Article
Infectious Diseases
Kevin Simon, Wolfgang Pier, Alex Kruettgen, Hans-Peter Horz
Summary: The combination of Sb-1 phage and oxacillin showed enhanced antibacterial effects against most S. aureus isolates, with rare antagonism observed. This heterologous antimicrobial pair provides a robust approach for controlling MRSA.
Article
Biochemistry & Molecular Biology
Jun Wang, Xinxin Ma, Jing Li, Lu Shi, Lijuan Liu, Xinyao Hou, Sijin Jiang, Pu Li, Jia Lv, Lei Han, Yue Cheng, Bei Han
Summary: It is imperative to explore alternative antimicrobial treatments for methicillin-resistant Staphylococcus aureus (MRSA) infections. This study found that a combination of Nisin (NIS) and Oxacillin (OX) can effectively inhibit MRSA and potentially restore its sensitivity to OX by inhibiting the transcription of mecA gene. This research has important clinical implications for developing new anti-MRSA biomaterials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Minjun Kim, Yena Seo, Seon-Gyeong Kim, Yedam Choi, Hyun Jung Kim, Tae-Jong Kim
Summary: Resistant bacteria pose a critical problem in bacterial infection treatment, and the development of new antibiotics is not the optimal solution. Combining safe, plant-based compounds with conventional antibiotics may be a more reasonable approach to increase efficacy against resistant bacteria. This study investigates the synergistic antibiotic activity of Ricini Semen extract and its constituent fatty acids when mixed with oxacillin, showing that changes in cell membrane fluidity play a major role in this mechanism.
Article
Nanoscience & Nanotechnology
Maricely Ramirez-Hernandez, Javiera Norambuena, Hongnan Hu, Belvin Thomas, Chaoyun Tang, Jeffrey M. Boyd, Tewodros Asefa
Summary: The urgent need for materials that can more efficiently deliver antimicrobial agents has arisen due to the increase in microbial resistance to traditional antibiotics. This study presents the synthesis of amine-functionalized SBA-15 mesoporous silica nanomaterials loaded with rafoxanide and anchored Cu(II) ions, which exhibit enhanced antimicrobial efficacy against pathogenic Staphylococcus aureus. The structurally characterized nanomaterials demonstrate 10 times the bactericidal action compared to nanomaterials loaded with rafoxanide only, and the synthetic sequence used in the production significantly impacts their bactericidal efficacy.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Infectious Diseases
Elisabetta Buommino, Simona De Marino, Martina Sciarretta, Marialuisa Piccolo, Carmen Festa, Maria Valeria D'Auria
Summary: Oxadiazole compounds showed high efficacy against Gram-positive MRSA and were able to restore antibiotic activity, making them promising for the treatment of MRSA infections.
Article
Pharmacology & Pharmacy
Mohadeseh Mohammadi Tabar, Moj Khaleghi, Elham Bidram, Atefeh Zarepour, Ali Zarrabi
Summary: Infectious diseases are the second leading cause of death worldwide, and nanotechnology provides promising approaches to tackle antibiotic resistance. This study synthesized a carbon-based nanoplatform and successfully loaded two ineffective antibiotics to enhance their antibacterial activity.
Article
Infectious Diseases
Sabrina Di Gregorio, Maria Sol Haim, Angela Maria Rosa Famiglietti, Jose Di Conza, Marta Mollerach
Summary: This study characterized isogenic strains of S. aureus with vancomycin resistance, both in vivo and in vitro, and found genetic changes associated with cell wall and phosphate metabolism, staphylococcal growth, and biofilm formation. These changes are not commonly associated with vancomycin resistance in S. aureus.
