Journal
STEM CELL REPORTS
Volume 11, Issue 1, Pages 212-227Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2018.05.014
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Funding
- German Research Foundation - DFG [SFB-655]
- Fondazione Cenci Bolognetti
- Telethon [GGP15198]
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Bone, cartilage, and marrow adipocytes are generated by skeletal progenitors, but the relationships between lineages and mechanisms controlling their differentiation are poorly understood. We established mouse clonal skeletal progenitors with distinct differentiation properties and analyzed their transcriptome. Unipotent osteogenic and adipogenic cells expressed specific transcriptional programs, whereas bipotent clones combined expression of those genes and did not show a unique signature. We tested potential regulators of line-age commitment and found that in the presence of interferon-gamma (IFN gamma) adipogenic clones can be induced to osteogenesis and that their adipogenic capacity is inhibited. Analysis of IFN gamma-regulated genes showed that lineage signatures and fate commitment of skeletal progenitors were controlled by EGR1 and EGR2. Knockdown experiments revealed that EGR1 is a positive regulator of the adipogenic transcriptional program and differentiation capacity, whereas EGR2 inhibits the osteogenic program and potency. Therefore, our work revealed transcriptional signatures of osteogenic and adipogenic lineages and mechanism triggering cell fate.
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