Article
Microbiology
Yuzhen Xu, Qingluan Yang, Jingyu Zhou, Feiran Zhou, Yufan Hezhang, Yan Gao, Lingyun Shao, Jichan Shi, Qiaoling Ruan, Wenhong Zhang
Summary: This study evaluates the performance of QFT-GIT and QFT-Plus in diagnosing M. tuberculosis infection in immunocompromised patients, and finds that QFT-Plus may detect more latent tuberculosis infection (LTBI) than QFT-GIT does in patients receiving long-term immunosuppressant therapy. The study contributes to the literature by highlighting the different diagnostic accuracies of QFT-GIT and QFT-Plus in different subpopulations of patients. It suggests that selecting a test with better performance, particularly in high-risk patients, may help improve the management of tuberculosis. The study is also significant for the diagnosis of LTBI.
MICROBIOLOGY SPECTRUM
(2022)
Review
Multidisciplinary Sciences
Chunnian Ren, Jie Tang, Liangfeng Xia
Summary: This study searched PubMed, Embase, and Cochran databases to obtain literature on the diagnosis of osteoarticular tuberculosis (OATB) by Interferon Gamma Release Assays (IGRA) from database establishment to June 2021. The bivariate random effect model was used to summarize the sensitivity, specificity, and accuracy of IGRA in diagnosing OATB, and forest plots and receiver operating characteristic (ROC) curves were used for testing.
Article
Immunology
Xiaojiang Hu, Hongqi Zhang, Yanbin Li, Guang Zhang, Bo Tang, Dongcheng Xu, Mingxing Tang, Chaofeng Guo, Shaohua Liu, Qile Gao
Summary: Preoperative differential diagnosis of spinal tuberculosis is important for clinical management. The QuantiFERON-TB Gold In-Tube (QFT-GIT) test has high sensitivity but low specificity for this diagnosis. The diagnostic efficacy of QFT-GIT can be improved with a new threshold. The intensity of QFT-GIT findings may be related to the duration of the patient's disease.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Shunsuke Akashi, Maho Suzukawa, Keita Takeda, Isao Asari, Masahiro Kawashima, Nobuharu Ohshima, Eri Inoue, Ryota Sato, Masahiro Shimada, Junko Suzuki, Akira Yamane, Atsuhisa Tamura, Ken Ohta, Shigeto Tohma, Katsuji Teruya, Hideaki Nagai
Summary: The new-generation QuantiFERON (QFT)-TB Gold Plus is expected to be useful with a new peptide inducing CD8(+) T cell response. This study found that IL-1RA levels in QFT-GIT supernatant can be a good marker for discriminating active TB from LTBI, showing higher levels in active TB cases.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Kathryn Winglee, Andrew N. Hill, Robert Belknap, Jason E. Stout, Tracy L. Ayers
Summary: Interferon-gamma release assays are important diagnostic tools for tuberculosis infection, but there has been little research on their performance in populations prioritized for tuberculosis testing in the United States. This study found that there is a certain degree of discordance and variability in the results of the QFT and TSPOT tests.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Immunology
Xin Yu, Li-Na Huang, Jun-Chi Xu, Yi-Yan Song, Hui Chen, Cui-Lin Shi, Pei-Jun Tang, Tao Tao, Ye-Han Zhu
Summary: This study analyzed the relationship between silicosis and QFT-GIT assays and found that the sensitivity of QFT-GIT was significantly decreased in silicosis patients. The expression of PD-1 on lymphocytes was found to be higher in silicosis patients with pulmonary tuberculosis compared to normal tuberculosis patients and silicosis only patients. Further analysis revealed a negative correlation between the ratio of PD-1(+)CD4(+)T cells and IFN-gamma, and blocking the PD-1 pathway improved the sensitivity of QFT-GIT in silicosis.
Article
Infectious Diseases
Huiwen Zheng, Jing Xiao, Feina Li, Hao Chen, Deze Li, Yonghong Wang, Yajie Guo, Yuying Chen, Chen Shen
Summary: This study compared the effectiveness of QFT-GIT and XDOT in diagnosing LTBI in children, and identified risk factors associated with indeterminate results.
