Article
Engineering, Environmental
Ahmed Fergala, Ahmed AlSayed, Ahmed Eldyasti
Summary: The study explores the potential of coupling PHB consumption with methanol production using a PHB accumulating methanotrophic strain. Optimum cultivation conditions play a key role in increasing methanol production for methanotrophs. Cells depending on PHB solely showed improved methanol production when growth, PHB accumulation, and methanol production periods were adjusted. Additionally, PHB-rich cells supplied with 25 mM formate were able to produce a higher methanol concentration than PHB-lacking cells with 100 mM formate.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Rana Salem, Ahmed ElDyasti, Gerald F. Audette
Summary: Methanotrophic bacteria in wastewater treatment plants play a key role in producing valuable biomolecules for biotechnology and biomedicine, such as ectoine and polyhydroxyalkanoates. Their unique metabolism and biochemical pathways have potential biomedical applications in creating beneficial biocompatible products like vaccines and prosthetics.
Review
Environmental Sciences
Wei Wang, Yu Zhang, Tian -Ming Yin, Lei Zhao, Xi-Jun Xu, De-Feng Xing, Ruo-Chen Zhang, Duu-Jong Lee, Nan-Qi Ren, Chuan Chen
Summary: The introduction of denitrifying anaerobic methane oxidation (DAMO) technology provides a new method for wastewater treatment by utilizing methane as an electron donor instead of traditional organic carbon sources. DAMO can also achieve in-situ recycling of biogas, enhancing the environmental performance of wastewater treatment plants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
S. Gonzalez-Rojo, A. I. Paniagua-Garcia, R. Diez-Antolinez
Summary: This study explores the production and recovery of PHAs from potato processing wastewater. The optimal culture conditions for PHA synthesis by Cupriavidus necator DSM 545 were determined, resulting in a significant increase in PHB accumulation. In addition, a non-halogenated agent was found to be more effective in PHB recovery compared to the traditional chloroform method.
Article
Biotechnology & Applied Microbiology
Pranav Pradip Kulkarni, Vijaykumar Kashinath Khonde, Mandar Sachidanand Deshpande, Tushar Ramdas Sabale, Pramod Shankar Kumbhar, Anand Rameshchandra Ghosalkar
Summary: The study focused on selecting methanotrophs and investigating their methanol tolerance, production, and enzyme activities. M. alcaliphilum showed promising results for bio-methanol production, with high methanol tolerance and production levels. This systematic selection process identified M. alcaliphilum as a potential methanotroph for producing bio-methanol.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Polymer Science
Sanjay K. S. Patel, Rahul K. Gupta, In-Won Kim, Jung-Kul Lee
Summary: This study examines the influence of Cu and Fe nanoparticles on the growth of methanotrophs and the development of a polymeric-matrix-based encapsulation system for methanol production. The results show that the presence of nanoparticles and the use of simulated biogas feed greatly enhance methanol production, and the encapsulation system improves the stability and reusability of methanotrophs.
Article
Engineering, Environmental
Jorge Luis Meraz, Anthony J. Abel, Douglas S. Clark, Craig S. Criddle
Summary: Bioconversion of methane into PHB offers a promising solution for methane management. This study develops a dynamic model that accurately predicts the growth and PHB accumulation of Type II methanotrophs. The model explores the impact of various factors on productivity and energy efficiency and identifies operational windows for optimizing PHB production.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Pranav P. Kulkarni, Sambhaji B. Chavan, Mandar S. Deshpande, Dhanishta Sagotra, Pramod S. Kumbhar, Anand R. Ghosalkar
Summary: This study focuses on enriching methanotroph dominated mixed microbial community and investigating their ability to produce polyhydroxyalkanoates (PHA). The study found that Methylocystis was the dominant species and nitrate was the preferred nitrogen source for growth and PHA production. Under nitrate limited condition, the mixed culture accumulated a maximum of 22.20% polyhydroxybutyrate (PHB) with a molecular weight of 2.221 x 105 g mol-1 and a polydispersity of 1.82.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Liaofan Tang, Ji Li, Yong Li, Xiaolei Zhang, Xianbin Shi
Summary: Optimal denitrification was achieved at a ratio of 2:1 by adjusting the elemental sulfur content in the composite fillers, leading to an increase in microbial abundance. The biofilm composition transitioned from a single dominant species to multiple species, with an increase in genes related to sulfur metabolism.
Article
Environmental Sciences
Alessandro A. Carmona-Martinez, Eva Marcos-Rodrigo, Sergio Bordel, David Marin, Raquel Herrero-Lobo, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic digestion is a biotechnology for converting organic waste into biogas, but the decreasing renewable electricity prices require alternative uses for biogas. Recent efforts have focused on developing innovative platforms for producing chemicals from CH4, with ectoine being a prominent example. This study aimed to optimize the accumulation of ectoines using mixed microbial consortia from saline environments and activated sludge, with the enrichments showing different yields under varying NaCl concentrations and incubation temperatures.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Microbiology
Rob A. Schmitz, Nunzia Picone, Helena Singer, Andreas Dietl, Kerstin-Aniko Seifert, Arjan Pol, Mike S. M. Jetten, Thomas R. M. Barends, Lena J. Daumann, Huub J. M. Op den Camp
Summary: The methane-oxidizing bacterium Methylacidimicrobium thermophilum AP8 thrives in acidic geothermal ecosystems characterized by high methane degassing and lanthanide concentrations. Lanthanides, essential in high-tech devices, are actively taken up by methanotrophs/methylotrophs due to their XoxF-type methanol dehydrogenases requiring lanthanides as metal cofactors. Purification and characterization of a neodymium-containing methanol dehydrogenase from Methylacidimicrobium thermophilum AP8 provide insights into lanthanide biochemistry.
Article
Environmental Sciences
Ting Xie, Xinyu Liu, Yiming Xu, Samuel Bryson, Lu Zhao, Kai Huang, Shiqi Huang, Xiaoming Li, Qi Yang, Huiyu Dong, Mari-Karoliina H. Winkler
Summary: Simultaneous removal of ammonium and nitrate was achieved in a methane-fed moving bed biofilm reactor (MBBR) through the collaboration of methanotrophic microorganisms, denitrifiers, and anaerobic ammonium oxidizing bacteria.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Polymer Science
Rana Salem, Moomen Soliman, Ahmed Fergala, Gerald E. Audette, Ahmed ElDyasti
Summary: An efficient approach has been developed to maximize PHB polymer production using methane gas as a carbon substrate. A comparison study between new and conventional setups showed that the newly proposed setup successfully stimulated the growth of PHB accumulating type II methanotrophs and other PHB accumulators. The success of the method was confirmed by achieving highest recorded PHB accumulation percentages in both ammonia- and nitrate-enriched media.
Review
Microbiology
Shan Huang, Yuling Fu, Huimin Zhang, Chuqiao Wang, Chenglong Zou, Xiuguo Lu
Summary: This paper introduces the advantages and applications of novel bio-denitrification technologies, which can effectively reduce nitrogen levels in wastewater with lower energy consumption and operational costs compared to traditional methods.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Maria del Rosario Rodero, Alessandro A. Carmona-Martinez, Cristina Martinez-Fraile, Raquel Herrero-Lobo, Elisa Rodriguez, Pedro A. Garcia-Encina, Mar Pena, Raul Munoz
Summary: This study assessed the potential of producing ectoine from biogas under different reactor conditions and found that a stand-alone reactor demonstrated the best performance in ectoine accumulation and CH4 degradation.
