Article
Anatomy & Morphology
Caroline Gribner, Vanessa Barbosa, Luciane Mendes Monteiro, Juliane Nadal Dias Swiech, Vijayasankar Raman, Jane Manfron, Obdulio Gomes Miguel, Deise Prehs Montrucchio, Josiane de Fatima Gaspari Dias
Summary: This article investigates the morpho-anatomies of the leaves and stems of Ocotea paranaensis using microscopy techniques. The study reveals the presence of simple non-glandular trichomes, large secretory cells, unique petioles, and various cellular components such as starch grains and calcium oxalate crystals. The researchers also identified lignin, lipophilic contents, and phenolic compounds in different tissues. These findings have implications for the taxonomy and authentication of O. paranaensis.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Biology
Matheus Saukoski Pauzer, Thais de Oliveira Borsato, Valter Paes de Almeida, Vijayasankar Raman, Barbara Justus, Camila Bugnotto Pereira, Thiago Bevilacqua Flores, Beatriz Helena Lameiro Noronha Sales Maia, Emanuelle Kretz Meneghetti, Carla Cristine Kanunfre, Josiane de Fatima Padilha de Paula, Paulo Vitor Farago, Jane Manfron Budel
Summary: This study investigated the chemical composition and biological activities of Eucalyptus cinerea essential oil, finding it to possess antioxidant and antimicrobial properties with significant cytotoxicity against Calu-3 cells. Additionally, the anatomical features of the leaves and stems can aid in species identification and differentiation from other Eucalyptus species.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Edca Priscila Cardoso Ferreira Campos, Deysielle Maria dos Santos, Rafaela Damasceno Sa, Karina Perrelli Randau
Summary: The study aimed to anatomically and histochemically characterize the leaves of H. speciosa, revealing the presence of various compounds in the leaf blade, which contributes to quality control of the species and increasing knowledge about the Apocynaceae family.
MICROSCOPY AND MICROANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Claudia Giuliani, Martina Bottoni, Laura Santagostini, Alberto Spada, Alessio Papini, Fabrizia Milani, Gelsomina Fico
Summary: This study represents the first comprehensive investigation on Teucrium fruticans L. cultivated in Lombardy, Northern Italy. The study combined micromorphological and phytochemical surveys to analyze the plant's aerial parts. Micromorphological investigations revealed the presence of five distinct trichome morphotypes, while phytochemical characterization focused on the essential oils obtained from the plant. The essential oils were found to consist mainly of sesquiterpene hydrocarbons, with beta-caryophyllene and germacrene D identified as the main compounds.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Plant Sciences
Oluwatosin Temilade Adu, Yougasphree Naidoo, Temitope Samson Adu, Venkataramegowda Sivaram, Yaser Hassan Dewir, Hail Rihan
Summary: This study used light and electron microscopy techniques to examine the morphological and chemical features of secretory structures in Diospyros villosa, and identified medicinal compounds. These findings are important for establishing microscopic and pharmacognostic measures for future identification and verification of this herbal plant.
Article
Biochemistry & Molecular Biology
Luiz Renan Ramos da Silva, Zelina Ataide Correia, Ely Simone Cajueiro Gurgel, Olivia Ribeiro, Sebastiao Gomes Silva, Oberdan Oliveira Ferreira, Eloisa Helena de Aguiar Andrade, Mozaniel Santana de Oliveira
Summary: This study analyzed the anatomical characteristics and chemical composition of Plectranthus ornatus leaves. The results showed no significant differences between samples from different locations, and identified the major compounds in the essential oil.
Article
Plant Sciences
Irina Neta Gostin
Summary: The purpose of this study was to examine the morphological characteristics of glandular and non-glandular trichomes in leaves of Phlomis herba-venti subsp. Pungens using different microscopy techniques. Two types of non-glandular trichomes were identified: unicellular and branched, while glandular trichomes were categorized as capitate and dendroids. Histochemical analysis revealed distinct secretion products in each type of glandular trichomes. The dendroid trichomes were described and characterized for the first time in P. herba-venti.
