Article
Biochemistry & Molecular Biology
Joseph Saoud, Marie-Claude Carrier, Eric Masse, Sebastien P. Faucher
Summary: The study reveals that Lpr10 in Legionella pneumophila can negatively regulate the expression of the RpoS gene and is involved in a negative regulatory feedback loop to maintain optimal levels of RpoS expression.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Aurelien Croze, Antoine Carlino, Benjamin Quelard, Adeline Saha, Tiphaine Convert, Jean-Baptiste Eberst, Sandrine Demaneche
Summary: The study found that Legionella non-pneumophila (Lnp) strains interact differently with various amoebae when co-cultured at different temperatures. At lower temperatures, most Lnp strains, except for L. longbeachae, were phagocytosed by amoebae and unable to multiply intracellularly. At higher temperatures, some strains were able to hijack the machinery of amoebae for proliferation, but there were also specific amoebae that could prevent their growth.
Article
Biochemistry & Molecular Biology
Dominik A. Machtens, Jonas M. Willerding, Susanne Eschenburg, Thomas F. Reubold
Summary: The Gram-negative bacterium Legionella pneumophila is an accidental human pathogen that causes Legionellosis. During infection, L. pneumophila injects more than 300 effector proteins into the host cell to modify the intracellular environment and create a stable compartment for proliferation. The effector protein SidI inhibits host translation by interacting with eEF1A and may have a toxic effect due to its glycosyltransferase domain.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yongshan Gao, Xiaowen Yang, Lan Hua, Min Wang, Qing Ge, Weiqiang Wang, Na Wang, Jinming Ma, Honghua Ge
Summary: In this study, the crystal structure of Legionella pneumophila aspartate aminotransferase (Lpg0070) and its functionality were investigated. The results revealed the crucial role of the N-terminal arm in enzyme activity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Ivy Y. W. Chung, Lei Li, Oleg Tyurin, Alla Gagarinova, Raissa Wibawa, Pengfei Li, Elizabeth L. Hartland, Miroslaw Cygler
Summary: Legionella pneumophila is an intracellular pathogen that causes Legionnaire's disease in humans. It enters host cells through phagocytosis and affects host cellular processes by secreting over 300 effector proteins. RavA, a L. pneumophila effector, displays unique structural characteristics and localizes to the Golgi apparatus and plasma membrane in human cells.
Article
Microbiology
Mareike Kunze, Thomas Steiner, Fan Chen, Claudia Huber, Kerstin Rydzewski, Maren Staemmler, Klaus Heuner, Wolfgang Eisenreich
Summary: The metabolism of Legionella pneumophila strain Paris was studied during growth within its natural host Acanthamoeba castellanii. The results showed that bacterial infection increased the utilization of exogenous glucose by the host, while carbon fluxes via the host citrate cycle were not affected. The bacteria incorporated amino acids from the host into bacterial proteins during the infection cycle and showed partial de novo biosynthesis from exogenous serine and glucose.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Microbiology
Breanne M. Head, Christopher I. Graham, Teassa MacMartin, Yoav Keynan, Ann Karen C. Brassinga
Summary: The incidence of Legionnaires' disease, caused by Legionella pneumophila, is increasing. Little is known about the >25 different Legionella spp. that can cause disease in humans, but the use of a GFP-expressing plasmid may be a viable approach for real-time investigation of these non-pneumophila Legionella strains.
Article
Biology
Dario Huesler, Pia Stauffer, Bernhard Keller, Desiree Boeck, Thomas Steiner, Anne Ostrzinski, Simone Vormittag, Bianca Striednig, A. Leoni Swart, Francois Letourneur, Sandra Maass, Doerte Becher, Wolfgang Eisenreich, Martin Pilhofer, Hubert Hilbi
Summary: This study investigates the interaction between Legionella pneumophila and lipid droplets (LDs) in the amoeba Dictyostelium discoideum. The researchers find that the fusion GTPase Sey1, the T4SS of L. pneumophila, and the Ran GTPase activator LegG1 all promote the interaction between LDs and the LCV. In addition, Sey1 and the L. pneumophila fatty acid transporter FadL are implicated in fatty acid metabolism and intracellular growth of the bacteria.
Article
Biotechnology & Applied Microbiology
Lina Xiong, Mingchen Xia, Qinglin Wang, Zhen Meng, Jie Zhang, Guohui Yu, Zhangyong Dong, Yongjun Lu, Yunhao Sun
Summary: In this study, single-stranded DNA (ssDNA) aptamers against Legionella pneumophila were isolated and characterized using a whole-cell SELEX method. Four aptamers with strong affinity and selectivity for L. pneumophila were identified, and their binding to the bacteria was observed directly. These aptamers have the potential for use in medical diagnostics and public environmental detection assays for L. pneumophila.
