Review
Cell & Tissue Engineering
Jing Kong, Shiyuan Wen, Wenjing Cao, Peng Yue, Xin Xu, Yu Zhang, Lisha Luo, Taigui Chen, Lianbao Li, Feng Wang, Jian Tao, Guozhong Zhou, Suyi Luo, Aihua Liu, Fukai Bao
Summary: Organoids derived from stem cells or organ-specific progenitors display structures and functions consistent with organs in vivo. Lung organoids are widely used in modeling injury to and repair of lungs, as well as understanding the mechanisms of epithelial repair.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Developmental Biology
Lauren A. Shechtman, Jennifer K. Scott, Eric D. Larson, Trevor J. Isner, Bryan J. Johnson, Dany Gaillard, Peter J. Dempsey, Linda A. Barlow
Summary: The taste buds on the tongue consist of taste receptor cells that detect different tastes. These cells are derived from basal keratinocytes that express the transcription factor SOX2. The level of SOX2 expression determines the progenitor potential, where higher levels give rise to both taste and non-taste lingual epithelium, and lower levels give rise to only non-taste cells. The signaling pathway of WNT/beta-catenin promotes taste cell differentiation in organoids derived from high SOX2+ expressing progenitors.
Article
Multidisciplinary Sciences
Ansley S. Conchola, Tristan Frum, Zhiwei Xiao, Peggy P. Hsu, Kamika Kaur, Michael S. Downey, Renee F. C. Hein, Alyssa J. Miller, Yu- Hwai Tsai, Angeline Wu, Emily M. Holloway, Abhinav Anand, Preetish Kadur Lakshminarasimha Murthy, Ian Glass, Purushothama R. Tata, Jason R. Spence
Summary: Using scRNA-seq and microscopy, a cell enriched in the lower airways of developing human lung was identified by the coexpression of SCGB3A2/SFTPB/CFTR. By applying the CellTagging single-cell barcode-based lineage tracing method, the fate of SCGB3A2/SFTPB/CFTR cells during airway organoid differentiation was tracked, revealing their distinct differentiation potential and contribution to epithelial diversity in this region. These findings were supported by studies using organoids and isolated cells from the lower noncartilaginous airway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Jingsi Yang, Nan Ding, Dandan Zhao, Yunsheng Yu, Chunlai Shao, Xuan Ni, Zhen-Ao Zhao, Zhen Li, Jianquan Chen, Zheng Ying, Miao Yu, Wei Lei, Shijun Hu
Summary: The study developed a new strategy of using EBSS treatment to mimic in vivo starvation event, promoting the maturation of hESC-CMs. Intermittent starvation through EBSS significantly enhanced structural, metabolic, and electrophysiological maturity of hESC-CMs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Shuyu Liu, Dawei Sun, Richard Butler, Emma L. Rawlins
Summary: This study expands multipotent epithelial progenitor cells from human embryonic lungs as organoids and maintains their self-renewing state. The cells exhibit columnar shape, resembling the developing lung tip epithelium. The study identifies the signaling pathways, including FGF7 and integrin signaling, that regulate cell shape and development in human lung progenitors.
Article
Chemistry, Multidisciplinary
Intan Rosalina Suhito, Jin Won Kim, Kyeong-Mo Koo, Sun Ah Nam, Yong Kyun Kim, Tae-Hyung Kim
Summary: A new method is proposed for the real-time assessment of kidney organoids, allowing non-destructive evaluation of differentiation and maturation levels. This method utilizes electrochemical signals to differentiate kidney cells from off-target cells, providing a promising tool for organoid research.
Article
Developmental Biology
Renee F. C. Hein, Ansley S. Conchola, Alexis S. Fine, Zhiwei Xiao, Tristan Frum, Lindy K. Brastrom, Mayowa A. Akinwale, Charlie J. Childs, Yu-Hwai Tsai, Emily M. Holloway, Sha Huang, John Mahoney, Idse Heemskerk, Jason R. Spence
Summary: This study successfully developed an iPSC organoid model enriched with NKX2-1(+) BTP-like cells. By optimizing the differentiation process and using single cell RNA sequencing analysis, researchers demonstrated a high degree of transcriptional similarity between the induced BTPs (iBTPs) and in vivo BTPs. This model is important for studying human lung development and differentiation.
