Curie temperature for all compounds studied was 790K, and the spin-reorientation temperature decreased from 172K to 101K at x = 0.15.
JOURNAL OF SOLID STATE CHEMISTRY
(2022)
Article
Chemistry, Analytical
Li Chen, Yuan Fang, Xuemei Zhou, Meng Zhang, Ruifeng Yao, Bo Tian
Summary: Plant microRNAs are important in post-transcriptional gene regulation, and there is a need for accurate and user-friendly microRNA detection methods to better understand plant growth, development, and stress responses.
ANALYTICAL CHEMISTRY
(2023)
Article
Gastroenterology & Hepatology
Chenxi Li, Zhaohui Liu, Guohua Xu, Shibin Wu, Yunhui Peng, Ruinuan Wu, Shukun Zhao, Xiaoqi Liao, Runhua Lin
Summary: Gastric cardiac intestinal metaplasia (IM) is more common in older age and males. The development of gastric cardiac IM is associated with promoter hypermethylation and downregulation of the EYA4 gene.
SAUDI JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Materials Science, Multidisciplinary
Konstantinos Papadopoulos, Ola Kenji Forslund, Elisabetta Nocerino, Fredrik O. L. Johansson, Gediminas Simutis, Nami Matsubara, Gerald Morris, Bassam Hitti, Donald Arseneau, Peter Svedlindh, Marisa Medarde, Daniel Andreica, Jean-Christophe Orain, Vladimir Pomjakushin, Lars Boerjesson, Jun Sugiyama, Martin Mansson, Yasmine Sassa
Summary: The magnetic phases of LaCaNiReO6 double perovskite were studied, revealing different magnetic regions and the evolution of interaction between Ni and Re magnetic sublattices.