Review
Biochemistry & Molecular Biology
Moaz Ahmad, Sasirekha Narayanasamy, Hwei Ling Ong, Indu Ambudkar
Summary: ER-PM junctions, formed by the endoplasmic reticulum and plasma membrane, play a crucial role in assembling calcium signaling complexes. The formation and stability of these junctions rely on the enrichment of specific proteins, which contribute to junctional stability and regulate the lipid levels of ER and plasma membranes.
Review
Cell Biology
Mahlegha Ghavami, Gregory D. Fairn
Summary: This minireview discusses the functions of ER-phagosome contact sites in the process of phagocytosis, from the early stages of particle engulfment to the dissolution of phagolysosome into lysosomes. It also highlights the aspects of ER-phagosome contact sites that require further exploration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Bailey Hewlett, Neha Pratap Singh, Christian Vannier, Thierry Galli
Summary: Membrane contact sites play crucial roles in cell differentiation, plasticity and maintenance, and dysfunction can lead to pathological conditions. Each membrane contact site displays a unique molecular signature, adapted to the functions of different organelles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Nicholas C. Vierra, Samantha C. O'Dwyer, Collin Matsumoto, L. Fernando Santana, James S. Trimmer
Summary: In mammalian brain neurons, membrane depolarization triggers Ca2+ influx through voltage-gated Ca2+ channels, leading to diverse cellular responses, including gene expression. The L-type Ca2+ channels in neuronal soma and dendrites play a key role in this process. The organization of signaling complexes by Kv2.1 at endoplasmic reticulum-plasma membrane junctions enhances the clustering and activity of Cav1.2 channels, impacting somatic Ca2+ signals and excitation-transcription coupling.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Sarah D. Neuman, Jeff R. Jorgensen, Amy T. Cavanagh, Jeremy T. Smyth, Jane E. Selegue, Scott D. Emr, Arash Bashirullah
Summary: Membrane contact sites play crucial roles in organelle signaling and communication. This study reveals a novel role for the Hobbit proteins at endoplasmic reticulum-plasma membrane (ER-PM) contact sites, demonstrating their localization and interaction with plasma membrane phosphatidylinositols. Furthermore, the essentiality of Hobbit protein in Drosophila development makes it one of the first examples of a membrane contact site-localized lipid binding protein required for viability.
JOURNAL OF CELL SCIENCE
(2022)
Article
Clinical Neurology
Dajana Grossmann, Nina Malburg, Hannes Glass, Veronika Weeren, Verena Sondermann, Julia F. F. Pfeiffer, Janine Petters, Jan Lukas, Philip Seibler, Christine Klein, Anne Gruenewald, Andreas Hermann
Summary: Recent studies have shown that PINK1 and Parkin play a crucial role in mitochondrial calcium homeostasis and regulation of MERCSs. Using iPSC-derived dopaminergic neurons, researchers found that deficiency in PINK1 or PRKN affects MERCSs dynamics and composition, leading to impaired calcium homeostasis. These findings provide insights into the pathogenesis of PINK1/Parkin-related Parkinson's disease.
MOVEMENT DISORDERS
(2023)
Review
Cell Biology
Saeko Aoyama-Ishiwatari, Yusuke Hirabayashi
Summary: Recent studies have shown that organelles do not exist in isolation, but form complex cooperative networks. Mitochondria-endoplasmic reticulum contact sites play crucial roles in regulating various biochemical and signaling functions within cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Yelena Sargsyan, Uta Bickmeyer, Christine S. Gibhardt, Katrin Streckfuss-Bomeke, Ivan Bogeski, Sven Thoms
Summary: Peroxisomes in HeLa cells and cardiomyocytes are able to uptake calcium and are tightly interconnected with cytosolic calcium signals. Cardiac peroxisomes can uptake calcium on a beat-to-beat basis, indicating a potential role in shaping cellular calcium dynamics of cardiomyocytes.
LIFE SCIENCE ALLIANCE
(2021)
Article
Oncology
Shuwen Han, Yinhang Wu, Jing Zhuang, Qiang Yan, Yizhen Jiang, Quan Qi, Yin Jin, Jiang Liu, Xi Yang
Summary: High cholesterol levels can induce CD8(+) T cell exhaustion, leading to immune escape in colorectal cancer (CRC). Hypercholesterolemia damages the endoplasmic reticulum (ER) structure of CD8(+) T cells and increases the expression of ER stress molecules, causing CD8(+) T cell exhaustion. In CD8(+) T cells, high cholesterol levels increase the expression of mitophagy-related proteins, leading to mitochondrial energy disturbance. High cholesterol enhances the colocalization of ER-mitochondria contact site (ERMC) proteins, indicating an interaction between ER and mitochondrial membranes in CD8(+) T cells.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Immunology
Qiangqiang Shi, Qiang Wang, Li Liu, Junjie Chen, Beibei Wang, Saverio Bellusci, Chengshui Chen, Nian Dong
Summary: This study investigates the regulatory mechanism of FGF10 on ER stress in PM-induced lung injury. The findings show that FGF10 treatment attenuates PM-induced lung injury and reverses the levels of ER stress proteins. Additionally, FGF10 promotes cell proliferation and restrains the secretion of inflammatory cytokines.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Cell Biology
Laura Lopez-Molina, Joaquin Fernandez-Irigoyen, Carmen Cifuentes-Diaz, Jordi Alberch, Jean-Antoine Girault, Enrique Santamaria, Silvia Gines, Albert Giralt
Summary: This study explores the function of Pyk2 in mitochondria-associated membranes (MAMs) and demonstrates its role in regulating ER-mitochondria calcium efflux, affecting mitochondrial function and leading to calcium homeostasis imbalance.
Article
Biochemical Research Methods
Tito Cali, Marisa Brini
Summary: Membrane contact sites between organelles play a crucial role in cellular homeostasis and can be quantified using SPLICS sensors in both fixed and living cells.
Correction
Biochemistry & Molecular Biology
Emma Louise Wilson, Emmanouil Metzakopian
Summary: A correction to this paper has been published.
CELL DEATH AND DIFFERENTIATION
(2021)
Review
Cell Biology
Aurora Gil-Hernandez, Miguel Arroyo-Campuzano, Arturo Simoni-Nieves, Cecilia Zazueta, Luis Enrique Gomez-Quiroz, Alejandro Silva-Palacios
Summary: Membrane contact sites (MCS) are defined as areas of proximity between heterologous or homologous membranes characterized by specific proteins, playing a crucial role in regulating physiological processes such as apoptosis, calcium, and lipid signaling. Recent studies have highlighted the significance of MCS in cancer cells, particularly in calcium and lipid signaling, as well as their role in tumor progression, which may have implications for cancer biology research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Maria Livia Sassano, Blanca Felipe-Abrio, Patrizia Agostinis
Summary: Membrane contact sites (MCS) between organelles are crucial for the structural integrity and homeostasis of eukaryotic cells, facilitating intracellular signaling and exchange of ions, metabolites, and lipids. In particular, ER-mitochondria contacts (EMCS) play a key role in regulating lipid composition, physiological functions, and lipid-mediated signaling pathways, with disruptions potentially favoring key traits of cancer cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