Review
Toxicology
Atsushi Masui, Toyohiro Hirai, Shimpei Gotoh
Summary: The absence of in vitro platforms for human pulmonary toxicology studies is a growing concern. Recent advances in culture methods, specifically human pluripotent stem cell-derived lung epithelial organoid culture systems, have provided potential solutions. These advancements have allowed for the in vitro recapitulation of genetic lung diseases and the evaluation of the effectiveness and toxicity of therapeutic agents, bridging the gap between bench and bedside.
ARCHIVES OF TOXICOLOGY
(2022)
Article
Oncology
Akihiro Miura, Daisuke Yamada, Masahiro Nakamura, Shuta Tomida, Dai Shimizu, Yan Jiang, Tomoka Takao, Hiromasa Yamamoto, Ken Suzawa, Kazuhiko Shien, Masaomi Yamane, Masakiyo Sakaguchi, Shinichi Toyooka, Takeshi Takarada
Summary: Utilizing human induced pluripotent stem cells (hiPSCs), researchers established iHER2-hiPSCs and differentiated lung progenitors to form human lung organoids (HLOs), demonstrating that overexpression of HER2 leads to increased morphological irregularity and proliferation capacity in lung tissue, resembling precancerous lesions. HiPSC-derived HLOs serve as a model to study the early tumorigenesis of lung adenocarcinoma and provide insights into the molecular basis of tumor initiation and progression.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Biochemistry & Molecular Biology
Jong-Tae Kim, Tae Yeon Kim, Dong Hyuk Youn, Sung Woo Han, Chan Hum Park, Younghyurk Lee, Harry Jung, Jong Kook Rhim, Jeong Jin Park, Jun Hyong Ahn, Heung Cheol Kim, Sung Min Cho, Jin Pyeong Jeon
Summary: This study evaluated the therapeutic efficacy of human embryonic stem cell-derived cerebral organoids in a mild traumatic brain injury (TBI) model. The results showed that transplantation of the organoids reduced neuronal cell death, promoted neurogenesis, and improved cognitive function. Furthermore, angiogenesis was observed in the engrafted organoids, and microarray analysis revealed neuronal differentiation potential and higher expression of proteins associated with neurogenesis, angiogenesis, and extracellular matrix remodeling.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Hiroo Sasaki, Hidetaka Suga, Kazuhito Takeuchi, Yuichi Nagata, Hideyuki Harada, Tatsuma Kondo, Eiji Ito, Sachi Maeda, Mayu Sakakibara, Mika Soen, Tsutomu Miwata, Tomoyoshi Asano, Hajime Ozaki, Shiori Taga, Atsushi Kuwahara, Tokushige Nakano, Hiroshi Arima, Ryuta Saito
Summary: We have successfully developed pituitary organoids (POs) derived from human embryonic stem cells (hESC). Transplantation of these POs into hypopituitary severe combined immunodeficient (SCID) mice was able to effectively restore ACTH secretion, thus providing a potential treatment for hypopituitarism.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Constantine A. Simintiras, Pramod Dhakal, Chaman Ranjit, Harriet C. Fitzgerald, Ahmed Z. Balboula, Thomas E. Spencer
Summary: The study developed a high-throughput method for isolating intraorganoid fluid (IOF) from human endometrial epithelial organoids, revealing distinct biochemical differences between IOF and extraorganoid fluid (EOF). This has the potential to enhance our understanding of uterine fluid composition and regulation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Takeshi Ouchi, Sohshi Morimura, Lukas E. Dow, Hiroyuki Miyoshi, Mark C. Udey
Summary: The study reveals the functional role of EpCAM in intestinal epithelial cells and its relation to intestinal disorders. Deletion of EpCAM leads to increased apoptosis, decreased cell proliferation, and disruptions in tight junctions and cell polarity in organoids and spheroids. Treatment with Rho-associated coiled-coil kinase (ROCK) inhibitors improves epithelial integrity in EpCAM-deficient cells.
Article
Medicine, Research & Experimental
Soraya Savoj, Mohammad Hossein Nasr Esfahani, Akbar Karimi, Fereshteh Karamali
Summary: This study assessed the inducing activity of SCAP in the differentiation of hESCs into retinal cells in a 3D organoid system. Increasing the number of SCAPs improved the formation of the optic vesicle structure.
