Article
Biotechnology & Applied Microbiology
Istvan Nagy, Elisabeth Veeckman, Chang Liu, Michiel Van Bel, Klaas Vandepoele, Christian Sig Jensen, Tom Ruttink, Torben Asp
Summary: In this study, a chromosome-scale genome sequence assembly of perennial ryegrass was reported, which will serve as valuable resources for evolutionary and comparative genomics as well as breeding applications.
Article
Plant Sciences
Daniel Grogg, Marius Rohner, Steven Yates, Chloe Manzanares, Simon E. Bull, Sue Dalton, Maurice Bosch, Bruno Studer, Giovanni A. L. Broggini
Summary: This study reported the induction of callus from various genotypes and explants of perennial ryegrass, and achieved gene silencing and gene knockout through plasmid transformation. This research is of great significance for expanding the range of transformable genotypes and improving forage and turf grass crops.
Article
Plant Sciences
Kim A. Richardson, Anouck C. M. de Bonth, Zac Beechey-Gradwell, Suhas Kadam, Luke J. Cooney, Kelly A. Nelson, Ruth Cookson, Somrutai Winichayakul, Michele Reid, Philip Anderson, Tracey Crowther, Xiuying Zou, Dorothy Maher, Hong Xue, Richard W. Scott, Anne Allan, Richard D. Johnson, Stuart D. Card, Wade J. Mace, Nicholas J. Roberts, Gregory Bryan
Summary: The study investigated the impact of DGAT + CO technology on the grass-endophyte association in perennial ryegrass and found that the expression of this trait did not significantly affect the endophyte-infected and non-infected progeny. It was also observed that while Epichloe-derived alkaloid concentrations were lower in DGAT + CO plants in some instances, they remained within the bioactive range. Further research is needed to determine the optimal genetic backgrounds for the successful integration of DGAT + CO trait with selected endophyte strains.
Article
Plant Sciences
Vincent Colas, Philippe Barre, Frederik van Parijs, Lukas Wolters, Yannick Quitte, Tom Ruttink, Isabel Roldan-Ruiz, Abraham J. Escobar Gutierrez, Hilde Muylle
Summary: This study investigated the forage digestibility of perennial ryegrass in two important cutting periods and found that the season and genetic factors have an impact on forage quality. It also identified the influence of cell wall composition on digestibility, such as the effect of hemicellulose concentration on spring digestibility and lignin concentration on autumn digestibility.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biology
Weihu Lin, Chengfen Gao, Jianjun Wang, Wenbo Xu, Meining Wang, Miaomiao Li, Bihua Ma, Pei Tian
Summary: Drought stress increases the concentration of peramine in perennial ryegrass, but does not affect the concentration of lolitrem B. It also reduces plant height and dry matter content.
Article
Plant Sciences
Carl A. Frisk, Georgianna Xistris-Songpanya, Matthieu Osborne, Yastika Biswas, Rainer Melzer, Jon M. Yearsley
Summary: Identifying the impact of climate change components on ecosystems and vegetation is crucial. Waterlogging induced by climate change has been understudied. Long-term waterlogging was found to reduce leaf-color intensity and affect the performance and yield of perennial ryegrass. Climate change treatment further reduced color intensities. Flooding reduced plant productivity via reductions in color pigments and root proliferation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Sebastian W. Przemieniecki, Cezary Purwin, Jedrzej Mastalerz, Marta Borsuk, Krzysztof Lipinski, Tomasz Kurowski
Summary: The study found that a dose of 5 kg ha(-1) of L-tryptophan significantly increased herbage yield and water-soluble carbohydrate content, leading to improved fermentation process and reduced levels of harmful bacteria and fungi. Overall, using L-tryptophan at the appropriate dose can enhance nutrient consumption by Lolium perenne and improve the quality of its silage as feed.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Zongchao Liao, Hossein Ghanizadeh, Xin Zhang, Hechuan Yang, Ying Zhou, Linkai Huang, Xinquan Zhang, Yiwei Jiang, Gang Nie
Summary: In this study, the miRNA expression profiles of perennial ryegrass were analyzed to uncover the regulatory mechanisms involved in heat tolerance mediated by exogenously applied methyl jasmonate (MeJA). The results showed that 102 miRNAs were up-regulated in all treatments, with different numbers of miRNAs being up-regulated in the MeJA pre-treatment, heat stress treatment, and MeJA pre-treatment + heat stress groups. Co-expression network analysis revealed that 20 miRNAs modulated 51 potential target genes, with specific pathways related to heat tolerance being influenced by MeJA. Overall, this research provides a foundation for further understanding the heat tolerance mechanism in perennial ryegrass and developing cultivars with enhanced heat tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Lea Deinert, Israel Ikoyi, Bastian Egeter, Patrick Forrestal, Achim Schmalenberger
Summary: Various nutrient recycling technologies are being developed to reduce the dependence on non-renewable raw materials for the production of phosphorus fertilizers in agriculture. This study investigated the effects of different phosphorus fertilizers on plant growth and soil microbiota, and found that recycling-derived fertilizers from agricultural waste can be a suitable alternative to traditional superphosphate fertilization.
