Review
Chemistry, Physical
Na Li, Qingqing Cai, Qing Miao, Zeshi Song, Yuan Fang, Bijie Hu
Summary: Sequencing technology is increasingly being used in clinical laboratories, with metagenomic next-generation sequencing (mNGS) emerging as an attractive method for comprehensive detection and classification of microorganisms. However, there are still several challenges to be overcome in clinical practice.
Article
Veterinary Sciences
Hongfei Shi, Bozhen Li, Jie Li, Shiwei Chen, Lulu Wang, Zhenzhen Bai, Li Zhu, Baolong Yan, Lunguang Yao
Summary: This study provides the first molecular evidence of natural Ca. M. haemobos infection in dogs and tick species H. longicornis and R. (B.) microplus from China.
BMC VETERINARY RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Godagama Gamaarachchige Dinesh Suminda, Srishti Bhandari, Yoonkyung Won, Umesh Goutam, Krishna Kanth Pulicherla, Young-Ok Son, Mrinmoy Ghosh
Summary: Globalization, agricultural intensification, urbanization, and climatic changes have led to an increase in emerging infectious zoonotic diseases. Innovative and effective research is needed to understand the transmission, ecological implications, and dynamics of these diseases at human-wildlife interfaces. High-throughput sequencing methodologies have the potential to improve detection and surveillance of zoonotic infections in livestock. This study investigates the current use of sequencing technologies and explores their potential for better infectious disease diagnosis and monitoring in farm animals.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Chuks Fidelis Nwanade, Zihao Wang, Ruwei Bai, Ruotong Wang, Tianai Zhang, Jingze Liu, Zhijun Yu
Summary: This study used whole-genome bisulfite sequencing to reveal the DNA methylation profile of Haemaphysalis longicornis under low-temperature stress for the first time. The study found that low temperature treatment led to changes in DNA methylation levels in the tick genome, with differentially methylated regions mainly enriched in binding and RNA transport pathways. These findings are important for understanding the response mechanisms of ticks to low-temperature stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Entomology
Ningmei Wang, Aimeng Ji, Abolfazl Masoudi, Shuang Li, Yuhong Hu, Yefei Zhang, Zhijun Yu, Han Wang, Hui Wang, Jingze Liu
Summary: In this study, the protein regulation mechanism of low-temperature tolerance in Haemaphysalis longicornis was investigated. The results showed that maintaining cell homeostasis, cell viability, and enhancing cell tolerance were crucial for the ticks to survive under low temperatures. Furthermore, arylamine N-acetyltransferase, inositol polyphosphate multikinase, and dual-specificity phosphatase were identified as potential key proteins in low-temperature tolerance.
Article
Agronomy
Matthew Bickerton, Julia Gonzalez, Andrea Egizi, Alvaro Toledo
Summary: The study evaluated the susceptibility of the Asian longhorned tick to various acaricides used in tick control. The results showed that the tick species is currently not resistant to the tested acaricides. Responsible integrated management and early detection of resistance are important for long-term efficacy of tick control products.
PEST MANAGEMENT SCIENCE
(2023)
Article
Infectious Diseases
Alec T. Thompson, Seth A. White, David Shaw, Kayla B. Garrett, Seth T. Wyckoff, Emily E. Doub, Mark G. Ruder, Michael J. Yabsley
Summary: The study on the phenology of Haemaphysalis longicornis in Virginia revealed seasonal differences in tick activity compared to previous studies in the northeastern United States. The presence of this exotic tick was found to be lower in field habitats and during the summer months, and it was detected on several wildlife hosts. Additionally, tick-borne pathogens were analyzed, including the detection of arthropod-borne pathogens native to the United States in host-seeking H. longicornis.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Entomology
Ki Beom Park, Jong Yul Roh, Seong Yoon Kim, Nam-Yeon Kim, Yong Hun Jo, Rae-Dong Jeong, Wook-Gyo Lee, Hee-Il Lee, Shin-Hyeong Cho, Yong Seok Lee, Yeon Soo Han
Summary: This study is the first to detect SFTSV from ticks using deep sequencing in Korea, revealing the genetic diversity of SFTSV in East Asia through analysis of different genomic segments and phylogenetic relationships.
ENTOMOLOGICAL RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Guanjie Lu, Zhe Wang, Baofeng Zhang, Zhichao Zhou, Defu Hu, Dong Zhang
Summary: This study used high-throughput 16S rRNA sequencing technology to identify the types and abundance of pathogenic bacteria in abscess disease samples. It found that the dominant pathogenic bacteria were Trueperella pyogenes, Fusobacterium necrophorum, and Bacteroides fragilis. The study also suggested that high-throughput sequencing technology has the potential to replace traditional bacterial culture and targeted PCR methods.