Article
Infectious Diseases
Elisabetta Buommino, Adriana Vollaro, Francesca P. Nocera, Francesca Lembo, Marina DellaGreca, Luisa De Martino, Maria R. Catania
Summary: Resin acid, specifically abietic acid, has been shown to exhibit significant antimicrobial activity against MRSP, increasing the susceptibility of MRSP strains to oxacillin. Additionally, it demonstrates a moderate antagonistic effect on the vitality of treated MSSP and MRSP biofilms, as well as reducing the expression of mecA, mecR1, and mec1 genes. These findings suggest the potential of abietic acid as a therapeutic agent in combinatorial antibiotic therapy against MRSP.
Review
Infectious Diseases
Harshad Lade, Jae-Seok Kim
Summary: MRSA is a prevalent bacterial pathogen worldwide, and finding new antibiotic targets is crucial for successful treatment. Most antibiotics target essential biochemical processes of S. aureus, such as cell wall synthesis. Further research into novel antibiotics targeting bacterial cellular processes could lead to new therapeutic strategies against antibiotic-resistant pathogens.
Article
Biotechnology & Applied Microbiology
Mohammed H. Alqarni, Ahmed Foudah, Aftab Alam, Mohammad A. Salkini, Magdy M. Muharram, Nikolaos E. Labrou, Pinki Rawat
Summary: Bacterial infections caused by antibiotic-resistant pathogens are a significant public health problem. Develop innovative strategies to effectively eliminate antibiotic-resistant microorganisms are urgently needed. Coumarin has shown significant effectiveness against MRSA and can be used as an alternative treatment. Nanoparticle-based targeted therapies can effectively treat MRSA infections.
BIOENGINEERING-BASEL
(2022)
Article
Microbiology
Sanjam S. Sawhney, Eric M. Ransom, Meghan A. Wallace, Patrick J. Reich, Gautam Dantas, Carey-Ann D. Burnham
Summary: This study discovered a high prevalence of Staphylococcus aureus isolates with a borderline oxacillin resistance phenotype (BORSA) in the neonatal intensive care unit (NICU). By analyzing mutations and truncations and using a random forest classification model, BORSA and methicillin-susceptible S. aureus were distinguished among clinical isolates collected from two continents. This work highlights the need for improved MRSA screening methods and the presence of the non-mecA-mediated BORSA phenotype.
Article
Microbiology
Sanjam S. Sawhney, Eric M. Ransom, Meghan A. Wallace, Patrick J. Reich, Gautam Dantas, Carey-Ann D. Burnham
Summary: In this study, researchers discovered a high prevalence of Staphylococcus aureus isolates exhibiting a borderline oxacillin resistance phenotype (BORSA) in their neonatal intensive care unit (NICU). They found that the current MRSA screening methods misclassified BORSA, and identified specific markers that can distinguish BORSA from methicillin-susceptible S. aureus (MSSA). The findings have implications for epidemiological reporting of MRSA rates and can help improve MRSA screening methods.
Article
Infectious Diseases
Ramona Iseppi, Carla Condo, Patrizia Messi
Summary: Research suggests that Melaleuca alternifolia and Eucalyptus globulus essential oils have antimicrobial activity against methicillin-resistant Staphylococcus aureus. By altering the membrane permeability and in combination with oxacillin or other essential oils, the bacterial load can be reduced.
Review
Materials Science, Multidisciplinary
Shreya Kanth, Akshatha Nagaraja, Yashoda Malgar Puttaiahgowda
Summary: The current global death rate is threatened by deadly unknown infections and the emergence of multidrug-resistant bacteria. Combatting drug-resistant bacteria, such as MRSA, has become a daunting challenge globally, with polymer synthesis being explored as a significant approach in preventing the spread of infections.