Article
Biochemistry & Molecular Biology
Dipayan Ghosh, Neha Chaudhary, K. Uma Kumari, Jyoti Singh, Prakhar Tripathi, Abha Meena, Suaib Luqman, Anju Yadav, Chandan Singh Chanotiya, Gyanesh Pandey, Narendra Kumar
Summary: Magnolia sirindhorniae Noot. & Chalermglin produces fragrant flowers with volatile oil secretory cells that have higher density and size in flowers compared to buds, contributing to the synthesis of essential oil. The essential oil extracted from flowers exhibits stronger antimicrobial and antioxidant activities than that from buds.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Biochemistry & Molecular Biology
Flavio Polito, Florinda Fratianni, Filomena Nazzaro, Ismail Amri, Habiba Kouki, Marwa Khammassi, Lamia Hamrouni, Paola Malaspina, Laura Cornara, Sana Khedhri, Benedetta Romano, Daniela Claudia Maresca, Angela Ianaro, Giuseppe Ercolano, Vincenzo De Feo
Summary: Eucalyptus species are widely used in reforestation projects in Tunisia, despite the controversy surrounding their ecological functions. However, they play a significant role in combating soil erosion and serve as a fast-growing source of fuelwood and charcoal wood.
Article
Plant Sciences
Ana Margarida Rodrigues, Ana Rita Mendes, Maria Filomena Caeiro, Ana Cristina Figueiredo, Lia Ascensao
Summary: This study aimed to characterize the secretory structures of the endemic aromatic Asteraceae plant Santolina impressa, analyze its essential oil from the aerial organs, and evaluate its antiviral activity against herpes simplex viruses HSV-1 and HSV-2. The study found that Santolina impressa secreted an oleoresin and a resin rich in flavonoids through biseriate glandular trichomes and secretory ducts. The essential oil of Santolina impressa mainly consisted of monoterpenes, with β-pinene and β-phellandrene as the major components. The essential oil exhibited dose-dependent inhibition of HSV-1 and HSV-2 infections in Vero cells.
Article
Agricultural Engineering
Jie Zhang, Li Yu, Nuan Han, Dongmei Wang
Summary: This study analyzed the characteristics, accumulation kinetics, and tissue localization of phenolic compounds in Zanthoxylum bungeanum leaves. Four chemotypes were identified and the main components accumulated mainly in the epidermis, palisade tissue, and parenchyma cells.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biology
Larissa Vitoria dos Santos, Camila Dias Machado, Felipe de Oliveira Mendonca, Aline da Silva Justo, Vijayasankar Raman, Jane Manfron
Summary: This study characterizes Acmella bellidioides using microscopy and microchemical techniques to provide pharmacobotanical data for the authentication of the species. The notable anatomical markers identified include hypostomatic leaves, anomocytic stomata, peltate glandular trichomes, midrib vascular system, and prismatic crystals.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2023)
Article
Environmental Sciences
Chunyu Jiang, Shixing Zhou, Lin Liu, Zokir Toshmatov, Ling Huang, Kai Shi, Chi Zhang, Hua Shao
Summary: The chemical profile and phytotoxicity of Artemisia absinthium essential oil were investigated, identifying linalool as the major active compound responsible for its suppressive activity on receiver species. This study highlights the potential value of the essential oil and its major constituents as environmentally friendly herbicides.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemical Research Methods
Kexin Hu, Qimeng Sun, Ruifen Chen, Tinghao Xu, Yuncheng Li, Lili Chen, Aidong Wang, Hejing Qi, Danni Shao, Huanning Yue, Yaning Wang, Ziqi Tang, Yi Wang, Chunfeng Liu, Haijun Lv, Fen Wang, Huizhong Xu
Summary: Recently, a new fluorescent covalent staining method has been developed for visualizing anatomical structures in cells and tissues. This method utilizes carboxylate and phosphate stains, which provide higher signal intensity compared to existing stains. It allows more accurate identification of nucleoli in cancer cells and reveals a variety of sub-cellular structures when combined with existing amine stains in expansion microscopy samples. Furthermore, this staining method enables imaging of lipid-based structures in cultured cells, expanding the toolset for histological fluorescence visualization.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemistry & Molecular Biology
Wojciech Smulek, Maciej Jarzebski, Marek Ochowiak, Magdalena Matuszak, Jan Kaczorek, Jerzy Stangierski, Jaroslaw Pawlicz, Pawel Drobnik, Piotr T. Nowakowski, Joanna Dyrda-Muskus, Grzegorz Fiutak, Mieczyslaw Gorzelak, Sirsendu S. Ray, Kunal Pal
Summary: Nowadays, the search for new ways to fight pathogens, which are becoming more resistant to drugs, antibiotics, and antiviral medicaments, is intensively studied. Natural products, particularly essential oils, are being investigated as alternatives to synthesized compositions due to their known antimicrobial properties. In this study, the droplet size distribution and other properties of a particular essential oil composition called five thieves' oil (5TO) were evaluated. The study found that 5TO solutions or emulsion systems could potentially be used for active antimicrobial applications, such as surface spraying.
