Article
Multidisciplinary Sciences
Mario Gutierrez, Mark Shamoun, Katie Giger Seu, Tyler Tanski, Theodosia A. Kalfa, Omolola Eniola-Adefeso
Summary: In this study, a parameterization model of ektacytometry was used to quantify the bulk rigidity of rigid red blood cell populations in sickle cell disease patients. By coupling this model with an artificially rigid RBC model, an innovative system was formulated to estimate the average rigidity of rigid RBC populations in SCD blood, potentially offering valuable insights into disease severity assessment and treatment.
SCIENTIFIC REPORTS
(2021)
Article
Hematology
Okan Arihan, Elie Nader, Gokhan Oto, Yilmaz Kocak, Romain Fort, Philippe Connes
Summary: The study investigated the effects of chives plant aqueous extract on erythrocyte deformability of SCA patients. Results showed a significant decrease in erythrocyte deformability in SCA blood samples after exposure to the plant extract.
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
(2021)
Article
Biochemical Research Methods
Utku Goreke, Allison Bode, Sena Yaman, Umut A. Gurkan, Naside Gozde Durmus
Summary: This study developed a magnetic levitation-based assay for rapid and accurate analysis of density changes in red blood cells during disease states. By measuring the density of individual red blood cells, researchers identified different sub-populations of cells in blood samples from individuals with sickle cell disease, providing a method for further investigating blood cell disorders and the effects of therapies.
Article
Physiology
Alina Sadaf, Katie G. Seu, Elizabeth Thaman, Rose Fessler, Diamantis G. Konstantinidis, Holly A. Bonar, Jennifer Korpik, Russell E. Ware, Patrick T. McGann, Charles T. Quinn, Theodosia A. Kalfa
Summary: Oxyscan is an assay that measures the effects of deoxygenation and reoxygenation on red blood cell (RBC) deformability, gaining popularity in sickle cell anemia (SCA) research for its ability to predict complications and assess treatment response.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Medical Laboratory Technology
Melissa Azul, Eudorah F. Vital, Wilbur A. Lam, David K. Wood, Joan D. Beckman
Summary: This article provides an overview of the etiology and biological consequences of sickle cell disease. It also discusses the application and potential development of microfluidic technologies in sickle cell disease research.
TRANSLATIONAL RESEARCH
(2022)
Article
Physiology
Chih-Chin Hsu, Yu-Ting Lin, Tieh-Cheng Fu, Shu-Chun Huang, Cheng-Hsien Lin, Jong-Shyan Wang
Summary: Supervised cycling training (SCT) improves aerobic capacity and quality of life by enhancing erythrocyte deformability in patients with peripheral arterial disease (PAD).
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Physiology
M. C. Berrevoets, J. Bos, R. Huisjes, T. H. Merkx, B. A. van Oirschot, W. W. van Solinge, J. W. Verweij, M. Y. A. Lindeboom, E. J. van Beers, M. Bartels, R. van Wijk, M. A. E. Rab
Summary: Hereditary spherocytosis is a common form of hereditary chronic hemolytic anemia. Splenectomy, which is often performed in HS, leads to improvements in RBC properties such as intracellular viscosity and membrane stability. The Cell Membrane Stability Test (CMST) may serve as a novel biomarker for reflecting RBC membrane health and treatment response in HS.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Camille Boisson, Elie Nader, Celine Renoux, Alexandra Gauthier, Solene Poutrel, Yves Bertrand, Emeric Stauffer, Emilie Virot, Arnaud Hot, Romain Fort, Giovanna Cannas, Philippe Joly, Philippe Connes
Summary: This study found that both oxygen gradient ektacytometry and shear-stress-gradient ektacytometry are sensitive to disease activity in sickle cell disease (SCD). The deformability of red blood cells (RBCs) decreased during vaso-occlusive crisis (VOC) compared to steady state. The oxygen-dependent propensity of RBCs to sickle was highly variable during VOC.
Article
Physiology
Ankie M. van Cromvoirt, Simone Fenk, Ario Sadafi, Elizaveta V. Melnikova, Denis A. Lagutkin, Kuntal Dey, Irina Yu. Petrushanko, Inga Hegemann, Jeroen S. Goede, Anna Bogdanova
Summary: The deformability, lifespan, and rheological properties of red blood cells (RBCs) depend on factors such as cell deformability, hydration, and membrane stability. Age and gender may influence RBC parameters, with RBC age potentially affecting osmoscan parameters. Further studies are needed to validate age-stratified reference values for clinical practice.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Hematology
Nancy S. Green, Andrew Zapfel, Obiageli E. Nnodu, Patience Franklin, Venee N. Tubman, Lulu Chirande, Charles Kiyaga, Catherine Chunda-Liyoka, Bernard Awuonda, Kwaku Ohene-Frempong, Baba P. D. Inusa, Russell E. Ware, Isaac Odame, Emmanuela E. Ambrose, Livingstone G. Dogara, Assaf P. Oron, Chase Willett, Alexis A. Thompson, Nancy Berliner, Theresa L. Coetzer, Enrico M. Novelli
Summary: Sickle cell disease is a common condition in sub-Saharan Africa with high under-5 mortality. The American Society of Hematology established the Consortium on Newborn Screening in Africa (CONSA) to implement standardized newborn hemoglobinopathy screening and early intervention. The primary objectives of the trial are to determine the birth incidence of SCD and assess the effectiveness of early standardized care in reducing mortality. Secondary objectives include establishing universal screening and interventions and evaluating trial implementation.
