Article
Biophysics
Xiaotong Meng, Danny O'Hare, Sylvain Ladame
Summary: With the increasing demand for a new generation of nucleic acid tests, easily miniaturized electrochemical sensors show the greatest promise as they can be cost-effective, highly sensitive, and specific. These sensors rely on the immobilization of sequence-specific hybridization probes onto conductive materials or electrodes. This review covers various strategies for immobilizing nucleic acid probes onto different solid electrode materials, including both physical and chemical immobilization techniques.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Yongxin Zhai, Xiaoyu Zhu, Beining Xu, Ying Wang
Summary: An outstanding dual-labeling ratiometric electrochemical biosensor based on ISDPR has been proposed for highly sensitive and selective detection of mecA gene, which effectively integrates the advantages of signal amplification and ratiometric modes to achieve a broad working range and sensitive detection limit.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yachen Tian, Tao Liu, Cheng Liu, Qingqiang Xu, Qing Liu
Summary: CRISPR/Cas systems are developed as new biosensing tools for accurate and rapid pathogen detection. Researchers achieve signal amplification for detection by combining nanotechnology and various biosensor technologies, greatly improving the sensitivity and specificity of detection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Tong-Lin Hou, Liang Zhu, Xiao-Long Zhang, Ya-Qin Chai, Ruo Yuan
Summary: In this study, an intelligent multiregion linear DNA walker (MLDW) was developed for ultrasensitive detection of miRNA. By using the rolling-circle amplification mediated by target miRNA, the MLDW achieved a significantly improved reaction rate. The reaction could be completed within 30 minutes, which is at least 4 times faster than traditional DNA walkers. As a proof of concept, the high-efficiency MLDW was applied to an electrochemical biosensing platform and successfully detected target miRNA-21 with a detection limit of 36 aM. This innovative design of DNA walker provides new insights for constructing functional DNA nanodevices and promoting the research of nucleic acid signal amplification.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Tong Luo, Jiacheng Li, Yao He, Hui Liu, Zhiwei Deng, Xi Long, Qingqing Wan, Jiacheng Ding, Zan Gong, Yanjing Yang, Shian Zhong
Summary: In this study, we developed a novel CRISPR-based biosensor by combining CRISPR/Cas12a with Au-nanobeacon, which allows for direct and rapid detection of nucleic acids in complex biological fluids with high sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Huimin Hao, Ye Li, Bin Yang, Shuyan Lou, Zihua Guo, Weiyi Lu
Summary: In this study, a unique DNA probe with Hill-type cooperativity was developed based on toehold-mediated strand displacement processes, enabling accurate discrimination of single-nucleotide variants. The probe exhibited thermodynamic advantages for specificity, forming mismatch bubbles in the presence of single-nucleotide variants. The probe's feasibility was successfully demonstrated and its design allowed accurate discrimination of single-nucleotide variants, including insertions, mutations, and deletions, with Hill-type cooperativity serving as a novel strategy for DNA probe design.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Helena de Puig, Rose A. Lee, Devora Najjar, Xiao Tan, Luis R. Soekensen, Nicolaas M. Angenent-Mari, Nina M. Donghia, Nicole E. Weckman, Audrey Ory, Carlos F. Ng, Peter Q. Nguyen, Angelo S. Mao, Thomas C. Ferrante, Geoffrey Lansberry, Hani Sallum, James Niemi, James J. Collins
Summary: The miSHERLOCK is a low-cost CRISPR-based POC diagnostic platform that can rapidly detect SARS-CoV-2 and its variants with high sensitivity in just 1 hour. This modular system can be quickly adapted to different viruses and variants, and is complemented by a smartphone application for output interpretation and result reporting.
Article
Chemistry, Analytical
Qiuyuan Lin, Yuanwei Cao, Guobin Han, Wen Sun, Wenhao Weng, Hui Chen, Haoyi Wang, Jilie Kong
Summary: We report a general and programmable diagnostic platform that combines mesophilic Clostridium perfringens Argonaute (CpAgo) with exponential isothermal amplification (EXPAR) for the rapid and sensitive detection of multiple miRNAs with single-nucleotide discrimination in one pot. The CpAgo-based One-Pot (COP) assay achieved a limit of detection of 1 zMmiRNA within 30 min and showed superior analytical performance in distinguishing colorectal cancer patients from healthy individuals. This programmable, one-pot, multiplex, rapid, and specific strategy holds great promise in scientific research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Marjon Zamani, James M. Robson, Andy Fan, Michael S. Bono, Ariel L. Furst, Catherine M. Klapperich
Summary: Sexually transmitted infections, such as HIV and HPV, have a disproportionate impact on low-resource settings. Early diagnosis is crucial for managing HIV and preventing HPV-induced cervical cancer. A novel electrochemical platform combined with loop-mediated isothermal amplification and CRISPR-based detection shows promise for rapidly and cost-effectively detecting viral infections.
