Article
Engineering, Biomedical
Lauren A. Boller, Stefanie M. Shiels, David C. Florian, Sun H. Peck, Jonathan G. Schoenecker, Craig Duvall, Joseph C. Wenke, Scott A. Guelcher
Summary: This study explores the effects of nHA dispersed in polymeric scaffolds on bone regeneration, finding that nHA has a dose-dependent impact on osteogenesis and promotes new bone formation in a site-specific manner. The findings provide new insights into designing synthetic bone grafts for complex fracture cases.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Angela Fernandez-Iglesias, Rocio Fuente, Helena Gil-Pena, Laura Alonso-Duran, Fernando Santos, Jose Manuel Lopez
Summary: The study investigated the formation of the epiphyseal bone plate using various microscopy techniques and found that the bone tissue is generated through a process different from the usual endochondral ossification. Osteoblasts deposit bone matrix on various surfaces, leading to the establishment of a core of bone sheet and subsequent thickening and reshaping. The processes occur in close relationship with cartilage, providing insight into the complex cellular events underlying bone formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rotem Kalev-Altman, Jerome Nicolas Janssen, Nir Ben-Haim, Tamar Levy, Astar Shitrit-Tovli, Joshua Milgram, Ron Shahar, Dalit Sela-Donenfeld, Efrat Monsonego-Ornan
Summary: The gelatinases MMP2 and MMP9 play distinct roles in skeletal development, with MMP2 involved in intramembranous ossification and MMP9 affecting endochondral ossification. Double knockout mice lacking both gelatinases show phenotypes resembling the single knockout mice, indicating the separate control of different bones by each gelatinase.
Article
Cell Biology
Jonathan C. Bernhard, Darja Marolt Presen, Ming Li, Xavier Monforte, James Ferguson, Gabriele Leinfellner, Patrick Heimel, Susanna L. Betti, Sharon Shu, Andreas H. Teuschl-Woller, Stefan Tangl, Heinz Redl, Gordana Vunjak-Novakovic
Summary: In this study, the effects of endochondral and intramembranous ossification pathways on the properties of engineered bone grafts were evaluated. The results showed that grafts generated through endochondral pathway exhibited better bone regeneration and vascularization, making it more suitable for regenerating large defects. On the other hand, the intramembranous ossification pathway could be used to guide tissue formation with scaffold architecture.
Review
Dentistry, Oral Surgery & Medicine
R. S. Marcucio, T. Miclau, C. S. Bahney
Summary: This review discusses the relationship between mechanisms of bone formation and bone regeneration, highlighting that regeneration is not simply a reinduction of the molecular and cellular programs used for development. The mechanical environment plays a significant role in repair, while cell-intrinsic processes drive skeletal formation.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Marzia Ferretti, Carla Palumbo
Summary: Static (SO) and dynamic (DO) osteogenesis involve differing characteristics in terms of osteoblast arrangement, motion, time of occurrence, sensitivity to conditioning factors, distribution of resulting osteocytes, collagen texture, mechanical properties of the formed bone, and overall functions.
APPLIED SCIENCES-BASEL
(2021)
Article
Orthopedics
Joseph A. Panos, Michael J. Coenen, Christopher V. Nagelli, Erin B. McGlinch, Aysegul Atasoy-Zeybek, Consuelo Lopez De Padilla, Rodolfo E. De la Vega, Christopher H. Evans
Summary: This study compared the bone regeneration in a rat femoral defect model under native, impaired, and growth factor-stimulated conditions. Native bone regeneration resulted in a fully healed defect, while impaired bone regeneration led to fibrofatty consolidation resembling an atrophic non-union. Treatment with rhBMP2 promoted rapid bone formation but resulted in inferior mechanical properties compared to natural healing. Technologies favoring endochondral regenerative ossification would improve clinical outcomes.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Pathology
Kristin Olstad, Stina Ekman, Sigriour Bjornsdottir, Cathrine T. Fjordbakk, Kerstin Hansson, Sigurdur F. Sigurdsson, Charles J. Ley
Summary: Recently, a study examined the central and third tarsal bones of equine fetuses and foals using micro-computed tomography. Radiological changes, including incomplete ossification and focal ossification defects interpreted as osteochondrosis, were found in 16 out of 23 cases. Histological confirmation is needed to determine if vascular failure is the cause of these defects. This study aimed to further examine these bones and describe the tissues present, cartilage canals, and suspected osteochondrosis lesions.
