Review
Cell Biology
Yan Man, Xiangmei Yao, Tonghua Yang, Yajie Wang
Summary: The self-renewal and differentiation of hematopoietic stem cells are tightly controlled by various cells and cytokines in the bone marrow microenvironment. Changes in the niche composition can lead to hematological malignancies, and processes like homing, proliferation, and differentiation of HSCs are crucial for the success of hematopoietic stem cell transplantation. Single-cell sequencing and in vivo imaging are powerful tools for studying the bone marrow microenvironment in diseases like leukemia and after transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ningbo Wu, Hongxiang Sun, Xiaoyun Zhao, Yao Zhang, Jianmei Tan, Yuanyuan Qi, Qun Wang, Melissa Ng, Zhaoyuan Liu, Lingjuan He, Xiaoyin Niu, Lei Chen, Zhiduo Liu, Hua-Bing Li, Yi Arial Zeng, Manolis Roulis, Dou Liu, Jinke Cheng, Bin Zhou, Lai Guan Ng, Duowu Zou, Youqiong Ye, Richard A. Flavell, Florent Ginhoux, Bing Su
Summary: The study identified a subset of intestinal stromal cells, MRISCs, as the primary cellular source of WNT agonist R-spondin 1 following intestinal injury in mice. These MRISCs are located at the bases of colon crypts, maintain LGR5(+) intestinal stem cells, and protect against acute intestinal damage through enhanced R-spondin 1 production. The mechanism involves a reactive oxygen species (ROS)-MAP3K2-ERK5-KLF2 axis to augment WNT signaling for intestinal regeneration.
Article
Biochemistry & Molecular Biology
Renjun Tu, Bo Duan, Xiaoqing Song, Shiyuan Chen, Allison Scott, Kate Hall, Jillian Blanck, Dustin DeGraffenreid, Hua Li, Anoja Perera, Jeff Haug, Ting Xie
Summary: In the Drosophila ovary, inner germarial sheath (IGS) cells form multiple niche compartments that interact with different stages of germline stem cell (GSC) progeny, regulating stem cell self-renewal and stepwise differentiation.
Review
Developmental Biology
Jessica D. Manneken, Peter D. Currie
Summary: The cells of the innate immune system, particularly macrophages, play a vital role in tissue repair and stem cell activation. Recent studies have revealed the complex molecular processes involved in these mechanisms and the direct role of immune cells in coordinating these processes.
Article
Multidisciplinary Sciences
Guadalupe Gutierrez, Peng Sun, Yingying Han, Xing Dai
Summary: Breast cancer is a heterogeneous disease, and understanding the heterogeneity within the normal mammary basal epithelial cells is crucial. In this study, we used single-cell RNA-sequencing and RNA in-situ hybridization to characterize the heterogeneity of mammary basal cells. We identified four major transcriptional states within the basal cells, one of which represents a dynamic state during pubertal mammary morphogenesis.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Nathanael Andrews, Jason T. Serviss, Natalie Geyer, Agneta B. Andersson, Ewa Dzwonkowska, Iva Sutevski, Rosan Heijboer, Ninib Baryawno, Marco Gerling, Martin Enge
Summary: This study introduces a new method called CIM-seq, which can analyze cell-cell interactions in different tissues by sequencing cell multiplets. The results demonstrate that CIM-seq is applicable for unsupervised profiling of cell-type interactions in different tissue types.
Article
Biochemistry & Molecular Biology
Zhipeng Sun, Todd G. Nystul, Guohua Zhong
Summary: In this study, the researchers used single-cell RNA sequencing, RNAi screen, and bioinformatic analysis to identify genes involved in germ cell differentiation. They discovered that a gene named eggpl is highly expressed in ovarian germline stem cells and downregulated in early daughter cells. Knockdown of eggpl resulted in defects in germ cell proliferation and differentiation. Furthermore, the downregulation of eggpl was found to link nutritional status to germ cell proliferation and differentiation. This study provides new insights into the signaling networks regulating early germ cell development and identifies eggpl as an important player in this process.
