Article
Chemistry, Analytical
Feng Tian, Qi Wang, Chengfang Jiang, Mengmeng Li, Yuqi Zhang, Jing Yu, Fengyan Li, Tao Yan, Linlin Ren, Zhengxiao Gai, Xinyue Song, Shusheng Zhang
Summary: In this study, an endogenously and sequentially activated signal amplification strategy was constructed to achieve dual color fluorescence imaging of microRNA in living cancer cells. The designed nanoprobe could achieve high precision and reliability for microRNA bioimaging in cancer cells.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yuyan Li, Fanghua Zhang, Wendong Liu, Mingzheng Shao, Zhe Hao, Hongyan Zhang, Ruizhong Zhang, Xiyan Li, Libing Zhang
Summary: A DNA nanoprobe activated by glutathione (GSH) was developed for spatially selective sensing and imaging of miRNA in living cells. The nanoprobe consisted of metal-organic frameworks (MOFs) and DNA hairpin probes tethered to the MOF surface, with a disulfide bond in the hairpin loop. Cleavage of the disulfide bond by GSH triggered a strand-displacement reaction with target miRNAs, enabling fluorescence signal readout in situ. The combination of endogenous GSH activation and MOF improved the spatial resolution of miRNA detection and imaging.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Jiwei Wang, Caiyi Zhang, Zhao Liu, Shibao Li, Ping Ma, Fenglei Gao
Summary: A novel nanosystem has been developed for miRNA surface-enhanced Raman scattering (SERS)-fluorescence dual-mode dynamic monitoring and synergy phototherapy, improving the precision of tumor diagnosis and therapy.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Changchun Wen, Hua Chen, Xiaolu Guo, Zhaoxing Lin, Shuping Zhang, Xing-Can Shen, Hong Liang
Summary: By integrating CyHMC molecules onto AuNRs, a lysosome-targeted gold-based nanotheranostics AuNRs-CyHMC was successfully fabricated, combining the specificity of Raman spectrum, the speed of fluorescence imaging, and deep penetration of photo-acoustic imaging. The fluorescence and Raman signals in this system do not interfere, showing pH-sensitive Raman signals and lysosome-targeting ability.
Article
Chemistry, Multidisciplinary
Caiting Deng, Meichen Zheng, Shupeng Han, Yuanjie Wang, Jingqi Xin, Omer Aras, Liang Cheng, Feifei An
Summary: Sonodynamic therapy (SDT) is a promising cancer therapy that uses ultrasound to trigger a sonosensitizer to generate reactive oxygen species (ROS) with excellent tissue penetration depth. However, the spread of the sonosensitizer to healthy tissue beyond the tumor can cause undesired side effects. In this study, a GSH-activatable sonosensitizer prodrug is designed by attaching a quencher to tetraphydroxy porphyrin, which shows high tumor selectivity and activation in the presence of overexpressed GSH. The prodrug demonstrates effective tumor growth inhibition under ultrasound irradiation, highlighting its potential as a highly effective cancer treatment.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Xiaoxue Cheng, Xiaosong Li, Yuexi Kang, Decai Zhang, Qiubo Yu, Junman Chen, Xinyu Li, Li Du, Tiantian Yang, Yao Gong, Ming Yi, Songzhi Zhang, Shasha Zhu, Shijia Ding, Wei Cheng
Summary: This study proposes a new strand displacement reaction model to enhance the efficiency of RNA in situ imaging. The approach allows for monitoring changes in gene expression within cells and holds potential value for clinical applications.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xingya Jiang, Qinhan Zhou, Bujie Du, Siqing Li, Yingyu Huang, Zhikai Chi, William M. Lee, Mengxiao Yu, Jie Zheng
Summary: This study demonstrates a new method for non-invasively and consecutively monitoring hepatic glutathione depletion using in vivo fluorescence imaging and blood testing with the assistance of a body-clearable nanoprobe, potentially opening up new avenues for clinical disease detection.