VETERINARY PATHOLOGY
(2023)
Article
Developmental Biology
Naoki Kubo, Tetsuya Awada, Naoto Hirose, Makoto Yanoshita, Mami Takano, Sayuri Nishiyama, Eri Tsuboi, Daiki Kita, Shota Ito, Ayaka Nakatani, Azusa Onishi, Yuki Asakawa, Kotaro Tanimoto
Summary: Estrogen suppresses mandibular growth in a dose-dependent manner, with high doses leading to a significant increase in growth hormone and influencing endochondral ossification.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Materials Science, Biomaterials
Emilio Satoshi Hara, Noriyuki Nagaoka, Masahiro Okada, Takayoshi Nakano, Takuya Matsumoto
Summary: This study reveals the distinct microstructures of bone apatite clusters in intramembranous ossification (IO) and endochondral ossification (EO), and how these differences impact the growth of apatite clusters. The findings offer insightful inspirations for the design of more efficient materials for bone tissue engineering and repair.
Article
Anatomy & Morphology
Bruce Rothschild
Summary: The identification of skeletal maturity is important for understanding species longevity and maximal achievable size. This study examines the subchondral transcortical channels and articular vascular channels in long bones of birds to determine the point at which skeletal growth cessation occurs.
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Yuki Ikeda, Shoichiro Tani, Takeshi Moriishi, Aiko Kuroda, Yuki Matsuo, Naoya Saeki, Chizuko Inui-Yamamoto, Makoto Abe, Takashi Maeda, David W. Rowe, Ung-il Chung, Hironori Hojo, Yuki Matsushita, Takashi Sawase, Shinsuke Ohba
Summary: This study successfully developed a pluripotent stem cell system that models the intramembranous ossification process in humans and induces two distinct modes of ossification by controlling cell fate.
REGENERATIVE THERAPY
(2023)
Review
Endocrinology & Metabolism
Mari Satoh, Yukihiro Hasegawa
Summary: Bone age is an important marker of bone maturation, influenced by various hormones including estrogens. Females generally have more advanced bone maturation than males. This study reviews the factors affecting bone age progression, as well as bone maturation and deformities in certain medical conditions.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Orthopedics
Marjolein M. J. Caron, Bert van Rietbergen, Tessy M. R. Castermans, Mirella J. J. Haartmans, Lodewijk W. van Rhijn, Tim J. M. Welting, Adhiambo M. A. Witlox
Summary: The study suggests that naproxen and MTX have an impact on endochondral ossification, leading to impaired skeletal development.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Changshui Wang, Changmeng Cui, Pengfei Xu, Li Zhu, Hongjia Xue, Beibei Chen, Pei Jiang
Summary: Depression is often accompanied by disrupted energy metabolism, specifically impairments in the tricarboxylic acid (TCA) cycle and mitochondrial oxidative phosphorylation. This study revealed that chronic social defeat stress (CSDS) exposure inhibited the TCA cycle and decreased mitochondrial oxidative phosphorylation. The gatekeeper of the TCA cycle, pyruvate dehydrogenase (PDH), was suppressed, leading to impaired energy metabolism. The study also found that elevated levels of glucocorticoids (GCs) directly regulated PDH through the glucocorticoid receptor (GR), contributing to the manifestation of depression.
