Article
Plant Sciences
Xiao Yin, Qingqing Fu, Boxing Shang, Yunlei Wang, Ruiqi Liu, Tingting Chen, Gaoqing Xiang, Mengru Dou, Guotian Liu, Yan Xu
Summary: This study reports an RxLR effector, RxLR50253, from Plasmopara viticola that interferes with plant immune response, promoting pathogen colonization in tobacco and grapevine leaves. The host target of RxLR50253 was identified as VpBPA1, whose interaction with RxLR50253 was confirmed through multiple assays. Further analysis revealed that VpBPA1 promotes pathogen colonization and decreases H2O2 accumulation. This study uncovers the mechanism by which Plasmopara viticola attenuates plant immunity during pathogen infection.
Article
Plant Sciences
Tao Ma, Shuyun Chen, Jiaqi Liu, Peining Fu, Wei Wu, Shiren Song, Yu Gao, Wenxiu Ye, Jiang Lu
Summary: Plasmopara viticola, the causal organism of grapevine downy mildew, secretes a variety of effectors to manipulate host immunity. A study found that the effector PvRXLR111 interacts with the plant transcription factor VvWRKY40 to suppress host immunity, suggesting that VvWRKY40 functions as a negative regulator in plant immunity.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Ana Cruz-Silva, Andreia Figueiredo, Monica Sebastiana
Summary: The study demonstrates that pre-inoculation of grapevine with AMF can alter the expression of P. viticola effectors, particularly PvRxLR28. This suggests that AMF may interfere with the pathogen's ability to infect grapevine by modulating the expression of pathogenicity effectors, supporting the hypothesis that AMF can be used to enhance plant resistance to pathogens and promote more sustainable agriculture practices.
Article
Plant Sciences
Ruiqi Liu, Tingting Chen, Xiao Yin, Gaoqing Xiang, Jing Peng, Qingqing Fu, Mengyuan Li, Boxing Shang, Hui Ma, Guotian Liu, Yuejin Wang, Yan Xu
Summary: Pathogens secrete effectors to manipulate host cells, such as Plasmopara viticola's RXLR31154 which interacts with the host protein VpPsbP to enhance colonization. Furthermore, VpPsbP in chloroplasts reduces H2O2 accumulation and activates the O-1(2) signaling pathway, promoting disease.
Article
Plant Sciences
Qingqing Fu, Yunlei Wang, Jing Yang, Yu Jiao, Wenjuan Li, Fan Yang, Xiao Yin, Boxing Shang, Ruiqi Liu, Yiyuan Zhang, Ayisuwake Saileke, Guotian Liu, Steven van Nocker, Huiling Hu, Yan Xu
Summary: The Plasmopara viticola effector PvAvh77 induces cell death, suppresses plant immunity intracellularly, and triggers responses extracellularly. It was observed that the PvAvh77 gene was strongly up-regulated during the initial stages of P. viticola infection in Vitis vinifera. PvAvh77 can induce non-specific cell death when expressed in Vitis riparia and in tobacco.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Tingting Chen, Jing Peng, Xiao Yin, Meijie Li, Gaoqing Xiang, Yuejin Wang, Yan Lei, Yan Xu
Summary: In this study, researchers identified the physical interaction between the RxLR effector PvAVH53 from the grapevine oomycete pathogen Plasmopara viticola and grapevine nuclear import factor importin alphas, which plays a crucial role in manipulating plant cellular immunity and inducing cell death. Silencing the expression of importin-alpha genes increased susceptibility to certain pathogens, highlighting the essential role of importin-alpha s in host innate immunity.