Article
Plant Sciences
Wagdi Ghaleb, Philippe Barre, Beatrice Teulat, Lina Qadir Ahmed, Abraham J. Escobar-Gutierrez
Summary: Seed dormancy and inhibition are adaptive mechanisms that ensure seedlings are established at the most favorable time and place. This study investigated the genetic control and selective breeding potential of thermal requirements for germination in perennial ryegrass. The results showed that thermoinhibition at 10 and 32 degrees C in a natural population of perennial ryegrass has a genetic basis, with a single recessive gene involved at 10 degrees C.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Simone Vassiliadis, Priyanka Reddy, Joanne Hemsworth, German C. Spangenberg, Kathryn M. Guthridge, Simone J. Rochfort
Summary: Perennial ryegrass commonly infected with asexual Epichloe species endophytes produces alkaloids that provide enhanced bioprotection. However, the distribution of alkaloids within the plant and their role in biocontrol of insect pests and diseases are not well understood. This study used LC-MS to measure alkaloid concentrations in different tissues, revealing that concentrations were generally highest in seeds but varied in shoots/roots. Understanding alkaloid distribution may assist in determining resistance to subterranean root-feeding pests.
Article
Plant Sciences
Gang Nie, Jie Zhou, Yiwei Jiang, Jie He, Yang Wang, Zongchao Liao, Charlotte Appiah, Dandan Li, Guangyan Feng, Linkai Huang, Xia Wang, Xinquan Zhang
Summary: This study investigated the transcriptomic mechanisms underlying heat tolerance mediated by exogenous MeJA in perennial ryegrass. The results showed that exogenous MeJA improved the heat tolerance by mediating the expression of genes involved in different pathways, such as chlorophyll biosynthesis and degradation, antioxidant enzyme system, HSF-HSP network, and JAs biosynthesis.
Review
Biotechnology & Applied Microbiology
Madhu Patial, Ruchi Chauhan, Harinder K. Chaudhary, Kallol K. Pramanick, Arun K. Shukla, Vishnu Kumar, Ramesh P. S. Verma
Summary: The development and improvement of biotechnological tools have greatly contributed to the progress in barley breeding program. The use of doubled haploids has significantly shortened the cultivar development time and has been widely used in various genetic studies. However, challenges such as low efficiency and genotypic specificity still need to be addressed.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Jia Tang, Manli Li, Peisheng Mao, Yiwei Jiang
Summary: The study found that GABA can enhance seed germination, seedling growth, and improve the tolerance of perennial ryegrass to salinity stress by altering carbohydrate and antioxidant metabolism.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Plant Sciences
Juanjuan Fu, Shentong Zhang, Huina Jiang, Xinfei Zhang, Hao Gao, Peizhi Yang, Tianming Hu
Summary: This study reveals that melatonin enhances cold and drought tolerance in perennial ryegrass by activating antioxidant systems and reducing H2O2 accumulation. The expression of brassinosteroids biosynthesis and signaling-related genes is regulated by melatonin. The interplay between melatonin, H2O2, and brassinosteroid signaling plays a crucial role in melatonin-induced cold/drought tolerance in perennial ryegrass.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Miguel Loera-Sanchez, Bruno Studer, Roland Kolliker
Summary: Grasslands are important ecosystems for sustainable roughage production, and plant genetic diversity plays a crucial role in their functioning. The advancement of high-throughput DNA sequencing approaches provides opportunities for multispecies plant genetic diversity monitoring in grasslands.
MOLECULAR ECOLOGY RESOURCES
(2022)
Article
Plant Sciences
Michaela Jung, Beat Keller, Morgane Roth, Maria Jose Aranzana, Annemarie Auwerkerken, Walter Guerra, Mehdi Al-Rifai, Mariusz Lewandowski, Nadia Sanin, Marijn Rymenants, Frederique Didelot, Christian Dujak, Carolina Font i Forcada, Andrea Knauf, Francois Laurens, Bruno Studer, Helene Muranty, Andrea Patocchi
Summary: The study using the multi-environment apple reference population demonstrated genetic architecture and genomic predictive abilities for 30 quantitative traits in apple; GWAS revealed 59 stable and 277 location-specific associations, 69.2% of which were novel; genotype by environment interactions accounted for up to 24% of the phenotypic variability.
