Article
Biochemistry & Molecular Biology
Petra Malavasic, Sara Polajzer, Nika Lovsin
Summary: Genome-wide association studies have identified a genetic locus, ANAPC1, that is significantly associated with femoral neck bone mineral density and plays a role in bone physiology and the development of osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Li Quan, Xinxin Sun, Linghui Xu, Rui Ai Chen, Ding Xiang Liu
Summary: Disruption of the cell cycle is a common strategy used by many viruses to create a favorable cellular microenvironment for their replication. In this study, we demonstrate that the gamma-coronavirus infectious bronchitis virus (IBV) nonstructural protein 12 (nsp12) interacts with the p50 regulatory subunit of DNA polymerase delta (pol delta), as well as with nsp13, to induce cell cycle arrest in the S phase. Interestingly, nsp12 from severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 also interacted with p50. These findings highlight the importance of these interactions in coronavirus replication and virus-host interaction, and suggest potential targets for antiviral intervention.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Plant Sciences
Jan Martinek, Petra Cifrova, Stanislav Vosolsobe, Judith Garcia-Gonzalez, Katerina Malinska, Zdenka Mauerova, Barbora Jelinkova, Jana Krtkova, Lenka Sikorova, Ian Leaves, Imogen Sparkes, Katerina Schwarzerova
Summary: This study demonstrates that ARP2/3 is involved in the process of peroxisome degradation by autophagy, called pexophagy, in plants through its interaction with the autophagy-related protein subunit ATG8f.
Article
Cell Biology
Katharina Goertemaker, Chad Yee, Rabea Bartoelke, Heide Behrmann, Jan-Oliver Voss, Jessica Schmidt, Jingjing Xu, Vita Solovyeva, Bo Leberecht, Elmar Behrmann, Henrik Mouritsen, Karl-Wilhelm Koch
Summary: This study reveals the interaction between the alpha-subunit of the cone-specific heterotrimeric G protein and the photolyase homolog in European robin, and confirms its existence in living cells. These findings support the important role of light-induced radical-pair formation in the bird's retina, which may be the first biochemical signaling step in magnetoreception.
Review
Biochemistry & Molecular Biology
Jorrit M. Enserink, Pierre Chymkowitch
Summary: Cdk1 is a master regulator of the cell cycle, controlling progression through different phases. It also directly regulates RNA polymerase activity, which is important for protein synthesis and meeting cellular demands. This regulation promotes cell cycle entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Joseph M. Kelich, Harry Papaioannou, Emmanuel Skordalakes
Summary: The human CST complex plays a critical role in telomere maintenance and homeostasis, with its dynamic intracellular localization being cell cycle-dependent. The CST complex lacks a nuclear localization signal and relies on Pol alpha-primase for nuclear import. Mutations affecting CST nuclear import are associated with telomere syndromes and cancer, underscoring the importance of this process in health.
COMMUNICATIONS BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Haein Weon, Jiyeon Jun, Tae Wan Kim, Kibeom Park, Hyung Kyu Kim, Dong-ho Youn
Summary: In this study, it was found that interrupting the association between alpha and beta subunits in VDCC complex can inhibit channel activation, leading to reduced excitatory neurotransmission and arterial blood pressure.