Article
Biochemical Research Methods
Lixing Xu, Xing Du, Tianqing Liu, Dan Sun
Summary: Here, an excellent plasmonic nanoprobe was developed for real-time monitoring of intracellular GSH levels during ferroptosis, providing important theoretical support for elucidating the GSH-related metabolic mechanism of ferroptosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Lixing Xu, Xing Du, Tianqing Liu, Dan Sun
Summary: An excellent plasmonic nanoprobe was developed for real-time monitoring of intracellular GSH levels during ferroptosis. The nanoprobe, consisting of gold nanoparticles functionalized with crystal violet, detects GSH through the fluctuation in the SERS signal intensity. This low-cost, stable, and biocompatible nanoprobe enables dynamic monitoring of GSH levels in living cells during erastin-induced ferroptosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Ze Zhang, Yuyang Wu, Nan Lin, Shengyan Yin, Zihui Meng
Summary: MicroRNAs (miRNAs) play a significant role in genetic processes and human biological behavior, as well as in tumor pathology. Designing miRNA-sensitive fluorescent nanoprobes is a challenging research field. These nanoprobes can be used to determine pathological grading by comparing palette combinations based on specific miRNA binding within cancerous cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Rui Yang, Mengsi Zhan, Zhijun Ouyang, Honghua Guo, Jiao Qu, Jindong Xia, Mingwu Shen, Xiangyang Shi
Summary: The microfluidic synthesis of artesunate (ART)-loaded polydopamine (PDA)/iron (Fe) nanocomplexes (NCs) coated with fibronectin (FN) was reported in this study. These multifunctional Fe-PDA@ART/FN NCs exhibit desired properties for tumor therapy with improved chemodynamic therapy (CDT) and immunogenic cell death, and can be guided through magnetic resonance (MR) imaging. The developed FDRF NCs are considered an advanced nanomedicine formulation for chemo-chemodynamic-immune therapy of different tumor types under MR imaging guidance.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Analytical
Huo Xu, Min Lin, Yanhui Zheng, Xiaojun Fang, Xinmei Huang, Qi Huang, Jiawei Xu, Wei Duan, Juan Wei, Lee Jia
Summary: This study designed a multifunctional linear DNA nanostructure (MLN) as a carrier of molecular beacons (MB-21) for detecting and intracellular imaging miRNA-21. The MLN-MB exhibited a detection limit of 320 pM for miRNA-21 and high specificity against other miRNAs. With its outstanding stability, dual recognition, and biocompatibility, MLN-MB holds great potential for early cancer diagnosis.
Article
Chemistry, Multidisciplinary
Kok Ken Chan, Lin-Wei Shang, Zhen Qiao, Yikai Liao, Munho Kim, Yu-Cheng Chen
Summary: The study developed a peptide-encapsulated droplet microlaser to monitor the amyloidogenesis process and evaluate the effectiveness of anti-amyloid drugs. By extracting information from laser images, the progression of the amyloidogenesis process can be monitored using arrays of laser images from microdroplets. The results demonstrate the great potential of peptide microlasers for protein misfolding studies and high-throughput imaging of cavity biosensing.
Article
Chemistry, Analytical
Yu-Wen Zhang, Shan Li, Shu-Min Wang, Xiao-Qiong Li, Mei-Rong Cui, Bin Kang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We developed a miRNA-responsive DNAzyme cascaded catalytic (MDCC) nanomachine for amplified intracellular miRNA imaging and miRNA-guided efficient gene silencing. The intelligent MDCC nanomachine consists of multiple DNAzyme subunit-encoded catalyzed hairpin assembly (CHA) reactants sustained by pH-responsive Zeolitic imidazolate framework-8 (ZIF-8) nanoparticles. Through a catalytic hairpin assembly (CHA) reaction triggered by the presence of miRNA-21, a large number of Y-shaped fluorescent DNA constructs containing three DNAzyme modules are generated for gene silencing. This strategy provides a promising platform for highly sensitive determination of biomolecules and accurate gene therapy of cancer cells.
Article
Chemistry, Multidisciplinary
Guanglong Yuan, Jieqiong Cen, Jiamin Liao, Yuqin Huang, Liu Jie
Summary: The proposed strategy in this study involves loading aminoborane onto mesoporous polydopamine nanoparticles for hydrogen production, providing a novel approach for developing multifunctional nanosystems with high hydrogen-loading capacity and sustained release ability. The nanosystem not only achieves selective PTT/hydrogen therapies and US/CT bimodal imaging functions, but also shows excellent properties such as biocompatibility, promising as an efficient and safe tumor treatment for future clinical translation.