Article
Agronomy
Bentao Xiong, Linfang Wei, Yifan Wang, Jinyu Li, Xin Liu, Yunheng Zhou, Panpan Du, Hao Fang, Johannes Liesche, Yahong Wei, Jisheng Li, Shaolin Chen
Summary: Research on the model fungus Neurospora crassa reveals the important role of cellobiose in regulating the expression and secretion of lignocellulases, involving multiple levels such as proteome and phosphoproteome changes. It offers new insights and potential strategies for improving lignocellulase production.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Biotechnology & Applied Microbiology
Ya Zheng, Bin Mao, Qian Wang, Xin Duan, Meng-Yan Chen, Wei Shen, Chao Li, Yu-Feng Wang
Summary: Knockdown of ocn gene in Drosophila testes resulted in smaller testis without germ cells. Through proteomics sequencing, 606 proteins were identified as significantly changed in expression after ocn knockdown. The down-regulation of ocn disturbed key signaling pathways related to cell survival and differentiation.
Article
Cell Biology
Mingjuan Gu, Xinyu Zhou, Lin Zhu, Yajie Gao, Li Gao, Chunling Bai, Lei Yang, Guangpeng Li
Summary: The study found that Myostatin mutation had no effect on cattle cardiac muscle histologically, but increased glycolysis in biochemical assays. Phosphorylation of PFK was potentiated through the PDE5A-cGMP-PKG pathway, promoting glycolysis in the heart.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liting Ma, Yuran Hao, Xiaorong Liu, Leilei Shao, Hairong Wang, Hao Zhou, Dazhong Zhang, Ting Zhu, Qin Ding, Lingjian Ma
Summary: This study used proteomic and phosphoproteomic analyses to investigate the binucleate anther of a thermosensitive sterile wheat line. It revealed the importance of energy metabolism in male fertility and predicted potential kinase-substrate pairs involved in fertility regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Yongjie Xu, Qiu Han, Chaofeng Ma, Yaling Wang, Pengpeng Zhang, Cencen Li, Xiaofang Cheng, Haixia Xu
Summary: Comprehensive proteomics analysis of sperm from Yorkshire and Duroc boars revealed differential expression of proteins and phosphorylated proteins related to crucial physiological functions such as sperm development, motility, and energy metabolism. Proteins involved in axonemal dynein complex assembly and sperm-egg recognition were found to be significantly higher in Duroc spermatozoa, indicating potential differences in sperm function between the two boar breeds.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Hui Sheng, Yiwen Guo, Linlin Zhang, Junxing Zhang, Manning Miao, Haoyun Tan, Debao Hu, Xin Li, Xiangbin Ding, Guangpeng Li, Hong Guo
Summary: The study revealed that the MSTN gene regulates focal adhesion, PI3K-AKT, and Ribosomal pathway through the COL1A1 gene to promote muscle growth and development in bovine skeletal muscle.
FRONTIERS IN GENETICS
(2021)
Article
Oncology
Jing Hang, Hanqiang Ouyang, Feng Wei, Qihang Zhong, Wanqiong Yuan, Liang Jiang, Zhongjun Liu
Summary: In this study, we comprehensively analyzed the protein and phosphoprotein profiles of chordoma using proteomic and phosphoproteomic approaches. We identified aberrant expression of proteins and phosphorylation events in chordoma tumor tissues, and predicted the involvement of key kinases in the development of chordoma. We also demonstrated the therapeutic potential of targeting these kinases in inhibiting chordoma cell growth.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ze Yan, Xiaojuan Cao, Shouxiang Sun, Bing Sun, Jian Gao
Summary: Hepatic glycolipid metabolism disorder is a key pathogenic factor for many chronic diseases. This study reveals that GAPDH and GSK3B are involved in lipid and glucose metabolism, and inhibition of GSK3B phosphorylation improves glucose tolerance impairment and insulin sensitivity reduction.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Plant Sciences
Devang Mehta, Mina Ghahremani, Maria Perez-Fernandez, Maryalle Tan, Pascal Schlapfer, William C. Plaxton, R. Glen Uhrig
Summary: In this study, protein-level responses to phosphate versus phosphite resupply in phosphate-deprived Arabidopsis thaliana suspension cells were analyzed using label-free proteomics and phosphoproteomics. The main findings included changes mainly at the level of protein phosphorylation, complemented by limited changes in protein abundance. This study sheds light on new aspects of the phosphate starvation response and identifies important targets for further research and potential crop improvement.