Article
Medicine, Research & Experimental
Yan Lin, Quan Gu, Shihong Lu, Zengkai Pan, Zining Yang, Yapu Li, Shangda Yang, Yanling Lv, Zhaofeng Zheng, Guohuan Sun, Fanglin Gou, Chang Xu, Xiangnan Zhao, Fengjiao Wang, Chenchen Wang, Shiru Yuan, Xiaobao Xie, Yang Cao, Yue Liu, Weiying Gu, Tao Cheng, Hui Cheng, Xiaoxia Hu
Summary: Severe acute graft-versus-host disease (aGVHD) is a complication of allogeneic hematopoietic stem cell transplantation. The bone marrow (BM) niche is damaged in aGVHD hosts, and the use of the JAK1/2 inhibitor ruxolitinib can restore BM mesenchymal stromal cell (BMSC) function and improve hematopoietic dysfunction caused by aGVHD.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Simon Renders, Arthur Flohr Svendsen, Jasper Panten, Nicolas Rama, Maria Maryanovich, Pia Sommerkamp, Luisa Ladel, Anna Rita Redavid, Benjamin Gibert, Seka Lazare, Benjamin Ducarouge, Katharina Schoenberger, Andreas Narr, Manon Tourbez, Bertien Dethmers-Ausema, Erik Zwart, Agnes Hotz-Wagenblatt, Dachuan Zhang, Claudia Korn, Petra Zeisberger, Adriana Przybylla, Markus Sohn, Simon Mendez-Ferrer, Mathias Heikenwaelder, Maik Brune, Daniel Klimmeck, Leonid Bystrykh, Paul S. Frenette, Patrick Mehlen, Gerald de Haan, Nina Cabezas-Wallscheid, Andreas Trumpp
Summary: Haematopoietic stem cells are characterized by their self-renewal potential and dormancy. This study shows that niche-produced netrin-1 preserves HSC quiescence and self-renewal through neogenin-1, with a decline in netrin-1 production during aging leading to decreased Neo1-mediated HSC self-renewal.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Fangyuan Shen, Yu Shi
Summary: The article reviews the research progress of single-cell RNA sequencing technology in the identification of osteogenic markers and differentiation pathways, as well as the application of single-cell technology combined with experimental techniques in MSC-related new insights. It also summarizes the latest findings regarding the interaction between stem cell fate and the microenvironment in homeostasis and pathological processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biology
Susan Eliazer, Xuefeng Sun, Emilie Barruet, Andrew S. Brack
Summary: The muscle fiber maintains a continuum of quiescent states through a gradient of Notch ligand, Dll4, which is captured by QSCs. The abundance of Dll4 captured by the QSC correlates with the protein levels of the stem cell identity marker, Pax7. Niche-specific loss of Dll4 decreases QSC diversity and shifts the continuum to cell states that are biased toward more proliferative and committed fates.
Review
Medicine, Research & Experimental
Yangdan Liu, Chiakang Ho, Dongsheng Wen, Jiaming Sun, Lu Huang, Ya Gao, Qingfeng Li, Yifan Zhang
Summary: The self-renewal ability of stem cells is crucial for skin homeostasis, aging, and wound repair. COL17, as a component of the stem cell niche, plays a pivotal role in regulating stem cell maintenance and function.
Review
Cell Biology
Nancy C. C. Allen, Nabora S. S. Reyes, Jin Young Lee, Tien Peng
Summary: Aging is the final stage of development that leads to stereotyped changes in tissue morphology and increases the risk of chronic lung diseases. Inflammation and cellular senescence, two hallmarks of aging, are mutually related and contribute to increased susceptibility to chronic diseases in aging tissues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Masaru Mizukoshi, Masaru Kaku, Lay Thant, Kohei Kitami, Moe Arai, Isao Saito, Katsumi Uoshima
Summary: The study revealed that periodontal ligament (PDL) cells exhibit dynamic proliferative characteristics during orthodontic tooth movement-induced tissue remodeling, with proliferative cells likely undergoing further division and contributing to tissue remodeling. Additionally, proliferating cells expressed various molecular markers, indicating the lineage plasticity of PDL cells in vivo.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Andy Chan, Sophia Ma, Bret J. Pearson, Danny Chan