Article
Veterinary Sciences
Nneka Iduu, Subarna Barua, Shollie Falkenberg, Chance Armstrong, Jenna Workman Stockler, Annie Moye, Paul H. Walz, Chengming Wang
Summary: Theileria orientalis Ikeda genotype, a parasite causing economic challenges in cattle in Asia, New Zealand, and Australia, has been identified in Alabama, USA. The study found that infected cattle showed a high pathogen burden and the infection was persistent even in seemingly healthy cattle. Further investigation and surveillance of Theileria orientalis Ikeda and Haemaphysalis longicornis ticks in Alabama and other states, as well as evaluating the pathogenicity in cattle, are necessary.
VETERINARY SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Zhijun Yu, Biao He, Zhen Gong, Yan Liu, Qianghui Wang, Xiaomin Yan, Tiantian Zhang, Abolfazl Masoudi, Xu Zhang, Tianhong Wang, Xiaolong Yang, Hui Wang, Quan Liu, Jingze Liu, Changchun Tu
Summary: We report the sequencing, assembly, and annotation of the 3.16 gigabase genome of the tick Haemaphysalis longicornis. The genome of this species is larger than the previously assembled one and contains a high proportion of simple sequence repeats. Through genomic analysis, key genes involved in iron metabolism, detoxification, and freeze tolerance of H. longicornis were identified. Additionally, numerous endogenous viral elements were found within the genome. These findings provide insights into the genetic mechanisms of specific biological processes in ticks and lay the foundation for integrated control of ticks and tick-borne diseases.
Article
Agricultural Engineering
Chuks Fidelis Nwanade, Min Wang, Hongxia Li, Abolfazl Masoudi, Zhijun Yu, Jingze Liu
Summary: Plant essential oils can be used as alternatives to synthetic acaricides. The acaricidal activity of cinnamon essential oil is related to the presence of benzyl benzoate, which shows high toxicity against mites. Other compounds present in the oil can interact with benzyl benzoate to enhance its activity. Enzyme assays show that benzyl benzoate inhibits AChE activity. The ecotoxicological assessments indicate minimal effects on non-target terrestrial invertebrate, but toxicity to non-target aquatic species.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Ecology
Jacob Schultz, Phillip Scheuerman, Megan Quinn, Bill Brooks, Matthew Beer, Andrew Joyner
Summary: This study used species distribution modeling (Maxent) to predict suitable habitat areas for the invasive Asian longhorned tick in northeast Tennessee, and identified increasing human risk in the future. It suggests utilizing species distribution models as an effective tool for surveillance and control strategies against the spread of the Asian longhorned tick.
ECOLOGICAL INFORMATICS
(2023)
Article
Agronomy
Duo Wang, Ru Li, Ya-Xue Wu, Xiang-Yuan Fan, Xiao-Ya Liu, Feng Yang, Tian-Tian Zhang, Jing-Yi Ma, Yong-Hong Hu
Summary: This study reports the molecular characterization of the HK gene and its potential application in vaccines against Haemaphysalis longicornis. The results showed that protein and DNA-based vaccines could increase antibody levels in immunized animals. Vaccination trials on rabbits demonstrated that protein and DNA vaccines reduced tick infestation, egg mass weight, and egg hatching rate. Protein vaccination provided 65.6% protection against adult female ticks, while DNA vaccination provided 51.8% protection. In conclusion, the HK protein and DNA vaccines have potential as effective tick control tools and inhibiting glucose metabolism may be a new strategy for tick control.
PEST MANAGEMENT SCIENCE
(2023)
Article
Immunology
Maki Kuniyori, Nariko Sato, Naoaki Yokoyama, Shin-ichiro Kawazu, Xuenan Xuan, Hiroshi Suzuki, Kozo Fujisaki, Rika Umemiya-Shirafuji