MOLECULAR PSYCHIATRY
(2023)
Article
Cell Biology
Bo Gao, Xisheng Lin, Huan Jing, Jing Fan, Chenchen Ji, Qiang Jie, Chao Zheng, Di Wang, Xiaolong Xu, Yaqian Hu, Weiguang Lu, Zhuojing Luo, Liu Yang
Article
Medicine, General & Internal
Chao Zheng, Xisheng Lin, Xiaolong Xu, Cheng Wang, Jinru Zhou, Bo Gao, Jing Fan, Weiguang Lu, Yagian Hu, Qiang Jie, Zhuojing Luo, Liu Yang
Article
Biochemistry & Molecular Biology
Chao Zheng, Xisheng Lin, He Liu, Weiguang Lu, Xiaolong Xu, Di Wang, Bo Gao, Cheng Wang, Jinru Zhou, Jing Fan, Yaqian Hu, Qiang Jie, Di Chen, Liu Yang, Zhuojing Luo
Article
Cell Biology
Di Wang, Peiran Zhang, Xiaolong Xu, Jianhui Wang, Dong Wang, Pandi Peng, Chao Zheng, Qing-Jun Meng, Liu Yang, Zhuojing Luo
CELL DEATH & DISEASE
(2019)
Article
Orthopedics
Bo Gao, Jinhua Yin, Xiaolong Xu, Jing Fan, Di Wang, Chao Zheng, Weiguang Lu, Pengzhen Cheng, Jicheng Sun, Dong Wang, Lu Li, Bo O. Zhou, Liu Yang, Zhuojing Luo
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2020)
Article
Cell Biology
Dong Wang, Xin He, Di Wang, Pandi Peng, Xiaolong Xu, Bo Gao, Chao Zheng, Han Wang, Haoruo Jia, Qiliang Shang, Zhen Sun, Zhuojing Luo, Liu Yang
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Review
Cell Biology
Dong Wang, Xin He, Chao Zheng, Chengzhe Wang, Pandi Peng, Chu Gao, Xiaolong Xu, Yachao Ma, Mei Liu, Liu Yang, Zhuojing Luo
Summary: This review summarizes the current knowledge on the impact of endoplasmic reticulum (ER) stress on intervertebral disc degeneration (IDD) and potential therapeutic strategies targeting ER stress for alleviating disc degeneration. Studies have shown potential associations between ER stress and the pathogenesis of IDD, making it a promising therapeutic target.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huanbo Wang, Chao Zheng, Weiguang Lu, Ting He, Jing Fan, Cheng Wang, Qiang Jie, Danny Chan, Kathryn Song Eng Cheah, Liu Yang
Summary: This study examines the role of Hedgehog signaling in the transition from cartilage to bone. The results suggest that Hedgehog signaling plays a crucial role in the differentiation of chondrocytes into osteoblasts, and its regulation is independent of Smo. These findings are of significant importance in understanding bone homeostasis and diseases.
Article
Rheumatology
Pandi Peng, Dong Wang, Xiaolong Xu, Di Wang, Bo Gao, Han Wang, Haoruo Jia, Qiliang Shang, Chao Zheng, Chu Gao, Jianxin Mao, Zhuojing Luo, Liu Yang, Xueyu Hu
Summary: The disruption of the intervertebral disc (IVD) clock due to inflammation is found to be related to the process of intervertebral disc degeneration (IDD). Knockdown of the core clock gene Bmal1 leads to dysfunction of NP cells. The decreased expression of NRF2 and increased inflammatory response, oxidative stress reaction, and apoptosis are observed in NP cells with Bmal1 knockdown. Activation of NRF2 attenuates the dysfunction caused by the disrupted IVD clock and the degenerative process of NP tissues.
ARTHRITIS RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Dong Wang, Pandi Peng, Michal Dudek, Xueyu Hu, Xiaolong Xu, Qiliang Shang, Di Wang, Haoruo Jia, Han Wang, Bo Gao, Chao Zheng, Jianxin Mao, Chu Gao, Xin He, Pengzhen Cheng, Huanbo Wang, Jianmin Zheng, Judith A. Hoyland, Qing-Jun Meng, Zhuojing Luo, Liu Yang
Summary: This study demonstrates that abnormal mechanical loading can dampen the circadian clock in intervertebral discs (IVDs), leading to intervertebral disc degeneration (IDD). Overexpression of the core clock molecule BMAL1 can attenuate compression-induced IDD. Inhibition of the RhoA/ROCK pathway can restore BMAL1 expression and alleviate IDD. These findings highlight the importance of the circadian clock for IVD homeostasis and provide a potential therapeutic strategy for IDD.
Article
Medicine, Research & Experimental
Xiaolong Xu, Di Wang, Chao Zheng, Bo Gao, Jing Fan, Pengzhen Cheng, Baohua Liu, Liu Yang, Zhuojing Luo
