Article
Cell Biology
Yao Huang, Yanhai Feng, Lin Cui, Lei Yang, Qiong Zhang, Junhui Zhang, Xupin Jiang, Xingyue Zhang, Yanling Lv, Jie-Zhi Jia, Dong-Xia Zhang, Yue-Sheng Huang
Summary: The study revealed the relationship between autophagy and necroptosis, showing that LC3 mediated interactions play a crucial role in regulating the necrotic process in cardiomyocytes. Disruption of autophagic flux led to increased necroptosis activation, while intact autophagic flux helped reduce necroptosis occurrence.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Cell Biology
Yaiza R. Varela, Alicia Alonso
Summary: The interaction between LC3/GABARAP proteins and specific lipids plays a crucial role in their function. Cardiolipin and ceramide have been found to be essential for the protein-bilayer interaction.
Article
Biochemistry & Molecular Biology
Yaiza R. Varela, Marina N. Iriondo, Asier Etxaniz, Uxue Ballesteros, L. Ruth Montes, Felix M. Goni, Alicia Alonso
Summary: This study explores the interaction of LC3/GABARAP proteins with phospholipid monolayers and bilayers. It finds that cardiolipin is essential for the protein interaction with bilayers, while ceramide markedly increases binding. Confocal fluorescence microscopy experiments reveal the arrangement of ceramide in the phospholipid bilayers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Shan-Ying Wu, Hung-Tsung Wu, Yi-Ching Wang, Chih-Jen Chang, Yan-Shen Shan, Shang-Rung Wu, Yen-Chi Chiu, Chia-Lang Hsu, Hsueh-Fen Juan, Kai-Ying Lan, Chi-Wen Chu, Ying-Ray Lee, Sheng-Hui Lan, Hsiao-Sheng Liu
Summary: This study reveals that secretory autophagy promotes RAB37-mediated insulin secretion to maintain the homeostasis of insulin and glucose.
Article
Microbiology
Zhaodi Wang, Yukang Wen, Bingqian Zhou, Yaqin Tian, Yaru Ning, Honglei Ding
Summary: The study demonstrated that Mycoplasma hyopneumoniae infection increases autophagic vacuoles in host cells, with enhanced expressions of autophagy marker proteins. Accumulation of autophagosomes promotes the expression of P97 protein and enhances the survival of M. hyopneumoniae in host cells. Inhibition of autophagosome formation reduces the replication of M. hyopneumoniae.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Cell Biology
Jackson O. Gardner, Andrew M. Leidal, Tan A. Nguyen, Jayanta Debnath
Summary: LC3-dependent EV loading and secretion is a secretory autophagy pathway that packages cytosolic cargos into extracellular vesicles (EVs) for secretion. TFRC, a transmembrane protein, is identified as a cargo of this pathway and its packaging and secretion into EVs requires components of the MAP1LC3/LC3-conjugation machinery, ESCRT pathway components, and the small GTPase RAB27A.
Article
Physiology
Dong-Feng Wan, Shan-Shan Pan, Yi-Shan Tong, Yue Huang
Summary: Exercise preconditioning (EP) has been shown to provide cardioprotection through the modulation of LC3 lipidation-associated proteins. The induction phase and protection phase of EP exhibit different alterations in LC3 lipidation-associated proteins, highlighting the importance of autophagy in maintaining cell homeostasis and survival for cardioprotection against exhaustive exercise-induced myocardial injury.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Oncology
Dongqi Li, Chuanchun He, Fan Ye, En Ye, Hao He, Gong Chen, Jing Zhang
Summary: The overexpression of p62 in lung adenocarcinoma bone metastasis is significantly associated with more bone lesions, shorter overall survival rates, and shorter progression free survival rates. In vitro experiments showed that downregulation of p62 inhibited cell migration but not cell proliferation. The expression of p62 in autophagy flux changed inconsistently according to the switch of LC3I to LC3II in different autophagy conditions.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Hao-Chun Chang, Ran N. Tao, Chong Teik Tan, Ya Jun Wu, Boon Huat Bay, Victor C. Yu
Summary: Cells deficient in MOAP1 are hypersensitive to cell death mediated by starvation, and exhibit impaired macroautophagy/autophagy signaling induced by EBSS treatment. MOAP1 interacts with LC3 to promote efficient phagophore closure during starvation.
Article
Cell Biology
Malay Ranjan Biswal, Sreedevi Padmanabhan, Ravi Manjithaya, Meher K. Prakash
Summary: Research has identified proteins that can be secreted outside the cell through unconventional pathways without a known signal, where KKX motif and triacidic amino acid motif play key roles. These findings provide insights into defining a possible signal for unconventional protein secretion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Corey M. Summers, Rudy J. Valentine
Summary: Short bouts of heat can induce a hormetic stress response, but prolonged or excessive exposure may have detrimental effects. This study suggests that 2 hours of heat exposure leads to cell stress and accumulation of autophagasomes in skeletal muscle.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2022)
Review
Cell Biology
Trond Lamark, Terje Johansen
Summary: Selective autophagy is the degradation of specific intracellular components via selective autophagy receptors, which interact with autophagy-related proteins and may or may not depend on ubiquitin labeling of the cargo.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 37
(2021)
Editorial Material
Cell Biology
Marina N. Iriondo, Alicia Alonso
Summary: Recently, we investigated the membrane anchoring and lipidation process of six members of the LC3/GABARAP protein family and their ability to promote membrane tethering and fusion. GABARAP and GABARAPL1 exhibited the highest activities. Differences within the LC3/GABARAP proteins indicated the requirement of a lipidation threshold for tethering and inter-vesicular lipid mixing. The presence of ATG12-ATG5-ATG16L1 (E3) increased and accelerated LC3/GABARAP lipidation and vesicle tethering, but hindered their capacity for inter-vesicular lipid mixing and fusion. Our findings suggest that LC3/GABARAP proteins contribute to phagophore expansion through vesicle tethering and fusion, in addition to the recently described inter-membrane lipid transfer mechanism.
Article
Cell Biology
Shuai Huang, Ye Li, Guihua Sheng, Qingwei Meng, Qian Hu, Xuexiao Gao, Zhiyuan Shang, Qiubo Lv
Summary: SIRT1 promotes autophagy and proliferation of endometrial cancer cells by reducing the acetylation level of LC3.
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Cell Biology
Rosetta Merline, Heiko Roedig, Jinyang Zeng-Brouwers, Chiara Poluzzi, Georg Tascher, Jonas Michaelis, Jaime Lopez-Mosqueda, Andrew Rhiner, Lisa Sophie Huber, Valentina Diehl, Ivan Dikic, Donat Koegel, Christian Muench, Malgorzata Wygrecka, Liliana Schaefer
Summary: The study reveals that ABIN-1 acts as a regulator of mitophagy by forming a complex with LC3B-II, which is crucial for maintaining mitochondrial homeostasis in various physiological and pathological conditions.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)