BMC INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Maryam A. Amour, Christiaan A. Rees, Patricia J. Munseri, Jamila Said, Albert K. Magohe, Mecky Matee, Elizabeth A. Talbot, Robert D. Arbeit, Kisali Pallangyo, C. Fordham von Reyn
Summary: In tuberculosis-endemic regions, the long-term variability of interferon-gamma release assays (IGRA) on healthy subjects is crucial for understanding tuberculosis (TB) vaccine trials based on prevention of infection. By analyzing T-SPOT.TB assays on adolescents, it was found that the definitions of IGRA conversion, reversion, and persistence depend on the frequency of testing. These findings should be considered in the design and interpretation of TB vaccine trials.
Article
Infectious Diseases
Yuanyuan Yu, Yidian Liu, Lan Yao, Yanheng Shen, Qin Sun, Wei Sha
Summary: This study investigated factors influencing false-negative results of the QFT-GIT test in the diagnosis of TB, and personalized cutoff values were established based on different clinical conditions. It was found that age, site of TB, and other factors were significantly correlated with false-negative results. Personalized cutoff values may have superior value in diagnosing active tuberculosis under different conditions.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Multidisciplinary Sciences
Qiang Wang, Fengdan Zhu, Yanjuan Cai, Tao Zhu, Xiaolan Lu
Summary: A nomogram model was established to predict the risk of active tuberculosis (ATB) and differentiate it from inactive tuberculosis (IATB) using the concentration of interferon-γ (IFN-γ) and clinical characteristics. The model was trained and validated in different cohorts and combined with adenosine deaminase (ADA) to improve the performance in ATB cases with pleural effusion (PE). The diagnostic nomogram model showed good sensitivity and specificity in both the training and validation cohorts, and the combination with ADA further improved the performance.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
X. Xiao, G. Da, X. Xie, X. Liu, L. Zhang, B. Zhou, H. Li, P. Li, H. Yang, H. Chen, Y. Fei, G. C. Tsokos, L. Zhao, X. Zhang
Summary: SLE patients with concomitant TB exhibited distinct clinical characteristics compared to controls, including higher frequency of involvement in hematologic, mucocutaneous, and musculoskeletal systems, prior treatment with potent glucocorticoid/immunosuppressive agents, and an increased risk of miliary TB and intracranial TB in patients with lupus before TB infection. Lymphocytopenia was associated with poorer outcomes in SLE/TB + patients.
JOURNAL OF INTERNAL MEDICINE
(2021)
Article
Respiratory System
Yidian Liu, Lan Yao, Feng Wang, Ziyong Sun, Yaoju Tan, Wei Sha
Summary: The TBAg/PHA ratio calculated from T-SPOT.TB assay shows improved performance in the diagnosis of active tuberculosis, with high specificity and sensitivity in distinguishing active tuberculosis from non-tuberculosis patients.
RESPIRATORY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Le Zhang, Jiawei Zhang, Shaoying Yang, Ping Ye, Sheng Chen, Jia Li, Liangjing Lu
Summary: In patients with rheumatic diseases, the T-SPOT test provides more accurate results for screening latent tuberculosis infection compared to QFT, especially in patients with systemic lupus erythematosus. The concordance between the two tests is poor.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2022)
Article
Microbiology
Pei-Jean Feng, Yanjue Wu, Christine S. Ho, Lance Chinna, Andrew Christian Whelen, Angela Largen, Richard Brostrom, Randall Reves, Robert Belknap, Adithya Cattamanchi, Niaz Banaei
Summary: The study found that adding T-Cell Xtend before processing blood samples did not consistently mitigate the loss of spot-forming T cells due to processing delays.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Cell Biology
Xiaoliang Li, Junli Wang, Zhigang Yang, Qiongzhu Song
Summary: This study assessed the diagnostic value of T-SPOT.TB and MRI in suspected osteoarticular tuberculosis patients, finding that a combination of the two methods had higher sensitivity and specificity for the noninvasive diagnosis of osteoarticular tuberculosis compared to using either method alone.