Journal
FRONTIERS IN PHARMACOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2018.00756
Keywords
Artemisia; Asteraceae; caffeoylquinic acids; flavonoids; high performance liquid chromatography; mass spectrometry; alpha-amylase inhibition; alpha-glucosidase inhibition
Categories
Funding
- Federal Agency of Scientific Organizations FASO [0337-2016-0006, AAAA-AA17-117011810037-0]
- Ministry of Education and Science of Russia [20.7216.2017/6.7]
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Artemisia genus of Asteraceae family is a source of medicinal plants known worldwide and used as ethnopharmacological remedies for the treatment of diabetes in Northern Asia (Siberia). The aim of this study was to determine the phenolic profile of 12 Siberian Artemisia species (A. anethifolia, A. commutata, A. desertorum, A. integrifolia, A. latifolia, A. leucophylla, A. macrocephala, A. messerschmidtiana, A. palustris, A. sericea, A. tanacetifolia, A. umbrosa) and to test the efficacy of plant extracts and pure compounds for antidiabetic potential. Finally, by HPLC-DAD-ESI-TQ-MS/MS technique, 112 individual phenolic compounds were detected in Artemisia extracts in a wide range of concentrations. Some species accumulated rare plant phenolics, such as coumarin-hemiterpene ethers (lacarol derivatives) from A. latifolia and A. tanacetifolia; melilotoside from A. tanacetifolia; dihydrochalcones (davidigenin analogs) from A. palustris; chrysoeriol glucosides from A. anethifolia, A. sericea, and A. umbrosa; eriodictyol glycosides from A. messerschmidtiana; and some uncommon flavones and flavonols. The predominant phenolic group from Artemisia species herb was caffeoylquinic acid (CQAs), and in all species, the major CQAs were 5-O-CQA (20.28-127.99 mu g/g) and 3,5-di-O-CQA (7.35-243.61 mu g/g). In a series of in vitro bioassays, all studied Artemisia extracts showed inhibitory activity against principal enzymes of carbohydrate metabolism, such as alpha-amylase (IC50 = 150.24-384.14 mu g/mL) and alpha-glucosidase (IC50 = 214.42-754.12 mu g/mL). Although many phenolic compounds can be inhibitors, experimental evidence suggests that the CQAs were key to the biological response of Artemisia extracts. Mono-, di- and tri-substituted CQAs were assayed and showed inhibition of alpha-amylase and a-glucosidase, with IC50 values of 40.57-172.47 mu M and 61.08-1240.35 mu M, respectively, and they were more effective than acarbose, a well-known enzyme inhibitor. The results obtained in this study reveal that Siberian Artemisia species and CQAs possess a pronounced inhibitory activity against alpha-amylase and alpha-glucosidase and could become a complement to synthetic antidiabetic drugs for controlling blood glucose level.
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