Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
Article
Environmental Sciences
M. Chiara Perego, Benjamin D. Mcmichael, Nicholas R. Mcmurry, Scott W. Ventrello, Lisa J. Bain
Summary: Exposure to arsenic during embryogenesis impairs neurodevelopment and locomotor activity. In this study, human-induced pluripotent stem cells were differentiated into motor neurons and exposed to environmentally relevant arsenic concentrations. The results showed that arsenic exposure dysregulated the differentiation of human induced pluripotent stem cells into motor neurons and impaired the cholinergic synapse pathway, suggesting impaired cholinergic function in motor neurons.
Article
Clinical Neurology
Jiali Pu, Lin Lin, Houbo Jiang, Zhixing Hu, Hong Li, Zhen Yan, Baorong Zhang, Jian Feng
Summary: In this study, researchers investigated the causal relationship between parkin mutations and dysfunction of human A9 dopaminergic neurons. They found that parkin mutations significantly reduced the frequency of pacemaking action potentials in these neurons. The results highlight the critical role of parkin in maintaining normal dopamine transmission.
MOVEMENT DISORDERS
(2023)
Article
Cell & Tissue Engineering
Salma Alasmar, Jez Huang, Karishma Chopra, Ewa Baumann, Amy Aylsworth, Melissa Hewitt, Jagdeep K. Sandhu, Joseph S. Tauskela, Robert N. Ben, Anna Jezierski
Summary: This study investigated the role of ice recrystallization inhibitors (IRIs) as cryoprotectants for iPSCs and iPSC-derived neurons (iPSC-Ns). The results showed that IRIs, specifically 2FA, increased the post-thaw viability and recovery of iPSCs without affecting pluripotency. Although 2FA supplementation did not significantly improve iPSC-N cell post-thaw viability, 2FA cryopreserved iPSC-Ns exhibited better restoration of neuronal network activity and synaptic function compared to controls. This study highlights the importance of optimizing cryopreservation media formulations with IRIs to improve functional cryopreservation of iPSCs and post-mitotic iPSC-Ns.
Article
Multidisciplinary Sciences
Kouya Uchino, Yasuyoshi Tanaka, Sayaka Kawaguchi, Kaori Kubota, Takuya Watanabe, Shutaro Katsurabayashi, Shinichi Hirose, Katsunori Iwasaki
Summary: Researchers developed an autaptic culture system containing human iPSC-derived astrocytes to study the effects of astrocytes on synaptic functions. They found that compared to neuron monocultures, the cultures with human iPSC-derived astrocytes exhibited significantly higher Na+ current amplitude, membrane capacitance, number of synapses, and dendrite length.
Article
Cell & Tissue Engineering
Somayeh Pouyanfard, Nairika Meshgin, Luisjesus S. Cruz, Karin Diggle, Hamidreza Hashemi, Timothy Pham, Manuel Fierro, Pablo Tamayo, Andrea Fanjul, Tatiana Kisseleva, Dan S. Kaufman
Summary: The study shows that human iPSC-derived macrophages can effectively reduce gene expression and pathological markers of liver fibrosis, offering a promising cell therapy approach to ameliorate fibrosis.
Article
Multidisciplinary Sciences
Devansh Agarwal, Rian Kuhns, Christos N. N. Dimitriou, Emmalyn Barlow, Karl J. J. Wahlin, Ray A. A. Enke
Summary: Researchers utilized human pluripotent stem cell-derived 3D retinal organoids and Next Generation Sequencing to explore the gene regulation and cell fate determination in human retinal development, providing valuable reference for vision research.
Article
Multidisciplinary Sciences
Izyan Mohd Idris, Fazlina Nordin, Nur Jannaim Muhamad, Julaina Abdul Jalil, Fatimah Diana, Aminn Ordin, Rosnani Mohamed, Adiratna Matripen, Gee Jun Tye, Wan Safwani Wankamarul Zaman, Muhammad Dain Yazid, Min Hweing
Summary: This study demonstrates that the fourth-generation lentiviral packaging system can produce high-titre lentiviruses for efficient generation of induced pluripotent stem cells (iPSCs). These iPSCs can maintain stability and differentiate into various cell types, including neural stem cells and neurons. The use of safer lentiviral packaging systems combined with established vector plasmids will help accelerate the clinical application of iPSCs.
