4.2 Article

ID30B-a versatile beamline for macromolecular crystallography experiments at the ESRF

Journal

JOURNAL OF SYNCHROTRON RADIATION
Volume 25, Issue -, Pages 1249-1260

Publisher

INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600577518007166

Keywords

ID30B; MD2S; REX rapid nozzle changer; FlexHCD; SPINEplus gripper; experimental phasing; automation

Funding

  1. French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INBS-05]
  2. ESRF

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ID30B is an undulator-based high-intensity, energy-tuneable (6.0-20keV) and variable-focus (20-200 mu m in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was the last of the ESRF Structural Biology Group's beamlines to be constructed and commissioned as part of the ESRF's Phase I Upgrade Program and has been in user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating an in situ plate screening capability and a new flexible sample changer (the FlexHCD) were specifically developed for ID30B. Here, the authors provide the current beamline characteristics and detail how different types of MX experiments can be performed on ID30B (http://www.esrf.eu/id30b).

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