Summary: In this study, the molecular mechanisms of transovarial transmission of the Babesia parasite in ticks were investigated. The expression profiles of key molecules involved in tick oogenesis were analyzed, revealing the impact of Babesia infection on yolk protein precursor synthesis and uptake. The accumulation of HIVg-2 was found to be associated with Babesia infection and transmission in ticks. These findings provide important insights into the transovarial transmission mechanisms of pathogens in tick vectors.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Virology
Xiankai Liu, Dongshu Wang, Chao Pan, Erling Feng, Hang Fan, Manli Li, Li Zhu, Yigang Tong, Hengliang Wang
ARCHIVES OF VIROLOGY
(2019)
Article
Microbiology
Can Wang, Puyuan Li, Wenkai Niu, Xin Yuan, Huiying Liu, Yong Huang, Xiaoping An, Hang Fan, Lilan Zhangxiang, Liyuan Mi, Jing Zheng, Yannan Liu, Yigang Tong, Zhiqiang Mi, Changqing Bai
RESEARCH IN MICROBIOLOGY
(2019)
Article
Virology
Hengyu Xi, Jiaxin Dai, Yigang Tong, Mengjun Cheng, Feiyang Zhao, Hang Fan, Xinwei Li, Ruopeng Cai, Yalu Ji, Changjiang Sun, Xin Feng, Liancheng Lei, Sadeeq ur Rahman, Wenyu Han, Jingmin Gu
Article
Virology
Mingming Gao, Yanhong Qin, Hang Fan, Xianglilan Zhang, Puyuan Li, Huiying Liu, Guangqian Pei, Zhiqiang Mi, Xuling Xu, Yigang Tong, Changqing Bai
ARCHIVES OF VIROLOGY
(2020)
Article
Virology
Tianyi Li, Shixing Tang, Yingying Su, Zuoyi Bao, Xiaolin Wang, Yongjian Liu, Hanping Li, Jingwan Han, Zhichao Pei, Zhengwei Wan, Hang Fan, Yigang Tong, Lin Li, Ning Wang, Jingyun Li
ARCHIVES OF VIROLOGY
(2020)
Article
Virology
Xin Qiang, Xueying Shen, Honghong Peng, Xiaofang Guo, Zhihai He, Mingguo Yao, Guozhang Fu, Yujun Cui, Xianglilan Zhang, Yong Huang, Hang Fan, Chunhong Du, Yigang Tong, Zhiqiang Mi
ARCHIVES OF VIROLOGY
(2020)
Letter
Public, Environmental & Occupational Health
Xiao-Ai Zhang, Hang Fan, Run-Zi Qi, Wei Zheng, Kui Zheng, Jian-Hang Gong, Li-Qun Fang, Wei Liu
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2020)
Article
Biochemistry & Molecular Biology
Na-Na Zhang, Xiao-Feng Li, Yong-Qiang Deng, Hui Zhao, Yi-Jiao Huang, Guan Yang, Wei-Jin Huang, Peng Gao, Chao Zhou, Rong-Rong Zhang, Yan Guo, Shi-Hui Sun, Hang Fan, Shu-Long Zu, Qi Chen, Qi He, Tian-Shu Cao, Xing-Yao Huang, Hong-Ying Qiu, Jian-Hui Nie, Yuhang Jiang, Hua-Yuan Yan, Qing Ye, Xia Zhong, Xia-Lin Xue, Zhen-Yu Zha, Dongsheng Zhou, Xiao Yang, You-Chun Wang, Bo Ying, Cheng-Feng Qin
Article
Infectious Diseases
Wenjuan Li, Xin Qiang, Si Qin, Yong Huang, Yan Hu, Bingke Bai, Jun Hou, Rong Gao, Xianglilan Zhang, Zhiqiang Mi, Hang Fan, Huahu Ye, Yigang Tong, Panyong Mao
INFECTION GENETICS AND EVOLUTION
(2020)
Article
Multidisciplinary Sciences
Hongjing Gu, Qi Chen, Guan Yang, Lei He, Hang Fan, Yong-Qiang Deng, Yanxiao Wang, Yue Teng, Zhongpeng Zhao, Yujun Cui, Yuchang Li, Xiao-Feng Li, Jiangfan Li, Na-Na Zhang, Xiaolan Yang, Shaolong Chen, Yan Guo, Guangyu Zhao, Xiliang Wang, De-Yan Luo, Hui Wang, Xiao Yang, Yan Li, Gencheng Han, Yuxian He, Xiaojun Zhou, Shusheng Geng, Xiaoli Sheng, Shibo Jiang, Shihui Sun, Cheng-Feng Qin, Yusen Zhou
Letter
Virology
Yawei Zhang, Xin Qiang, Xiaofang Guo, Honghong Peng, Si Qin, Yujun Cui, Hang Fan, Hongning Zhou, Jiusong Zhang, Jinglin Wang, Yigang Tong
Article
Biotechnology & Applied Microbiology
Rongrong Zhang, Feiyang Zhao, Jiuru Wang, Guangqian Pei, Hang Fan, Lilan Zhangxiang, Zhiqiang Mi, Taoxing Shi, Hui Liu, Yigang Tong
FOLIA MICROBIOLOGICA
(2020)
Article
Multidisciplinary Sciences
Zhiguang Xiang, Hua Zhu, Bochao Yang, Hang Fan, Jianguo Guo, Jiangning Liu, Qi Kong, Qingfeng Teng, Haiquan Shang, Lei Su, Chuan Qin
SCIENTIFIC REPORTS
(2020)
Article
Genetics & Heredity
Wanglin Liu, Mingyue Cheng, Jinman Li, Peng Zhang, Hang Fan, Qinghe Hu, Maozhen Han, Longxiang Su, Huaiwu He, Yigang Tong, Kang Ning, Yun Long
GENOMICS PROTEOMICS & BIOINFORMATICS
(2020)
Article
Infectious Diseases
Jin-Jin Chen, Xiao-Ai Zhang, Hang Fan, Fa-Chun Jiang, Mu-Zi Jin, Ke Dai, Ning Wang, Pan-He Zhang, Xiao-Kun Li, Hao Li, Wenqiang Shi, Zhi-Cong Yang, Li-Qun Fang, Hai-Sheng Zhou, Yue-Hong Wei, Wei Liu
INFECTION GENETICS AND EVOLUTION
(2020)