BIOTECHNOLOGY LETTERS
(2022)
Review
Microbiology
Yongchao Xie, Yi Zhang, Yong Wang, Yue Feng
Summary: Legionella pneumophila is the causative agent of Legionnaires' disease, causing fever and lung infection. It secretes over 330 effectors to modulate host cellular processes, promoting bacterial growth. Among these effectors, SidE family proteins catalyze a non-canonical ubiquitination reaction and their activity is modulated by other effectors. Recent studies have provided insights into the modular structure of SidE family proteins and their role in pathogen virulence and further research.
Review
Cell Biology
Yi Yang, Ligang Mei, Jing Chen, Xiaorong Chen, Zhuolin Wang, Lu Liu, Aimin Yang
Summary: This article summarizes the posttranslational modifications of host proteins mediated by Legionella pneumophila effectors, including phosphorylation, ubiquitination, glycosylation, AMPylation, phosphocholination, methylation, ADP-ribosylation, as well as dephosphorylation, deubiquitination, deAMPylation, deADP-ribosylation, dephosphocholination, and delipidation. The molecular mechanisms and biological functions of these modifications in bacterial growth, Legionella-containing vacuole biosynthesis, and disruption of host immune and defense machinery are described.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Review
Microbiology
Ayesha Ayesha, Franklin Wang-Ngai Chow, Polly Hang-Mei Leung
Summary: Legionella pneumophila, an intracellular pathogen, utilizes OMVs to communicate with and modulate host immune response, playing a crucial role in disease pathogenesis. Understanding the functions of OMVs provides valuable insights for combating Legionnaires' disease.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Maura J. Donohue, Maily Pham, Stephanie Brown, Kaveri M. Easwaran, Stephen Vesper, Jatin H. Mistry
Summary: This study investigated the detection methods of Legionella pneumophila in tap water samples in buildings across the United States and their performance under different water quality conditions. The results showed variations in method performance when using three culture methods and one molecular method. Water quality influenced the detection results of L. pneumophila, especially the total organic carbon and heterotrophic bacterial counts were positively correlated with its detection frequency. Therefore, water quality should be considered when selecting L. pneumophila detection methods.
Review
Biochemistry & Molecular Biology
Yury Belyi, Nadya Levanova, Gunnar N. Schroeder
Summary: Work over the past two decades has revealed the significant role of glycosyltransferase effectors in the infection strategy of Legionella pneumophila, a Gram-negative respiratory pathogen. These effectors manipulate host protein synthesis, impacting the physiological state of target cells, such as cell cycle progression and immune and stress responses. Recent research has identified new glycosyltransferases and suggests the existence of undiscovered family members with unique catalytic features and substrates.
Article
Infectious Diseases
Jamie Gorzynski, Bryan Wee, Melissa Llano, Joana Alves, Ross Cameron, Jim McMenamin, Andrew Smith, Diane Lindsay, J. Ross Fitzgerald
Summary: Through whole-genome sequencing, this study investigates the evolutionary and epidemiological relationships of Legionella pneumophila isolates in Scotland over 36 years. The analysis reveals the presence of endemic clones in hospital, community, and travel-associated environments, and suggests the importance of redefining cluster and outbreak definitions. The study highlights the significance of whole-genome sequencing in real-time identification and mitigation of clinically important endemic clones.
Article
Biochemistry & Molecular Biology
Xiaojian Gong, Xiaolei Zhao, Wei Zhang, Jinzhao Wang, Xiaofang Chen, Muhammad Fazal Hameed, Nannan Zhang, Honghua Ge
Article
Biochemistry & Molecular Biology
Xiaofang Chen, Shan Liu, Sha Jiang, Xuecheng Zhang, Nannan Zhang, Jinming Ma, Honghua Ge
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Biochemical Research Methods
Xiaoyan Qu, Xiaowen Song, Nannan Zhang, Jinming Ma, Honghua Ge
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Yu Guo, Dan Zhou, Hui Zhang, Nan-Nan Zhang, Xiaoyu Qi, Xiaofang Chen, Qi Chen, Jing Li, Honghua Ge, Yan-Bin Teng
Summary: This study investigates the structural characteristics of MapA in HAPs and its substrate recognition mechanism. By analyzing the binding of the complex with inhibitors, a new substrate binding mode is revealed.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Plant Sciences
Zhongyan Yang, Nana Li, Takashige Kitano, Ping Li, Jennifer E. Spindel, Lishuo Wang, Genxiang Bai, Yiying Xiao, Susan R. McCouch, Atsushi Ishihara, Jili Zhang, Xin Yang, Zepeng Chen, Jianyu Wei, Honghua Ge, Georg Jander, Jian Yan
Summary: The accumulation of naringenin in rice is regulated by genes such as Os07g32020, which encodes a glycosyltransferase that promotes the conversion of naringenin. Modification of the Os07g32020 gene could be used to increase the production of naringenin and sakuranetin rice flavonoids in a more targeted manner. This opens up new opportunities for selective breeding of this important rice metabolic trait.