HORTICULTURE RESEARCH
(2022)
Article
Plant Sciences
Daniel Ariza-Suarez, Beat Keller, Anna Spescha, Johan Steven Aparicio, Victor Mayor, Ana Elizabeth Portilla-Benavides, Hector Fabio Buendia, Juan Miguel Bueno, Bruno Studer, Bodo Raatz
Summary: This study identifies the resistance locus BLC7.1 against Bean leaf crumple virus (BLCrV) in common bean and provides novel diagnostic markers for marker-assisted selection. The study highlights the potential of previous breeding efforts for virus resistance in accumulating positive alleles against newly emerging viruses.
Editorial Material
Ecology
Giulio Formenti, Kathrin Theissinger, Carlos Fernandes, Iliana Bista, Aureliano Bombarely, Christoph Bleidorn, Claudio Ciofi, Angelica Crottini, Jose A. Godoy, Jacob Hoglund, Joanna Malukiewicz, Alice Mouton, Rebekah A. Oomen, Sadye Paez, Per J. Palsboll, Christophe Pampoulie, Maria J. Ruiz-Lopez, Hannes Svardal, Constantina Theofanopoulou, Jan de Vries, Ann-Marie Waldvogel, Guojie Zhang, Camila J. Mazzoni, Erich D. Jarvis, Miklos Balint
Summary: Progress in genome sequencing has enabled the generation of large-scale reference genomes, representing global biodiversity. These genomes provide unique insights into genomic diversity and architecture, allowing comprehensive analyses in population and functional genomics, and are expected to revolutionize conservation genomics.
TRENDS IN ECOLOGY & EVOLUTION
(2022)
Article
Microbiology
Florian Goettelmann, Veronica Roman-Reyna, Sebastien Cunnac, Jonathan M. Jacobs, Claude Bragard, Bruno Studer, Ralf Koebnik, Roland Koelliker
Summary: By sequencing the genomes of 10 Xanthomonas translucens pathotype strains, researchers identified 3 distinct clades and strain-specific effectors, which could be crucial for pathogenicity and host adaptation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Beat Keller, Daniel Ariza-Suarez, Ana Elisabeth Portilla-Benavides, Hector Fabio Buendia, Johan Steven Aparicio, Winnyfred Amongi, Julius Mbiu, Susan Nchimbi Msolla, Phillip Miklas, Timothy G. Porch, James Burridge, Clare Mukankusi, Bruno Studer, Bodo Raatz
Summary: The common bean has a high diversity of growth type, pod and seed characteristics due to its two major origins of domestication. The climbing growth habit is associated with various traits, including flowering time, seed iron concentration, nitrogen fixation, and yield. This study identified several marker-trait associations and candidate genes related to growth habit and seed characteristics. Additionally, including bush-type bean lines in the training population improved the accuracy of genomic predictions for seed iron concentration and yield in climbing beans.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Alessia Russo, Baptiste Mayjonade, Daniel Frei, Giacomo Potente, Roman T. Kellenberger, Lea Frachon, Dario Copetti, Bruno Studer, Juerg E. Frey, Ueli Grossniklaus, Philipp M. Schluter
Summary: This article describes a method for extracting high molecular weight (HMW) DNA from low input plant material, which is easy to follow and quick. It successfully enables HMW DNA extraction from different plant species and is suitable for long-read sequencing on the Oxford Nanopore Technologies PromethION(R) platform.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Ina Schlathoelter, Michael Meissle, Timea Boeriis, Dominique Heimo, Bruno Studer, Giovanni A. L. Broggini, Jorg Romeis, Andrea Patocchi
Summary: Genetic modification through cisgenesis allows for the quick introduction of traits into apple cultivars without crossing. The cisgenic apple line C44.4.146, carrying the fire blight resistance gene FB_MR5, was generated using this approach. In this study, major leaf components and the effects on two decomposers were assessed to determine any unintended effects of the genetically modified apple. The results showed that the genetic modification did not alter major leaf components and did not negatively affect the survival, growth, or fecundity of the decomposers. The differences between conventionally bred cultivars were greater than between the genetically modified line and its non-genetically modified wild type.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Daniel Grogg, Marius Rohner, Steven Yates, Chloe Manzanares, Simon E. Bull, Sue Dalton, Maurice Bosch, Bruno Studer, Giovanni A. L. Broggini
Summary: This study reported the induction of callus from various genotypes and explants of perennial ryegrass, and achieved gene silencing and gene knockout through plasmid transformation. This research is of great significance for expanding the range of transformable genotypes and improving forage and turf grass crops.