Article
Zoology
Lev Nikolaevich Katiukhin, Shamil Ismailovich Chalabov, Kerim Sultanbekovich Bekshokov, Marya Sergeevna Pokhmelnova, Nisred Kadirovich Klichkhanov, Elena Romanovna Nikitina
Summary: The deformability and erythrocyte indices of ground squirrels vary seasonally, adapting to the need of oxygen supply to tissues. In spring, erythrocytes have the highest deformability, hydration, water permeability, and osmotic stability. In summer and autumn, there are changes in erythrocyte deformability, hydration, and osmotic stability, and an increase in hemoglobin concentration.
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2023)
Article
Hematology
Mukaddes Sinan, Ozlem Yalcin, Zeynep Karakas, Evrim Goksel, Nesrin Zeynep Ertan
Summary: This study found that zinc supplementation had positive effects on hemorheological parameters in patients with thalassemia major, increasing red cell deformability and reducing aggregation, thus improving microcirculation.
CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
(2023)
Article
Immunology
Margaux Chauvet, Cerina Chhuon, Joanna Lipecka, Sebastien Dechavanne, Celia Dechavanne, Murielle Lohezic, Margherita Ortalli, Damien Pineau, Jean-Antoine Ribeil, Sandra Manceau, Caroline Le Van Kim, Adrian J. F. Luty, Florence Migot-Nabias, Slim Azouzi, Ida Chiara Guerrera, Anais Merckx
Summary: This passage discusses the possible reasons for the high prevalence of sickle cell disease in certain populations may be related to the protective effect of HbS against severe malaria caused by Plasmodium falciparum. By studying protein phosphorylation, it reveals the impact of HbS heterozygous carriage on the phosphorylation of proteins in red blood cell membranes and skeletal proteins, as well as parasite proteins during infection with malaria, which may lead to a less severe manifestation of malaria symptoms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Medicine, Research & Experimental
Andrew M. Heitzer, Jennifer Longoria, Evadnie Rampersaud, Sara R. Rashkin, Jeremie H. Estepp, Victoria I. Okhomina, Winfred C. Wang, Darcy Raches, Brian Potter, Martin H. Steinberg, Allison A. King, Guolian Kang, Jane S. Hankins
Summary: The study aimed to evaluate the relationship between 11 SNPs in three HbF QTL and the intelligence of SCA patients. The results showed that HbF was positively associated with IQ, and HbF mediated the relationship between its QTL and IQ.
CURRENT RESEARCH IN TRANSLATIONAL MEDICINE
(2022)
Review
Oncology
A. M. Brandow, R. I. Liem
Summary: Sickle cell disease is a genetic disorder characterized by red blood cell deformation and end-organ damage. This review discusses advances in the diagnosis and management of major complications, as well as the latest developments in disease-modifying and curative therapies.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2022)
Letter
Hematology
Nermi L. Parrow, Pierre-Christian Violet, Nisha Ajit George, Faris Ali, Shivam Bhanvadia, Ryan Wong, John F. Tisdale, Courtney Fitzhugh, Mark Levine, Swee Lay Thein, Robert E. Fleming
Article
Medicine, General & Internal
Jonathan E. Sevransky, Richard E. Rothman, David N. Hager, Gordon R. Bernard, Samuel M. Brown, Timothy G. Buchman, Laurence W. Busse, Craig M. Coopersmith, Christine DeWilde, Wesley Ely, Lindsay M. Eyzaguirre, Alpha A. Fowler, David F. Gaieski, Michelle N. Gong, Alex Hall, Jeremiah S. Hinson, Michael H. Hooper, Gabor D. Kelen, Akram Khan, Mark A. Levine, Roger J. Lewis, Chris J. Lindsell, Jessica S. Marlin, Anna McGlothlin, Brooks L. Moore, Katherine L. Nugent, Samuel Nwosu, Carmen C. Polito, Todd W. Rice, Erin P. Ricketts, Caroline C. Rudolph, Fred Sanfilippo, Kert Viele, Greg S. Martin, David W. Wright
Summary: A trial on the effects of a combination treatment of vitamin C, thiamine, and hydrocortisone on ventilator- and vasopressor-free days in patients with sepsis did not show a significant increase within 30 days. However, the trial was terminated early and may have been underpowered to detect a clinically important difference.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2021)
Article
Pediatrics
Daniel Horim Choi, Sonali Basu, Mark Levine, David Steinhorn
Summary: This study aimed to describe the prevalence of ascorbic acid deficiency in critically ill pediatric patients. The study found that there is a wide variation in ascorbic acid levels among critically ill children with septic shock and/or respiratory failure requiring mechanical ventilation, and half of the patients had deficient levels of ascorbic acid.