Article
Pharmacology & Pharmacy
Zhenpeng Zhang, Yao Zhang, Yuan Li, Songhao Jiang, Feng Xu, Kaixuan Li, Lei Chang, Huiying Gao, Predrag Kukic, Paul Lawford Carmichael, Mark Liddell, Jin Li, Qiang Zhang, Zhitang Lyu, Shuangqing Peng, Tao Zuo, Liz Tulum, Ping Xu
Summary: Protein phosphorylation is a common post-translational modification that plays an important role in regulating cell homeostasis. Quantitative phosphoproteomics can sensitively assess the effects of chemicals on cells, which is beneficial for developing non-animal approaches for chemical safety assessment.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Anna M. Kip, Juan Manuel Valverde, Maarten Altelaar, Ron M. A. Heeren, Inca H. R. Hundscheid, Cornelis H. C. Dejong, Steven W. M. Olde Damink, Benjamin Balluff, Kaatje Lenaerts
Summary: This study investigated the proteome and phosphoproteome changes in the human intestine during ischemia-reperfusion using LC-MS/MS and MSI techniques. The findings revealed alterations in proteins related to intestinal absorption, innate immunity, and extracellular matrix organization, as well as the activity of MAPK and CDK families during IR. The study highlights the importance of complementary use of different MS methodologies in understanding molecular changes during IR.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Lianyuan Tao, Lijun Zhong, Yang Li, Deyu Li, Dianrong Xiu, Juntuo Zhou
Summary: This study utilized proteomics and phosphoproteomics to analyze PDAC tissue samples, identifying multiple pathways strongly associated with PDAC as well as dysregulated proteins and phosphoproteins. Validation at the protein level confirmed these findings, providing insights for PDAC diagnosis and drug development.
Article
Oncology
Lukas Funke, Thilo Bracht, Sebastian Oeck, Karin Schork, Markus Stepath, Sabine Dreesmann, Martin Eisenacher, Barbara Sitek, Alexander Schramm
Summary: This study provides insight into the proteomic and phosphoproteomic dynamics induced by NTRK1 signaling. Activation of NTRK1 leads to upregulation and phosphorylation of Lamin A/C, with aggregation within nucleic foci, regardless of the MYCN status in neuroblastoma cells.
Article
Physiology
Yue Gao, Nan Chen, Xiangle Zhang, Emma Y. Li, Wei Luo, Jie Zhang, Wenqiang Zhang, Sheng Li, Jian Wang, Suning Liu
Summary: Juvenile hormone (JH) regulates insect development and reproduction through intracellular and membrane signaling. Recent studies have shown that JH membrane signaling induces phosphorylation of Met, enhancing its transcriptional activity. JH also induces phosphorylation of Hsp83, promoting Met nuclear import and transcription. Furthermore, 14-3-3 proteins are negative regulators of Met nuclear import through physical interaction with Hsp83.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nelofer Jan, Umer Majeed Wani, Mubashir Ahmad Wani, Hilal Ahmad Qazi, Riffat John
Summary: This study investigated the response of Digitalis purpurea to cold stress in the high altitudes of the Himalayas. The findings demonstrate the plant's tolerance to low temperatures and harsh climatic conditions. Through physiological, biochemical, and proteomic analysis, the study provides insights into the molecular and physiological mechanisms of cold stress response in D. purpurea.
MOLECULAR BIOLOGY REPORTS
(2023)