Article
Cell Biology
Kenyi Saito-Diaz, Christina James, Archie Jayesh Patel, Nadja Zeltner
Summary: This study demonstrates the use of immunopanning method to isolate different subtypes of sensory neurons and successfully enriches the target subtypes with their respective subtype markers. It provides an effective method for further investigation of the development and diseases of sensory neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Tadahiro Shinozawa, Masaki Kimura, Yuqi Cai, Norikazu Saiki, Yosuke Yoneyama, Rie Ouchi, Hiroyuki Koike, Mari Maezawa, Ran-Ran Zhang, Andrew Dunn, Autumn Ferguson, Shodai Togo, Kyle Lewis, Wendy L. Thompson, Akihiro Asai, Takanori Takebe
Summary: This study established a screening model based on human liver organoids (HLO) for analyzing drug-induced liver injury (DILI) pathology, providing unique advantages at the organoid resolution. Through this model, a multi-readout organoid analysis was successfully developed to measure viability, cholestatic and/or mitochondrial toxicity, showing high predictive values for marketed drugs.
Review
Biochemistry & Molecular Biology
Mandeep Kumar, Nhung Thi Phuong Nguyen, Marco Milanese, Giambattista Bonanno
Summary: Astrocytes play a crucial role in neurodegenerative disorders, and the differentiation of human iPSCs into astrocytes has opened up new possibilities for disease modeling and drug screening. This advancement in research offers a promising avenue for exploring treatment strategies for neurodegenerative diseases.
Review
Cell & Tissue Engineering
Jiangtao Li, Xin Feng, Xiang Wei
Summary: This review summarizes the methods of establishing cardiomyocyte models using induced pluripotent stem cells (iPSCs) and the progress in genome editing techniques. Because the currently cultured iPSC-CMs are immature, researchers have attempted various methods to promote their maturation. Many researchers have established iPSC-CM models of hypertrophic cardiomyopathy (HCM) and used diverse methods for measurements.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Chih-Hsien Wu, Hsuan-Hwai Lin, Yi-Ying Wu, Yi-Lin Chiu, Li-Yen Huang, Cheng-Chung Cheng, Chung-Chi Yang, Tsung-Neng Tsai
Summary: Coronary artery disease is a major global issue. Patients with coronary artery disease often develop peptic ulcers when receiving aspirin treatment, making panendoscopy with biopsy necessary for diagnosis and treatment. A protocol for generating iPSCs from gastric fibroblasts via biopsy was established, with successful differentiation into functional cardiomyocytes demonstrated. This study represents the first in vitro generation of iPSCs from gastric fibroblasts.
Article
Multidisciplinary Sciences
Yin-Yu Lam, Chun-Ho Chan, Lin Geng, Nicodemus Wong, Wendy Keung, Yiu-Fai Cheung
Summary: Cardiomyocytes can be derived from hiPSC lines, but the efficacy varies. By performing single cell transcriptomics, researchers identified cardiac and extra-cardiac lineages during differentiation. They found APLNR as a surface marker for in vitro cardiac progenitors, and using this marker, they isolated and differentiated cardiac progenitors from multiple hiPSC lines. This study has potential applications in studying cardiac development, disease modeling, and cardiac regeneration.