JOURNAL OF MATERIALS SCIENCE
(2021)
Article
Cell Biology
Zhimin Bai, Min Chen, Qiaofa Lin, Ying Ye, Hongmei Fan, Kaizhen Wen, Jianxing Zeng, Donghong Huang, Wenfei Mo, Ying Lei, Zhijun Liao
Summary: By extracting feature vectors from protein sequences and using classification tools, MRSA and MSSA were successfully distinguished. MRSA showed high resistance to penicillin and high pathogenic risk. Cross-infection between different types of MRSA was found in Quanzhou, and MRSA in traditional hospitals exhibited increasingly blurred molecular characteristics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Infectious Diseases
Shih-Hao Lo, Chieh Chin, Po-Liang Lu, Shang-Yi Lin
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Infectious Diseases
Szu-Wei Huang, Mei-Chen Shen, Wen-Hung Wang, Wei-You Li, Jen-Hsien Wang, Cheng-Yin Tseng, Po-Yu Liu, Lih-Shinn Wang, Yu-Lin Lee, Yi-Ming Arthur Chen, Chun-Yuan Lee, Po-Liang Lu, Sheng-Fan Wang
Summary: The prevalence of TDR among HIV-1-infected patients in Taiwan is increasing, with NNRTI-related mutations being the most common. A higher baseline HIV-1 viral load and receiving InSTI-based regimens are associated with shorter time to virological failure or longer time to viral suppression in this population.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Immunology
Yoon Bin Chong, Po-Liang Lu, Yu-Chun Ma, Hsin-Ling Yin, Chih-Hui Chang
Summary: Epstein-Barr virus-associated smooth muscle tumor (EBV-SMT) is a rare tumor found in immunocompromised patients, and its treatment is not well-established. This study presents the first case, to the authors' knowledge, of a tumor that shrank in association with elevated CD4 counts, highlighting the importance of the immune response in HIV-related EBV-SMT.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Chao-Ju Chen, Po-Liang Lu, Shu-Huei Jian, Hsiao-Ling Fu, Po-Hao Huang, Chung-Yu Chang
Summary: The Enterobacter cloacae complex (ECC) has emerged as a significant cause of nosocomial infections, especially with carbapenem-nonsusceptible isolates posing a threat to public health. This study identified the most commonly isolated species was E. hormaechei subsp. hoffmannii, with different species of CnsECC showing heterogeneity in resistance patterns. Carbapenemase-producing CnsECC infections were associated with poorer outcomes, with risk factors including exposure to carbapenems, chronic pulmonary disease, and ventilator support. The findings emphasize the importance of understanding resistance mechanisms to guide antibiotic treatment decisions.
Article
Biochemistry & Molecular Biology
Hung Su, Zong-Han Jiang, Shu-Fen Chiou, Jentaie Shiea, Deng-Chyang Wu, Sung-Pin Tseng, Shu-Huei Jain, Chung-Yu Chang, Po-Liang Lu
Summary: An analytical platform combining probe sampling, thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS), and multivariate statistical analysis was developed to rapidly differentiate bacterial species based on the differences in their lipid profiles. The results were validated by protein fingerprinting using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF). The developed platform showed promising potential as a useful diagnostic tool for rapidly distinguishing bacterial species.
Article
Engineering, Chemical
Md Khalid Anwer, Essam A. Ali, Muzaffar Iqbal, Mohammed Muqtader Ahmed, Mohammed F. Aldawsari, Ahmed Al Saqr, Ahmed Alalaiwe, Gamal A. Soliman
Summary: In this study, OLP-loaded PLGA nanoparticles and OLP-CS-PLGA nanoparticles were successfully prepared to improve the dissolution rate and bioavailability of Olaparib. The results showed that OLP-CS-PLGA nanoparticles could be an effective approach for enhancing the bioavailability of Olaparib.
Review
Biochemistry & Molecular Biology
Shih-Chun Yang, Ahmed Alalaiwe, Zih-Chan Lin, Yu-Chih Lin, Ibrahim A. Aljuffali, Jia-You Fang
Summary: miRNA plays a critical role in regulating skin inflammation and has anti-inflammatory features. miRNA-based therapies could be potential candidates for treating skin inflammation.