Article
Biochemistry & Molecular Biology
Abbas Ali, Nurhayat Tabanca, Betul Demirci, Vijayasankar Raman, Jane M. Budel, K. Huesnue Can Baser, Ikhlas A. Khan
Article
Anatomy & Morphology
Vijayasankar Raman, Jane Manfron, Ikhlas Ahmed Khan
Summary: This study provides a detailed description of the morpho-anatomical and histochemical characteristics of the leaves, peduncles, stems, and roots of Eryngium yuccifolium Michx., including unique features such as leaf structure, stomatal characteristics, and mesophyll structure. These characteristics can be used for the taxonomy, species identification, and quality control of this plant species.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Plant Sciences
Anelise Samara Nazari Formagio, Wagner Vilegas, Carla Roberta Ferreira Volobuff, Candida Aparecida Leite Kassuya, Valter Paes de Almeida, Jane Manfron, Zefa Valdevina Pereira, Marcia Regina Pereira Cabral, Maria Helena Sarragiotto
Summary: This study investigated the potential antioxidant, anti-acetylcholinesterase, antihyperalgesic, anti-nociceptive and anti-inflammatory properties of the methanolic extract of Palicourea tomentosa leaves. The results showed significant inhibition of pain and inflammatory parameters by the extract, supporting its traditional analgesic use.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Anatomy & Morphology
Caroline Gribner, Vanessa Barbosa, Luciane Mendes Monteiro, Juliane Nadal Dias Swiech, Vijayasankar Raman, Jane Manfron, Obdulio Gomes Miguel, Deise Prehs Montrucchio, Josiane de Fatima Gaspari Dias
Summary: This article investigates the morpho-anatomies of the leaves and stems of Ocotea paranaensis using microscopy techniques. The study reveals the presence of simple non-glandular trichomes, large secretory cells, unique petioles, and various cellular components such as starch grains and calcium oxalate crystals. The researchers also identified lignin, lipophilic contents, and phenolic compounds in different tissues. These findings have implications for the taxonomy and authentication of O. paranaensis.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Materials Science, Multidisciplinary
Vijayasankar Raman, Jane Manfron, Bharathi Avula, Jianping Zhao, Kumar Katragunta, Amar G. Chittiboyina, Ikhlas A. Khan
Summary: This paper provides a comparative analysis of the morpho-anatomical features of the roots and rhizomes of five species of Glycyrrhiza. The studied species showed similarities in basic anatomical features but differences in microscopic features.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Agronomy
Carolina C. Saulle, Alexandre Claus, Leticia de A. Sales, Alan G. Goncalves, Diogo R. B. Ducatti, Miguel D. Noseda, Louise L. May De Mio
Summary: Photodynamic inactivation (PDI) can be an important alternative method to combat major grain legume pathogens, significantly reducing infection rates through the combined action of photosensitizers, light, and molecular oxygen.
Article
Plant Sciences
Janaine Alberto Marangoni, Jannaina Velasques da Costa Pinto, Candida Aparecida Leite Kassuya, Pedro Cruz de Oliveira Jr, Sidney Mariano dos Santos, Claudia Andrea Lima Cardoso, Rosilda Mara Mussury Franco Silva, Marcia Espindola da Silva, Camila Dias Machado, Jane Manfron, Anelise Samara Nazari Formagio
Summary: This study investigated the chemical composition of the essential oil of Terphosaphis terebinthifolia collected in six states of Brazil and demonstrated its anti-inflammatory and antihyperalgesic effects. The micromorphology and histochemistry of leaves and stems provided useful information for species identification.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Anatomy & Morphology
Paola Aparecida Raeski, Gustavo Heiden, Andressa Novatski, Vijayasankar Raman, Ikhlas Ahmed Khan, Jane Manfron
Summary: This study provides a detailed analysis of the different types of calcium oxalate crystals in the genus Baccharis and highlights their significance for taxonomical classification. The study examines the morphology, occurrence, and chemical composition of crystals found in the stems and leaves of 44 species within the genus. The crystal macropatterns observed aid species identification and contribute valuable data for the taxonomy of Baccharis.