Article
Infectious Diseases
Muhammad Fazal Hameed, Yanan Chen, Ying Wang, Muhammad Shafiq, Hazrat Bilal, Linqing Liu, Jinming Ma, Pengying Gu, Honghua Ge
Summary: The study revealed the coexistence of mcr-1, bla(KPC-2), and bla(NDM-1) in clinical isolates from a tertiary hospital in Hefei, China. The prevalence of different Enterobacteriaceae sequence types, especially in E. coli, is concerning. Holding such resistance profiles poses a threat to humans and animals, requiring immediate and persistent control measures to address this hazard.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Chemistry, Multidisciplinary
Yan-Ni Zhang, Yuwei Zhang, Shan Su, Han-Ying Zhu, Wei Xu, Lu Wang, Meng Wu, Kai Chen, Fei-Qiang Yu, Tong-Kuai Xi, Qin Zhou, You-Hua Xie, Ximing Qin, Honghua Ge, Lu Lu, Jie Qing, Ge-Min Fang
Summary: The study introduces a dimeric ACE2 peptide mimetic with high binding affinity for SARS-CoV-2 spike RBD, capable of inhibiting the virus in cell cultures. It offers insights for optimizing peptide-based anti-SARS-CoV-2 inhibitors.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
LanDong Ma, XiaoLei Zhao, ZhiHang Zhang, HongHua Ge, Jin Wang
Summary: This study demonstrates a real time controllable assembling/aptasensing approach using plasmonic graphene oxide nanocomposites for tuning micro-nano structure and ultrasensitive detection of the MC-LR toxin. By combining DNA-hybridization and biotinstrepavidin binding protocol, the assembling process can be used for real time SERS aptasensing of MC-LR. This one-step nanofabrication and sensing strategy shows promising results for in-field testing of environmental contaminants.
Article
Microbiology
Di-Yin Li, Jian-Ting Han, Meng-Yuan Zhang, Xu Yan, Nannan Zhang, Honghua Ge, Zhiping Wang, Yong-Xing He
Summary: The two-component system RstA/RstB regulates the expression of multidrug resistance efflux pumps and enzymes involved in anaerobic nitrate respiration and pyoverdine biosynthesis, enhancing bacterial tolerance to antibiotics and hypoxic environments.
Article
Biochemistry & Molecular Biology
Nannan Zhang, Jin Wu, Siping Zhang, Maoran Yuan, Hang Xu, Jie Li, Pingping Zhang, Mingzhu Wang, Megan L. Kempher, Xuanyu Tao, Li-Qun Zhang, Honghua Ge, Yong-Xing He
Summary: The regulator PhlH from the rhizobacterium Pseudomonas fluorescens is able to coordinate the expression of enzymes involved in the biosynthesis of multiple secondary metabolites. Structural studies and molecular dynamics simulations reveal the ligand recognition and allosteric switching mechanisms of PhlH, and demonstrate that dissociation of 2,4-DAPG triggers a conformational transition by activating the DNA-binding domain of PhlH.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Gaojian Zhang, Jianxiong Han, Lili Wang, Xuegang Yang, Zhongkang Yan, Min Qu, Huijuan Zhou, Hazrat Bilal, Feifei Wang, Honghua Ge, Xingyuan Yang
Summary: This study reveals that STX11, a member of the SNARE family primarily expressed in immune cells, regulates lipid metabolism in hepatocytes by binding to ATGL and inhibiting lipid droplet degradation and lipid autophagy. This finding provides new insight into the regulation of hepatic lipid accumulation and related metabolic diseases.
Article
Cell Biology
Jiajia Gao, Wenwen Xu, Feng Tang, Minrui Xu, Qin Zhou, Xingyuan Yang, Nannan Zhang, Jinming Ma, Qi Yang, Xiaofang Chen, Ximing Qin, Honghua Ge
Summary: The intracellular pathogen Legionella pneumophila utilizes its type IVB secretion system (T4SS) to deliver the effector protein SidN into the nucleus of host cells. SidN disrupts the integrity of the nuclear envelope, leading to cell death and facilitating the pathogen's escape from the host for secondary infections.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiajia Gao, Wenwen Xu, Tianqi Liu, Wenjie Sun, Na Wang, Jinming Ma, Honghua Ge
Summary: In this study, the crystal structure of an N-acetyl sugar amidotransferase from Legionella pneumophila was determined, revealing its basin-shaped architecture and wide substrate-binding pocket. The structure suggests that the amidotransfer reaction catalyzed by this enzyme may require the conversion of ATP. These findings provide structural insights into the function of the NASAT protein family.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nanoscience & Nanotechnology
Xiaolei Zhao, Gangqiang Tao, Xiaojian Gong, Xingyuan Yang, Honghua Ge, Jin Wang
ACS APPLIED BIO MATERIALS
(2019)