JOURNAL OF PEDIATRIC INTENSIVE CARE
(2022)
Article
Nutrition & Dietetics
Ifechukwude Ebenuwa, Pierre-Christian Violet, Sebastian Padayatty, Yaohui Wang, Yu Wang, Henry Sun, Preston Adhikari, Sheila Smith, Hongbin Tu, Mahtab Niyyati, Kenneth Wilkins, Mark Levine
Summary: This study investigated the contribution, prevalence, and clinical characteristics of dysregulated vitamin C renal physiology in diabetes. The results showed a higher prevalence of vitamin C renal leak in participants with diabetes, which was associated with reduced plasma vitamin C concentrations. Glycemic control, microvascular complications, obesity, and proteinuria were predictive factors for renal leak.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Oncology
Agnes Basseville, Pierre-Christian Violet, Maryam Safari, Carole Sourbier, W. Marston Linehan, Robert W. Robey, Mark Levine, Dan L. Sackett, Susan E. Bates
Summary: This study identifies an association between the RAS-phenotype and sensitivity to histone deacetylase inhibitors in solid tumors. The researchers found that romidepsin treatment depletes acetyl-CoA, leading to metabolic and oxidative stress and ultimately causing death in KRAS-mutant cells. Analysis also revealed a correlation between acetyl-CoA generation pathways and HDACi sensitivity.
Article
Biology
Dagan Coppock, Pierre-Christian Violet, Gustavo Vasquez, Katherine Belden, Michael Foster, Bret Mullin, Devon Magee, Isabelle Mikell, Lokesh Shah, Victoria Powers, Brian Curcio, Constantine Daskalakis, Daniel Monti, Mark Levine
Summary: This randomized controlled trial investigated the impact of ascorbic acid on early clinical outcomes in COVID-19 patients. The results showed that ascorbic acid may be beneficial in reducing the use of supplemental oxygen, bronchodilators, and shortening the length of hospital stay in hospitalized patients. However, the beneficial effects of ascorbic acid diminished over the course of hospitalization. Future research should focus on the potential early benefits of ascorbic acid in hospitalized patients.
Article
Pharmacology & Pharmacy
Ping Chen, Greg Reed, Joyce Jiang, Yaohui Wang, Jean Sunega, Ruochen Dong, Yan Ma, Anna Esparham, Ryan Ferrell, Mark Levine, Jeanne Drisko, Qi Chen
Summary: This study investigated the pharmacokinetics of intravenous vitamin C (IVC) in healthy volunteers and cancer participants. The results showed that IVC was safe at appropriate doses, with complete renal clearance within 24 hours. These findings provide valuable information for future clinical trials.
CLINICAL PHARMACOKINETICS
(2022)
Article
Immunology
Ira Phadke, Marie Pouzolles, Alice Machado, Josquin Moraly, Pedro Gonzalez-Menendez, Valerie S. Zimmermann, Sandrina Kinet, Mark Levine, Pierre-Christian Violet, Naomi Taylor
Summary: The deficiency of vitamin C significantly impacts hematopoiesis, especially in the perinatal period, leading to a reduction in bone marrow cells and hematopoietic stem cells. Different subsets of myeloid progenitors have different sensitivity to vitamin C levels. Both perinatal and adult erythropoiesis are sensitive to vitamin C deprivation and can trigger splenic compensatory differentiation.
FRONTIERS IN IMMUNOLOGY
(2022)
Correction
Biology
Dagan Coppock, Pierre-Christian Violet, Gustavo Vasquez, Katherine Belden, Michael Foster, Bret Mullin, Devon Magee, Isabelle Mikell, Lokesh Shah, Victoria Powers, Brian Curcio, Daniel Monti, Mark Levine
Meeting Abstract
Oncology
Daniel Choi, Suvankar Majumdar, Mark Levine
PEDIATRIC BLOOD & CANCER
(2022)
Article
Nutrition & Dietetics
Maret G. Traber, Scott W. Leonard, Ifechukwude Ebenuwa, Pierre-Christian Violet, Mahtab Niyyati, Sebastian Padayatty, Sheila Smith, Gerd Bobe, Mark Levine
Summary: The study investigated the roles of the intestine and liver in alpha-tocopherol catabolism under conditions of fat or fasting, revealing differential catabolism of intravenous d(6)-alpha-tocopherol and oral d(3)-alpha-tocopherol. This suggests that both liver and intestine play a role in the metabolism of alpha-tocopherol, with variations in catabolite excretion observed between different interventions.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2021)