The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis
Published 2018 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis
Authors
Keywords
-
Journal
Scientific Reports
Volume 8, Issue 1, Pages -
Publisher
Springer Nature
Online
2018-04-13
DOI
10.1038/s41598-018-24573-y
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Comparative analysis of vaginal microbiota sampling using 16S rRNA gene analysis
- (2017) Seppo Virtanen et al. PLoS One
- Normalization and microbial differential abundance strategies depend upon data characteristics
- (2017) Sophie Weiss et al. Microbiome
- Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal Communities
- (2017) Anna Vesty et al. PLoS One
- Revised Estimates for the Number of Human and Bacteria Cells in the Body
- (2016) Ron Sender et al. PLOS BIOLOGY
- Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics
- (2016) Juan Jovel et al. Frontiers in Microbiology
- An assessment of US microbiome research
- (2016) Elizabeth Stulberg et al. Nature Microbiology
- Body Site Is a More Determinant Factor than Human Population Diversity in the Healthy Skin Microbiome
- (2016) Guillermo I. Perez Perez et al. PLoS One
- Evaluation of Lysis Methods for the Extraction of Bacterial DNA for Analysis of the Vaginal Microbiota
- (2016) Christina Gill et al. PLoS One
- The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies
- (2015) J Paul Brooks et al. BMC MICROBIOLOGY
- Effect of preservation method on spider monkey (Ateles geoffroyi) fecal microbiota over 8weeks
- (2015) Vanessa L. Hale et al. JOURNAL OF MICROBIOLOGICAL METHODS
- Sample storage conditions significantly influence faecal microbiome profiles
- (2015) Jocelyn M Choo et al. Scientific Reports
- Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differences
- (2015) Brett Wagner Mackenzie et al. Frontiers in Microbiology
- Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
- (2014) Susannah J Salter et al. BMC BIOLOGY
- Comparison of methods for fecal microbiome biospecimen collection
- (2014) Christine Dominianni et al. BMC MICROBIOLOGY
- Primate vaginal microbiomes exhibit species specificity without universal Lactobacillus dominance
- (2014) Suleyman Yildirim et al. ISME Journal
- Cultivated Vaginal Microbiomes Alter HIV-1 Infection and Antiretroviral Efficacy in Colonized Epithelial Multilayer Cultures
- (2014) Richard B. Pyles et al. PLoS One
- The Impact of Different DNA Extraction Kits and Laboratories upon the Assessment of Human Gut Microbiota Composition by 16S rRNA Gene Sequencing
- (2014) Nicholas A. Kennedy et al. PLoS One
- Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing
- (2014) Loreto Abusleme et al. Journal of Oral Microbiology
- Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform
- (2013) James J. Kozich et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- The Variability of the 16S rRNA Gene in Bacterial Genomes and Its Consequences for Bacterial Community Analyses
- (2013) Tomáš Větrovský et al. PLoS One
- Complement modulates the cutaneous microbiome and inflammatory milieu
- (2013) C. Chehoud et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Selection of primers for optimal taxonomic classification of environmental 16S rRNA gene sequences
- (2012) David A W Soergel et al. ISME Journal
- Effects of PCR cycle number and DNA polymerase type on the 16S rRNA gene pyrosequencing analysis of bacterial communities
- (2012) Jae-Hyung Ahn et al. JOURNAL OF MICROBIOLOGY
- The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
- (2012) Christian Quast et al. NUCLEIC ACIDS RESEARCH
- Evaluation of Methods for the Extraction and Purification of DNA from the Human Microbiome
- (2012) Sanqing Yuan et al. PLoS One
- Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples
- (2012) Dana Willner et al. PLoS One
- Comparison of Storage Conditions for Human Vaginal Microbiome Studies
- (2012) Guoyun Bai et al. PLoS One
- UCHIME improves sensitivity and speed of chimera detection
- (2011) Robert C. Edgar et al. BIOINFORMATICS
- Effect of DNA Extraction Method on the Apparent Microbial Diversity of Soil
- (2010) O. Inceoglu et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method
- (2010) Jin-Ya Wu et al. BMC MICROBIOLOGY
- Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
- (2009) P. D. Schloss et al. APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Microbial community profiling for human microbiome projects: Tools, techniques, and challenges
- (2009) M. Hamady et al. GENOME RESEARCH
- Statistical Methods for Detecting Differentially Abundant Features in Clinical Metagenomic Samples
- (2009) James Robert White et al. PLoS Computational Biology
Find Funding. Review Successful Grants.
Explore over 25,000 new funding opportunities and over 6,000,000 successful grants.
ExploreAsk a Question. Answer a Question.
Quickly pose questions to the entire community. Debate answers and get clarity on the most important issues facing researchers.
Get Started