Article
Gastroenterology & Hepatology
Hana Lee, Kwang Bo Jung, Ohman Kwon, Ye Seul Son, Eunho Choi, Won Dong Yu, Naeun Son, Jun Hyoung Jeon, Hana Jo, Haneol Yang, Yeong Rak Son, Chan-Seok Yun, Hyun-Soo Cho, Sang Kyu Kim, Dae-Soo Kim, Doo-Sang Park, Mi-Young Son
Summary: This study investigates the modulatory capacity of a newly isolated probiotic strain (DS0384) on early intestinal development. They found that DS0384 accelerated the maturation of fetal intestines and promoted intestinal stem cell proliferation by secreting a specific metabolite (NCG). The results suggest that DS0384 has potential probiotic properties and therapeutic applications for early gut development disorders.
Meeting Abstract
Biochemistry & Molecular Biology
Ignacio Prieto, Alberto Zambrano, Javier Laso, Ana Aranda, Enrique Samper, Maria Monsalve
FREE RADICAL BIOLOGY AND MEDICINE
(2018)
Article
Neurosciences
A. Bernabeu-Zornoza, R. Coronel, C. Palmer, M. Calero, A. Martinez-Serrano, E. Cano, Alberto Zambrano, Isabel Liste
MOLECULAR NEUROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Trinidad Guijarro, Esmeralda Magro-Lopez, Joana Manso, Ricardo Garcia-Martinez, Maria Jesus Fernandez-Acenero, Isabel Liste, Alberto Zambrano
MOLECULAR MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Ignacio Prieto, Alberto Zambrano, Javier Laso, Ana Aranda, Enrique Samper, Maria Monsalve
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Environmental Sciences
Laura Sandoval, Andreea Rosca, Andreea Oniga, Alberto Zambrano, Juan Jose Ramos, Ma Carmen Gonzalez, Isabel Liste, Miguel Motas
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Cell & Tissue Engineering
Helena Meyer-Berg, Lucia Zhou Yang, Maria Pilar de Lucas, Alberto Zambrano, Stephen C. Hyde, Deborah R. Gill
STEM CELL RESEARCH & THERAPY
(2020)
Review
Biochemistry & Molecular Biology
Esmeralda Magro-Lopez, Maria Angeles Munoz-Fernandez
Summary: BMPs are multifunctional growth factors that play essential roles in regulating the development and function of the female reproductive system. They are involved in reproductive system development and the differentiation of human pluripotent stem cells into reproductive system tissues or organoids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Esmeralda Magro-Lopez, Irene Chamorro-Herrero, Alberto Zambrano
Summary: Contrary to expectations, the biologically active form of vitamin D did not have therapeutic effects in bleomycin-induced lung fibrosis but instead aggravated lung pathology. The current study focused on evaluating the effects of two hypocalcemic vitamin D analogs on DNA damage expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Julio Sempere, Suelen Andreia Rossi, Irene Chamorro-Herrero, Fernando Gonzalez-Camacho, Maria Pilar de Lucas, Jose Maria Rojas-Cabaneros, Carlos Pelleschi Taborda, Oscar Zaragoza, Jose Yuste, Alberto Zambrano
Summary: The use of human pluripotent stem cells to generate lung bud organoids provides a promising strategy for studying the interaction between Streptococcus pneumoniae and the human lung. These organoids can model important events of pneumococcal infection, such as bacterial adherence, internalization, and the innate immune response. This model offers a reliable human context to investigate pneumococcal virulence factors and develop therapeutic interventions.
MICROBIOLOGY SPECTRUM
(2022)
Review
Biochemistry & Molecular Biology
Irene Chamorro-Herrero, Alberto Zambrano
Summary: Respiratory diseases are a major cause of morbidity and mortality globally. Current treatments are mainly symptomatic, so there is a need for new strategies to understand these diseases and develop effective therapies. Stem cell and organoid technology has allowed the development of human pluripotent stem cell lines and differentiation protocols to create airways and lung organoids for disease modeling. Specifically, these organoids have been used to model respiratory diseases such as idiopathic pulmonary fibrosis, cystic fibrosis, chronic obstructive pulmonary disease, and COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Biotechnology & Applied Microbiology
Helena C. M. Meyer-Berg, Lucia Zhou Yang, Maria Pilar De Lucas, Alberto Zambrano, Stephen C. Hyde, Deborah R. Gill
Review
Cell Biology
Adela Bernabeu-Zornoza, Raquel Coronel, Charlotte Palmer, Maria Monteagudo, Alberto Zambrano, Isabel Liste
NEURAL REGENERATION RESEARCH
(2019)
Review
Cell Biology
Raquel Coronel, Charlotte Palmer, Adela Bernabeu-Zornoza, Maria Monteagudo, Andreea Rosca, Alberto Zambrano, Isabel Liste
NEURAL REGENERATION RESEARCH
(2019)