Article
Materials Science, Multidisciplinary
Mohammad Javed Ansari, Najeeb Ur Rehman, Elmoatasim Ibnouf, Ahmed Alalaiwe, Majid Ahmad Ganaie, Ameeduzzafar Zafar
Summary: This research article presents the development, characterization, stability, antimicrobial activity, antispasmodic activity, and antidiarrheal activity of silver nanoparticles synthesized and stabilized by a polymeric coating of gum tragacanth solution. The study found that the silver nanoparticles exhibited good stability and significant antimicrobial, antispasmodic, and antidiarrheal activities.
Article
Infectious Diseases
Chun-Yuan Lee, Chen-Hsiang Lee, Hung-Jen Tang, Hung-Chin Tsai, Chen-Hsun Yang, Yi-Pei Lin, Sheng-Fan Wang, Po-Liang Lu
Summary: This study found that treatment regimens with DTG/ABC/3TC were more likely to be discontinued in advanced HIV patients compared to B/F/TAF, but there were no significant differences in viral suppression and virological failure between the two regimens. Therefore, individualized treatment approaches are crucial for advanced HIV patients.
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Pharmacology & Pharmacy
Ahmed Alalaiwe, Mohammad A. Alsenaidy, Ziyad S. Almalki, Mohamed H. Fayed
Summary: This study developed and optimized sildenafil orodispersible mini-tablets (ODMTs) for the treatment of pediatric pulmonary hypertension. Microcrystalline cellulose and partially pre-gelatinized starch were found to significantly influence the quality attributes of sildenafil ODMTs. By optimizing the formulation, the study achieved the optimal sildenafil ODMTs that meet the USP acceptance criteria.
Correction
Infectious Diseases
Chun-Yuan Lee, Chen-Hsiang Lee, Hung-Jen Tang, Hung-Chin Tsai, Chen-Hsun Yang, Yi-Pei Lin, Sheng-Fan Wang, Po-Liang Lu
INFECTIOUS DISEASES AND THERAPY
(2023)
Article
Immunology
Tsung-Ying Yang, Wei-Chun Hung, Tsung-Han Tsai, Po-Liang Lu, Sheng-Fan Wang, Liang-Chun Wang, Yu-Tzu Lin, Sung-Pin Tseng
Summary: This study revealed the in vitro activities, mechanisms, and in vivo study of AS101 against carbapenemase-producing E. coli (CPEC). Our finding provided further evidence for the antibiotic development of AS101.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Immunology
Chia-Hui Wen, Po-Liang Lu, Chun-Yu Lin, Yi-Pei Lin, Tun-Chieh Chen, Yen-Hsu Chen, Shin-Huei Kuo, Shih-Hao Lo, Shang-Yi Lin, Chung-Hao Huang, Ya-Ting Chang, Chun-Yuan Lee
Summary: This study assessed the association between immunological nonresponses and incident non-AIDS-related events among people living with HIV.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2023)
Article
Dermatology
Wei-Huan Hu, Shang-Yi Lin, Yuh-Jyh Hu, Ho-Yin Huang, Po-Liang Lu
Summary: Machine learning models show potential for predicting mortality among patients with candidemia compared to clinical severity scores.
Article
Biochemistry & Molecular Biology
Zih-Syuan Yang, Wen-Hung Wang, Yu-Ting Lin, Chih-Yen Lin, Aspiro Nayim Urbina, Arunee Thitithanyanont, Po-Liang Lu, Yen-Hsu Chen, Sheng-Fan Wang
Summary: This study investigates the individual and collaborative roles of DC-SIGN and Gal3 in H5N1 and H7N9 avian influenza virus (AIV) infection. It is found that DC-SIGN expression significantly promotes viral infection, while Gal3 knockdown reduces viral infection. The co-expression of DC-SIGN and Gal3 enhances infectivity of H5N1 and H7N9 viruses, with stronger regulatory capabilities for H5N1. The study reveals the significance of DC-SIGN and Gal3 in regulating AIV infection and suggests the potential of targeting their interaction with HA protein as antiviral strategies.