MICROSCOPY RESEARCH AND TECHNIQUE
(2023)
Article
Chemistry, Medicinal
Vijayasankar Raman, Yan-Hong Wang, Seethapathy G. G. Saroja, Jianping Zhao, Jane Manfron, Bharathi Avula, Amar G. G. Chittiboyina, Ikhlas A. A. Khan
Summary: This study provides an orthogonal approach to differentiate the dream herb from non-bitter calea using three independent methodologies. Detailed morpho-anatomical and histochemical characterizations of the two species were established, along with the identification of chemical compounds present. The study results would aid in authentication of botanical raw materials and quality control of Calea ternifolia-based products.
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2023)
Article
Biology
Vanessa Barbosa, Valter Paes de Almeida, Jane Manfron, Vijayasankar Raman, Camila Freitas de Oliveira, Fernando Cesar Martins Betim, Luiza Stolz Cruz, Juliane Nadal Dias Swiech, Vinicius Berdnarczuk de Oliveira, Josiane de Fatima Padilha de Paula, Obdulio Gomes Miguel
Summary: This study evaluates the in vitro antioxidant potential of B. erioclada crude extract and its fractions, as well as quantifies the total phenolics and flavonoids. The crude extract showed higher antioxidant capacity and lower IC50 values or greater reductive capacity compared to the fractions. The total phenolic compounds and flavonoids in the crude extract were quantified as 160.66 mg/g and 47.98 mg/g, respectively. Hispidulin content in the dried extract was 27,2296 g/100 g. B. erioclada extracts are promising sources of antioxidant compounds.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2023)
Article
Biology
Kevin Alves Antunes, Luciane Mendes Monteiro, Valter Paes de Almeida, Luan Baldiserra Junior, Vijayasankar Raman, Jane Manfron
Summary: Ilex paraguariensis, commonly known as yerba mate, is a shrub or tree native to South America with various medicinal properties. However, it is often adulterated with other species or unrelated plants. This study provides a comparative anatomy of the leaves and stems of I. paraguariensis and Citronella gongonha, which can aid in the authentication and quality control of yerba mate.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2023)
Article
Biology
Larissa Vitoria dos Santos, Camila Dias Machado, Felipe de Oliveira Mendonca, Aline da Silva Justo, Vijayasankar Raman, Jane Manfron
Summary: This study characterizes Acmella bellidioides using microscopy and microchemical techniques to provide pharmacobotanical data for the authentication of the species. The notable anatomical markers identified include hypostomatic leaves, anomocytic stomata, peltate glandular trichomes, midrib vascular system, and prismatic crystals.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2023)
Article
Forestry
Izabel P. Migacz, Jane Manfron, Paulo Farago, Vijayasankar Raman, Graciela I. B. de Muniz, Silvana Nisgoski
Summary: This study aimed to verify differences in VIS/NIR spectra and leaf color parameters of different ages of Eucalyptus leaves and their influence on species discrimination. Results showed that age had varying influences on the different species, with chromatic coordinate b* showing the most variation in color data. The study highlights the potential utility of color parameters and VIS/NIR spectroscopy for discriminating Eucalyptus species based on their green leaves.
Article
Pharmacology & Pharmacy
Vanessa Barbosa Bobek, Luiza Stolz Cruz, Camila Freitas de Oliveira, Fernando Cesar Martins Betim, Juliane Nadal Dias Swiech, Daniela Gaspardo Folquitto, Carmen Antonia Sanches Ito, Jane Manfron Budel, Sandra Maria Warumby Zanin, Josiane de Fatima Padilha de Paula, Obdulio Gomes Miguel
Summary: This study characterized the essential oil of Baccharis erioclada and evaluated its antioxidant, antimicrobial, and hemolytic potential. The essential oil exhibited antioxidant activity and showed moderate antimicrobial activity and low hemolysis rates.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Chemistry, Medicinal
Wagner D'Almeida, Luciane Mendes Monteiro, Vijayasankar Raman, Junaid Ur Rehman, Katia Sabrina Paludo, Beatriz Helena Lameiro de Noronha Sales Maia, Inaiara Casapula, Ikhlas Ahmed Khan, Paulo Vitor Farago, Jane Manfron Budel
Summary: This study investigated the chemical composition, cytotoxicity, and insecticidal activity of the volatile oil from Eugenia involucrata DC., Myrtaceae, and also provided detailed anatomical features of the leaves and stems. The main volatile compounds identified were germacrene B, bicyclogermacrene, and 13-elemene. Cytotoxicity was demonstrated in certain cell lines, and the volatile oil induced characteristic morphological changes in the cell death process.
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
(2021)