ACS CENTRAL SCIENCE
(2021)
Article
Chemistry, Analytical
Meiting Yang, Yidan Tang, Lijuan Qi, Sicai Zhang, Yichen Liu, Baiyang Lu, Jiaxue Yu, Kun Zhu, Bingling Li, Yan Du
Summary: A portable and accurate nucleic acid detection system was developed to address the urgent need for on-site virus screening and infection control. It utilizes ultrasensitive isothermal amplification to achieve rapid and sensitive detection of SARS-CoV-2 RNA, providing a cost-effective solution for emergency detection of the virus.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yi Zeng, Kevin M. Koo, Ai-Guo Shen, Ji-Ming Hu, Matt Trau
Summary: This study utilizes molecular hybridization chemistry to suppress noise signals and achieve ultraselective multiplexed detection of cancer gene fusion NA variants. By introducing modified LNA bases as locker probes and coupling them with customized SERS readout, detection of gene fusion NA variants in cancer cellular models was successfully realized.
Review
Chemistry, Analytical
Tibor Hianik
Summary: Early diagnostics of leukemia is critical for successful therapy, and biosensor technology plays an important role in improving detection methods of leukemia. DNA aptasensors have significant advantages over immunosensors in cancer cell detection.toUpperCase() acoustic technology is comparable in sensitivity to electrochemical methods, but is label-free and enables straightforward signal evaluation.
Article
Nanoscience & Nanotechnology
Marta Broto, Michael M. Kaminski, Christopher Adrianus, Nayoung Kim, Robert Greensmith, Schan Dissanayake-Perera, Alexander J. Schubert, Xiao Tan, Hyemin Kim, Anand S. Dighe, James J. Collins, Molly M. Stevens
Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.
NATURE NANOTECHNOLOGY
(2022)
Review
Chemistry, Inorganic & Nuclear
Zhi-Yuan Che, Xiao-Yan Wang, Xiaotian Ma, Shou-Nian Ding
Summary: The integration of bipolar electrode (BPE) with electrochemiluminescence (ECL), known as BP-ECL, shows great potential for analytical applications due to the excellent portability of BPE and intuitive readout of ECL. Signal amplification strategies are crucial for the development of highly sensitive BP-ECL sensing systems, and a systematic understanding of sensing formats and modes is meaningful for sensor design. An overview of signal amplification strategies and sensing formats in BP-ECL sensing assays holds promising potentials for analytical assays and can aid in understanding recent advances in the flourishing BP-ECL sensing fields.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemical Research Methods
Cristina Munoz-San Martin, Ana Montero-Calle, Maria Garranzo-Asensio, Maria Gamella, Victor Perez-Gines, Maria Pedrero, Jose M. Pingarron, Rodrigo Barderas, Noemi de-los-Santos-Alvarez, Maria Jesus Lobo-Castanon, Susana Campuzano
Summary: This work presents the first bioelectronic immunoplatforms for determining glycosylated haptoglobin (Hp) and both total and glycosylated Hp simultaneously. The immunoplatforms use non-competitive bioassays and magnetic microcarriers labeled with horseradish peroxidase (HRP) for detection. The method allows accurate determination of total and glycosylated Hp in colorectal cancer (CRC) cells, which is not possible with commercial ELISA kits and Western blot methodology due to lack of sensitivity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Esther Sanchez-Tirado, Lourdes Agui, Araceli Gonzalez-Cortes, Susana Campuzano, Paloma Yanez-Sedeno, Jose Manuel Pingarron
Summary: The study of the human microbiome is a multidisciplinary field that holds great potential for improving disease diagnosis and monitoring using microbiota biomarkers. Electrochemical biosensors, with their high sensitivity and fast response, combined with advancements in nanomaterials, offer unique tools for understanding the human microbiome and driving future developments. This review article focuses on the application of electrochemical (bio)sensors for monitoring microbial metabolites and disease biomarkers in the gastrointestinal microbiome, discussing challenges and future directions.