Review
Biochemistry & Molecular Biology
S. A. Antonov, E. Novosadova
Summary: Human induced pluripotent stem cells offer new possibilities for Parkinson's disease research, allowing for investigation of disease pathogenesis, drug development, and personalized therapy. Despite the wide coverage of benefits in current literature, the drawbacks of the current model, particularly in differentiation protocols, are often overlooked. Further research is needed to improve differentiation conditions and increase efficiency for PD drug development using iPS cell-derived neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Bing Xie, David D. L. Minh
JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN
(2019)
Article
Engineering, Environmental
Dong Wu, Yinglong Su, Hui Xi, Xinyuan Chen, Bing Xie
Article
Chemistry, Multidisciplinary
Yongliang Zhang, Lu Zhan, Bing Xie, Zhenming Xu, Chao Chen
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2019)
Article
Engineering, Environmental
Xuemin Liu, Huahong Shi, Bing Xie, Dionysios D. Dionysiou, Yaping Zhao
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2019)
Article
Engineering, Environmental
Shengjia Ma, Jiana Jing, Pengyu Liu, Zongchen Li, Wei Jin, Bing Xie, Yaping Zhao
JOURNAL OF HAZARDOUS MATERIALS
(2020)
Article
Environmental Sciences
Shibo Lu, Rong Qiu, Jiani Hu, Xinyu Li, Yingxin Chen, Xiaoting Zhang, Chengjin Cao, Huahong Shi, Bing Xie, Wei-Min Wu, Defu He
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Environmental Sciences
Panliang Wang, Ziru Qiao, Xunan Li, Yinglong Su, Bing Xie
SCIENCE OF THE TOTAL ENVIRONMENT
(2020)
Article
Engineering, Environmental
Panliang Wang, Dong Wu, Xinxin You, Yinglong Su, Bing Xie
Summary: The presence of antibiotic and metal resistance genes in landfill environments is closely related to the concentrations of antibiotics and metals, as well as environmental factors such as nitrogen content. Dominant antibiotic and heavy metal resistance genes show significant correlation with environmental factors like nitrate and pH. The majority of sequenced resistance genes and nitrogen processing genes are hosted by specific bacterial species in leachates, indicating a complex interaction between genes and environmental conditions.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Xunan Li, Changbin Chu, Sheng Ding, Huawei Wei, Shuhang Wu, Bing Xie
Summary: This study investigated the effects of different fertilization strategies on soil properties, microbial community, and grape quality. The results showed that the combined application of organic and bioorganic fertilizers improved soil nutrients and promoted grape quality. However, excessive use of organic fertilizers derived from livestock manure could lead to heavy metal accumulation and affect grape quality.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Meilan Zhang, Kaiyi Li, Panliang Wang, Wenchao Gu, Huang Huang, Bing Xie
Summary: Mature landfill leachate is challenging to remove nitrogen and is an important source of antibiotic resistance genes (ARGs). Adding external carbon sources may promote the growth of bacteria carrying ARGs. This study investigated the effects of glucose, sodium acetate, and methanol supplements on ARGs. The results showed that sodium acetate reduced ARGs during the short-cut treatment process, while methanol increased ARGs during the whole-run treatment process. The study provides insights into ARGs during leachate treatment and suggests that sodium acetate is the optimal carbon source.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Engineering, Environmental
Liangmao Zhang, Binghan Wang, Yinglong Su, Dong Wu, Zijiang Wang, Kaiyi Li, Bing Xie
Summary: This study reveals the prevalence of antibiotic-resistant genes (ARGs) in PM2.5 from full-scale food waste treatment plants (FWTPs), highlighting the potential threat to human health. The ARGs were found to be significantly enriched in FWTP PM2.5, with Acinetobacter johnsonii-dominated pathogens playing a significant role in shaping and disseminating multidrug ARGs. The exposure level of resistant pathogens in FWTP PM2.5 was much higher than in urban PM2.5, emphasizing the need to reevaluate the air pollution caused by municipal FWTPs in terms of public health.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Qi Nie, Jingjing Deng, Bing Xie, Guoyue Shi, Tianshu Zhou
Summary: A dual-channel assay was developed using gold nanoparticles and copper nanoclusters for colorimetric and fluorescent detection of kanamycin. The assay showed a low limit of detection for kanamycin and offered great opportunities for on-site monitoring of antibiotics in environmental samples.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Jianhong Shi, Dong Wu, Yinglong Su, Bing Xie
ENVIRONMENTAL SCIENCE-NANO
(2020)
Article
Chemistry, Multidisciplinary
Jianhong Shi, Yinglong Su, Zhongjian Zhang, Huawei Wei, Bing Xie
ENVIRONMENTAL SCIENCE-NANO
(2019)
Article
Engineering, Chemical
Jianhong Shi, Yinglong Su, Chongxin Du, Bing Xie
DESALINATION AND WATER TREATMENT
(2019)