Article
Multidisciplinary Sciences
Krishnendu Khan, Briana Long, Valentin Gogonea, Gauravi M. Deshpande, Kommireddy Vasu, Paul L. Fox
Summary: Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in the translation process that ligate amino acids to their corresponding transfer RNAs (tRNAs). In mammalian cells, these enzymes, along with AIMPs proteins, form a large multi-tRNA synthetase complex (MSC), whose assembly mechanism and function are still unclear. This study reveals the importance of cotranslational interactions, particularly involving AIMPs proteins, in the assembly process of the MSC. Interestingly, these cotranslational interactions sometimes involve more than two proteins, suggesting a diverse pathway for the ordered assembly of small subcomplexes into larger complexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Christoph Diehl, Patrick D. Gerlinger, Nicole Paczia, Tobias J. Erb
Summary: Anaplerosis, a feature of metabolism, has been applied to synthetic in vitro metabolic networks to achieve carbon-positive synthesis of compounds. In this research, anaplerotic strategies were used to access a new-to-nature CO2-fixation pathway and successfully produce 6-deoxyerythronolide B. This work demonstrates the design and customization of anaplerotic modules to enhance and expand synthetic capabilities in complex in vitro reaction networks.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ziyang Yao, Jianhua Wu, Ying Fang
Summary: The binding of CD44 to HA plays a crucial role in cell adhesion, motility, metastasis, inflammatory responses, and tumor development. This study investigated the interaction between CD44 HABD and HA under diverse mechanical constraints. The findings provide insights into the regulation mechanism and molecular basis of CD44-HA interaction and its relevance to cell inflammatory responses and tumor development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jin-Tae Choi, Yeseul Choi, Yujin Lee, Seung-Heon Lee, Seun Kang, Kyung-Tae Lee, Yong-Sun Bahn
Summary: This study identifies the crucial role of the fungal RAVE complex in the pathogenicity of Cryptococcus neoformans and suggests a novel therapeutic strategy for controlling cryptococcosis.
Article
Multidisciplinary Sciences
Tomoaki Kawakami, Long-Jiang Yu, Tai Liang, Koudai Okazaki, Michael T. Madigan, Yukihiro Kimura, Zheng-Yu Wang-Otomo
Summary: The study achieved co-crystallization of a bacterial photosynthetic electron transfer complex, offering insights into the molecular basis of interprotein electron tunneling and providing a detailed model for long-range trans-protein electron tunneling pathways in biological systems. The high potential iron-sulfur proteins play a crucial role as direct electron donors in LH1-RC complexes in photosynthetic beta- and gamma-Proteobacteria, with implications for the electron transfer pathway discussed based on the crystal structure of the HiPIP-bound LH1-RC complex.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Cyril Djari, Isabelle Sahut-Barnola, Amandine Septier, Ingrid Plotton, Nathanaelle Montanier, Damien Dufour, Adrien Levasseur, James Wilmouth, Jean-Christophe Pointud, Fabio R. Faucz, Crystal Kamilaris, Antoine-Guy Lopez, Florian Guillou, Amanda Swain, Seppo J. Vainio, Igor Tauveron, Pierre Val, Herve Lefebvre, Constantine A. Stratakis, Antoine Martinez, Anne-Marie Lefrancois-Martinez
Summary: The occurrence of Large-cell Calcifying Sertoli Cell Tumors requires mutations in both stromal and Sertoli cells. Integrative analyses led to the understanding of human LCCSCT pathogenesis and demonstrated PKA-induced paracrine molecular circuits.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell Biology
Dilyana Georgieva Kirova, Kristyna Judasova, Julia Vorhauser, Thomas Zerjatke, Jacky Kieran Leung, Ingmar Glauche, Joerg Mansfeld
Summary: Reactive oxygen species (ROS) play a role in regulating cell proliferation and DNA replication through modulating the activity of cyclin-dependent kinase 2 (CDK2). The increase in mitochondrial ROS levels during the cell cycle is shown to be important for regulating CDK2 activity through oxidation of a cysteine residue on CDK2.
DEVELOPMENTAL CELL
(2022)
Article
Biochemistry & Molecular Biology
Sven Vanselow, Lea Neumann-Arnold, Franziska Wojciech-Moock, Wolfgang Seufert
Summary: This study reveals the assembly mechanism and individual functions of different subunits of the eukaryotic translation initiation factor 2 (eIF2). The research shows that a conformational change in the eIF2 gamma subunit, mediated by Cdc123, exposes binding sites for eIF2 alpha and eIF2 beta subunits. Contrary to previous hypotheses, the associations between eIF2 alpha and eIF2 beta with the gamma subunit are independent of each other. Furthermore, the resulting heterodimers are nonfunctional and fail to bind the guanosine exchange factor eIF2B. This study provides insights into an essential step in translation initiation, with implications for understanding human diseases related to dysfunction in this process.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)