Journal
SCIENTIFIC REPORTS
Volume 8, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41598-018-24052-4
Keywords
-
Categories
Funding
- Wellcome Trust [093831/B/10/Z, 203249/Z/16/Z, 089703/Z/09/Z]
- German Research Foundation
- Gates Foundation
- UK Engineering and Physical Sciences Research Council, EPSRC [EP/L015889/1, EP/H018301/1]
- UK Medical Research Council, MRC [MR/K015850/1, MR/K02292X/1]
- MedImmune
- Infinitus (China) Ltd.
- Wellcome Trust [093831/B/10/Z] Funding Source: Wellcome Trust
- BBSRC [BB/H023917/1] Funding Source: UKRI
- EPSRC [EP/H018301/1] Funding Source: UKRI
- MRC [G0902243, MR/K02292X/1, MR/K015850/1] Funding Source: UKRI
Ask authors/readers for more resources
Super-resolution single-molecule localization microscopy, often referred to as PALM/STORM, works by ensuring that fewer than one fluorophore in a diffraction-limited volume is emitting at any one time, allowing the observer to infer that the emitter is located at the center of the point-spread function. This requires careful control over the incident light intensity in order to control the rate at which fluorophores are switched on; if too many fluorophores are activated, their point-spread functions overlap, which impedes efficient localization. If too few are activated, the imaging time is impractically long. There is therefore considerable recent interest in constructing so-called 'top-hat' illumination profiles that provide a uniform illumination over the whole field of view. We present the use of a single commercially-available low-cost refractive beamshaping element that can be retrofitted to almost any existing microscope; the illumination profile created by this element demonstrates a marked improvement in the power efficiency of dSTORM microscopy, as well as a significant reduction in the propensity for reconstruction artifacts, compared to conventional Gaussian illumination.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available