Article
Chemistry, Analytical
Beatriz Arevalo, Veronica Serafin, Maria Garranzo-Asensio, Ana Montero-Calle, Rodrigo Barderas, Paloma Yanez-Sedeno, Susana Campuzano, Jose M. Pingarron
Summary: This study presents the first amperometric biosensor capable of simultaneously determining the single or total content of various human immunoglobulin isotypes of anti-dsDNA antibodies, which are important biomarkers in autoimmune and neurodegenerative diseases. The biosensor involves the use of functionalized magnetic microparticles and specific secondary antibodies for detection. Clinical applications of the biosensor were successful in determining hIgs classes in healthy individuals, as well as patients with SLE and AD, yielding results consistent with established detection methods.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Victor Perez-Gines, Rebeca M. Torrente-Rodriguez, Maria Pedrero, Neus Martinez-Bosch, Pablo Garcia de Frutos, Pilar Navarro, Jose M. Pingarron, Susana Campuzano
Summary: Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related deaths in developed countries and predicted to be the second leading cause in 2030. A disposable amperometric immunoplatform for the determination of CA19-9 has been developed in this study. The immunoplatform combines magnetic microsupports (MBs) for immunoassay implementation and amperometric transduction on screen-printed carbon electrodes (SPCEs). The immunoplatform showed good reproducibility, selectivity, and storage stability, and was able to differentiate healthy subjects from PDAC patients within 1 hour. It represents an improvement in terms of cost, applicability, and accessibility compared to current ELISA-based techniques.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Susana Campuzano, Maria Gamella, Maria Pedrero, Jose M. Pingarron
Summary: The role of liquid biopsy in precision, equitable, and sustainable diagnosis of prevalent and highly heterogeneous diseases using biomarkers derived from whole cells, secretomes, and exosomes is undeniable. Electrochemical biosensing and biosensors, particularly those involving affinity reactions, have emerged as competitive and cost-effective analytical alternatives for identifying these challenging targets. This review provides a concise and up-to-date overview of the fundamentals, versatility, and opportunities offered by state-of-the-art electrochemical biosensing (2018-2022) for the determination of cellular-derived biomarkers. It also offers critical insights into the short-term challenges and future research directions in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Ana Montero-Calle, Maria Garranzo-Asensio, Rebeca M. Torrente-Rodriguez, Victor Ruiz-Valdepenas Montiel, Carmen Poves, Jana Dziakova, Rodrigo Sanz, Cristina Diaz del Arco, Jose Manuel Pingarron, Maria Jesus Fernandez-Acenero, Susana Campuzano, Rodrigo Barderas
Summary: The study aimed to assess whether p53 and p63 proteoforms derived from alternative splicing could have a differential seroreactivity and higher diagnostic value than canonical proteins in colorectal cancer. The researchers demonstrated the appearance of a differential seroreactivity among the proteoforms in colorectal cancer patients. These findings reveal increased complexity of the humoral immune response in cancer and highlight the potential for developing a blood-based biomarker signature for colorectal cancer detection.
Article
Chemistry, Analytical
Esther Sanchez-Tirado, Lourdes Agui, Marta Sanchez-Paniagua, Araceli Gonzalez-Cortes, Beatriz Lopez-Ruiz, Paloma Yanez-Sedeno, Jose M. Pingarron
Summary: In this study, an electrochemical immunoplatform was developed and applied for the simultaneous detection of four biomarkers for rheumatoid arthritis. Magnetic beads were used to immobilize specific antigens, and sandwich-type immunoassays were employed for the detection of the biomarkers. The results were validated against ELISA tests and certified values.
Review
Biochemical Research Methods
Susana Campuzano, Rodrigo Barderas, Maria Teresa Moreno-Casbas, Angeles Almeida, Jose M. Pingarron
Summary: In the era of personalization, personalized management of medicine and nutrition is crucial for life expectancy and quality of life. Implementing precision medicine and nutrition is a complex challenge that requires new technologies to meet cost, simplicity, and versatility requirements, as well as accurately determining molecular markers in biofluids at different omics levels almost in real time. This review critically discusses recent advances and positions electrochemical bioplatforms as suitable tools for advanced diagnostics, therapy, and precision nutrition.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Esther Sanchez-Tirado, Paloma Yanez-Sedeno, Jose Manuel Pingarron
Summary: Natural enzymes are used as special reagents in the preparation of electrochemical (bio)sensors, but their practical applications are limited due to certain drawbacks. Artificial enzymes based on nanomaterials, known as nanozymes, have been developed as an alternative, providing enhanced stability, low cost, easy production, and rapid activity. Among nanozymes, carbon-based nanozymes have been widely used in the preparation of electrochemical (bio)sensors due to their stable activity, tunability, and good biocompatibility.
Article
Chemistry, Multidisciplinary
Ponnusamy Nandhakumar, Cristina Munoz San Martin, Beatriz Arevalo, Shichao Ding, Mahika Lunker, Eva Vargas, Omeed Djassemi, Susana Campuzano, Joseph Wang
Summary: In order to address the low sensitivity issue of conventional LFA, we report a simple electrochemical LFA with nanocatalytic redox cycling for decentralized insulin detection. This method achieves higher sensitivity than traditional methods by amplifying the signal through nanoparticles and chemical reactions.
Review
Chemistry, Multidisciplinary
Susana Campuzano, Jose M. Pingarron
Summary: Electrochemical affinity biosensors are rapidly evolving and becoming increasingly important in our daily lives. They combine their intrinsic attributes with advances in technology to offer a wide range of opportunities and applications. This perspective provides an updated and critical view of these biosensors and discusses the challenges and future directions they face.
Article
Biochemistry & Molecular Biology
Sandra Tejerina-Miranda, Maria Pedrero, Marina Blazquez-Garcia, Veronica Serafin, Ana Montero-Calle, Maria Garranzo-Asensio, A. Julio Reviejo, Jose M. Pingarron, Rodrigo Barderas, Susana Campuzano
Summary: This study developed the first electrochemical bioplatform for determining human endostatin, which shows high sensitivity and selectivity for diagnosing tumors and identifying patients in different stages.
BIOELECTROCHEMISTRY
(2024)