HORTICULTURE RESEARCH
(2021)
Article
Plant Sciences
Lorenzo Valleggi, Giuseppe Carella, Rita Perria, Laura Mugnai, Federico Mattia Stefanini
Summary: In this study, a Bayesian model was developed to predict the probability of Plasmopara viticola infection in grapevines, considering various disease management approaches. The results indicated that in situations of high disease pressure, the application of biostimulants alone emerged as the most effective strategy.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Zeraye Mehari Haile, Daniel Endale Gebremichael, Luca Capriotti, Barbara Molesini, Francesca Negrini, Marina Collina, Silvia Sabbadini, Bruno Mezzetti, Elena Baraldi
Summary: Downy mildew caused by Plasmopara viticola is a devastating disease for grapevine, and the application of exogenous double-stranded RNA targeting specific genes shows promise for disease management. The dsRNA not only affects the virulence of the pathogen, but also reduces the disease progress rate, providing a potential new generation of RNA-based resistant plants or agrochemicals for downy mildew disease management in grapevine.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Leah J. Kim, Kaitlyn M. Tsuyuki, Fengling Hu, Emily Y. Park, Jingwen Zhang, Jennifer G. Iraheta, Ju-Chen Chia, Rong Huang, Avery E. Tucker, Madeline Clyne, Claire Castellano, Angie Kim, Daniel D. Chung, Christopher T. DaVeiga, Elizabeth M. Parsons, Olena K. Vatamaniuk, Jeeyon Jeong
Summary: This study reveals the essential role of Arabidopsis ferroportin 3 (FPN3) in maintaining optimal iron homeostasis in mitochondria and chloroplasts. Lack of FPN3 leads to disrupted iron levels and abnormal ultrastructure in mitochondria, affecting plant growth under iron-deficient conditions.
Article
Biochemistry & Molecular Biology
Seungjin Woo, Byeongho Moon, Inhwan Hwang
Summary: The mechanism underlying mitochondrial targeting of the dual-targeting protein AtSufE1 in Arabidopsis was elucidated. It involves specific sequence motifs and the interaction with import receptors Metaxin and Tom20.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Evolutionary Biology
Clotilde Garrido, Francis-Andre Wollman, Ingrid Lafontaine
Summary: This study retraces the evolution and acquisition of mitochondrial and chloroplast processing peptidases, which play a crucial role in targeting peptide processing and degradation. The findings provide insights into the complex history of lateral gene transfers among bacteria and the involvement of bacteria in the establishment of proteolytic pathways in organelles. The study also highlights the conservation and divergence between bacterial and eukaryotic peptidase homologs, shedding light on their functional contexts.
GENOME BIOLOGY AND EVOLUTION
(2022)
Article
Materials Science, Biomaterials
Fangfang Meng, Junyi He, Jie Niu, Yawen Li, Peng Gao, Xiaoqiang Yu
Summary: In this study, a dual-targeting group strategy was proposed to construct a single fluorescent probe that can simultaneously and discriminatively visualize two organelles. As a proof of concept, a single fluorescent probe named LDER was designed and synthesized, which can bind to lipid droplets and endoplasmic reticulum evenly. Furthermore, the interaction between lipid droplets and endoplasmic reticulum was investigated.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Alexander N. Gasparski, Konstadinos Moissoglu, Sandeep Pallikkuth, Sezen Meydan, Nicholas R. Guydosh, Stavroula Mili
Summary: Numerous proteins can be targeted to different subcellular destinations, and this targeting is thought to be determined by protein sorting mechanisms. However, this study shows that mRNA location and translation rate can also influence protein targeting by regulating protein binding to specific partners. The location of protein synthesis and the rate of translation elongation act together to influence protein distribution and function, which can be modulated by physiological stimuli.
Article
Chemistry, Analytical
Xiaohuan Huang, Tiancheng Luo, Chuang Zhang, Junrong Li, Zejin Jia, Xulang Chen, Yanjun Hu, Hong Huang
Summary: Viscous microenvironments can lead to common diseases, but there are currently no effective methods for dual-function detection of reactive oxygen species and viscosity. This study introduces a dual-ratiometric-response probe for ClO- and viscosity detection, which shows promising potential for clinical assays.
Article
Pharmacology & Pharmacy
Veronika Talianova, Zdenek Kejik, Robert Kaplanek, Katerina Vesela, Nikita Abramenko, Lukas Lacina, Karolina Strnadova, Barbora Dvorankova, Pavel Martasek, Michal Masarik, Magdalena Houdova Megova, Petr Busek, Jana Krizova, Lucie Zdrazilova, Hana Hansikova, Erik Vlcak, Vlada Filimonenko, Aleksi Sedo, Karel Smetana, Milan Jakubek
Summary: IL-6 signaling plays a crucial role in the pathogenesis of serious diseases such as chronic inflammation and cancer. Two newly designed bis-pentamethinium salts compounds showed potential in binding IL-6 receptor and inhibiting mitochondrial metabolism in cancer cells.