Article
Agronomy
Lea A. Frey, Tim Vleugels, Tom Ruttink, Franz X. Schubiger, Marie Pegard, Leif Skot, Christoph Grieder, Bruno Studer, Isabel Roldan-Ruiz, Roland Koelliker
Summary: This study investigated the variability and candidate loci associated with resistance to southern anthracnose and clover rot in a worldwide collection of red clover. The findings provide valuable insights for genomics-assisted breeding and the development of novel cultivars with increased resistance against these devastating diseases.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Marius Rohner, Chloe Manzanares, Steven Yates, Daniel Thorogood, Dario Copetti, Thomas Luebberstedt, Torben Asp, Bruno Studer
Summary: Self-incompatibility (SI) is a genetic mechanism in plants that prevents inbreeding after self-pollination. Researchers have identified two genes, ZDUF247-I and ZDUF247-II, as well as the gene sZ, which determine pollen and stigma components in perennial ryegrass. The genes for SI in ryegrass show similar composition and structure to those in the S-locus, supporting the hypothesis of a duplicated origin of the two-locus SI system in grasses. Comparative genomic analyses revealed that the absence of functional copies of SI determinants leads to self-compatibility. This study provides insights into the origin and evolution of the unique gametophytic SI system in grasses, which is economically important.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Plant Sciences
Joanna Majka, Marek Glombik, Alzbeta Dolezalova, Jana Knerova, Marco Tulio Mendes Ferreira, Zbigniew Zwierzykowski, Martin Duchoslav, Bruno Studer, Jaroslav Dolezel, Jan Bartos, David Kopecky
Summary: We analyzed the chromosome composition of interspecific plant hybrids and found that the transmission of parental genomes varied in male and female meiosis. Festuca chromosomes were replaced by Lolium counterparts in female meiosis, while in male meiosis, Festuca chromosomes showed improper attachment and were gradually eliminated. The difference in behavior may be attributed to non-synonymous mutations and the exclusive expression of kinetochore proteins from the Lolium allele.
Article
Plant Sciences
Ina Schlatholter, Giovanni A. L. Broggini, Sebastian Streb, Bruno Studer, Andrea Patocchi
Summary: Cisgenesis was used to introduce fire blight resistance to the apple cultivar 'Gala Galaxy' without affecting other tree-, flower-, or fruit-related traits. A 5-year field trial showed no significant differences between the cisgenic line C44.4.146 and control genotypes in terms of tree-, flower-, or fruit-related traits. However, fruits of C44.4.146 and its wild-type 'Gala Galaxy' were paler in color compared to non-tissue cultured 'Gala Galaxy' fruits.
Article
Multidisciplinary Sciences
Chun Yue Wei, Steven Yates, Dan Zhu, Andreas Hund, Bruno Studer, Laura Nystrom
Summary: This study evaluated the genetic association of lipase and esterase activities in wholegrain wheat flour of 300 European wheat cultivars harvested in 2015 and 2016. The results showed significant variations in enzyme activities among different cultivars, with a large environmental impact. Cultivars 'Julius' and 'Bueno' were suggested to be better suited for stable wholegrain products due to consistently low enzyme activities. Genome-wide association study identified candidate genes associated with esterase and lipase activity in wholegrain flour, offering new opportunities to improve lipid stability of wholegrain wheat.
Review
Genetics & Heredity
Kathrin Theissinger, Carlos Fernandes, Giulio Formenti, Iliana Bista, Paul R. Berg, Christoph Bleidorn, Aureliano Bombarely, Angelica Crottini, Guido R. Gallo, Jose A. Godoy, Sissel Jentoft, Joanna Malukiewicz, Alice Mouton, Rebekah A. Oomen, Sadye Paez, Per J. Palsboll, Christophe Pampoulie, Maria J. Ruiz-Lopez, Simona Secomandi, Hannes Svardal, Constantina Theofanopoulou, Jan de Vries, Ann-Marie Waldvogel, Guojie Zhang, Erich D. Jarvis, Miklos Balint, Claudio Ciofi, Robert M. Waterhouse, Camila J. Mazzoni, Jacob Hoglund
Summary: The availability of public genomic resources can greatly assist biodiversity assessment, conservation, and restoration efforts. Reference genomes play a key role in facilitating biodiversity research and conservation. Integrating the use of reference genomes as a best practice in conservation genomics is essential.
TRENDS IN GENETICS
(2023)