Summary: The extracellular matrix plays a crucial role in the maintenance, regeneration, and differentiation of stem cells within their niche. By studying the collagen gene family in the freshwater planarian Schmidtea mediterranea, researchers have identified a key gene, col4-1, which influences the proliferation of neoblasts. Furthermore, evidence suggests that an interaction between type IV collagen, the discoidin domain receptor, and neuregulin-7 regulate the balance of symmetric and asymmetric division of neoblasts through the NRG-7/EGFR pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Shin-Chih Lin, Chia-Hung Lin, Nien-Chu Shih, Hsin-Ling Liu, Wen-Chao Wang, Kun-Yang Lin, Zih-Yu Liu, Yu-Jhen Tseng, Hsueh-Kai Chang, Yi-Cheng Lin, Yi-Chen Yeh, Hiroshi Minato, Takeshi Fujii, Yu-Chung Wu, Mei-Yu Chen, Teh-Ying Chou
Article
Developmental Biology
Elham Rastegari, Kreeti Kajal, Boon-Shing Tan, Fu Huang, Ruey-Hwa Chen, Tao-Shieh Hsieh, Hwei-Jan Hsu
Review
Biology
Kun-Yang Lin, Hwei-Jan Hsu
CURRENT OPINION IN INSECT SCIENCE
(2020)
Article
Cell Biology
Oyundari Amartuvshin, Chi-Hung Lin, Shao-Chun Hsu, Shih-Han Kao, Alvin Chen, Wei-Chun Tang, Han-Lin Chou, Dong-Lin Chang, Yen-Yang Hsu, Bai-Shiou Hsiao, Elham Rastegari, Kun-Yang Lin, Yu-Ting Wang, Chi-Kuang Yao, Guang-Chao Chen, Bi-Chang Chen, Hwei-Jan Hsu
Article
Multidisciplinary Sciences
Kun-Yang Lin, Wen-Der Wang, Chi-Hung Lin, Elham Rastegari, Yu-Han Su, Yu-Tzu Chang, Yung-Feng Liao, Yi-Chieh Chang, Haiwei Pi, Bo-Yi Yu, Shu-Hwa Chen, Chung-Yen Lin, Mei-Yeh Lu, Tsu-Yi Su, Fei-Yang Tzou, Chih-Chiang Chan, Hwei-Jan Hsu
NATURE COMMUNICATIONS
(2020)
Article
Developmental Biology
Tzu-Lun Tseng, Ying-Ting Wang, Chang-Yu Tsao, Yi-Teng Ke, Yi-Ching Lee, Hwei-Jan Hsu, Kenneth D. Poss, Chen-Hui Chen
Summary: Research identified a gene called pan in zebrafish that can suspend juvenile growth but resume a robust growth trajectory after release from restriction, with the mechanism being conserved in flies, zebrafish, and mice. This suggests that safe, reversible pauses of juvenile growth can be mediated by targeting the activity of a single gene.
Review
Plant Sciences
Chao-Yuan Yu, Yueh Cho, Oshin Sharma, Kazue Kanehara
Summary: This review discusses the uniqueness of plants in the conserved unfolded protein response mechanism. While plants and animals share similarities in the molecular mechanisms of UPR, recent studies have revealed the distinctiveness of plant UPR, with plant-specific protein families playing crucial roles.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Kazue Kanehara, Yueh Cho, Chao-Yuan Yu
Summary: This review discusses the plant unfolded protein response and compares it with the respective systems in other model organisms from the perspective of membrane phospholipids.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Cell Biology
Ting-An Chen, Kun-Yang Lin, Shun-Min Yang, Chen-Yuan Tseng, Yu-Ting Wang, Chi-Hung Lin, Lichao Luo, Yu Cai, Hwei-Jan Hsu
Summary: This study reveals the crucial role of canonical Wnt signaling in ovarian development and highlights the function of a physical permeability barrier in regulating morphogen distribution.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chi-Hung Lin, Tzu-Yang Lin, Shao-Chun Hsu, Hwei-Jan Hsu
Summary: Mitochondrial morphology can modulate organelle function and affect stem cell behavior and tissue homeostasis. Accumulation of fragmented mitochondria leads to age-dependent loss of germline stem cells in aged Drosophila ovaries. Current methods for examining mitochondrial morphology have limitations in resolution and sampling. We present a simple and inexpensive nanoscale optical imaging method using expansion microscopy.
Article
Cell Biology
Chieh-Ting Fang, Hsiao-Hui Kuo, Oyundari Amartuvshin, Hwei-Jan Hsu, Sih-Long Liu, Jhong-Syuan Yao, Ling-Huei Yih
Summary: The regulation of AMPK and ACC in the fatty acid synthesis pathway is crucial for tubulin palmitoylation and precise control of spindle assembly.
CELL DEATH DISCOVERY
(2023)
Article
Cell Biology
Mahekta R. Gujar, Yang Gao, Xiang Teng, Qiannan Deng, Kun-Yang Lin, Ye Sing Tan, Yusuke Toyama, Hongyan Yang
Summary: The ability of stem cells to switch between quiescent and proliferative states is crucial for tissue homeostasis and regeneration. This study establishes Drosophila qNSCs as a regeneration model and identifies the Arf1/Sec71-Msps pathway in the regulation of microtubule growth and NSC reactivation.
DEVELOPMENTAL CELL
(2023)